• Journal of the Korean Society of Tobacco Science
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• v.16 no.2
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• pp.102-107
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• 1994

This study was conducted to transfer the potato virus Y-necrosis strain resistance from Nicotiana africana to Nicotiana tabacum (cv. NC82) N. africana was crossed with NC82, GErmination of the cotyledon stage, at which time most of the seedling died. However, surviving seedlings continued to grow normally. Chromosomes of the these interspecific self-sterile F1 hybrids were doubled by tissue culture. Amphidiploid of F1 hybrid was self-fertile. Starting with amphidipliod, a wystematic backcross (BC) program was set up with NC82 as recurrent parent. In the BC5C2 generation, the resistant plant was selected. This resistant line, KF8833-1, had 48 chromosome and secreting glandular trichomes. It flowered 2days later than NC82, and stalk height, leaves per plant, leaf length and leaf width were similar to those of NC82.

• Journal of the Korean Society of Tobacco Science
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• v.19 no.2
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• pp.117-123
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• 1997

Viral coat protein (CP) encoding cDNA with artificial start and stop codons was synthesized by reverse-transcriptase polymerase chain reaction (RT-PCR) from the Korean isolate of potato virus Y-vein nectrosis strain (pVY-VN). To make PVY CP cDNA to untranslatable form, three stop codons were inserted near the start codon by "megaprimer-PCR" method. The untranslatable CP cDNA was subcloned to plant expression vector and transferred to N. tabacum cv. NC82 by Agrobacterium-mediated transformation. Highly resistant plants to PVY infection were screened, based on symptom development after mechanical virus inoculation. By genomic PCR and Southern blot analysis, one or more copies of the untranslatable CP gene were found in all transformants. From northern blot analysis, highly resistant transgenic lines had very low level of CP transcript but susceptible lines had high level, suggesting resistance to PVY infection should be related to RNA-mediated mechanism.mechanism.

• Journal of Plant Biotechnology
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• v.36 no.3
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• pp.202-206
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• 2009

In many countries including China and U.S., researchers are developing methods to use sweetpotato as raw material for biofuel. We consider the sweetpotato is not only a source of green fuel, it eventually will provide various material including paper, adhesives, biodegradable plastics, and secondary metabolites. Sweetpotato is one of the high efficiency crop because it yields more calories per unit area than either maize or potato, and it requires the shortest growing cycle of the root crops grown in the tropics. Sweetpotato is the most useful crop for the coming starchbased industry era. Sweetpotato genetic resources are collected, characterized, evaluated, and maintained by U.S., China, Japan, and the International Potato Center. New varieties of sweetpotato using the proper genetic resources and molecular breeding will be developed to cope with the global food and energy in 21st century.

• Korean Journal Plant Pathology
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• v.12 no.1
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• pp.109-115
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• 1996

국내 감자 연작 재배지에서 수집하여 분리동정한 더뎅이병원균인 Streptomyces scabies의 배양적, 형태적, 생리적 특성을 조사한 결과는 다음과 같다. 이병감자에서 분리된 균들은 병원성 균주와 비병원성 균주들로 구분되었고, 이들 간에는 뚜렷한 차이를 보였는데, 전형적인 병징을 나타내는 병원성균주는 비병원성균주와는 달리, 나선형의 포자사슬, 회색 포자, 멜라닌 색소를 생성하고 D-glucose, L-arabinose, D-fructose, D0mannitol, raffinose, rhamnose, sucrose, i-inositol, D-xylose 등의 탄소원을 이용하였으며, 또한 7% NaCl 및 streptomycin sulfate, crystal violet, olean domycin(10$\mu\textrm{g}$/ml, 100$\mu\textrm{g}$/ml)등의 항생물질에 감수성을 나타내었다.

• Journal of Plant Biology
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• v.25 no.2
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• pp.65-72
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• 1982

The oxygen consumption of fresh and aged slices of potato tuber was 40.6 and 168.0 ,$0_2$ $\mu$l/g fr $wt{\cdot}h$, respectively. After initial burst of oxygen consumption within 2 minutes(i.e., 40% for fresh and 12% for aged slices) in the treatmentof $50{\mu}M$ alantolactone, gradual decrease of respiration rate was observed during 3 hours of incubation. In case of the aged slices the initial burst of oxygen consumption was not observed by salicylhydroxamic acid (SHAM) or KCN. The combined effect of SHAM and alantolactone showed increase of 12%, while SHAM and KCN combination showed 60% decrease. In addition, the lipophilicity of cell membrane of onion inner epidermis was increased by lantolactone treatment. The results suggest that the primary effect of alantolactone on the cellular respiration may be associated with an alteration of lipophilic phase of cell membrane and a consequent increase of electron flow throuh the cytochrome system.

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.42-48
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• 2011

Chloroplast genetic engineering of higher plants offers several unique advantages compared with nuclear genome transformation, such as high levels of transgene expression, a lack of position effect due to site-specific transgene integration by homologous recombination, multigene engineering in a single transformation event and reducing risks of gene flow via pollen due to maternal inheritance. We established a reproducible chloroplast transformation system of potato using a tobacco specific plastid transformation vector, pCtVG (trnI-Prrn-aadA-mgfp-TpsbA-trnA). Stable transgene integration into chloroplast genomes and the homoplasmic state of the transgenome were confirmed by PCR and Southern blot analyses. Northern, immunoblot analysis, and GFP fluorescence imaging revealed high expression and accumulation of GFP in the plastids of potato leaves. This system would provide new opportunities for genetic improvement and mass production of value added foreign proteins in this crop.

