• Title/Summary/Keyword: pot type

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Investigation of Siderophore production and Antifungal activity against Phytophthora capsici as related to Iron (III) nutrition by Lysobacter antibioticus HS124

  • Ko, Hyun-Sun;Tindwa, Hamisi;Jin, Rong De;Lee, Yong-Seong;Hong, Seong-Hyun;Hyun, Hae-Nam;Nam, Yi;Kim, Kil-Yong
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.4
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    • pp.650-656
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    • 2011
  • Lysobacter antibioticus HS124 isolated from pepper rhizosphere soil produced catechol type siderophore. Purified siderophore by Diaion HP-20 and silica gel column chromatography showed several hydroxyl functional groups adjacent to benzene rings by analysis of $^1H$ NMR spectroscopy. The strain HS124 showed different activities to suppress Phytophthora capsici with different concentrations of exogenous Fe (III) in minimal medium where antifungal activity with $100{\mu}M$ Fe (III) was approximately 1.5 times higher than in absence of Fe (III). Bacterial population in this Fe (III)-amended medium was also highest with $8.9{\times}10^8\;CFU\;ml^{-1}$ which also corresponded to the strongest siderophore activity. When grown in rich medium (minimal medium with N, $P_2O_5K_2O$ and glucose), HS124 exhibited approximately 2 times stronger antifungal activity compared to minimal medium. In pot trials, treatments of bacterial culture grown in rich medium with (C1) or without (C2) $100{\mu}M$ Fe (III) exhibited a high protection of pepper plants from disease, compared to medium only with (M1) or without (M2) $100{\mu}M$ Fe (III). Especially, treatment C1 showed the best disease control effect of about 70 %. Thus, the strain HS124 should be recommended as a potential biocontrol agent against P. capsici in pepper.

Wind-excited stochastic vibration of long-span bridge considering wind field parameters during typhoon landfall

  • Ge, Yaojun;Zhao, Lin
    • Wind and Structures
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    • v.19 no.4
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    • pp.421-441
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    • 2014
  • With the assistance of typhoon field data at aerial elevation level observed by meteorological satellites and wind velocity and direction records nearby the ground gathered in Guangzhou Weather Station between 1985 and 2001, some key wind field parameters under typhoon climate in Guangzhou region were calibrated based on Monte-Carlo stochastic algorithm and Meng's typhoon numerical model. By using Peak Over Threshold method (POT) and Generalized Pareto Distribution (GPD), Wind field characteristics during typhoons for various return periods in several typical engineering fields were predicted, showing that some distribution rules in relation to gradient height of atmosphere boundary layer, power-law component of wind profile, gust factor and extreme wind velocity at 1-3s time interval are obviously different from corresponding items in Chinese wind load Codes. In order to evaluate the influence of typhoon field parameters on long-span flexible bridges, 1:100 reduced-scale wind field of type B terrain was reillustrated under typhoon and normal conditions utilizing passive turbulence generators in TJ-3 wind tunnel, and wind-induced performance tests of aero-elastic model of long-span Guangzhou Xinguang arch bridge were carried out as well. Furthermore, aerodynamic admittance function about lattice cross section in mid-span arch lib under the condition of higher turbulence intensity of typhoon field was identified via using high-frequency force-measured balance. Based on identified aerodynamic admittance expressions, Wind-induced stochastic vibration of Xinguang arch bridge under typhoon and normal climates was calculated and compared, considering structural geometrical non-linearity, stochastic wind attack angle effects, etc. Thus, the aerodynamic response characteristics under typhoon and normal conditions can be illustrated and checked, which are of satisfactory response results for different oncoming wind velocities with resemblance to those wind tunnel testing data under the two types of climate modes.

Siderophore-producing rhizobacteria reduce heavy metal-induced oxidative stress in Panax ginseng Meyer

