• 한국식생활문화학회지
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• 제20권3호
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• pp.352-359
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• 2005

The red ginseng powder was added to Yackwa dough as ratio to 0%, 2%, 4%, 6% and 8%, respectively to know effect of red ginseng powder on Yackwa quality and preservation. The expansion, color, texture and preference characters were investigated at 0,2 and 4 weeks, respectively. The peroxide and acid value were also measured. The 4% addition increased greatly expansion rate. In color test, L value lowed at addition of red ginseng powder and at long storage period. the b value lowed as the addition was increased, but a value was not affected by the addition of red ginseng powder. In mechanical texture test, addition of red ginseng powder had a tendency to show low cohessivness, springness, gumminess while hardness increased. The springness, brittleness and cohessivness decreased while hardness increased at long storage period. In sensory test, surface color, bitterness and red ginseng flavor were recognized strong by addition of red ginseng powder but oily taste, crispness, softness and overall preference were weak. The surface color and red ginseng flavor were strong at long storage period but sweetness, softness and overall preference decreased, respectively. The peroxide value increased at long storage period and decreased after 6 week. The 2% and 4% addition showed lower peroxide value compared to other treatment. The acid value increased at early storage period, but did not change after 4 weeks.

• 대한한의학회지
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• 제37권1호
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• pp.21-33
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• 2016

This study aimed to investigate the stability and biological activities of BHSST decoction depending on the preservation temperature and periods. Methods: BHSST decoction was preserved at room temperatures (R/T, $23{\pm}1^{\circ}C$) or refrigeration ($4^{\circ}C$) for 0, 30, 60 and 90 days. To evaluate the stability of BHSST decoction, pH and sugar content were estimated. In addition, high-performance liquid chromatography (HPLC) analysis was performed to determine marker compounds of BHSST decoction. To evaluate anti-inflammatory effect, nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$) productions were measured in LPS-stimulated RAW 264.7 macrophages. Antioxidant activity was examined using the assays for 3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and 1-1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities. Results: There was no change in pH and sugar content depending on the preservation temperature and periods of BHSST decoction. Among the major components of BHSST, contents of liquiritin, baicalein and wogonin was reduced time-dependently both at R/T and $4^{\circ}C$. Inhibitory effects of BHSST decoction on NO and PGE2 productions were slightly decreased in a time-dependent manner by 90 days of preservation. In addition, BHSST decoction maintained ABTS and DPPH radical scavenging activities by 60 days while significantly reducing the activities in 90 days of preservation at R/T. By contrast, BHSST decoction had no significant change of ABTS and DPPH radical scavenging activities by 90 days at $4^{\circ}C$. Conclusions: Our results suggest that the stability and efficacy of BHSST decoction are maintained for 60 days at $4^{\circ}C$ rather than R/T.

• 한국수정란이식학회지
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• 제28권2호
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• pp.121-125
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• 2013

