• Title/Summary/Keyword: pre-mix

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A Simple and Reliable Method for Preparation of Cross-Contamination-Free Plant Genomic DNA for PCR-Based Detection of Transgenes

  • Hwang, Seon-Kap;Kim, Young-Mi
    • BMB Reports
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    • v.33 no.6
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    • pp.537-540
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    • 2000
  • A simplified but reliable method was developed for the polymerase chain reaction (PCR)-based detection of genetically modified (GM) plants. The modified CTAB (mCTAB) method enabled us to prepare a high quality of genomic DNA from several hundred plant leaf samples in one day. Using DNA samples prepared from seven dicots and two monocots, approximately 1.75-kb regions spanning 17 S to 25 S ribosomal RNA genes were successfully amplified in a 2X PCR pre-mix containing BLOTTO. Further fidelity assessment of the mCTAB method by PCR analysis with Roundup Ready soybean (RRS) and non-RRS plants showed that the DNA samples prepared alternately from each of two lines were evidently free of cross-contamination. These results demonstrate that the mCTAB method is highly recommended for the rapid detection of transgenes in large numbers of leaf samples from diverse transgenic plants.

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The Properties of Foamed Concrete Slurry Using Bottom Ash According to the Methods of Mixing of Foam (기포혼입방법에 따른 바텀애쉬를 사용한 기포 콘크리트의 특성)

  • Kang Cheol;Kang Gi Woong;Kawg Eun Gu;Cho Sung Hyun;Kwon Gi Ju;Kim Jin Man
    • Proceedings of the Korea Concrete Institute Conference
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    • 2004.11a
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    • pp.529-532
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    • 2004
  • As the purpose of this study is a research of series to obtain fundamental data on the development of the product of foamed concrete using bottom ash for various applications in the field, the main purpose is the light weight of product of concrete. In this experiment, method of mixing of foam is very important because it control specific gravity and strength of the product. As the test results, it was found that regardless of mixing method the grower the concentration of foaming agent the lower the specific gravity and the compressive strength of the specimen especially pre-foaming method. From a strength point of view, we knew that mix-foaming method and steam curing is efficient to obtain a adequate compressive strength of foamed concrete.

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Properties of Foamed Concrete According to Concentrations of Synthetic Type Foaming Agents (합성 기포제 희석 농도에 따른 기포콘크리트의 특성)

  • Choi, Ji-Ho;Shin, Sang-Chul;Park, Hyo-Jin;Kim, Ji-Ho;Jeong, Ji-Yong;Kim, Jin-Man
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2011.05a
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    • pp.123-125
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    • 2011
  • Pre-foaming that has been used in this study is using to control and guarantee quality, but the optimum mix proportion and regulation are not definite. Therefore, this study investigated properties of foamed concrete according to concentrations of foaming agent to improve usability of foamed concrete. Synthetic foaming agent such as AES(Alkyl Ether Sulfate) and AOS(Alpha Olefin Sulfonate) are used to make foam with 1, 3, and 5% concentrations. We found that the flow of foam concrete increases when foam concentration is high and AES is more flowable than AOS. Density and compressive strength increase when foam concentration is low.

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Resource Eestimation of Grover Algorithm through Hash Function LSH Quantum Circuit Optimization (해시함수 LSH 양자 회로 최적화를 통한 그루버 알고리즘 적용 자원 추정)

  • Song, Gyeong-ju;Jang, Kyung-bae;Seo, Hwa-jeong
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.31 no.3
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    • pp.323-330
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    • 2021
  • Recently, the advantages of high-speed arithmetic in quantum computers have been known, and interest in quantum circuits utilizing qubits has increased. The Grover algorithm is a quantum algorithm that can reduce n-bit security level symmetric key cryptography and hash functions to n/2-bit security level. Since the Grover algorithm work on quantum computers, the symmetric cryptographic technique and hash function to be applied must be implemented in a quantum circuit. This is the motivation for these studies, and recently, research on implementing symmetric cryptographic technique and hash functions in quantum circuits has been actively conducted. However, at present, in a situation where the number of qubits is limited, we are interested in implementing with the minimum number of qubits and aim for efficient implementation. In this paper, the domestic hash function LSH is efficiently implemented using qubits recycling and pre-computation. Also, major operations such as Mix and Final were efficiently implemented as quantum circuits using ProjectQ, a quantum programming tool provided by IBM, and the quantum resources required for this were evaluated.