  • Huo, Yue;Kang, Jong Pyo;Ahn, Jong Chan;Kim, Yeon Ju;Piao, Chun Hong;Yang, Dong Uk;Yang, Deok Chun
    • Journal of Ginseng Research
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    • v.45 no.2
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    • pp.218-227
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    • 2021
  • Background: Panax ginseng is one of the most important medicinal plants and is usually harvested after 5 to 6 years of cultivation in Korea. Heavy metal (HM) exposure is a type of abiotic stress that can induce oxidative stress and decrease the quality of the ginseng crop. Siderophore-producing rhizobacteria (SPR) may be capable of bioremediating HM contamination. Methods: Several isolates from ginseng rhizosphere were evaluated by in vitro screening of their plant growth-promoting traits and HM resistance. Subsequently, in planta (pot tests) and in vitro (medium tests) were designed to investigate the SPR ability to reduce oxidative stress and enhance HM resistance in P. ginseng inoculated with the SPR candidate. Results: In vitro tests revealed that the siderophore-producing Mesorhizobium panacihumi DCY119T had higher HM resistance than the other tested isolates and was selected as the SPR candidate. In the planta experiments, 2-year-old ginseng seedlings exposed to 25 mL (500 mM) Fe solution had lower biomass and higher reactive oxygen species level than control seedlings. In contrast, seedlings treated with 108 CFU/mL DCY119T for 10 minutes had higher biomass and higher levels of antioxidant genes and nonenzymatic antioxidant chemicals than untreated seedlings. When Fe concentration in the medium was increased, DCY119T can produce siderophores and scavenge reactive oxygen species to reduce Fe toxicity in addition to providing indole-3-acetic acid to promote seedling growth, thereby conferring inoculated ginseng with HM resistance. Conclusions: It was confirmed that SPR DCY119T can potentially be used for bioremediation of HM contamination.

Positive Effects of Humic Substances on Plant Growth and Biological Soil Indicators when Spring barley is Green Manured on Reclaimed Soils (처리토에 녹비 식물 청보리 경작 시 휴믹 물질이 식물생장 및 생물학적 토양 인자에 주는 긍정적 영향)

  • Sua Kang;Hyesun Park;Younrho Lee;Bumhan Bae
    • Journal of Soil and Groundwater Environment
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    • v.29 no.1
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    • pp.51-62
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    • 2024
  • A study was performed to investigate the positive impacts of humic substances (HS) on the growth of green barley, a type of green manure plant. The study was conducted in a pot culture using two different types of reclaimed soils that had been treated by land farming (DDC) and thermal desorption (YJ) methods, respectively. The experimental conditions consisted of three treatments: plant only (P), plant plus 2% HS, and no plant (control). After 89 days of culture in a controlled growth chamber, the growth of spring barley and activity of seven soil enzymes were measured. The results indicated that the addition of HS had a substantial (p<0.10) positive effect on shoot biomass in both types of soil. Furthermore, the addition of HS notably (p<0.05) enhanced all seven soil enzyme activities in both soils. Both the aboveground and belowground parts of barley plants were returned to soil and aged for 10 weeks in the same growth chamber, which resulted in notable enhancement in soil health indicators. These improvements included an increase in organic matter, a drop in bulk density, and an increase in the activity of seven different soil enzymes. When lentil seeds were planted in the aged soils, the development of the seedlings was more vigorous than that in the control in both soils, although allelopathy of barley suppressed lentil germination in soil with pH 7.0 but not in soil with pH 8.5.

Development of Light Distributed Solar Modules for Growing Sprout Ginseng and Overseas Empirical Research on Natural Lighting Cultivation System (새싹인삼 재배를 위한 투광 분산형 태양광 모듈 개발 및 자연채광 재배시스템 해외 실증연구)

  • Seong-Hwan Kang;Jae-ho Choi;In-Sung Jung;Gyeong-Yeol Lim;Jae-Woo Park;Nguyen Huu Hoang;Chong-Yeal Kim
    • Current Photovoltaic Research
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    • v.12 no.1
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    • pp.24-30
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    • 2024
  • To create an environment for growing ginseng sprouts, we developed a solar module that partially transmits and disperses light. A G to G type light transmission and dispersion solar module was developed using glass with a mist pattern applied, and the light dispersion effect of the developed module was confirmed through illuminance measurement. The output of one module is approximately 260 W, and the configuration consists of 48 cells in series in 4 strings. The cultivation system where the developed module will be installed was developed in the form of a container, and three units of 2.6 kW (260 W x 10 EA). The inside of the cultivation system consists of a shading screen, air conditioner, ventilator, plastic pot, etc. to create an environment for cultivating sprout ginseng. As a result of actually planting sprout ginseng, it was confirmed through verification that the plants were grown without any problems.