This study was carried out to investigate the general characteristics of semen such as semen volume, pH, sperm motility and sperm concentration of the semen collected from Shih Tzu dogs (age of 24 to 48 months, weight of 4 to 8 kg) by using the method of digital manipulation of the penis. The effect of preservation temperature and time on motility of fresh semen was also investigated in the present study. Semen was collected for 16 times from 4 male Shih Tzu dogs by multiple ejaculations (four times ejaculation per dog). The average of semen volume, semen pH, sperm motility and sperm concentration of the second fraction containing small volume of the initial third fraction per ejaculation were $2.11{\pm}0.31$ ml, $6.25{\pm}0.07$, $97.59{\pm}1.03%$ and $2.05{\pm}0.14{\times}10^8$ cells/ml, respectively. Average semen volume per ejaculate, semen pH, sperm motility and sperm concentration of the first fraction from the ejaculation were $1.12{\pm}0.15$ ml, $5.99{\pm}0.14$, $16.09{\pm}6.18%$ and $5.16{\pm}2.03{\times}10^5$ cells/ml, respectively. Those of second fraction were $2.07{\pm}0.29$ ml, $6.36{\pm}0.13$, $97.31{\pm}1.36%$ and $2.15{\pm}0.30{\times}10^8$ cells/ml, respectively. Those of third fraction were $2.60{\pm}0.29$ ml, $6.63{\pm}0.08$, $95.72{\pm}1.61%$ and $6.03{\pm}1.83{\times}10^7$ cells/ml, respectively. Sperm motility was significantly higher at $17^{\circ}C$ preservation temperature than at $5^{\circ}C$ or $36^{\circ}C$ during preservation period except 1 h preservation (P<0.05). When preservation temperature was $17^{\circ}C$, sperm motility was $96.69{\pm}1.49%$ at 1 h, $91.38{\pm}1.90%$ at 6 h, $88.38{\pm}2.34%$ at 12 h, $78.13{\pm}4.58%$ at 18 h, $58.44{\pm}8.57%$ at 24 h and $29.56{\pm}5.06%$ at 30 h, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제14권11호
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• pp.1527-1533
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• 2001

Garole is a prolific, rare, less known and small size Indian sheep breed found in low and humid Sunderban region of West Bengal. Although information on stored Garole ram liquid semen upto 24 h is available, but there is a need to further investigate the short-term and long-term preservability of Garole ram semen for extensive utilization of this valuable germplasm by artificial insemination. The aim of the present study was to apply computer-assisted sperm analysis technique for assessing the motion characteristics of Garole ram semen stored (i) in liquid state at refrigeration temperature for short-term preservation upto 48 h and (ii) in frozen state at $-196^{\circ}C$ for long-term preservation after packaging in mini straws. Short-term preservation had a significant effect on motility (p<0.01) as the motility progressively decreased from 90.1% at 0 h to 85.5% and 73.2% after 24 and 48 h of storage, respectively. Although the decline in rapid moving sperms was also significant (p<0.01) on storage but the decrease was more pronounced at 48 h as compared to 24 h of storage period. Storage of chilled semen had also a significant effect on % linearity (p<0.05), % straightness (p<0.01), sperm velocities (p<0.01), amplitude of lateral head displacement (p<0.01) and beat frequency (pO.Ol) of spermatozoa. The replication had a significant effect for all the variables except average path and straight line velocity. However, the interactions of short-term storage and replication were non-significant for most of the variables except % of medium moving sperms, sperm velocities and beat frequency. On long-term preservation of Garole ram spermatozoa under controlled conditions the mean post-thaw recovery of 70.4 and 71.4% motile spermatozoa was achieved having 48.8 and 48.9% of rapidly motile spermatozoa, respectively in both the replicates. The effect of replication on cryopreservation was significant (p<0.05) on amplitude of lateral head displacement and beat frequency, but there was no significant effect on motility, rapidly motile spermatozoa, linearity, straightness and sperm velocities of frozen-thawed spermatozoa. It can be concluded from these results that an average 70% motility can be achieved on storage of Garole ram semen in chilled liquid state upto 48 h or in liquid nitrogen after freezing under controlled conditions in straws. However, further studies are required to evaluate the fertility of short-term and long-term preserved Garole ram semen for extensive use of this prolific sheep breed.

• Journal of Periodontal and Implant Science
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• 제48권4호
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• pp.236-250
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• 2018

