• Food Science and Preservation
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• v.7 no.2
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• pp.201-206
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• 2000

${\beta}$-Galactosidase was extracted and purified from strawberry. The purified ${\beta}$-Galactosidase from strawberry was investigated their physicochemical characteristics. ${\beta}$-Galactosidase was purified 25.74 fold from strawberry. The purification procedure include ammonium sulfate fraction, acetone powder treatment and gel and ion exchange chromatography. Yield of the enzyme purification was 18.11%. The purified enzyme has native molecular weight of 116,000 dalton. Vmax value and Km value of ${\beta}$-Galactosidase were 0.077 mM ONPG/ml/15mim and 1.75x10-2mM, respectively. The optimum temperature and pH of ${\beta}$-Galactosidase were 43$^{\circ}$C and pH 4.0, respectively. The ${\beta}$-Galactosidase activity was stable below 50$^{\circ}$C and at pH 4.0 to pH 6.0. Among the metal ions Ca and Mg were did not affect, whereas K, Cu and Zn show a little effect on the enzyme activity. The ${\beta}$-Galactosidase activities were inhibited by treatment with EDTA and SDS.

• Journal of architectural history
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• v.10 no.2 s.26
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• pp.55-71
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• 2001

This study reviewed the preservation status of the Early Western Style Architecture in Korea based on the on-site research of church buildings which are registered as National Cultural Properties. I have investigated 21 church buildings regarding the frequency, content and concept of restoration. The results are summarized as follows First, the churches have been restored extensively in every 17 years on the average. Second, the main restorations are replacement of bricks, painting, waterproofing, repair of roof and timber. And the main changes are extension of plan, removal of Interior posts, and replacement of floor. Third, the main concept of restorations are historical restoration(62%), critical restoration(23%), stylistic restoration(14%). Forth, the category of restoration have been changed, that is extending of incompletion (1st stage), functional improvement and extension of the span of life(2nd stage), improvement of facilities(3rd stage), restoration to the original state and conservation(4th stage). Fifth, the issues of repairing techniques are revival and replacement of weathered bricks, the removal of painting, and structural reinforcement. This study will be able to be used as the fundamental materials to improve the techniques of restoration of Early Western style masonry buildings.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.1
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• pp.124-130
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• 2008

Streptococcus (Strep.) agalactiae is one of the major pathogens of bovine mastitis and is the main cause of subclinical infection. This study attempted to develop a rapid PCR diagnosis procedure using milk samples collected on filter paper disks. Chromatographic filter paper was employed as the preservation media and kept at room temperature for one to four weeks. The revival rate of Strep. agalactiae kept on dried filter paper disks was affected by the pretreatment preservation time. The revival test suggested that not all the bacteria in artificially contaminated milk samples on the filter paper disks could be recovered. After that, a PCR based on the 16-23S intergenic spacer region of Strep agalactiae was performed. The results distinguished the strep. agalactiae from major pathogens of bovine mastitis at a $2{\times}10^2$ colony forming units (CFU)/ml level, which showed similar sensitivity to the results from liquid milk samples. The results also showed that milk samples collected on filter paper disks could be kept at room temperature for one to four weeks with little negative effect on sensitivity and specificity. The field test showed that the diagnostic sensitivity and specificity was 96.15% and 98.60%, respectively. In conclusion, the protocol will provide a rapid and economic procedure for the detection of bovine mastitis.

• Journal of Korean Society of Rural Planning
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• v.13 no.4
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• pp.23-30
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• 2007

The purpose of this paper is to estimate economic value of multi-functionality roles including landscape and so on, which grassland in Jeju possesses, using Dichotomous Choice Contingent Valuation Method (DC-CVM). Grassland is life cultural heritance of regional residents in Jeju. It has functions like development of favorable landscapes, maintenance of cultural heritage, and recreation/relaxation. Expectation from regional visitors shows that the existence and inheritance value of grassland is higher than we expected. According to the research that we had done from the people that had agreed to contribute grassland. moreover the public commercial value of grassland was not only $397{\sim}419$ billion won but also it will increase an economic gain in every year in Jeju. Therefore, preserving grassland that has multi-functional roles and values can lead to huge economic value in the future in Jeju. Moreover, farm households which are located in the grassland conservation area would be supported $15{\sim}31$ billion won in every year for grassland conservation but it is only $4{\sim}8%$ of the economic value of grassland i.e. $3,971{\sim}4190$ billion won. Thus, grassland preservation policy and plan can raise green-tour commercial value of Jeju area as a long-term inside measurement. Those will have to be propelled continuously.

• Food Science and Preservation
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• v.12 no.1
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• pp.90-94
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• 2005

To poodnce a value added of mulberry, this study was optimized the condition for manufacture of wine using mulberry. On fermentation of mulberry wine, the best yeasts were Saccharomyces cerevisiae JBS 30 and JBS 33, and optimum composition of medium was crushed mulberry juice of $50\%$, sugar of $24\;^{\circ}brix,\;Na_2S_2O_5\;of\;0.02\%$. The content of alcohol after fermentation of 10 days at $25^{\circ}C$ was $11.2\%$. Sensory evaluation showed that color, taste and odor of mulberry wine were acceptable, and Saccharomyces cerevisiae JBS 30 was not difference compared to JBS 33.