Optimal Mix Design of High-Performance, Low-Heat Self-Compacting Concrete (고성능 저발열 자기충전 콘크리트의 최적 배합설계)

  • Kim, Young-Bong;Lee, Jun-Hae;Park, Dong-Cheon
    • Journal of the Korea Institute of Building Construction
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    • v.22 no.4
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    • pp.337-345
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    • 2022
  • The foundation of high-rise concrete building in coastal areas generally must be installed in an integrated manner, not separately, in order to prevent defects caused by stress on the upper and lower parts of the mounting surface and to manage the process smoothly. However, when performing integrated punching, there is a concern that temperature stress cracks may occur due to hydration heat. Due to the large member size, it is difficult to make a sufficient commitment, so it is necessary to mix concrete with high self-charging properties to ensure workability. In this research, the amount of high-performance spray and admixture used was adjusted as experimental variables to satisfy this required performance. Through the analysis of the results for each blending variable, it was found that the unit quantity was 155kg/m3 and the cement ratio in the binder was 18%, and the target values of the pre-concrete properties and compressive strength were satisfied. A four-component binder(18% cement, 50% slag fine powder, 27% fly ash, 5% silica fume) was used.

An Experimental Study on the Required Performances of Roof Concrete Placed in the In-ground LNG Storage Tank (지하식 LNG 저장탱크의 지붕 콘크리트의 요구성능에 관한 실험적 연구)

  • Kwon, Yeong-Ho
    • Journal of the Korea Concrete Institute
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    • v.25 no.3
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    • pp.339-345
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    • 2013
  • This study is to derive from the required performances and the optimum mix proportion of the roof concrete placed in the in-ground LNG storage tank with a capacity of 200000 $m^3$, and propose the actual data for site concrete work. The concrete placing work without sliding and segregation in the fresh concrete condition is very important because the slope of domed roof is varied in the large range by its curvature. Also the control of hydration heat and the strength development at test ages are classified with massive section about 1.4 m thick and considered to the pre-stressing work and removal of air support after concrete placing work. Considering above condition, slump range is selected $100{\pm}25$ mm under the slope $20^{\circ}$ and $150{\pm}25$ mm over the slope $20^{\circ}$ s until 60 minutes of elapsed time. Also, the roof concrete is satisfied with compressive strength range including design strength at 91 days (30 MPa), pre-stressing work at 7 days (10 MPa), air support removal work at 21 days (14 MPa). Replacement ratio of limestone powder is determined by confined water ratio test and main design factors include water-cement ratio (W/C), sand-aggregate ratio and dosage of admixture. As test results, the optimum mix proportion of the roof concrete used low heat cement is as followings. 1) Replacement ratio of limestone powder 25% by confined water ratio test 2) Water-cement ratio 57.8% 3) Sand-aggregate ratio 42.0%. Also, test results for the adiabatic temperature rising test is satisfied with its criteria and shown the lower value compared to preceding storage tank (TK-13, 14). These required performances and the optimum mix proportion is to apply the actual construction work.

Evaluation of Landfilling Method of Organic Sludge from Mix of Pre-treated Organic Sludge and Municipal Solid Waste (전처리된 유기성오니와 생활폐기물 혼합에 따른 유기성오니 매립방법 평가)

  • Ko, Jae-Young;Phae, Chae-Gun;Do, In-Hwan;Park, Joon-Seok
    • Journal of Korean Society of Environmental Engineers
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    • v.30 no.3
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    • pp.278-285
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    • 2008
  • This research was performed to evaluate the landfilling method of organic sludge from mix of pre-treated organic sludge (OS) and municipal solid waste(MSW). Organic sludges were dried, composted, and solidified as pre-treatment and the OS and MSW were mixed in ratios of 2 to 8 and 4 to 6. Approximately 1,800$\sim$2,500 L of landfill gas(LFG) was generated in the lysimeter with solidified-OS, which was higher than 1,150$\sim$1,650 L of the dried- and composted- ones. Maximum H$_2$S concentration was found in the following order : Composted-20(80 ppmv) > Composted-40(55 ppmv) > Dried-20(30 ppmv) > Dried-40 $\fallingdotseq$ Solidified-20 $\fallingdotseq$ Solidified-40 (20 ppmv). BOD$_5$ at initial leachate generation period was 38,000 mg/L for Composted-40, 28,000 mg/L for Dried-40, 26,000 mg/L for Dried-20, 21,000 mg/L for Composted-20 and Solidified-40, and Solidified-20 for 17,000 mg/L. In the final period of experiment, BOD$_5$ was low as 300$\sim$500 mg/L in the lysimeter with solidified-OS and MSW and showed 2,000$\sim$3,500 mg/L in dried- and composted- ones. As the results, landfilling by mix of solidified-OS and MSW was evaluated as the most appropriate method for biodegradable organics. Direct landfilling of OS is permitted for landfill site with CDM facility. Therefore, mixed landfilling of solidified-OS and MSW should be considered for much more LFG generation as methane.

