• Korean Journal of Veterinary Service
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• v.45 no.3
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• pp.237-242
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• 2022

The simultaneous analysis of mycotoxins using LC-MS/MS, a food official analysis method, was applied with compound feed for pets with high consumer preferences. In this study, the linearity of all calibration curves showed good linearity of 0.99 or more. and both the accuracy (recovery rate) and precision (repeatability) criteria of the concentration range for each mycotoxin in the National Agricultural Products Quality Management Service's Validation and Verification Guidelines were met. And as a result of analyzing FAPAS QCM in the same way, it was assesed that the z-scores of Aflatoxin B₁, Ochratoxin A, Zearalenone, and Fumonisin B₁, were within ±2 range. This study showed that the application of the food official analysis method to compound feed for pets is suitable.

• International Journal of Automotive Technology
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• v.5 no.2
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• pp.95-108
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• 2004

Lanekeeping assistance has the potential to save thousands of lives every year by preventing accidental road departure. This paper presents experimental validation of a potential field lanekeeping assistance system with quantitative performance guarantees. The lanekeeping system is implemented on a 1997 Corvette modified for steer-by-wire capability. With no mechanical connection between the hand wheel and road wheels the lanekeeping system can add steering inputs independently from the driver. Implementation of the lanekeeping system uses a novel combination of a multi-antenna Global Positioning System (GPS) and precision road maps. Preliminary experimental data shows that this control scheme performs extremely well for driver assistance and closely matches simulation results, verifying previous theoretical guarantees for safety. These results also motivate future work which will focus on interaction with the driver.

• Nuclear Engineering and Technology
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• v.37 no.2
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• pp.167-176
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• 2005

We developed the PERUPS system to aid the on-line performance analysis for the turbine cycle of the YongGwang 3 and 4 nuclear power plants. Procedure of measurement validation is included in the performance calculation to obtain heat balance. Precision of on-line performance calculation is increased via practical modifications of standard calculation algorithms based on the PTC (Performance Test Code). The proposed system also provides useful Web-based aids for performance analysis, including performance data management, a graphic viewer for heat balance and turbine expansion lines, and synthesized reports of performance.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2019.06a
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• pp.252-254
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• 2019

본 논문은 합성곱 기반의 Single Shot Multibox Detector(SSD) [1] 의 구조를 이용하여 다양한 스케일의 얼굴들을 잘 검출하도록 하였다. 얼굴 검출은 물체 검출과는 다르게 얼굴의 높이와 너비의 비율이 다소 일정하고 크기가 작은 경우가 많은데, 이에 맞게 얼굴 검출이 용이하도록 anchor의 스케일, 비율, 크기를 변경하였다. 특징점 추출 네트워크는 깊은 네트워크의 최적화를 용이하게 하는 skip connection을 이용한 ResNet-50 [2] 기반을 사용하였다. 다양한 크기, 조명, 환경, 각도의 얼굴들을 포함하는 영상들로 이뤄진 Wider Face[3] 데이터 셋의 easy validation set으로 실험한 결과 0.782과 hard validation set에서 0.611의 average precision을 보였다.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2020.07a
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• pp.40-42
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• 2020

본 논문에서는 합성곱 기반의 얼굴 검출기 Dual Shot Face Detector (DSFD)에 대하여, 특징점 맵의 희소화와 채널 프루닝 목적 함수를 사용하여 네트웍 경량화를 수행하였다. 특징점 맵을 희소화하기 위해 L1 목적 함수를 사용했고, 특징점 맵의 채널 프루닝을 하기 위해 채널 최대값이 가장 낮은 채널들의 합을 최소화 시키는 목적함수를 적용했다. 기존의 신경망은 특징점 맵 희소화 비율이 45%였고 두 목적 함수를 적용했을 때 69.67% 로 희소화 비율이 높아진 것을 확인했다. 얼굴 검출 성능을 다양한 조명, 크기, 환경, 각도, 표정의 얼굴들을 포함하는 영상들로 이뤄진 Wider Face 데이터 셋으로 실험한 결과, average precision은 하락 했고 easy validation set에서 0.9257, hard validation set에서 0.8363 였다.

• Journal of the Korean Society of Food Science and Nutrition
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• v.43 no.11
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• pp.1791-1795
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• 2014

The objective of this study was to compare two extraction methods for determination of vitamin K1 (phylloquinone) in vegetables. In addition, analytical method validation parameters such as accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), and linearity were calculated to ensure the method's validity. Vitamin K1 was quantified by reversed-phase HPLC using post-column derivatization and fluorescence detection ($Ex{\lambda}=243nm$, $Ex{\lambda}=430nm$). Higher analytical values were observed using solvent extraction compared to those from the enzyme extraction method. The results from the method validation showed high linearity in the calibration curve with a coefficient of correlation ($R^2$) of 0.9994. The LOD and LOQ were 0.1335 and 0.2784 ng/injection volume ($50{\mu}L$), respectively. The inter-day precision and inter-day precision were 2.0% and 2.1%, respectively. Overall recovery was close to 100% (n=5). The phylloquinone contents ranged from 9.42 to $1,212.57{\mu}g/100g$. Our study provides reliable data on the phylloquinone contents in commonly consumed vegetables in Korea.

