• Korean Journal of Food Science and Technology
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• v.21 no.3
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• pp.379-386
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• 1989

The soy milk prepared from soy protein concentrate was treated with microbial protease or papain. Growth and acid production by Lactobacillus acidophilus in soy milk containing partially hydrolyzed proteins were investigated. Sensory evaluation of yogurt beverage prepared from protease treated soy milk was also performed. Protease treatment of soy milk enhanced acid production by lactic acid bacteria, particularly in case of microbial pretense and simultaneous treatment by two types of protease showed synergistic effect. pH and number of viable cells were not affected markedly by pretense treatment. Microbial pretense treatment up to 15 minutes or papain treatment up to 45 minutes enhanced acid production, but further treatment up to three hours did not affect the acidity markedly. rho sensory evaluation showed that overall acceptability and taste of soy yogurt beverage were slightly improved when soy milk was treated with microbial pretense of 0.2% or papain of 0.2%. The amount of non-protein nitrogen considerably increased by pretense treatment of 15 minutes and it increased gradually by further treatment up to three hours.

• Korean Journal of Food Science and Technology
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• v.21 no.1
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• pp.92-99
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• 1989

The soy milk prepared from soy protein concentrate was treated with pretense of various concentrations. Growth and acid production by Lactobacillus acidophilus or L. bulgaricus in soy milk containing partially hydrolyzed protein were investigated. Sensory evaluation of soy yogurt beverages prepared from pretense treated soy milk was also performed. The treatment of soy milk with pretense markedly enhanced acid production by lactic acid bacteria, particularly by L. acidophilus, whereas pH and number of viable cells were not .affected by pretense treatment. Protease treatment of 15 minutes greatly enhanced acid production by lactic acid bacteria, but further treatment up to three hours did not affect the acidity markedly. The sensory evaluation showed that overall acceptability of soy yogurt beverages was slightly improved when soy milk was treated with pretense of 0.1%. The amount of non-protein nitrogen was considerably increased by pretense treatment of 15 minutes and it reached the maximum value by treatment of two hours.

• Parasites, Hosts and Diseases
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• v.41 no.3
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• pp.165-169
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• 2003

This study describes the characterization of 80 kDa pretense showing gelationlytic property among three pretenses in the excretory/secretory proteins (ESP) from Toxoplasma gondii. The pretense activity was detected in the ESP but not in the somatic extract of RH tachyzoites. This pretense was active only in the presence of calcium ion but not other divalent cationic ions such as $Cu^{2+},{\;}Zn^{2+},{\;}Mg^{2+},{\;}and{\;}$Mn^{2+}$, implying that $Ca^{2+}$ is critical factor for the activation of the protease. The 80 kDa pretense was optimally active at pH 7.5. Its gelatinolytic activity was maximal at $37^{\circ}C$, and significant level of enzyme activity of the pretense remained after heat treatment at $56^{\circ}C$ for 30 min or $100^{\circ}C$ for 10 min, This thermostable enzyme was strongly inhibited by metal chelators, i.e., EDTA, EGTA, and 11 10-phenanthroline. Thus, the 80 kDa pretense in the ESP secreted by T. gondii was classified as a calcium dependent neutral metalloprotease.

• Textile Coloration and Finishing
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• v.18 no.5 s.90
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• pp.8-14
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• 2006

An earthworm protease, Lumbricus rubellus, was used to improve the dyeing properties of protein fibers such as wool and silk. The optimal condition for the activity of the earthworm pretense was about $40^{\circ}C$ at pH 7. The wool and silk were treated with the protease extracted from an earthworm and the K/S values of the dyed wool and silk were measured using a spectrophotometer in order to compare the dye uptake. The pretense treatment enhanced the dyeing properties of protein fibers without severe changes in mechanical properties. The surface appearances of pretense-treated fibers were observed by microscopy analysis.

• Journal of environmental and Sanitary engineering
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• v.5 no.2
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• pp.103-110
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• 1990

For production of thermophilic and alkaline protease, Bacillus sp. strain AP-5 was isolated from a compost. The production of the protease was reached at maximum for 4 days at $55^{\circ}$ in standing culture. Chitin and Cellulose as carbon source, and Skim Milk as nitrogen source were favorable for the production of the enzyme. Optimal temperature and optimal pH of the enzyme was $55^{\circ}$ and 11, respectively. Metal ion didn't effect on the enzyme activity, the protease was very stable at heat treatment of 30 min at $55^{\circ}$.