• Food Science and Preservation
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• v.4 no.2
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• pp.147-153
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• 1997

Oriental melon has been increased in production amount, but its processed food was not made in spite of the fact that shelf-life of the fresh fruit is short. This study was carried out to develop a dried product with no use sulfur treatment. Fresh melons were peeled, cut into 6 pieces, and soaked to the following pretreatments soaking in sugar syrup(SS), sodium chloride(SC), ascorbic acid(AA) and sodium polyphosphate(SP). After preatreatments the melon pieces were dried by hot air drying at 5$0^{\circ}C$ for 9 and 12 hours, and the dried melons were air blown at $25^{\circ}C$ for 1 day. The dried samples were evaluated for moisture content, texture, rotor, and sensory quality. The moisture content of dried melons soaked in SS and SC were lower than those that were soaked in AA and SP after hot air drying. The melons dried for 12 hours were high in hardness, gumminess, chewness and adhesiveness and excellent in sensory evaluation compared to 9 hours. The "L" value of SS was higher and the "a" value was lower in Hunter color. And SS treatment inhibited browning of the dried melon and improved sensory characteristics in color, flavor, texture and taste. Sucrose concentration had no significant effect on color, hardness and sensory score in banal product. The combination of SS with SP represented a highly effective antibrowning treatment for the dried melon and the product was kept in good color for 3 months at room temperature.at room temperature.

• Korean Institute of Interior Design Journal
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• v.18 no.3
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• pp.93-101
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• 2009

Nowadays, the constructions of dams are being issued by the public due to the preservation and protection of nature considered more important than development. These social phenomenon can not be evitable due to arguing the both attitudes, preservation and development of nature. However, There are possibilities to make an agreement with the compromise between the two sides. The common goals of the attitudes are concerning human beings and their life quality. In this atmosphere, the exhibition hall of dams is very important to lead the agreement of both groups because the spaces not only provide contents to compromise by showing positive factors of dams, but present condition of using water as natural resources. The purpose of this study is based on the effect of the applied media in an exhibition hall as presenting education and enlightenment factors. To examine the effect, this study categorizes the existing media by communication types firstly. Secondly, based on the categorization, existing media of each dam exhibition hall are analyzed by numerical way. Thirdly, the general characteristics of applied media are defined by integrating the spatial contents and media. The data from the study are effective to provide the basic data for following design works for the exhibition space.

• Food Science and Preservation
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• v.5 no.2
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• pp.138-143
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• 1998

The stowage effects of Satsuma mandarin(Citrus unshiu Marc. var. miyagawa) were investigated by selecting various pretreatmnents; non-tested, wax-coating, paper packaging, 0.02mm cryovac film packaging. Weight loss of film packaging was the lowest among that of others, but decay radio was increased highly at late stages of storage. Soluble solids, moisture content of peel and total sugar were maintained almost constant, but acid content, vitamin C and firmness were reduced gradually during storage at room temperature. For 100 days storage, losses from weight and decay of ton-treated, paper packaging, wax-coating and film packaging were 15.9%, 18.5%, 17.4% and 12.9%, respectively. Acid content was decreased loom 1.28% to 0.81∼0.91% after 100 days storage. Ethylene evolution was increased in a degree after 65 days storage, and the amount was increased repidly afer 115 days. it seemed to be derived from decayed fruits and physiological activities. CO2 content of inner part of fruits was increased between 40∼100 days after storage. Optimum storage period of early variety of Satuma mandarin was regarded for 100days on the basis of appeareance and taste.

• Journal of the Korean Home Economics Association
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• v.34 no.5
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• pp.183-196
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• 1996

The purposes of this study were to review the effects of textiles production on the environment, and to investigate the environmental consciousness and environmental preservation behavior of textile producers. This study was conducted by reference analysis and empirical research. To develope theoretical framework of dimensions of environmental behavior, references concerned were analyzed. And for empirical study, researcher developed a questionnaire based on the free writing by producers and references. The questionnaire included problems about environmental consciousness, environmental behavior, demographic variables, and environmental variables. 135 questionnaires were used for final data analysis. ANOVA and factor analysis were used. The results were as follows: First, the level of global environmental problem consciousness was relatively high. The conscious level of water pollution caused by the waste water from textile mills was the highest, and that of desertation of mountain caused by timber cutting was the lowest. The effects of textile dyers and finishers on the environment were the highest, and that of designers were the lowest. Second, the results of reference analysis showed that the dimensions of textile producers environmental behavior were resource and energy saving, solid waste reduction, and green product production. And the results of empirical study were resource and energy saving, resource reuse or recycling, solid waste reduction, and green product production, and total variances was 62.3%. The practice was the lowest. Third, global environment problem consciousness, environment problem consciousness caused by the textile life-cycle concerned, and clothing seperate-collection or not at residing place were effective on environmental behavior, and 52.45% of environmental behavior was explained with above variables.

• Korean Institute of Interior Design Journal
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• v.16 no.6
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• pp.181-188
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• 2007

The existing art museums existed for preservation and exhibition of collected material and the work which has artistic value. But today the art museum is not just for preservation and exhibition. It takes charge of public education as opened place to public. Further, it wants to be the art museum that the visitors who visit the art museum can enjoy and participate. The role change of like this is judged with the inevitable result which follows in many social demand. And increase with interest of leisure and culture life and continued demand for education, the role of modern art museum is becoming wider. Llike this, to accomplish these roles, space configuration of the modern art museum is changing. In the future, to meet more social demands and to solve flexibly, the modern art museum must concern more spacial efforts. Therefore, to grasp the change and the aspect of modern art museum, this study analyzes the Jeonbuk Province Art Museum, the Gyeongnam Art Museum and the Gyeonggido Museum of Art, They are most recently opened museum after 2000. To grasp space configuration of these museums, space syntax was used. And with the result which is analyzed, as grasps the problem and suggest an alternative idea, it can suggest advanced and proper direction.