• Clinical and Experimental Pediatrics
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• v.50 no.2
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• pp.143-150
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• 2007

Purpose : This study was conducted to validate enzyme immunoassay (EIA) for the quantitative measurement of human IgG antibodies specific for Haemophilus influenzae type b (Hib) capsular polysaccharide. Method : We evaluated specificity, repeatability, intermediate precision, accuracy, lower limit of quantification (LLOQ), and stability to validate standardized EIA for the quantitative measurement of human anti-polyribosylribitol phosphate (PRP) IgG antibodies. Results : The results indicated that this EIA showed specificity to HbO-HA antigen and repeatability and intermediate precision were within acceptance criteria (repeatability: $CV{\leq}15%$, intermediate precision: $CV{\leq}20%$). The EIA-derived results from this laboratory were equivalent to those obtained by the standard radioactive antigen binding assay (RABA) for quantitation of anti-PRP antibodies in the 28 sera. Spiking recovery result was within acceptance criteria ($100{\pm}20%$). The precision and accuracy of samples in LLOQ were from -14.7 to -4.7% in nominal values, which were within acceptance criteria (precision: $CV{\leq}25%$, accuracy: ${\pm}25%$). Freeze-thaw stability and short term temperature stability were within ${\pm}20%$ of acceptance criteria. Conclusions : The EIA which is performed at the Center for Vaccine Evaluation and Study Ewha Medical Research Institute, is an appropriate serologic assay which can be used for quantitation of anti-PRP IgG antibodies in human sera.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.11
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• pp.1682-1686
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• 2015

This study was conducted to establish an HPLC analysis method for determination of marker compounds as part of materials standardization for development of health functional food materials from pear pomace. The quantitative determination method of caffeic acid and chlorogenic acid as marker compounds of pear pomace extract (PPE) was optimized by HPLC analysis using a C18 column ($5{\times}250mm$, $5{\mu}m$) with a 0.2% elution gradient of acetic acid and methanol as the mobile phase at a flow rate of 0.8 mL/min and detection wavelength of 330 nm. The HPLC/UV method was applied successfully to the quantification of marker compounds in PPE after validation of the method with linearity, accuracy, and precision. The method showed high linearity of the calibration curve with a coefficient of correlation ($R^2$) of 0.9999, and limit of detection and limit of quantification were $1.14{\mu}g/mL$ (caffeic acid) and $1.61{\mu}g/mL$ (chlorogenic acid) as well as $4.9{\mu}g/mL$ (caffeic acid) and $4.9{\mu}g/mL$ (chlorogenic acid), respectively. Relative standard deviation values from intra- and inter-day precision were less than 3.1% (caffeic acid) and 4.0% (chlorogenic acid), respectively. Recovery rates of caffeic acid and chlorogenic acid at 12.5, 25, and $50{\mu}g/mL$ were 93.66~106.32% and 97.33~105.68%, respectively. An optimized method for extraction of caffeic acid and chlorogenic acid in PPE was established through diverse extraction conditions, and the validation indicated that the method is very useful for evaluation of marker compounds in PPE to develop a health functional food material.

• Journal of Food Hygiene and Safety
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• v.29 no.4
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• pp.312-315
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• 2014

The present study demonstrated the development and validation of the method for the quantification of phenol in food using gas chromatography coupled with mass spectrometry (GC-MS). After spiking of internal standard (Phenol-$d_5$) to food, those samples were extracted with organic solvent mixture (acetone : dichloromethane = 1 : 1, v/v) using ultra sonic extractor and cleaned by gel permeation chromatography (GPC) technique. The amount of phenol was determined by GC/MS. To validate the developed method, we evaluated parameters were the selectivity, linearity, accuracy, precision, and recovery. To demonstrate the selectivity of the method, blank samples of rice, corn, and fish(mackerel) were prepared and subjected to GC-MS analysis. To verify the linearity of the method, six different standard concentrations of phenol at 0.01, 0.05, 0.1, 0.5, 1 and 2.5 mg/kg were evaluated. The correlation coefficient ($r^2$) of calibration curve was 0.9999. The recovery rate for phenol standard calculated by internal standard method were 82.2~101.5% for samples fortified with 0.25, 0.50, and 1.0 mg/kg, respectively. Also the repeatability and reproducibility for validation of precision were 0.2~5.5%. According to the result of the validation, this established method was suitable for AOAC guideline. The limit of detection (LOD) for phenol analysis were 0.03~0.1 mg/kg, and the limit of quantification (LOQ) were 0.1~0.3 mg/kg. Therefore, we established the optimal analysis method for determination of phenol in food using GPC and GC/MS.

• Journal of Food Hygiene and Safety
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• v.34 no.1
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• pp.52-57
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• 2019

Method development and validation of decursin for the standardization of Angelica gigas Nakai as a functional ingredient and health food were accomplished. The quantitative determination method of decursin as a marker compound of aerial parts of Angelica gigas Nakai extract (AAGE) was optimized by HPLC analysis using a C18 column ($3{\times}150mm$, $3{\mu}m$) with 0.1% TFA in water and acetonitrile as the mobile phase at a flow rate of 0.5 mL/min and detection wavelength of 330 nm. The HPLC/PDA method was applied successfully to quantification of the marker compound in AAGE after validation of the method with linearity, accuracy, and precision. The method showed high linearity in the calibration curve at a coefficient of correlation ($R^2$) of 0.9994 and the limit of detection and limit of quantitation were $0.011{\mu}g/mL$ and $0.033{\mu}g/mL$, respectively. Relative standard deviation (RSD) values of data from intra- and inter-day precision were less than 1.10% and 1.13%, respectively. Recovery of decursin at 0.5, 1, 5 and $10{\mu}g/mL$ were 92.38 ~ 104.11%. These results suggest that the developed HPLC method is very useful for the determination of marker compound in AAGE to develop a health functional material.