• Advances in nano research
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• 제14권4호
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• pp.391-397
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• 2023

This manuscript describes the one-step eco-friendly green fabrication of silver nanoparticles (AgNPs) through the in-situ bio-reduction of an aqueous solution of silver nitrate using Syzygium aromaticum leaf extract. UV-vis spectroscopy shows a characteristic SPR peak around 442 nm. FTIR spectroscopy showed that the AgNPs were capped with bioactive phyto-molecules. TEM images revealed oval and spherical particles with a mean diameter of ~12.6 nm. XRD analysis revealed crystalline and face-cantered cubic AgNPs. The phytosynthesized AgNPs showed broad-spectrum anti-microbial activity against two foodborne pathogenic bacteria, Listeria monocytogenes and Staphylococcus aureus. The AgNPs showed a prominent ability to inhibit biofilms formed by L. monocytogenes and S. aureus in laboratory conditions through a crystal violet assay. The results suggest that the AgNPs could be a novel nanotool to develop effective antimicrobial and anti-biofilm agents in food preservation.

• 한국식품저장유통학회지
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• 제23권4호
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• pp.560-567
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• 2016

본 연구는 커피의 부산물로 발생되는 커피박으로 침출주를 제조함으로써 커피박의 산업적 활용 가치 재고를 알아보기 위하여 진행되었다. 커피박의 항산화 활성과 생리활성 물질을 분석, 비교하기 위하여 커피와 커피박을 각각 25%와 35% 담금술에 침지하여 CL, CGL를 제조하였다. 제조한 담금술을 대학생을 대상으로 관능평가를 실시한 결과 상대적으로 CGL가 CL에 비해 상대적으로 우수한 기호도를 보였다. 기호도가 높은 CGL와 기호도가 낮은 CL의 항산화 활성 정도를 비교하기 위해 동량의 건조물을 이용해 총 페놀 함량과 항산화 활성 실험을 실시하였다. 총 페놀 함량 분석 결과 35% CGL(1개월)이 $1,000{\mu}g/mL$에서 169.5 mg GAE/g of dried extract으로 가장 높게 나왔고, 항산화 활성 실험 결과 CE와 CL, CGL 모두 유사한 DPPH 라디칼 소거활성과 FRAP 활성을 나타내었다. 따라서 커피 가공 부산물인 커피박에도 DPPH 라디칼 소거활성과 FRAP 활성을 유도하는 phytochemical로서의 생리활성 물질이 잔존하는 것으로 판단된다. 결국 커피박에도 커피와 유사하게 생리활성 물질이 존재하는지 알아보기 위하여 동량의 건조물을 이용해 HPLC 정성 정량 분석을 실시한 결과 CGL(35.0~38.5 mg/g of dried extract)는 CE(34.9 mg/g of dried extract)에 비해 클로로겐산 함량이 0.3~10.5% 가량 더 많았고, CGL(39.2~42.1 mg/g of dried extract)는 CE(35.6 mg/g of dried extract)에 비해 카페인 함량이 10.0~18.2% 가량 더 많은 것으로 나타났다. 본 연구 결과를 종합해볼 때, 커피 가공 부산물인 커피박이 대표적인 생리활성 물질로 알려진 클로로겐산과 카페인을 일정 수준 이상으로 함유하고 있어 커피와 유사한 라디칼 소거활성 등 in vitro 항산화 활성을 가지고 있음을 확인하였다. 더불어 침출주의 경우 커피 대비 기호도도 상대적으로 우수하여 산업적으로 충분히 활용 가능할 것으로 판단된다.

• Parasites, Hosts and Diseases
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• 제32권1호
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• pp.57-60
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• 1994

Thelohanellus kitauei 포자의 운명을 조사하기 위하여 in vitro에서 극사탈출률을 경시적으로 조사하였다. 포자를 0.45% 및 0.9%식염수, 증류수 그리고 Tyrode액에 현탁시켜 $-70^{\circ}C$에 냉동하여 장기간 보존한 바 거의 모든 포자의 활성은 최초 냉동후 1.750일 째까지 초기와 거의 같았다. 0.45% 및 0.9%식염수 그리고 증류수에 현탁시켜 $5^{\circ}C$에 보존한 포자는 각각 1,628일, 1,614일 그리고 1,721일, 항생제를 첨가한 위의 현탁액에서는 각각 1,628일, 1.614일 그리고 1,714일에 모든 포자가 사멸하였다. 한편, Tyrode액에 현탁시킨 것은 모두 235일에 모든 포자가 사멸하였다.

• Journal of Microbiology and Biotechnology
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• 제16권8호
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• pp.1301-1305
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• 2006

The development of rapid, infallible, and sensitive methods of detecting foodborne pathogens has received much impetus in recent years owing to an increased public awareness of the health hazards. For the rapid and simultaneous detection of these foodborne pathogens, a multiplex PCR method was developed. Yersinia enterocolitica, Staphylococcus aureus, and Shigella spp. are bacteria of concern because of their specific growing condition that enables them to live at low temperatures. In order to detect each pathogenic bacterium, specific primers from Y. enterocolitica, St. aureus, and Sh. flexneri were selected and validated successfully. To apply this method to food stored at low temperature, Y. enterocolitica, St. aureus, and Sh. flexneri were artificially inoculated in lettuce and incubated for enrichment. The multiplex PCR assays were able to simultaneously detect three pathogens, and the presence of three bands was observed at initial inoculation levels of approximately 1$\times$10$^1$ CFU/g in lettuce. Therefore, this method could be used for simultaneous detection of Y. enterocolitica, St. aureus, and Shigella spp. contaminated in lettuce during cultivation, transportation, preservation, and storage.

