• The Plant Pathology Journal
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• 제17권6호
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• pp.370.4-370
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• 2001

• The Plant Pathology Journal
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• 제17권6호
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• pp.380.3-380
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• 2001

• 식물병연구
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• 제17권2호
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• pp.129-135
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• 2011

키토산은 D-glucosamine과 N-acetyl-D-glucosamine이 ${\beta}$-(1-4)로 연결된 직선상의 다당류이다. 분자량이 3,000 amu 이상인 키토산에 대한 in vivo 항균활성 및 저항성 유도 활성에 대한 보고는 많지만, 분자량이 3,000 이하인 올리고키토산에 대한 in vivo 항균활성에 대한 논문은 거의 전무한 상태이다. 본 연구에서 올리고키토산은 P. infestans에 의해 발생하는 토마토 역병에 대하여 500 ${\mu}g$/ml과 1,000 ${\mu}g$/ml 수준에서 94% 이상의 매우 높은 접종 1일전 예방효과를 보였으며, 고추 탄저병과 밀 붉은녹병에 대해서는 500 ${\mu}g$/ml과 1,000 ${\mu}g$/ml 수준에서 67% 내지 89%의 중간 정도의 에방효과를 항온항습실 조건에서 보였다. 다른 한편으로 올리고키토산은 고추 탄저병에 대하여 접종 16시간 후 치료효과를 보였지만, 토마토 역병에 대해서는 치료효과를 전혀 보이지 않았다. 포장에서 올리고키토산 액제는 감자 역병과 토마토 역병에 대하여 각각 72%와 48%의 방제효과를 보였다. 이상의 결과는 올리고키토산이 감자 및 토마토 역병 방제를 위한 친환경 유기농자재로서 사용이 가능하다는 것을 강력하게 나타낸다.

• 한국육종학회지
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• 제41권1호
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• pp.61-67
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• 2009

"Early Valley", is an early maturing potato cultivar with high yield potential. "Early Valley" is a clonal selection resulting from the cross between 'Suncrisp' and 'A87109-10'. It has medium plant height and light green foliage. "Early Valley" has medium flowering habit and white flowers. Tubers are smooth, yellow skin, light yellow flesh, round tuber shape, medium eye depth, and medium dormancy and good keeping quality. It has stable yield under wide range of climatic conditions. "Early Valley" is resistance to late blight, but moderately susceptible to common scab and hollow heart. This cultivar is also resistant to potato rotting at harvesting during the raining season. "Early Valley" has high level of antioxidant activity (about three times higher) and vitamin C (higher by 40%) than the 'Superior'. This cultivar has high level of tuber uniformity and capable of yielding 36.56 t/ha which is 17.07% higher than the control potato cultivar 'Superior' under optimum agronomical practices.

• 한국응용곤충학회지
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• 제17권2호
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• pp.81-87
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• 1978

잠자의 중요한 병인 역병(Phytopthora infestans)은 우리나라의 주요씨감자 생산지대인 중부 고랭지대에서 매년 심한 피해를 초래하고 있는데 만일 포장에 역병이 처음 관찰된 후 약제를 살포치 않으면 식물체의 지상부는 14일 이내에 완전고사 하게 된다. 규칙적인 약제살포로 역병방제가 가능하지만 더욱이 역병발생예보에 따라 적기에 약제살포하므로써 방제효과는 물론 생산비를 절감할 수 있게 된다. 본 시험은 1970-1977년 8년간에 걸쳐 대관령의 기상상태와 역병발생 상황을 조사한바 7일 평균기온과 7일 적산강수량을 사용한 Hyre씨의 이동그라프(Moving graph)법에 의한 예보는 정확성이 높지 않았으나 여기에 상대습도와 7일 평균최저기온을 첨가하므로서 비교적 "Moving graph" 법에 의한 예보의 정확성을 높일 수가 있었다. 그러나 아직도 보다 정확한 역병예찰을 위하여 계속적인 조사시험이 필요하다고 생각된다. 또한 약제를 살포하는 곳에서의 역병에 감수성인 품종의 수량은 역병발생정도와 강우량과 밀접한 관계가 있었으며 특히 대관령 지방에서 감자 파종 후 역병발생까지의 일수와 파종 후 90일 수량으로 그 해의 씨감자의 수확양과 공급량을 미리 예측할 수 있게 할 것으로 사료된다.

• 식물병연구
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• 제11권2호
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• pp.122-127
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• 2005

Late blight of potato caused by Phtophthora infestans is one of the most destructive disease in organic farming as well as in the conventional cultivation in Korea. Incidence and progress of the disease largely depend on climatic conditions around the fields. However, the disease completely destroys whole leaves of the plant within two weeks in organic farming fields. While, potato leaves in conventional farming fields remain healthy for 5 weeks after the first symptom appearance, because fungicides were applied four times in average to control the disease. Area under disease progress curve (AUDPC) of organic farming fields ranged from 2314 to 2421 and quickly expanded compare to that of conventional farming fields. The tuber yield in the organic farming fields ranged from 0.96 ton per 10a to 1.5 ton per 10a, while it was 2.7 ton per 10a in conventional farming fields. Tuber yields were reduced to $42\~63\%$ by the infection of late blight and the reduction rate was closely related with the time of first occurrence of the disease in organic farming fields. Physiological races of the pathogen were diversified with R0,1,3,4,5,7,10,11 in organic farming fields at Hongchun area. Pathogen races were distributed with similar pattern at different areas of organic farming fields.