• Applied Biological Chemistry
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• v.49 no.4
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• pp.304-308
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• 2006

To extract insoluble proteins from silkworm pupa meal, the meal was treated with pretense produced by Bacillus sp. JH-209. The extraction of insoluble silkworm pupa protein was enhanced at alkaline pHs ranged from 7 to 11 by treatment with the protease. The optimum extraction temperature was $40^{\circ}C$ for in soluble protein treated with pretense. The optimum protease treatment time for extraction of protein was 11 hrs and optimum amount of enzyme treated for extraction of protein was 60 Unit, respectively. The treatment of enzyme extracted more protein than ordinary extraction method without pretense. The foaming capacity, foaming stability, emulsion capacity, and emulsion stability of silkworm pupa meal protein extracted by the treatment of the enzymes increased at all pH ranges. Further more oil absorption as well as water absorption capacities of the protein extracted by the treatment of the enzymes were also increased.

• Korean Journal of Food Science and Technology
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• v.37 no.3
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• pp.377-388
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• 2005

Effects of silkworm powder addition on rheological properties of dough and quality characteristics of bread were investigated. Protein content of silkworm powder was 53,98%, much higher than 12.46% of wheat powder, Crude fiber, fat, and pretense contents of silkworm powder were higher than those of wheat flour, Acid analysis revealed glutamic acid content was highest 4,046.16 mg thus, significant depreciation of breadmaking was expected due to weakened gluten structure or dough. Addition of silkworm powder(optimum at 2%) with pretense inactivated by heat treatment resulted in significant improvement of volume and bread quality, with external and internal scores close to those of the control.

• Plant Resources
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• v.4 no.3
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• pp.192-195
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• 2001

Human immunodeficiency virus (HIV), the causative agent of AIDS, still continues to spread rapidly in the world population, especially in Africa and Southeast Asia. At present, two kinds of therapeutic approaches are used for treatment of AIDS. One is to target HIV reverse transcriptase, which is responsible for the viral genome transcription. The other is to inhibit HIV pretense PR, which is essential for the processing of viral proteins. Drug combinations based on these approaches can reduce the blood virus to an undetectable level. However, a small amount of virus may lurk inside the immune cells in a dormant state. Another major obstacle of long-term treatment of the disease is remarkable mutation in HIV. Most of the clinical chemotherapeutic agents have one or more of these problems. High cost and harmful side-effects further reduced the desirability of these drugs. In the course our studies on development of anti-HIV agents from natural products, we investigated various crude drugs for their inhibitory activity against HIV-induced cytopathic effects (CPE) in culture cells, HIV-pretense (PR), HIV-reverse transcriptase (RT) including ribonuclease H (RNase H), and HIV integrase (INT). In the present paper, some inhibitory substances relating to the development of anti-HIV agents are reported.

• Korean journal of food and cookery science
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• v.7 no.4
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• pp.93-101
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• 1991

The object of this study was to investigate characteristics of kiwifruit protesae and effect of this enzyme on the functionality of casein. The specific activity of crudely prepared kiwifruit pretense on casein was 196.95 units/mg protein, it showed optimum activity at pH 3.0, $60^{\circ}C$. The degree of hydrolysis of casein with pretense treatment steeply increased to 73.5% and 78.9% for 10 and 20 minutes and then reached 84.1% and 89.3% for 1 and 4 hours, respectively. Solubility of non heated control group was 0.2% at pH 4, while the sample groups treated with enzyme for 0, 10 and 20 minutes were 14.5%, 19.2% and 24.0%, respectively. Casein treated with pretense showed marked increase in foam expansion near isoelectric point. However, enzymatically treated groups had lower foam expansion than the control groups. Foam stabilities of enzymatically modified group were lower than those of the control groups at all pH. Emulsifying activity of the non-heated control group was 0% at pH 4, while the groups modified enzymatically for 0, 10, and 60 minutes showed 51.0%, 55.5% and 54.5%, respectively.

• Korean Journal of Poultry Science
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• v.33 no.4
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• pp.303-308
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• 2006

This study was conducted to investigate the effects of mud flat bacteria origin pretense supplementation on e99 qualify, nutrient digestibility and total protein concentration of serum in laying hens. A total of two hundred fifty two laying hens were randomly allocated into three treatments with seven replications for eight weeks. Dietary treatments included 1) CON (basal diet), 2) PRO1 (basal diet + 0.05% pretense) and 3) PRO2 (basal diet + 0.1% pretense). During the entire experimental peripd, hen-day egg production was not affected by treatments (P>0.05). Difference of yolk height was increased in PRO1 treatment compared with CON treatment (P<0.05). Difference of egg weight was increased in PRO2 treatment compared with CON and PRO1 treatments (P<0.05). Shell quality, yolk color unit, haugh unit and egg yolk index were not affected by treatments (P>0.05). DM digestibility was improved in CON and PRO2 treatments compared with PRO1 treatment (P<0.05). N digestibility was improved in PRO2 treatment compared with CON treatment (P<0.05). Total protein concentration in serum were not affected by treatments (P>0.05). In conclusion, mud flat bacteria origin pretense was effective for improving egg weight, yolk height and nutrient digestibility in laying hens.