• 한국농공학회지
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• 제38권4호
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• pp.99-109
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• 1996

This study, a preliminary one to construction of standardized rural resources evaluation system, aimed at catching the valuing structure of rural and urban residents cn rural resources through interview. The questionnaire employed in our surveys was focussed on; opinion about the advantages and disadvantages of living in rural areas, usual impression on rural life style, viewpoint on preservation of cultural heritages and conservation of natural environments, priority item listing for improvement/development of rural village. Generally, regardless of interviewee's personal characteristics, the peaceful and comfortable life in the lovely/clean environment was indicated as a representative advantage of rural life. However, in minor aspects, there were recognizable differences by their living and thinking patterns, so, a deep and systematic study should be progressed to harmonize or integrate the pluralistic valuing trends on rural affairs in future. The valuing structure on rural resources from this study showed much more varieties according to age-and occupation-groups, while young generation strongly hold self-orii-ented and ambiguous thinking. Because this trend will grow more deeply and widely in future, the resoruces evaluation system for rural purposes should be established as a rational base for decision-making on rural development strategies.

• 동아시아식생활학회지
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• 제19권2호
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• pp.256-264
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• 2009

This study investigated the status of foodservice operations and perceived performance of management for school dietitians in the Kyunggi area. According to the results, the perceived performance of dietitians for cooking management presented an overall average of $3.90{\pm}0.61$, and carrying out examination and preservation of food was the highest among all sectors with $4.83{\pm}0.49$. The area of cross contamination marked a total average of $4.15{\pm}0.67$, and the perceived performance of serving management was lower than the sanitary management of cooking and cross contamination with $3.64{\pm}0.75$. The most recognized cause on foodborne illness was food materials (44.6%). Sanitary level, the temperature of most foods, and the personal hygiene of workers were also important causes of foodborne illness.

• Molecules and Cells
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• 제39권8호
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• pp.639-644
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• 2016

Discovery of non-invasive diagnostic and predictive biomarkers for acute rejection in liver transplant patients would help to ensure the preservation of liver function in the graft, eventually contributing to improved graft and patient survival. We evaluated selected cytokines and chemokines in the sera from liver transplant patients as potential biomarkers for acute rejection, and found that the combined detection of IL-10, IL-17, and CXCL10 at 1-2 weeks post-operation could predict acute rejection following adult liver transplantation with 97% specificity and 94% sensitivity.

• Agribusiness and Information Management
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• 제11권2호
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• pp.16-24
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• 2019

BRIS has been established by the Ministry of Agriculture, Food and Rural Affairs since 2011 under Article 21 of the Act on the Preservation, Management and Utilization of Agricultural Life Resources. It is an information service that provides information related to agricultural life resources in connection with the Rural Development Administration, the Forest Service, and Korea Seed & Variety Service, Agricultural Resources and Agricultural and Livestock Quarantine Headquarters. The purpose of this study is to evaluate how the users assess the information system quality (information quality, system quality) for the current Bio Resource Information Service (BRIS). Ultimately, the structural equation modeling analyzes the causal relationship between each variable and how it responds to the user's continuous use intention. As a result, information quality among information system quality and information quality among system quality used as the main variables for the evaluation of BRIS were statistically positively influenced by users' perceived usefulness, while system quality It has been analyzed to have a positive effect on the sustained use intention. The user's continuous use intention is positively influenced by the information system quality and perceived usefulness at a statistically significant level. Especially, the perceived usefulness plays a role in mediating the user's continuous use intention.

• 펄프종이기술
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• 제43권3호
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• pp.73-79
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• 2011

The annals of Joseon Dynasty is one of the UNESCO's Memory of the World Register. For the safety preservation of wax applied volumes of the annals of Joseon Dynasty, the aging behavior of beeswax and beeswaxed Hanji with $CO_2$, $O_3$, $SO_2$ and $NO_2$ gas has been evaluated. The weight loss of beeswaxed Hanji after aging under ozone gas were increased because of strong acidity of ozone. The acid value and relative intensity of carbonyl groups in beeswax were stabilized with aging time. The physical strength of dewaxed Hanji after ozone aging were rapidly decreased by aging time. The crystallinity of dewaxed Hanji were stabilized at all aging times.

• 한국축산식품학회지
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• 제37권4호
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• pp.477-485
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• 2017

Novel, effective methods to control and prevent spoilage and contamination by pathogenic microorganisms in meat and meat products are in constant demand. Non-thermal pasteurization is an ideal method for the preservation of meat and meat products because it does not use heat during the pasteurization process. Atmospheric pressure cold plasma (APCP) is a new technology for the non-thermal pasteurization of meat and meat products. Several recent studies have shown that APCP treatment reduces the number of pathogenic microorganisms in meat and meat products. Furthermore, APCP treatment can be used to generate nitrite, which is an essential component of the curing process. Here, we introduce the effectiveness of APCP treatment as a pasteurization method and/or curing process for use in the meat and meat product processing industry.