Desulfurization Reaction according to Ladle Slag Recycling Method in Shaft-Type EAF Operation (Shaft형 전기로 공정에서 ladle 슬래그 재활용 방법에 따른 탈황반응)

  • Jung-Min Yoo
    • Resources Recycling
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    • v.33 no.2
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    • pp.46-53
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    • 2024
  • The residual heat and high CaO content present in the slag remaining in the ladle after the completion of continuous casting in the electric arc furnace (EAF) steelmaking process have been utilized to reduce power consumption and lime usage in the ladle furnace (LF) process. However, if the timing of such processes does not align with the LF and continuous casting operations, the recycling rate will decrease. To increase the slag recycling rate, the effect of ladle slag recycling methods, specifically pouring ladle slag into the slag pot in advance for subsequent recycling, on LF operations was analyzed. The slag liquefaction rate was calculated using the thermodynamic program Factsage 8.3 for ladle molten slag recycling methods. By applying each of the 10 heats operations for the ladle slag recycling methods, the desulfurization ability and LF operation performance were compared. It was found that when slag was immediately recycled into the ladle after continuous casting was completed, power consumption decreased by 0.3 MWh, LF operation time was shortened by 1.2 minutes, and the desulfurization rate increased by 5.8%.

Studies on the Low Temperature Injury of the Rice Varieties (통일계(統一系) 수도품종(水稻品種)의 저온장해(低溫障害)에 관(關)한 연구(硏究))

  • Choi, Chang-Yoel;Kim, Moon-Kyu;Jo, Jai-Seong;Kim, Choong-Soo
    • Korean Journal of Agricultural Science
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    • v.4 no.1
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    • pp.10-20
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    • 1977
  • An expriment was conducted to determine the rate of germination under low temperature and effects of low temperature on seedling of varieties derived from the cross between Indica and Japonica type of rice. Total of 30 varieties including leading variety, Tongil, were germinated at the temperature of $10^{\circ}C$. and $15^{\circ}C$. to determine the rate of germination, and six varieties also including Tongil were planted in pot, and seedlings were exposed to the temperature of $5^{\circ}C$. and $1^{\circ}C$. for 15 days and damage of plant growth due to low temperature was observed. 1. Under the condition of $10^{\circ}C$., varieties such as Iri #323, Suwon #253, Iri #325, Milyang #22, Suwon #251, and Suwon #267 were showing over 70 percent of germination ratio, while varieties, Dodolokiwase, Milyang #23, Yusin, Iri #328, and Iri #329 were below 10 percent in germination ratio. 2. Under the condition of $15^{\circ}C$., variety Suwon #262 was shown 100 percent of germination and varieties such as Milyg an#22, Dodlokiwase, Tongil, Milyang #29, Suwon #258, Milyang #23, Milyang #24, Milyang #28 and Milyang #21 were over 90 percent in germination ratio. However, varieties such as Iri #328, Iri #329, Jinheung, and Minehikari were below 50 percent in germination rate. 3. Considering the germination rate and average days required to germination, Milyang #22 and Iri #323 were highly resistant varieties to low temperature. The rate of germination of varieties from the cross between Indica and Japonica under low temperature was not always lower than that of varieties from Japonica type of rice. 4. Most of the seedlings of varieties used were quite resistant to $1^{\circ}C$. at least for six hours. However, leaves of rice were exposed to the the temperature of $1^{\circ}C$. for 12 hours, and the withered leaves were recovered soon when put in under normal temperature. The degree of leaf withering under low temperature was lower in Milyang #15, Japonica type than in varietieties belonging to $Indica{\times}Japonica$ type of rice. 5. When the seedlings of varieties such as Josaengtongil, Tongil and Yusin were exposed to the temperature of $1^{\circ}C$. for 36 hours, 70 percent of leaves were withered and when the time of expose were doubled, the leaves were completely died. When the temperature was fluctuated, over 75 percent of leaves were died, while 65 percent of leaves of Milyang #15 were died when exposed to $1^{\circ}C$, for 72 hours. 6. Significant growth retardation was observed for all entries when exposed to $1^{\circ}C$. for 24 to 36 hours. The growth retardation was apparently increased as the time of expose was extended.