• The Plant Pathology Journal
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• 제23권3호
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• pp.203-209
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• 2007

Infection structures were observed using a fluorescence microscope at the penetration sites on the leaves of potato plants pre-inoculated with the bacterial strains Pseudomonas putida TRL2-3, Micrococcus luteus TRK2-2, and Flexibacteraceae bacterium MRL412, which mediated an induced systemic resistance on potato plants against late blight disease caused by Phytophthora infestans. In order to compare the infection structures on the leaves expressing systemic acquired resistance, the leaves of potato plants pre-treated with DL-3-amino butyric acid (BABA) were also observed after challenge inoculation with the same pathogen. The infection structures were investigated. The total number of germination and appressorium formation of P. infestans were counted. Furthermore, the frequencies of fluorescent epidermal cells at the penetration sites, which indicate a defense response of plant cell, were estimated. There were no differences on the germination rates of the fungal cysts among the untreated control, BABA pre-treated, and bacterial strains pre-inoculated plants. However, appressorium formation was slightly decreased on the leaves of BABA pre-treated plants compared to those of untreated as well as bacterial strains pre-inoculated plants. Furthermore, the frequencies of fluorescent cells of BABA pre-treated and bacterial strains pre-inoculated were higher than that of untreated plants, indicating an active defense reaction of the host cells against the fungal attack. On the other hand, the pre-treatment with BABA caused a stronger fluorescent of epidermal cells at the penetration sites compared to the pre-inoculation with the bacterial strains. Interestingly, the frequency of fluorescent cells by BABA, however, was lower than that by the bacterial strains. Based on the results it is suggested that the infection structures showing resistance reaction on the leaves of potato plants were different between by pre-inoculation with bacterial strains and by pre-treatment with BABA against the late blight pathogen.

• 한국응용곤충학회지
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• 제1권
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• pp.37-41
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• 1962

1. 시험은 1961과 1962년의 2개년에 걸쳐 대관령에서 미국, 일본, 독일계와 한국재래품종 53품종을 공시하여 감자역병에 대한 저항성을 조사할 것이다. 2. 본시험은 자연발병하에서 조사하였으며 항저성의 차이를 다섯 계급으로 구분하였는데 그 결과는 아래와 같다. 면역성품종...Kennebec, Isola, Cherokee, Merrimack, Yoraku, Lisili, Cosima. 고도저항성품종...Antze, Ragis Isola, Delus, Hessenkrone, Plymouth, Sebago, Pungo, Sieglinde, Anco, Essex. 저항성품종...Tawa, B-605-10, Sequoia, Grata, Concordia, Onaway, Noring 1, Nisego. 이병성품종...Nordak, Pontiac, Benimaru, Early gem, Chippewa, Chitose, Ohjiro, Red Bake, Norland, Katahdin. 고도이병성품종...Russet Burbunk, May Queen, Earlaine, Irish Cobbler, Feldeslohn, Red Warba, Chisago, Osseo, Paunee, Warba, Norgleam, Red Beauty, Red, Danshaku, Namchak(Native variety). 3. 만생종품종이 조생종보다 저항성이었다. 4. 일본계와 미국계도입품종보자 독일계도입품종이 저항성이 강한 편이었다.

• The Plant Pathology Journal
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• 제17권6호
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• pp.373.4-373
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• 2001

• 한국균학회소식:학술대회논문집
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• 한국균학회 2015년도 추계학술대회 및 정기총회
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• pp.26-26
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• 2015

Phytophthora infestans is the causal agent of potato and tomato late blight, one of the most devastating plant diseases. P. infestans secretes effector proteins that are both modulators and targets of host plant immunity. Among these are the so-called RXLR effectors that function inside plant cells and are characterized by a conserved motif following the N-terminal signal peptide. In contrast, the effector activity is encoded by the C terminal region that follows the RXLR domain. Recently, I performed in planta functional profiling of different RXLR effector alleles. These genes were amplified from a variety of P. infestans isolates and cloned into a Potato virus X (PVX) vector for transient in planta expression. I assayed for R-gene specific induction of hypersensitive cell death. The findings included the discovery of new effector with avirulence activity towards the Solanum bulbocastanum Rpi-blb2 resistance gene. The Rpi-blb2 encodes a protein with a putative CC-NBS-LRR (a coiled-coil-nucleotide binding site and leucine-rich repeat) motif that confers Phytophthora late blight disease resistance. We examined the components required for Rpi-blb2-mediated resistance to P. infestans in Nicotiana benthamiana. Virus-induced gene silencing was used to repress candidate genes in N. benthamiana and to assay against P. infestans infections. NbSGT1 was required for disease resistance to P. infestans and hypersensitive responses (HRs) triggered by co-expression of AVRblb2 and Rpi-blb2 in N. benthamiana. RAR1 and HSP90 did not affect disease resistance or HRs in Rpi-blb2-transgenic plants. To elucidate the role of salicylic acid (SA) in Rpi-blb2-mediated resistance, we analyzed the response of NahG-transgenic plants following P. infestans infection. The increased susceptibility of Rpi-blb2-transgenic plants in the NahG background correlated with reduced SA and SA glucoside levels. Furthermore, Rpi-blb2-mediated HR cell death was associated with $H_2O_2$, but not SA, accumulation. SA affects basal defense and Rpi-blb2-mediated resistance against P. infestans. These findings provide evidence about the roles of SGT1 and SA signaling in Rpi-blb2-mediated resistance against P. infestans.