Study for Sequential Application of Herbicide to Establish an Efficient Weed Control in Red Pepper Field (고추 밭 잡초 관리를 위한 제초제 체계 처리법 개발)

  • Min, Yi-Gi;So, Yoon-Sup
    • Weed & Turfgrass Science
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    • v.5 no.4
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    • pp.213-218
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    • 2016
  • Timely application and the choice of herbicides are crucial for red pepper production since the yield is significantly reduced by weed occurrence. Experiments were conducted to provide efficient weed control methods in red-pepper fields. The results suggest the followings: 1) in the field of prevalent grass weeds, application of pendimethalin EC as pre-emergence herbicide after transplanting followed by tank-mix with pendimethalin and fluazipfop-P-butyl EC as post-emergence at 3-5 leaf stage of Digitalia species gave a good control for 80 days without crop injury, 2) as for grass and other weeds occurrence, sequential application of tank-mix with glufosinate-ammonium SL and pendimethalin at 30 days after transplanting (DAT) followed by glufosinate-ammonium. at < 20 cm of weed height with 30 days' interval provided better weed control than 2-time application of glufosinate-ammonium. single application for 80 days in this trial. 3) To prevent from drifting of non-selective herbicide spray mist into red-pepper at furrow application, glufosinate-ammonium. should be applied at 15 cm of spray nozzle height at 20 DAT (18 cm tall of red pepper), and the spray nozzle should be placed below 30 cm above ground to keep spray drift minimum to red pepper with > 40 cm plant height at 40 DAT.

Impacts of DRG Payment System on Behavior of Medical Insurance Claimants (DRG 지불제도 도입에 따른 의료보험청구 행태 변화)

  • Kang, Gil-Won;Park, Hyoung-Keun;Kim, Chang-Yup;Kim, Yong-Ik;Ha, Beom-Man
    • Journal of Preventive Medicine and Public Health
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    • v.33 no.4
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    • pp.393-401
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    • 2000
  • Objectives : To evaluate the impacts of the DRG payment system on the behavior of medical insurance claimants. Specifically, we evaluated the case-mix index, the numbers of diagnosis and procedure codes utilized, and the corresponding rate of diagnosis codes before, during and after implementation of the DRG payment system. Methods : In order to evaluate the case-mix index, the number of diagnosis and procedure codes utilized, we used medical insurance claim data from all medical facilities that participated in the DRG-based Prospective Payment Demonstration Program. This medical insurance claim data consisted of both pre-demonstration program data (fee-for-service, from November, 1998 to January, 1999) and post-demonstration program data (DRG-based Prospective Payment, from February, 1999 to April, 1999). And in order to evaluate the corresponding rate of diagnosis codes utilized, we reviewed 820 medical records from 20 medical institutes that were selected by random sampling methods. Results : The case-mix index rate decreased after the DRG-based Prospective Payment Demonstration Program was introduced. The average numbers of different claim diagnosis codes used decreased (new DRGs from 2.22 to 1.24, and previous DRGs from 1.69 to 1.21), as did the average number of claim procedure codes used (new DRGs from 3.02 to 2.16, and previous DRGs from 2.97 to 2.43). With respect to the time of participation in the program, the change in number of claim procedure codes was significant, but the change in number of claim diagnosis codes was not. The corresponding rate of claim diagnosis codes increased (from 57.5% to 82.6%), as did the exclusion rate of claim diagnosis codes (from 16.5% to 25.1%). Conclusions : After the implementation of the DRG payment system, the corresponding rate of insurance claim codes and the corresponding exclusion rate of claim diagnosis codes both increased, because the inducement system for entering the codes for claim review was changed.

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A Case Study on the Development and Evaluation of an Web-based Learning Program (웹기반 교육 프로그램의 개발과 프로그램 운영에 따른 효과 고찰)

  • 이영미;장정옥;오유진
    • Journal of Nutrition and Health
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    • v.35 no.8
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    • pp.886-895
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    • 2002
  • Introduction and application of virtual education has been rapidly increased in these days. A variation of information communication technology has an effect on education in interconnect with network as internet in the world that exceed the limit of time and regional. Computer and network communication technology through the medium of internet make an entrance cyber education as a new education paradigm. It must be affective on learner who have various educational characteristics and requirements. It begins to appear quality, quantity improvements of knowledge and the development of information technology that web based cyber education. This study was conducted to develop the web based education program and to evaluate the effectiveness of learning satisfaction and accomplishment and to compare the cyber lecture system with the traditional lecture system During the second semester of 2001, this study was investigated 317 registered students in a "Food and Culture" class at Kyungwon University. The data were obtained from pre and post-study with self-administered questionnaire. The evaluation and satisfaction score of students who were registered in cyberclass was negative tendency to compare pre with post-test scores, because of insuffciency of computer-aided lecture system. The major problem was inconvenient in checking system for connecting times in cyberclass which was one of evaluation point in final score. Another problem was frequently disconnection during cyber studying and not to concentrate each time in the cyber lecture because of eye fatigue, boring due to less interesting contents than other newly developed web-site. The students was prefer to mix type of the cyber and traditional lecture type class. The result of final score an each class, the score of cyber class (71.36 $\pm$ 22.44) was significantly lower than other groups (mixed type : 76.66 $\pm$ 19.99, traditional type :79.17 $\pm$ 15.72) (p < 0.05). Cyber class was attempted to present a useful and interesting teaching and learning tool which can be applied successfully in a longer term. The result suggest that various teaching and learning strategies should be developed considering the fact that the student learn alone most in time.t in time.