Purpose: Resorption of the alveolar bone is an unavoidable consequence of tooth extraction when appropriate alveolar ridge preservation (ARP) measures are not taken. The objective of this trial was to test the hypothesis that dimensional changes in the alveolar bone after tooth extraction would be reduced by inserting an equine collagen membrane and a collagen cone to fill and seal the alveolus (as ARP), in comparison to extraction with untreated alveoli. Methods: In this randomized clinical trial, 31 patients were directly treated with the collagen material after extraction of a tooth from the maxilla (the ARP group). Twenty-nine patients served as the control group. After extraction, no further treatment (i.e., no socket preservation measures) was performed in the control group. Changes in the alveolar process immediately after extraction and after an 8 (${\pm}1$)-week healing period were evaluated 3-dimensionally. Blinded analyses were performed after superimposing the data from the digitalized impressions and surfaces generated by cone-beam computed tomography. Results: Both the ARP and control groups showed a reduction of bone in the alveolar area after tooth extraction. However, significantly less bone resorption was detected in the clinically relevant buccal region in the ARP group. The median bone reduction was 1.18 mm in the ARP group and 5.06 mm in the control group (P=0.03). Conclusions: The proposed hypothesis that inserting a combination material comprising a collagen cone and membrane would lead to a difference in alveolar bone preservation can be accepted for the clinically relevant buccal distance. In this area, implantation of the collagen material led to significantly less alveolar bone resorption. German Clinical Trials Register at www.drks.de, DRKS00004769.

• Journal of Periodontal and Implant Science
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• 제47권4호
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• pp.194-210
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• 2017

Purpose: The aim of the present study was to evaluate the healing of post-extraction sockets following alveolar ridge preservation clinically, radiologically, and histologically. Methods: Overall, 7 extraction sockets in 7 patients were grafted with demineralised bovine bone mineral and covered with a porcine-derived non-crosslinked collagen matrix (CM). Soft tissue healing was clinically evaluated on the basis of a specific healing index. Horizontal and vertical ridge dimensional changes were assessed clinically and radiographically at baseline and 6 months after implant placement. For histological and histomorphometric analysis, bone biopsies were harvested from the augmented sites during implant surgery 6 months after the socket preservation procedure. Results: Clinically, healing proceeded uneventfully in all the sockets. A trend towards reduced horizontal and vertical socket dimensions was observed from baseline to the final examination. The mean width and height of resorption were 1.21 mm (P=0.005) and 0.46 mm (P=0.004), respectively. Histologically, residual xenograft particles ($31.97%{\pm}3.52%$) were surrounded by either newly formed bone ($16.02%{\pm}7.06%$) or connective tissue ($50.67%{\pm}8.42%$) without fibrous encapsulation. The CM underwent a physiological substitution process in favour of well-vascularised collagen-rich connective tissue. Conclusions: Socket preservation using demineralised bovine bone mineral in combination with CM provided stable dimensional changes of the alveolar ridge associated with good reepithelialisation of the soft tissues during a 6-month healing period.

• 한국균학회지
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• 제39권2호
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• pp.141-144
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• 2011

균류의 단기 보존은 일반적으로 한천이 포함된 고체 사면배지에서 이루어진다. 본 연구에서는 2~7년간 PDA 배지를 이용하여 $4^{\circ}C$에서 보관된 담자균류 28종 76개 균주들에 대한 활력을 조사하였다. 균의 생존율은 보존기간이 7년 82%, 6년 86%, 5년 94%, 4년 96%, 3년 94%, 2년 94%로 나타났다. 표고 균주의 배지 부피는 2년 이후부터 감소하였다. 보존된 배지의 pH는 2007년에 5.42였지만 보존기간이 길어질수록 거의 중성 pH에 도달하였다.

• 한국임상수의학회지
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• 제23권1호
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• pp.50-54
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• 2006

To evaluate the effect of punctal occlusion on tear preservation after third eyelid resection, 10 eyes were operated in dogs and observed changes in tear preservation using Schirmer tear test (STT). Punctal occlusion was performed on five healthy dogs with no clinical sings of ocular irritation and no history of dry eye syndrome. The STT was performed for 10 days before surgery, and on the day 0, 1, 3, 5, 7, 9, 11, 13, 17, 21, 25, and 30 after third eyelid resection. The mean STT was $17.4{\pm}0.15$ mm/min($mean{\pm}SEM$) for 10 days before surgery. And, it was $11.5{\pm}0.55$mm/min for 30 days after surgery in puntal occlusion(PO) group. The mean STT was $17.3{\pm}0.32$ mm/min for 10 days before surgery. And, it was more decreased to $6.5{\pm}0.60$ mm/min for 30 days after surgery in control group. The paired t-test was performed. Mean STT after the third eyelid resection was higher in PO group than that of control throughout experimental period (p<0.05). In summary, the effect of thermal punctal occlusion significantly increased on the tear preservation by $28.86{\pm}7.96%$. These results suggest that application of thermal punctal occulsion in dry eye syndrome will promote tear preservation.