• The Plant Pathology Journal
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• v.34 no.1
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• pp.23-34
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• 2018

The Ralstonia solanacearum species complex (RSSC) can be divided into four phylotypes, and includes phenotypically diverse bacterial strains that cause bacterial wilt on various host plants. This study used 93 RSSC isolates responsible for potato bacterial wilt in Korea, and investigated their phylogenetic relatedness based on the analysis of phylotype, biovar, and host range. Of the 93 isolates, twenty-two were identified as biovar 2, eight as biovar 3, and sixty-three as biovar 4. Applied to the phylotype scheme, biovar 3 and 4 isolates belonged to phylotype I, and biovar 2 isolates belonged to phylotype IV. This classification was consistent with phylogenetic trees based on 16S rRNA and egl gene sequences, in which biovar 3 and 4 isolates clustered to phylotype I, and biovar 2 isolates clustered to phylotype IV. Korean biovar 2 isolates were distinct from biovar 3 and 4 isolates pathologically as well as genetically - all biovar 2 isolates were nonpathogenic to peppers. Additionally, in host-determining assays, we found uncommon strains among biovar 2 of phylotype IV, which were the tomato-nonpathogenic strains. Since tomatoes are known to be highly susceptible to RSSC, to the best of our knowledge this is the first report of tomato-nonpathogenic potato strains. These results imply the potential prevalence of greater RSSC diversity in terms of host range than would be predicted based on phylogenetic analysis.

• Journal of Bio-Environment Control
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• v.6 no.3
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• pp.198-204
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• 1997

This study was conducted to investigate the effect of solution culture systems on growth and mini-tuber yields of hydroponlcally grown potato (Solanum tuberosun L. cv. Dejima). The growth of stem and foliage at 40 and 60 days after planting were better in aeroponics system than the other systems, and stem length and number of stems per plant at 90 days after planting were the greatest as of 65.4 cm, 7.3 in aeroponics and the lowest as of 49.5 cm, 3.2 in scoria media system. Stolon length was the longest as of 30.4 cm in aeroponics at 90 days after planting, and number of stolons was the greatest as of 10.5 in NFT. Number of mini-tubers per plant at 90 days after planting was 67.1, 62. 5, 20.1 and 18.0 in aeroponics, NFT, perlite and scoria media, respectively. The perlite media system made the fewest enlargement of lenticels of mini-tubers. The results indicate that aeroponic system can be used effectively for mini-tuber potato production.

• Korean journal of applied entomology
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• v.45 no.1 s.142
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• pp.79-85
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• 2006

This study was conducted to get basic information on the occurrence of plant-parasitic nematodes for the establishment of nematode management strategy in major potato production areas in Korea. Nationwide soil collection was done in 11 areas of Cheju, Yesan, Gimchun, Goryoung, Hong chun, Pyungchang, Gimjae, Milyang, Namwon, Gangnung, and Inje in 2004-2005. Root-hot nematode juveniles(J2) were detected in 30 samples among the 50 samples. The average density was 12-69 J2/100cc soil. Pratylenchus sp., Helicotylenchus sp., Ditylenchus sp., Tylenchus sp., and Tylenchorhynchus sp. were also detected in various locations, however, their densities were very low. Root-knot nematode females were collected from tomato roots inoculated with the potato field soils for PCR-RFLP identification. The females from Cheju, Milyang, and Goryung showed PCR products of 500 bp. And the Dra I restriction enzyme digestions showing 290 bp and 230 bp fragments confirmed their identity as Meloidogyne hapla.

• The Plant Pathology Journal
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• v.16 no.2
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• pp.110-117
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• 2000

Potato virus X (PVX-KO) showing mild mosaic and stunting symptoms on potato (Solanum tuberosum) in Kangwon area has been isolated and characterized. EM observation of the purified virus particles showed flexuous rod shape of about 520 nm in length. The coat protein (CP) of the virus had a molecular weight of 31 kDa in SDS-PAGE analysis, and the viral RNA was approximately 6.4 kb in size in denatured agarose gel electro-phoresis. In gel-immunodiffusion tests, it reacted strongly with an antiserum to common PVX from BIOREABAAG (USA). A rabbit antiserum was produced using purified virus and used for routine PVX detection by ELISA. Cultivated potatoes in Kangwon and other areas were frequently infected with PVX-KO. Both Datura stramonium and Nicotiana tabaccum cultivars developed necrotic local lesions 5 days after inoculation, and systemic mosaic symptoms with vein clearing 2 weeks after inoculation. All the features agree with the description of other PVX strains. To confirm and determine PVX strains, reverse transcription-polymerase chain reaction experiment was conducted using specific primers for viral CP. Amplified DNA fragments were cloned and sequenced. Results showed nucleotide sequence homologies of about 88 to 99% to other PVX strains. Based on CP amino acid sequence deduced from nucleotide sequences and host range studies PVX-KO is considered a member of the type X subgroup of PVX.