• Journal of Plant Biology
• /
• v.39 no.2
• /
• pp.93-98
• /
• 1996

In order to enhance the system of potato transformation and further regeneration, potato was transformed using the Agrobacterium tumefaciens harboring $\beta$-glucuronidase (GUS) gene. We found that a series fo modified medium ttained 100% shoot regeneration within 5 weeks after the preincubated explants on stage I medium were infected with Agrobacterium. Callus appeared at the cut edges of stem segments on stage II medium, mainly at the basal parts. Some explants started to form shoots after two to three weeks on stage III medium containing kanamycin (50 mg/L). When transferred to MS medium containing 200 mg/L kanamycin, 81% of the transformed shoots formed roots at the cut edge of the plantlets. In contrast, untrasformed shoots never rooted and became yellowish after few weeks under the same conditions. Southern and northern analysis indicated in vitro shoot regeneration on the callus derived from the potato explants, which were incubated with Agrobacteria. The regeneration cycle was shortened after the transformatin and finally the transformation efficiency was highly enhanced.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.46 no.2
• /
• pp.121-124
• /
• 2001

Potato (Solanum tuberosum L.) tubers of 10, 20, 30, 40, and 50 g were planted in plug trays with vermiculite-based root medium on 10 August 2000 and grown for 15 days in a glasshouse to determine the effects of mini-tuber size on plug seedling growth and field performance of plug seedlings. For a control, common potato tubers weighing 50 g were also planted. As size of seed tubers planted increased from 10 to 50 g, seedling height decreased from 24.6 to 20.0cm while shoot number per seedling increased from 2.0 to 3.5, main stem diameter from 4.3 to 6.1mm, and fresh weight of root + top from 9.3 to 19.4 g/seedling. At 90 days after transplanting plug seedlings, the total number of tubers per plant increased from 3.62 to 4.72, average tuber weight from 62.9 to 72.8g, and total tuber yield 20.5 to 23.6 t/ha with increase in seed tuber size. Plug seedlings raised from 50g tubers produced 22% more tubers per plant and had 21 % higher＞80g tuber yield than the directly planted potatoes.

• Journal of Plant Biotechnology
• /
• v.45 no.3
• /
• pp.207-216
• /
• 2018

Solanum berthaultii is one of the wild diploid Solanum species, which is an excellent resource in potato breeding owing to its resistance to several important pathogens. On the other hand, sexual hybridization between S. berthaultii and S. tuberosum (potato) is limited because of their sexual incompatibility. Therefore, cell fusion can be used to introgress various novel traits from this wild species into the cultivated potatoes. After cell fusion, it is crucial to identify fusion products with the aid of molecular markers. In this study, the chloroplast genome sequence of S. berthaultii obtained by next-generation sequencing technology was described and compared with those of five other Solanum species to develop S. berthaultii specific markers. A total sequence length of the chloroplast genome is 155,533 bp. The structural organization of the chloroplast genome is similar to those of the five other Solanum species. Phylogenic analysis with 25 other Solanaceae species revealed that S. berthaultii is most closely located with S. tuberosum. Additional comparison of the chloroplast genome sequence with those of the five Solanum species revealed 25 SNPs specific to S. berthaultii. Based on these SNPs, six PCR-based markers for differentiating S. berthaultii from other Solanum species were developed. These markers will facilitate the selection of fusion products and accelerate potato breeding using S. berthaultii.

• The Korean Journal of Mycology
• /
• v.35 no.1
• /
• pp.43-46
• /
• 2007

It was investigated that systemic acquired resistance (SAR) was induced in plant treated with a elicitor, which was derived from a non-virulent fungus. The elicitor, a hyphal cell wall component derived from fungus, induced a production of phytoalexin and a generation of reactive oxygen species (ROS) in potato treated with its low level concentrations. The effect of the fungus-derived elicitor was better than that of virulent pathogen-derived elicitor, which was well known in potato. These results, therefore, suggested potentcial use of fungus-derived elicitor as a new plant protector for commercial development.

• Korean Journal Plant Pathology
• /
• v.11 no.1
• /
• pp.73-79
• /
• 1995

Complementary DNAs (cDNAs) to the coat protein gene of an ordinary Korean strain of potato virus Y (PVY-OK) isolated from potato (cv. Superior) were synthesized and cloned into a plasmid pUC119 and sequenced. The RNA of the virus propagated in tobacco (Nicotinaa sylvestris) was extracted by the method of phenol extraction. The first strand of cDNAs to the coat protein penomic RNA of the virus was made by Moloney murine leukemia virus reverse transcriptase. The cDNA were synthesized and amplified by the method of polymerase chain reaction (PCR) using a pair of oligonucleotide primers. PVYCP3P and PVYCP3M. The size of cDNAs inserted in pUC119 plasmid was estimated as about 840 bp upon agarose gel electrophoresis. Double stranded cDNAs were transformed into the competent cell of E. coli JM109. Sequence analysis of cDNAs was conducted by the dideoxynucleotide chain termination method. Homology of cDNAs of the PVY-OK coat protein genomic RNA with those of PVY-O (Japan), PVY-T (Japan), PVY-TH (Japan), PVYN (The Netherlands),and PVYY (France) was represented as 97.3%, 88.9%, 89.3%, 89.6% and 98.5%, respectively. Homology at the amino acid level turned out to the be 97.4%, 92.5%, 92.9%, 92.9% and 98.5%, respectively.

