• Title/Summary/Keyword: pre-strain

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Evaluation of Streptomyces padanus IA70-5 Strain to Control Hot Pepper Anthracnose (Colletotrichum acutatum) (고추 탄저병 (Colletotrichum acutatum) 방제를 위한 Streptomyces padanus IA70-5의 평가)

  • Chi, Tran Thi Phuong;Choi, Okhee;Kwak, Youn-Sig;Son, Daeyoung;Lee, Jeung Joo;Kim, Jinwoo
    • Journal of agriculture & life science
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    • v.46 no.3
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    • pp.37-45
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    • 2012
  • To select bacterial strains with antifungal activity against an anthracnose fungal disease causing damage severely on hot pepper, previous isolates obtained from plant root samples were screened. Among 457 isolates, IA70-5 isolate was finally selected and identified as Streptomyces padanus based on 16S rDNA sequence analysis. Strain IA70-5 is non-pigmenteous, non-mobile, and filamentous. S. padanus IA70-5 inhibited effectively the mycelium growth, spore germination, and appressorium formation of Colletotrichum acutatum in vitro. The results of this study demonstrated that IA70-5 strain, especially applied on fruit of hot pepper, decreased disease incidence 90% for pre-inoculation before pathogen treatment. Taken together, S. padanus IA70-5 strain is a promising biological control agent to control of a major fungal pepper disease, anthracnose.

Cardiac Phenotyping of SARS-CoV-2 in British Columbia: A Prospective Echo Study With Strain Imaging

  • Jeffrey Yim;Michael Y.C. Tsang;Anand Venkataraman;Shane Balthazaar;Ken Gin;John Jue;Parvathy Nair;Christina Luong;Darwin F. Yeung;Robb Moss;Sean A Virani;Jane McKay;Margot Williams;Eric C. Sayre;Purang Abolmaesumi;Teresa S.M. Tsang
    • Journal of Cardiovascular Imaging
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    • v.31 no.3
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    • pp.125-132
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    • 2023
  • BACKGROUND: There is limited data on the residual echocardiographic findings including strain analysis among post-coronavirus disease (COVID) patients. The aim of our study is to prospectively phenotype post-COVID patients. METHODS: All patients discharged following acute COVID infection were systematically followed in the post-COVID-19 Recovery Clinic at Vancouver General Hospital and St. Paul's Hospital. At 4-18 weeks post diagnosis, patients underwent comprehensive echocardiographic assessment. Left ventricular ejection fraction (LVEF) was assessed by 3D, 2D Biplane Simpson's, or visual estimate. LV global longitudinal strain (GLS) was measured using a vendor-independent 2D speckle-tracking software (TomTec). RESULTS: A total of 127 patients (53% female, mean age 58 years) were included in our analyses. At baseline, cardiac conditions were present in 58% of the patients (15% coronary artery disease, 4% heart failure, 44% hypertension, 10% atrial fibrillation) while the remainder were free of cardiac conditions. COVID-19 serious complications were present in 79% of the patients (76% pneumonia, 37% intensive care unit admission, 21% intubation, 1% myocarditis). Normal LVEF was seen in 96% of the cohort and 97% had normal right ventricular systolic function. A high proportion (53%) had abnormal LV GLS defined as < 18%. Average LV GLS of septal and inferior segments were lower compared to that of other segments. Among patients without pre-existing cardiac conditions, LVEF was abnormal in only 1.9%, but LV GLS was abnormal in 46% of the patients. CONCLUSIONS: Most post-COVID patients had normal LVEF at 4-18 weeks post diagnosis, but over half had abnormal LV GLS.

Effect of Planned Nursing Intervention on the Stress, the Maternal Role Strain, and the Maternal Role Performance of Mothers of Premature Infants (계획된 간호 중재가 미숙아 어머니의 스트레스, 모성 역할 긴장과 역할 수행에 미치는 영향)