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Ideal Leaf Type on Leaf Shape and $\textrm{CO}_2$ Use Efficiency of Different Seed Size Cultivar in Soybean (엽형 및 $\textrm{CO}_2$ 이용효율에 따른 콩 입중별 이상초형 연구)

  • 이강세;전병무;김영진;국용인;박호기;박문수
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.3
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    • pp.243-247
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    • 2003
  • To examine ideal leaf types with higher $\textrm{CO}_2$ assimilates and different seed sizes, 12 soybean varieties were planted on the pot in a plastic house covered with glass. Leaf function based on stomatal conductance and $\textrm{CO}_2$ assimilation in soybean is different in seed size and leaflet shape. Mean $\textrm{CO}_2$ assimilation of a single leaf was 19.66 $\mu\textrm{molm}^{-2}\textrm{s}^{-1}$ and showed higher in small seed cultivars with narrow leaflet than that of small seeds with wide leaflet (18.29 $\mu\textrm{molm}^{-2}\textrm{s}^{-1}$), but within large seed groups, it was higher in wide leaflets (19.17 $\mu\textrm{molm}^{-2}\textrm{s}^{-1}$) than narrow leaflet cultivars (17.45 $\mu\textrm{molm}^{-2}\textrm{s}^{-1}$). In small seed and narrow leaflet cultivars, stomatal conductance ranged from 0.14 to 0.15 $\mu\textrm{molm}^{-2}\textrm{s}^{-1}$, while $\textrm{CO}_2$ assimilation ranged from 19 to 20 $\mu\textrm{molm}^{-2}\textrm{s}^{-1}$. The Photosynthetic rate was closely related to stomatal conductance, transpiration and water use efficiency.

The Effects of Soybean Boiling Waste Liquor on the Enhancement of Lactic Acid Fermentation during Korean Traditional kanjang Mash Maturing (한국 재래식 간장덧 발효시 대두 자숙 폐액 첨가가 젖산발효 촉진에 미치는 영향)

  • Choi, Cheong;Im, Moo-Hyeog;Choi, Jong-Dong;Chung, Hyun-Chae;Kim, Young-Ho;Lee, Choon-Woo;Choi, Kwang-Soo
    • Applied Biological Chemistry
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    • v.41 no.3
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    • pp.201-207
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    • 1998
  • In order to evaluate the effects of addition of soybean boiling waste liquor (SBWL) and sugar and inoculation of the lactic acid bacteria and yeast starter culture in Korean traditional kanjang mash, three types of kanjang were prepared in a clay pot of 100 l volume and compared the characteristics of lactic acid fermentation. The mashing compositions of the types of kanjang were as follows: (1) control treatment mash was prepared with meju : 20% salt solution (1:4) and SBWL, (2) kanjang mash with 3.5% added sugar to the control type mash and (3) kanjang mash with 3.5% added sugar and inoculation of the lactic acid bacteria and yeast starter culture 35 days after mashing to the control type mash. (1), (2) and (3) of kanjang mash were found to be effective in increasing the lactic acid content and improving the organoleptic characteristics of kanjang. But the effect of yeast starter culture was not clear because osmophilic yeasts were inhibited by metabolite(acetic acid) produced by lactic acid bacteria. The lactic acid content of (1), (2) and (3) kanjang was 2.05, 2.38 and 2.91% respectively in 90 day-matured kanjang.

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Physiological responses to drought stress of transgenic Chinese cabbage expressing Arabidopsis H+-pyrophosphatase (애기장대 H+-pyrophosphatase 발현 형질전환 배추의 건조스트레스에 대한 생리적 반응)

  • Jeong, Mihye;Kang, In-Kyu;Kim, Chang Kil;Park, Kyung Il;Choi, Cheol;Han, Jeung-Sul
    • Journal of Plant Biotechnology
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    • v.40 no.3
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    • pp.156-162
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    • 2013
  • Plant tolerance to drought is a beneficial trait for stabilizing crop productivity under water deficits. Here we report that genetically engineered Chinese cabbage expressing Arabidopsis $H^+$-pyrophosphatase (AVP1) shows enhanced physiological parameters related to drought tolerance. In comparison with wild type plants under soil water deficit stress created by cessation of irrigation, soil water potential in pot with AVP1-expressing plants was more rapidly decreased that might lead to increased relative water content in leaves, while both genotypes had indistinguishable wilting phenotypes. Transgenic plants subjected to drought treatment also exhibited higher photosystem II quantum yield in addition to lower electrolyte leakage and $H_2O_2-3,3^{\prime}$-diaminobenzidine content when compared to wild type plants.