• The Plant Pathology Journal
• /
• v.20 no.2
• /
• pp.110-114
• /
• 2004

Through in vitro screening for biocontrol agents (BCAs) against Streptomyces scabiei causing potato (Solanum tuberosum) common scab, 19 streptomycete and 17 fungal isolates with antagonistic activity were selected as BCA candidates. For the selection of BCA candidates which are highly resistant to 10 kinds of antibiotics or pesticides, chemical susceptibility testing was initially performed in vitro. A remarkable degree of variation in susceptibility to antibiotics or pesticides was observed among the isolates tested. Streptomycete A020645 isolate was highly resistant to all the tested chemicals except neomycin up to 5,000 ppm. On the other hand, out of 36 antagonistic microbes subjected to in vivo pot tests using cultivar Daejima, four streptomycete isolates namely, A020645, A010321, A010564, and A020973, showed high antagonistic activity with >60% and 55% control value, respectively, and high chemical resistance to 10 kinds of chemicals. Therefore, these isolates were selected as potential BCAs for the control of potato common scab.

• Journal of Plant Biotechnology
• /
• v.46 no.2
• /
• pp.79-87
• /
• 2019

One of wild diploid Solanum species, Solanum chacoense, is one of the excellent resources for potato breeding because it is resistant to several important pathogens, but the species is not sexually compatible with potato (S. tuberosum) causing the limitation of sexual hybridization between S. tuberosum and S. chacoense. Therefore, diverse traits regarding resistance from the species can be introgressed into potato via somatic hybridization. After cell fusion, the identification of fusion products is crucial with molecular markers. In this study, S. chacoense specific markers were developed by comparing the chloroplast genome (cpDNA) sequence of S. chacoense obtained by NGS (next-generation sequencing) technology with those of five other Solanum species. A full length of the cpDNA sequence is 155,532 bp and its structure is similar to other Solanum species. Phylogenetic analysis resulted that S. chacoense is most closely located with S. commersonii. Sequence alignment with cpDNA sequences of six other Solanum species identified two InDels and 37 SNPs specific sequences in S. chacoense. Based on these InDels and SNPs regions, four markers for distingushing S. chacoense from other Solanum species were developed. These results obtained in this research could help breeders select breeding lines and facilitate breeding using S. chacoense in potato breeding.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.181-181
• /
• 2017

Global warming and climate change have been one of the most important problems last 2 decades. Global warming is known to cause abnormal climate and influence ecology, food production and human health. According to climate change model global warming is causing expansion of drought and increase of evaporation. Therefore, securing water in agriculture has been an important issue for crop cultivation. As potato is susceptible to drought, water shortage generally results in decrease of yield and decrease of biomass. In this research, we investigated characteristics of inorganic nutrient absorption and growth of plants grown under drought condition. Plants were sampled in sites of Cheong-ju and Gangneung, where the severity of drought stress were different. During the growth period in Gangneung, total rainfall in 2016 decreased by 50% compared with those in last 5 years average. Especially, there was almost no rain in tuber enlargement period (from mid-May to mid-June). On the other hand, the total rainfall in of Cheong-ju was is similar to those in last 5 years average. Inorganic components including K, Ca and Mg and plant growth factors such as plant length, stem length, leaf area index and plant biomass were investigated. Tuber yields in both areas were investigated at harvest. Growth period of plants was is longer in Cheong-ju than that in Gangneung. Contents of all inorganic components were higher in plants grown in Cheong-ju than in Gangneung. The results were attributed to higher production of plant biomass in Cheong-ju. Considering the results, severe drought stress conditions in Gangneung accelerated plant aging and resulted in low plant growth. Although total yield was greatly reduced under drought stress the rate of commercial yield was is not significantly different with non-drought conditions.

• Food Science and Biotechnology
• /
• v.15 no.6
• /
• pp.986-989
• /
• 2006

Sweet potato starch noodles prepared without freezing exhibited higher cooking loss and water uptake during cooking and usually resulted in noodles with a softer and stickier texture compared to commercial sweet potato starch noodles manufactured using a freezing process. By utilizing the starches of different plant sources (potato, cowpea, and sago in an equivalent mixture with sweet potato starch), however, the cooking properties and texture of the starch noodles could be improved. Among the starches tested, cowpea starch was most effective in providing cooking and textural properties similar to those of commercial noodles. As an alternative approach, the addition of a minor amount (0.1 % based on total solid weight) of various gums (xanthan, gellan, locust bean gum, curdlan, and carboxymethyl cellulose) was also examined. The addition of curdlan to noodles was effective in increasing the gumminess and hardness, and reducing the stickiness of noodles. Utilizing different starches and gums can improve the overall texture and quality of sweet potato starch noodles produced without freezing.

• Korean Journal of Breeding Science
• /
• v.42 no.3
• /
• pp.207-211
• /
• 2010

'Taedong Valley' is a high yielding and processing potato cultivar, which is a clonal selection resulting from a cross between 'W870' and 'A88431-1'. It is a medium maturating cultivar with medium plant height and light green foliage. 'Taedong Valley' has profuse flowering habit and light purple flowers. Tubers are smooth, round, and with yellow skin, light yellow flesh, medium eye depth. Tubers have medium dormancy and good keeping quality. 'Taedong Valley' has stable yield under wide range of climatic conditions. It is resistant to common scab and potato virus Y, but moderately susceptible to late blight. It is also resistant to most of the disorders, particularly dehiscence, hollow heart and internal brown spots. This cultivar has high level of tuber uniformity and capable of yielding 43.6 t/ha which is about 9.0% higher than the control potato cultivar 'Atlantic' under optimum agronomical practices.