  • Joung Kyoun -Hwa
    • Child Health Nursing Research
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    • v.5 no.1
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    • pp.70-83
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    • 1999
  • The birth of a premature infant is distressing for its parents. The parents of a premature infant experience stress according to the infant's physical appearance and behavior, the environment of the neonatal intensive care unit (NICU) , and the alteration in the parental role. Especially, a mother of a premature infant feels distressed even after the discharge of the infant : therefore, she has difficulties in maternal role performance. The main purpose of this study is to identify the effects of the planned infant care information program in order to lower the stress level for mothers of premature infants caused by the birth and hospitalization in NICU of premature infants, to reduce the maternal role strain, and to promote the maternal role performance after the infants' discharge. This study employed two methods of research at the same time : quasi -experimental non-equivalent pre and post test to compare : non-equivalent post test to compare. The total number of subjects was 19 who were assigned to the research program : 12 mothers of premature infants at the NICU at the Ch university hospital and 7 at the NICU at the Y general hospital located in Chounju city. The data were collected for 79 days from August 18 to November 5, 1998. The questionnaire method was applied for the data collection, and the measures used in this study were Parental Stressor Scale : NICU(Miles, 1993), the Maternal Role Strain Measures ( Hobbs, 1968 ; Steffensmeier, 1982) , and Self Confidence Scale (Pharis, 1978). Research procedure is as follows : after preliminary examination, the experimental subjects, the mothers of premature infants at the Nl CU at Ch university hospital were provided with slide films and information developed by the researcher based on existing documents and data. It took two 60-minute sessions a week for two weeks, and the mothers' stress level was measured using the same instrument twice one week and two week after the infants' hospitalization. The stress level of the contrast subjects, the mothers at Y general hospital was measured during the same period. The experimental subjects were provided with booklets on matters that require attention after the infants' discharge and on developmental project, and they were educated to play the maternal role in person for 2-3 hours a week : breast-feeding, burping a baby, and changing diapers. One week after the infants' discharge, the maternal role strain and the maternal role performance were examined in two groups of the subjects. The analysis of collected data was done using descriptive statistics including real numbers, percentages, averages, and standard deviations. Mann-Whitney test ; x² test ; Repeated Measures Analysis of Variance ; ANCOVA Spearman's rho correlation coefficients. The results on this study were as follows. (1) The examination of the same quality showed that there were no differences in the general and obstetrical characters between the two groups. However, in terms of the characters of premature infants. just right after their birth, the infants at the contrast group weighed more than those at the experimental group(U=16.5, p=.02), and the former was in mother's womb longer than the latter(U=15.5, p=.02). (2) The stress level of the mothers provided with the plannned nursing intervention program became lower as time passed compared to the others'(F=16.61, p=.00) Even when the influence of weight at birth and the length of gestation was removed among the premature infants' characters, the mothers' stress levels made a statistical difference 2 weeks after the infants' hospitalization depending on treatment (F=8.00, p=.01) (3) The maternal role strain of the mothers provided with the planned nursing intervention program was lower than the others'(U=2.0, p=.00). Even when the influence of weight at birth and the length of gestation was removed among the premature infants' characters, the maternal role strain levels made a statistical difference 2 weeks after the infants' hospitalization, depending on treatment(F=14.72, p=.00). (4) The maternal role performance level of the mothers provided with the planned nursing program was higher than the others'(U=.0, p=.00). Even when the influence of weight at birth and the length of gestation was removed among the premature infants' characters, the mothers' stress levels made a statistical difference 2 weeks after the infants' hospitalization, depending on treatment(F=8.00, p=.01). (5) The correlation between a mother's stress level 2 weeks after her infant's hospitalization, the maternal role strain and the maternal role performance were compared : the stress and the maternal role strain were statistically irrelevant to each other(r=.33, p=.12) : the stress was found to be in inverse proportion to the maternal role performance(r=-.53, p=.02). The maternal role strain was in inverse proportion to the maternal role performance as well(r=-.50, p=.00). In conclusion, for the mothers provided with the planned nursing intervention program, their stress level was getting lower as time passed during the infants' hospitalization, their maternal role strain reduced when they took care of their infants after their discharge, and their maternal role performance level was high compared to the other mothers. Besides, the lower the stress level of mothers of premature infants was during the infants' hospitalization, the higher the maternal role performance after their discharge was. The lower maternal role strain was, the higher the maternal role performance was as well. These results of the study suggested that the nursing intervention program for the mothers of premature infants developed by the researcher would be effectively applied to nursing practice, and it would be a foundation for the development of this kind of program.

