• 한국화재조사학회논문지
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• 제11권1호
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• pp.1-8
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• 2008

가스연료는 사업체, 가정 등에서 사용이 증가하고 있으며, 이로 인한 사고위험도도 증가추세 이며, 사고원인 또한 다양하게 나타나고 있다. 가스사고를 원인(취급부주의, 제품불량 등), 형태(화재, 폭발, 누출 등), 피해등급(1,2,3,4급), 사상자(사망, 중상, 경상)로 분류하여 설명하였다. 가스연료 중 가정에서 많이 사용되는 LP가스 시설은 가스용기, 압력조정기, 호스, 중간밸브, 연소기로 크게 구분할 수 있으며, 이 구성요소 중의 어떤 부분에서 취급부주의, 제품불량 등의 원인에 의해 화재 및 폭발 등이 발생할 수 있다. 따라서 사고 발생시 과거화재사례에 대한 연구 및 화재조사 시 착안사항을 참고하여 정확한 원인이 규명되어야 한다.

• The Korean Journal of Physiology and Pharmacology
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• 제19권3호
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• pp.203-210
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• 2015

AMP-activated protein kinase (AMPK) is a key regulator of energy metabolism. Previous studies have shown that activation of AMPK results in suppression of cardiac myocyte hypertrophy via inhibition of the p70S6 kinase (p70S6K) and eukaryotic elongation factor-2 (eEF2) signaling pathways. Epigallocatechin-3-gallate (EGCG), the major polyphenol found in green tea, possesses multiple protective effects on the cardiovascular system including cardiac hypertrophy. However, the molecular mechanisms has not been well investigated. In this study, we found that EGCG could significantly reduce natriuretic peptides type A (Nppa), brain natriuretic polypeptide (BNP) mRNA expression and decrease cell surface area in H9C2 cardiomyocytes stimulated with phenylephrine (PE). Moreover, we showed that AMPK is activated in H9C2 cardiomyocytes by EGCG, and AMPK-dependent pathway participates in the inhibitory effects of EGCG on cardiac hypertrophy. Taken together, our findings provide the first evidence that the effect of EGCG against cardiac hypertrophy may be attributed to its activation on AMPK-dependent signaling pathway, suggesting the therapeutic potential of EGCG on the prevention of cardiac remodeling in patients with pressure overload hypertrophy.

• 한국추진공학회지
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• 제2권3호
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• pp.99-106
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• 1998

현재 국내에서 개발되어왔던 중형항공기 후보엔진인 터보팬 엔진의 성능해석과 성능최적화를 위한 제어기법을 연구하였다. 선행된 연구에서 동적모사 및 실시간 선형모사를 수행한 결과 지상 정지조건 하에서 70% 엔진로터 회전수에서 100% 엔진로터 회전수로 급상승하는 경우 고압터어빈 입구온도에서 오버슈트가 발생하여 제한온도인 3105 $^{\cire}R$ 을 넘어감을 확인할 수 있었다 또한 압축기의 서지여유도 협소하여 엔진에 손상을 가져올 수 있다. 이에 본 연구에서는 보다 빠른 가속성능과 함께 엔진 성능의 최적화를 위해 LQR 제어기를 설계하였다. 제어기의 설계를 위해서는 선형모델을 구성해야하며 엔진의 비선형 거동에 보다 근접한 선형화를 위해서는 실시간 선형모사가 요구된다. 선형모델에 필요한 행렬은 자동점에 %의 섭동을 주어 5% 간격으로 구하였으며, 최소자승법을 이용하여 저압 엔진로터 회전수의 함수로 보간하는 방법으로 실시간 선형모사를 수행하였다. 제어변수는 연료유량의 증가속도와 압축기 블리드 공기유량으로 하였으며, 제어 결과 고압 터빈입구온도의 오버슈트를 제거하였으며 최대 압축기 서지여유도 0.55 이하로 확보였다. 비연료소모율도 0.353에서 0.43으로 안정됨을 확인할 수 있었다.

• 한국가스학회지
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• 제5권2호
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• pp.22-29
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• 2001

체적거래제에 의하여 소규모 공동주택에 가스를 공급하기 위하여 적절한 용기설치수의 결정 및 용기교체시기에 대한 검토가 필요하다. 그러나 지금까지 국내에 축적된 자료가 없을 뿐만 아니라 용기 설치수의 결정과 용기수 및 가스사용 형태에 따라 용기교체시 남아 있는 액화석유가스 양에 대한 이론적 검토가 없었다. 따라서 본 연구에서는 자연기화능력, 잔량, 용기의 온도, 용기내부 압력 등을 시뮬레이션으로 살펴보았다. 용기표면으로부터 액화석유가스 용기의 총괄 열전달계수의 변화에 따른 자연기화량 변화는 크지 않으며, 실험으로부터 구한 값은 약 $9{\~}13 kcal/m^2{\cdot}hr{\cdot}^{\circ}C$이었다. 그리고 액화석유가스 용기내부의 압력이 압력조정기의 압력조정 하한계에 도달하였을 때 가스방출 유량은 공기로부터 전달되는 열량과 균형을 이루는 일정한 값으로 급격히 감소하였다. LP가스용기의 자연기화능력은 외기온도 및 프로판의 조성에 따라 증가하였고, 연속사용시간에 따라 초기 급격히 감소하여 일정한 값으로 수렴하였다. 한 가구당 용기수는 자동절체기 사용에 의하여 감소시킬 수 있는 것을 알 수 있다.