• Ocean and Polar Research
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• 제44권4호
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• pp.311-317
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• 2022

Seed germination and seedling growth of a halophyte, Suaeda japonica were examined to measure the effects of seed preservation periods and various soil compositions in the mudflat of Saemangeum, Korea. Seed germination declined from 74.30% to 44.30% as the preservation period increased from 4 months to 12 months. After 14 days of seeding, seed germination was lowest in sandy soil (SS) at 8.67% and highest in earthworm cast+leaf mold (EC+LM) at 67.33%, showing 4~5 times higher than SS. The average lengths of seedlings were 12.20 mm in SS and 42.20 mm in EC+LM, respectively. These results suggest that seed germination and seedling growth enhanced in the soil that has more organic matter. In the experiment mixing Saemangeum soil (SMS), seed germination was from 4.00% in SMS to 88.00% in the soil of SMS+EC+LM after 14 days. Similar growth patterns were observed in the length of seedlings from 5.00 mm in SMS to 49.60 mm in SMS+EC+LM. The present results indicate that a population of Suaeda japonica could be established by stimulating seed germination and seedling growth by the addition of organic matters such as EC, LM, and EC+LM in the Saemangeum mud flat.

• 한국식품저장유통학회지
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• 제21권6호
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• pp.799-807
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• 2014

본 연구는 유자즙의 첨가량을 달리한 마요네즈의 저장기간 중 항산화 활성 및 품질특성 변화를 비교하여 마요네즈의 저장성을 알아보고자 실시하였다. 점도는 유자즙 첨가군이 대조군에 비하여 유의적으로 높은 점도를 나타내었다(p<0.05). 색도에서 유자즙 첨가군의 L값은 대조군에 비하여 낮았으며, 저장기간이 지남에 따라 유의적으로 감소하였다(p<0.05). a값은 b값의 경우, 유자 마요네즈는 유자즙의 첨가량이 많아질수록 b값이 증가하였으며, 저장기간이 지남에 따라 Y-100군 및 L-100군은 b값의 감소를 나타내었다(p<0.05). ${\Delta}E$값은 유자즙 첨가량이 증가할수록 유의적으로 증가하였다(p<0.05). 대조군의 DPPH 라디컬 소거능은 35.90%이었으며, 유자즙 첨가군은 41.74~55.16%로 대조군에 비하여 높게 측정되었고 유자즙 첨가량이 많아질수록 라디컬 소거능이 유의적으로 증가하였다(p<0.05). 모든 실험군의 라디컬 소거능은 저장기간이 지남에 따라 감소를 보였으나, 유자즙 첨가군은 대조군에 비하여 높은 DPPH 라디컬 소거능을 보였다(p<0.05). 유자즙 첨가군의 산가는 대조군에 비하여 유자즙 첨가량이 증가할수록 낮은 산가를 나타내었고, 저장기간이 지남에 따라 산가가 증가하였으나 유자즙 첨가군이 대조군보다 낮은 산가를 나타내었다(p<0.05). 과산화물가도 산가와 같은 결과를 보였는데, 이는 첨가된 유자즙이 마요네즈 안에서 천연 항산화제 역할을 하여 유지의 산패를 억제한 것으로 보인다. 이러한 결과로 마요네즈 제조 시 유자즙의 첨가는 마요네즈의 항산화성을 높이고 저장 중의 산화안정성을 향상시켜 저장성 및 품질개선에 도움이 될 수 있을 것으로 사료된다.