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The Application of Maturity Method on Whitetopping Construction (성숙도 개념을 이용한 Whitetopping 포장의 현장 적용)

  • Jung, Jong-Suk;Cho, Yoon-Ho;Lee, Kang-Won
    • International Journal of Highway Engineering
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    • v.7 no.1 s.23
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    • pp.83-92
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    • 2005
  • Maturity method is a non-destructive method for estimating in-place concrete strength as a function of time and temperature. The main objective is to use maturity method determining joint sawing and traffic opening time for whitetopping construction in Korea. Another objective is to investigate the influence of air temperature in the correspondence to slab temperature and maturity value. For determining the joint sawing and traffic opening time, thermachron i-button and strain gage were inserted in the fresh concrete in the slab and a maturity value was calculated at desired times. In-place strength was then estimated from a pre-established relationship between maturity values and compressive strength. The results showed that there are significant differences between the estimated strength obtained from maturity curve and in-place concrete strength. The reasons are that the gain of in-place concrete strength was influenced by several factors in the field such as curing conditions, air temperature, and wind speed etc. Also, the results showed that air temperature had significant influence on slab temperature and maturity value The slopes of maturity curves exponentially decrease as air temperature decreases. This means that maturity value sharply dropped as air temperature decreases.

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Thermoelastic effect on inter-laminar embedded delamination characteristics in Spar Wingskin Joints made with laminated FRP composites

  • Mishra, P.K.;Pradhan, A.K.;Pandit, M.K.;Panda, S.K.
    • Steel and Composite Structures
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    • v.35 no.3
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    • pp.439-447
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    • 2020
  • This paper presents two sets of full three-dimensional thermoelastic finite element analyses of superimposed thermo-mechanically loaded Spar Wingskin Joints made with laminated Graphite Fiber Reinforced Plastic composites. The study emphasizes the influence of residual thermal stresses and material anisotropy on the inter-laminar delamination behavior of the joint structure. The delamination has been pre-embedded at the most likely location, i.e., in resin layer between the top and next ply of the fiber reinforced plastic laminated wingskin and near the spar overlap end. Multi-Point Constraint finite elements have been made use of at the vicinity of the delamination fronts. This helps in simulating the growth of the embedded delamination at both ends. The inter-laminar thermoelastic peel and shear stresses responsible for causing delamination damage due to a combined thermal and a static loading have been evaluated. Strain energy release rate components corresponding to the Mode I (opening), Mode II (sliding) and Mode III (tearing) of delamination are determined using the principle of Virtual Crack Closure Technique. These are seen to be different and non-self-similar at the two fronts of the embedded delamination. Residual stresses developed due to the thermoelastic anisotropy of the laminae are found to strongly influence the delamination onset and propagation characteristics, which have been reflected by the asymmetries in the nature of energy release rate plots and their significant variation along the delamination front.

Performance evaluation according to the forming method during production of bellows for LNG carriers II - Comparison of low cycle fatigue characteristics - (LNG 선박용 벨로우즈의 제작시 성형방법에 따른 성능 평가 II - 저주기 피로 특성 비교 -)

  • Kim, Pyung-Su;Kim, Jong-Do
    • Journal of Advanced Marine Engineering and Technology
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    • v.40 no.7
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    • pp.593-598
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    • 2016
  • Static tests and low-cycle fatigue tests were conducted to analyze the characteristics of the bellows for LNG vessels according to the forming methods. The cycle life of bellows was tested based on the specified cyclic life, 80000 cycles, to analyze the difference in characteristics between pre-and post-test data by measuring the strain and stress of each convolution of formed bellows. The low-cycle fatigue test was conducted using a strain gauge that was attached to the convolution of bellows. Formed bellows were placed on the structural test device which was equipped with a hydraulic system and was capable of moving in the x-y direction. Data was measured and processed by a multi recorder. Through the static test and low-cycle fatigue tests results, the difference between the cycle life of bellows formed by mechanical methods and of those formed by hydraulic methods was investigated. Moreover, the cause of difference in cyclic life according to forming methods was performed.

A New Salt-Tolerant Thermostable Cellulase from a Marine Bacillus sp. Strain

  • dos Santos, Yago Queiroz;de Veras, Bruno Oliveira;de Franca, Anderson Felipe Jacome;Gorlach-Lira, Krystyna;Velasques, Jannaina;Migliolo, Ludovico;dos Santos, Elizeu Antunes
    • Journal of Microbiology and Biotechnology
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    • v.28 no.7
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    • pp.1078-1085
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    • 2018
  • A salt-tolerant cellulase secreted by a marine Bacillus sp. SR22 strain with wide resistance to temperature and pH was purified and characterized. Its approximate mass was 37 kDa. The endoglucanase, named as Bc22Cel, was purified by ammonium sulfate precipitation, gel filtration chromatography, and extraction from the gel after non-reducing sodium dodecyl sufate-polyacrylamide gel electrophoresis. The optimal pH value and temperature of Bc22Cel were 6.5 and $60^{\circ}C$, respectively. The purified Bc22Cel showed a considerable halophilic property, being able to maintain more than 70% of residual activity even when pre-incubated with 1.5 M NaCl for 1 h. Kinetic analysis of the purified enzyme showed the $K_m$ and $V_{max}$ to be 0.704 mg/ml and $29.85{\mu}mol{\cdot}ml^{-1}{\cdot}min^{-1}$, respectively. Taken together, the present data indicate Bc22Cel as a potential and useful candidate for industrial applications, such as the bioconversion of sugarcane bagasse to its derivatives.