• The Journal of Korean Physical Therapy
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• 제20권3호
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• pp.53-59
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• 2008

Purpose: The non-receptor-type protein tyrosine kinase Syk (636 amino acids, 72 kDa) is ubiquitously expressed in hematopoietic stem cells and has been widely studied as a regulator and effector of B cell receptor signaling that occurs in processes such as differentiation, proliferation and apoptosis. However, the mechanism relating Syk and p38 mitogen-activated protein kinases (p38MAPK) by endothelin-1 (ET-1, 21 amino acids) stimulation in muscle cells, especially in the volume-dependent hypertensive state, remains unclear. Methods: In this study, we investigated the relationship between Syk and p38MAPK for isometric contraction and enzymatic activity by ET-1 from rat aortic smooth muscle cells and aldosterone-analogue deoxycorticosterone acetate (DOCA) hypertensive state rats (ADHR). Results: The systolic blood pressure was significantly increased in ADHR than in a control group of animals. ET-1 induced isometric contraction and phosphorylation of p38MAPK, which was increased in muscle strips from ADHR. Increased vasoconstriction and phosphorylation of p38MAPK induced by treatment with 30 nM ET-1 were inhibited by the use of 10${\mu}M$ SB203580, an inhibitor of p38MAPK from ADHR. Furthermore, ET-1 induced isometric contraction and phosphorylation of Syk and p38MAPK, which were increased in the aortic smooth muscle cells. Increased tension and phosphorylation of Syk and p38MAPK induced by ET-1 were inhibited by SB203580 from rat aortic smooth muscle cells. Conclusion: These results, suggest that the Syk activity affects ET-1-induced contraction through p38MAPK in smooth muscle cells and that the same pathway directly or indirectly is associated with volume dependent hypertension. The findings suggest the need to develop cardiovascular disease-specialized physical therapy.

• Biomolecules & Therapeutics
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• 제21권6호
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• pp.487-492
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• 2013

Cytochrome P450 4A11 (CYP4A11) is a fatty acid hydroxylase enzyme expressed in human liver. It catalyzes not only the hydroxylation of saturated and unsaturated fatty acids, but the conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), a regulator of blood pressure. In this study, we performed a directed evolution analysis of CYP4A11 using the luminogenic assay system. A random mutant library of CYP4A11, in which mutations were made throughout the entire coding region, was screened with luciferase activity to detect the demethylation of luciferin-4A (2-[6-methoxyquinolin-2-yl]-4,5-dihydrothiazole-4-carboxylic acid) of CYP4A11 mutants in Escherichia coli. Consecutive rounds of random mutagenesis and screening yielded three improved CYP4A11 mutants, CP2600 (A24T/T263A), CP2601 (T263A), and CP2616 (A24T/T263A/V430E) with ~3-fold increase in whole cells and >10-fold increase in purified proteins on the luminescence assay. However, the steady state kinetic analysis for lauric acid hydroxylation showed the significant reductions in enzymatic activities in all three mutants. A mutant, CP2600, showed a 51% decrease in catalytic efficiency ($k_{cat}/K_m$) for lauric acid hydroxylation mainly due to an increase in $K_m$. CP2601 and CP2616 showed much greater reductions (>75%) in the catalytic efficiency due to both a decrease in $k_{cat}$ and an increase in Km. These decreased catalytic activities of CP2601 and CP2616 can be partially attributed to the changes in substrate affinities. These results suggest that the enzymatic activities of CYP4A11 mutants selected from directed evolution using a luminogenic P450 substrate may not demonstrate a direct correlation with the hydroxylation activities of lauric acid.

• 한국가스학회지
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• 제23권6호
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• pp.25-32
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• 2019

많은 대량소비 가스시설에서는 천연가스를 가스공사의 배관망을 통해 공급받지 못하여 탱크로리를 통해 액화천연가스(LNG)를 가스공사로부터 공급받아 기화시켜 사용한다. 이러한 시설에서 가스 누출로 인한 인명 또는 재산피해를 방지하기 위하여 주입하는 부취제가, 최초지점과 소비지점들에서 어떤 농도로 분포하는지 분석하는 연구를 수행하였다. 일정한 유량이 흐를 때 가스시설내의 배관위치에 따라 부취농도의 변화를 확인하기 위한 분석이 이루어졌다. 시료채취는 알루미늄 재질의 4.5 L 용기를 진공상태로 만든 후, 부취제가 주입되는 정압기와 가스가 사용되는 소비지점에서 시료를 포집하였다. 포집된 가스시료로부터 가스크로마토그래피(Main Body : Agilent 7890A, Detector : ANTEC 7090)를 이용하여 부취제의 농도를 분석하였다. 안전한 가스시설관리를 위하여 대량수요자 시설의 형태별로 부취농도 관리방법의 정립이 필요한 것으로 판단된다.