Study on Local Buckling of District Heating Pipes Using Limit State Design (한계상태 설계법을 이용한 지역난방 열배관의 국부좌굴 연구)

  • Kim, Joo-Yong;Lee, Sang-Youn;Ko, Hyun-Il;Cho, Chong-Du
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.34 no.12
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    • pp.1829-1836
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    • 2010
  • The district heating system distributes the heat generated from a cogeneration plant to wider locations. In this process, the district heating pipe (DHP) is subjected to internal and external loadings. The internal loadings are generally caused by the operating conditions such as water temperature and internal pressure. Frictional interactions between the pipes and the soil contribute to the external loadings. Thus, investigation of the mechanisms of failure of DHPs will help to guarantee both mechanical stability and heating efficiency. In this study, we investigate the local buckling of DHPs using limit state design (LSD). Two methods are considered: the use of the limit state for the width-thickness ratio and the use of the limit state for the strain. The results are used to confirm that the DHP is stable under local buckling. Finally, we suggest a minimum preheating temperature for avoiding local buckling.

Optimum topology design of geometrically nonlinear suspended domes using ECBO

  • Kaveh, A.;Rezaei, M.
    • Structural Engineering and Mechanics
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    • v.56 no.4
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    • pp.667-694
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    • 2015
  • The suspended dome system is a new structural form that has become popular in the construction of long-span roof structures. Suspended dome is a kind of new pre-stressed space grid structure that has complex mechanical characteristics. In this paper, an optimum topology design algorithm is performed using the enhanced colliding bodies optimization (ECBO) method. The length of the strut, the cable initial strain, the cross-sectional area of the cables and the cross-sectional size of steel elements are adopted as design variables and the minimum volume of each dome is taken as the objective function. The topology optimization on lamella dome is performed by considering the type of the joint connections to determine the optimum number of rings, the optimum number of joints in each ring, the optimum height of crown and tubular sections of these domes. A simple procedure is provided to determine the configuration of the dome. This procedure includes calculating the joint coordinates and steel elements and cables constructions. The design constraints are implemented according to the provision of LRFD-AISC (Load and Resistance Factor Design-American Institute of Steel Constitution). This paper explores the efficiency of lamella dome with pin-joint and rigid-joint connections and compares them to investigate the performance of these domes under wind (according to the ASCE 7-05), dead and snow loading conditions. Then, a suspended dome with pin-joint single-layer reticulated shell and a suspended dome with rigid-joint single-layer reticulated shell are discussed. Optimization is performed via ECBO algorithm to demonstrate the effectiveness and robustness of the ECBO in creating optimal design for suspended domes.

Expression of Antibody Genes Specific for Human Hepatitis-B Virus in Transgenic Tabacco Plants (형질전환된 담배에서 사람 B형 간염바이러스 항체 유전자의 발현)

  • Seok Yoon KWON;Shin Je KIM;Hyo Jeong HONG;Moon Hi HAN;Chang Ho CHUNG;Ho Sul LEE;Kyung Hee PAEK
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.6
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    • pp.353-356
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    • 1994
  • Chimeric kappa chain and gamma chain cDNA clones (pCKS2 and pCHS2) of a monoclonal antibody specific for pre-S2 surface antigen of human hepatitis-B virus were ligated into Xbal site of plant expression vector pBKS-1. Plasmid DNA containing each of the chimeric gene were then mobilized from E, coli to Agrobacterium tumefaciens strain LBA4404. The chimeric antibody genes were then introduced into tobacco by Ti plasmid-mediated transformation. The putative Transformants were selected on medium containing kamaycin sulfate. Shoots that formed on shoot induction medium were analyzed by Western blot analysis for the expression of kappa-chain or gamma-chain genes. The Western blot analyses clearly showed that the introduced genes were stably expressed in transgenic plants.

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