• Journal of Biosystems Engineering
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• 제36권1호
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• pp.15-22
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• 2011

An experimental variable rate nursery sprayer was developed to adjust application rates for canopy volume in real time. The sprayer consisted of two vertical booms integrated with ultrasonic sensors, and variable rate nozzles coupled with pulse width modulation (PMW) based solenoid valves. A custom-designed microcontroller instructed the sensors to detect canopy size and occurrence and then controlled nozzles to achieve variable application rates. A spray delivery system, which consisted of diaphragm pump, pressure regulator and 4-cycle gasoline engine, offered the spray discharge function. Spray delay time, time adjustment in spray trigger for the leading distance of the sensor, was measured with a high-speed camera, and it was from 50 to 140 ms earlier than the desired time (398 ms) at 3.2 km/h under indoor conditions. Consequently, the sprayer triggered 4.5 to 12.5 cm prior to detected targets. Duty cycles of the sprayer were from 20 to 34 ms for senor-to-canopy (STC) distance from 0.30 to 0.76 m. Outdoor test confirmed that the nozzles were triggered from 290 to 380 ms after detecting tree canopy at 3.2 km/h. The spray rate of the new sprayer was 58.4 to 85.2% of the constant application rate (935 L/ha). Spray coverage was collected at four areas of evergreen canopy by water sensitive papers (WSP), and ranged from 1.9 to 41.1% and 1.8 to 34.7% for variable and constant rate applications, respectively. One WSP area had significant (P < 0.05) difference in mean spray coverage between two application conditions.

• 한국자동차공학회논문집
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• 제23권2호
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• pp.199-207
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• 2015

Solid materials of ammonia sources with SCR have been considered for the application of lean NOx reduction in automobile industry, to overcome complex problems of liquid urea based SCR. These solid materials produce ammonia gas directly with proper heating and can be packaged by compact size, because of high volumetric ammonia density. Among ammonium salts and metal ammine chlorides, calcium ammine chloride was focused on this paper due to low decomposition temperature. In order to make calcium ammine chloride in lab-scale, simple reactor and glove box was designed and built with ammonium gas tank, regulator, and sensors. Basic test conditions of charging ammonia gas to anhydrous calcium chloride are chosen from equilibrium vapor pressure by Van't Hoff plot based on thermodynamic properties of materials. Synthetic method of calcium ammine chloride were studied for different durations, temperatures, and pressures with proper ammonia gas charged, as a respect of ammonia gas adsorption rate(%) from simple weight calculations which were confirmed by IC. Also, lab-made calcium ammine chloride were analyzed by TGA and DSC to clarify decomposition step in the equations of chemical reaction. To understand material characteristics for lab-made calcium ammine chloride, DA, XRD and FT-IR analysis were performed with published data of literature. From analytical results, water content in lab-made calcium ammine chloride can be discovered and new test procedures of water removal were proposed.

• 대한물리치료과학회지
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• 제10권1호
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• pp.158-169
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• 2003

The present study examined that in vivo test is investigated in sham-operated(control group) and aldosterone-analogue deoxycorticosterone acetate (DOCA)-salt hypertensive animals(experimental group) and that the antihypertensive effect was induced by silver spike point(SSP) low frequency electrical stimulation at meridian points(CV-3, -4, Ki-12, SP-6, LR-3, BL-25, -28, -32, -52), specifically, such as diuretic action in 24 hour urine analysis from normal volunteer. The $Na^+$ and $Ca^{2+}$ ions were significantly increased in aldosterone-analogue DOCA-salt hypertensive rats than that in sham-operated rats. However, the $K^+$ ions were significantly decreased in aldosterone-analogue DOCA-salt hypertensive rats than that in sham-operated rats. The current of 1 Hz continue type of SSP low frequency electrical stimulation significantly increased in excretion of urine $Na^+$ and $K^+$ ions from normal volunteer. However, the excretion of $Ca^{2+}$ ion were significantly decreased by SSP electrical stimulation in volunteer. These results suggest that the development of aldosterone analogue-DOCA-salt hypertension is associated with changed $Na^+$, $K^+$ and $Ca^{2+}$ ions of urine. which directly affects blood pressure. Therefore, the hypertension is a risk factor on cardiovascular disease. Moreover, These results demonstrate that the SSP low frequency electrical stimulation, especially current of 1 Hz continue type, significantly regulates $Na^+$, $Ca^{2+}$ and $K^+$ ions from volunteer. Therefore, the SSP low frequency electrical stimulation is a good regulator through a diuretic action of aldosterone-induced hypertension.