• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.2 no.2
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• pp.114-119
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• 2021

To restore the Gold-spotted pond frog (Pelophylax chosenicus), a Korean endangered frog, 600 captive-reared individuals were reintroduced between August and September 2019 into an aquatic garden in the National Institute of Ecology where P. chosenicus had previously inhabited. After reintroduction, six post-release monitoring sessions were conducted from August 2019 to May 2020. Monitoring was performed using three methods (counting calls, observation, and capture) from sunset to midnight near release sites, eliminating potential threat factors. Snout-vent lengths and body weights of recaptured individuals were measured before they were released immediately. We noted that both snout-vent lengths and body weights of recaptured individuals were slightly higher than those before. The average recapture rate was 4.66%. Reintroduced frogs were recaptured at the last monitoring session conducted in May 2020, indicating that these reintroduced frogs hibernated during the winter successfully. We found that these reintroduced frogs successfully settled after release. This result will be useful for establishing management strategies for endangered frogs in Korea. Particularly, post-release monitoring could be an essential approach in the restoration program of a target species.

• Journal of Veterinary Science
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• v.24 no.1
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• pp.17.1-17.9
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• 2023

To our knowledge, this is the first case of concurrent diaphyseal left coracoid and left femoral fractures in a Eurasian eagle owl and its post-release survival in Korea. The femur was surgically repaired using an external skeletal fixator-intramedullary (IM) pin tie-in method, and the coracoid was repaired solely with an IM pin on day 6 after femur surgery. The eagle owl underwent a gradual rehabilitation process. The bird was successfully rehabilitated and released 101 d after initial presentation. The bird was monitored using a wildlife tracking device and was confirmed to have survived for over 5 mon in the wild.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.2 no.4
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• pp.247-258
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• 2021

To understand restoration and conservation projects conducted in Korea for endangered freshwater fishes and amphibians/reptiles, information about Request for Protocols-related studies on restoration, breeding, and release were collected. Trends of studies were visualized via word clouds and VOSviewer program using a text mining technique. Analysis of restoration projects for endangered freshwater fishes elucidated that most research studies conducted to date were focused on genetics and release through captive breeding that could be classified into captive breeding and habitat environments. As for research projects related to amphibians/reptiles, monitoring projects had the highest number, followed by genetic, translocation, and monitoring studies. In addition, restoration projects for amphibians/reptiles included a large number of post-capture translocation projects. Thus, many projects were confirmed by public institutions rather than by the Ministry of Environment. Network analysis revealed that it was largely classified into capture, translocation, and Kaloula borealis. Based on these results, limitations, achievements, and challenges associated with projects conducted thus far are highlighted. Research directions for future restoration and conservation of endangered freshwater fishes and amphibians/reptiles in South Korea are also suggested.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.6
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• pp.85-95
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• 2015

Recent years, nationwide projects for ecological restoration are implemented with emerging issues on the stream ecosystem. In order to enhance effectiveness of the ecosystem restoration and reduce negative impact, the appraisal of effectiveness through the follow-up monitoring and the adaptive management process are executed in consecutive phase. In this study, planning phase, monitoring and adaptive management in Hakui stream which is part of An Yang stream restoration project is introduced as representative ongoing case of effective adaptive management. The aim of this study is to verify the adaptive management process and suggest direction of effective restoration. Restoration project of Hakui stream resulted in increasing number and diversity of species (vegetation, fish, bird, invertbrates, amphibian and reptilia) according to monitoring from 2004 to 2013, and enhancing natural river landscape by evaluation of river naturalness among 2001(before restoration), 2007 (after), 2015 (recent). However, excessive vegetation expansion or sediment deposition on channel over time caused unexpected results such as terrestrialization or degradation of habitats. Adaptive management action such as removing disturbance species (Humulus japonicus)(2007), coppicing willow (2007), release of march snail (2007), creation of wetland (2014) were implemented based on monitoring results. And then appraisal of management action was discussed.

• Mass Spectrometry Letters
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• v.12 no.3
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• pp.85-92
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• 2021

The therapeutic antibody drug market has experienced explosive growth as mAbs become the main therapeutic modality for a variety of diseases. Characterization of glycosylation that directly affects the efficacy and safety of therapeutic monoclonal antibodies (mAbs) is critical for therapeutics development, bioprocess system optimization, lot release, and comparability evaluation. The LC/MS approach has been widely used to structurally characterize mAbs, and recently attempts have been made to obtain comprehensive information on the primary structure and post-translational modifications (PTMs) of mAbs through intact protein analysis. In this study, we performed state-of-the-art LC/MS based intact protein analysis to readily identify and characterize glycoforms of various mAbs. Different glycoforms of mAbs produced in different expression cell lines including CHO, SP2/0 and HEK cells were monitored and compared. In addition, the comparability of protein molecular weight, glycoform pattern, and relative abundances of glycoforms between the commercialized trastuzumab biosimilar and the original product was determined in detail using the given platform. Intact mAb analysis allowed us to gain insight into the overall mAb structure, including the complexity and diversity of glycosylation. Furthermore, our analytical platform with high reproducibility is expected to be widely used for biopharmaceutical characterization required at all stages of drug development and manufacturing.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.04a
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• pp.3-16
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• 2000

The ultimate safety-goal of nuclear power plants should be targeted at preventing release of nuclear radiation compared to general structures, Accordingly the phases of siting design construction and operation of NPPs are severely regulated by codes of aseismic design so as to assure safety of NPPs. To accomplish this goal strict quality assurace and seismic qualification tests should be conducted for all phases of NPP construction. In addition seismic monitoring systems should be installed and always in operation to provide proper post-earhquake procedures. Besides periodic safety review should be performed during operation along with the seismic margin assessment. In this paper general procedures to secure seismic safety of NPPs are systematically reviewed and additional considerations for improvement are suggested.

• Structural Engineering and Mechanics
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• v.89 no.6
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• pp.617-626
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• 2024

The study investigated the behavior of plain and fibered Ultra-High Performance Concrete (UHPC) beams under varying loading conditions using integrated analysis of the flexure and acoustic emission tests. The loading rate of testing is -0.25 -2 mm/min. It is observed that on increasing loading rate, flexural strength increases, and toughness decreases. The acoustic emission testing revealed that higher loading rates accelerate crack propagation. Fiber effect and matrix cracking are identified as significant contributors to the release of acoustic emission energy, with fiber rupture/failure and matrix cracking showing rate-dependent behavior. Crack classification analysis indicated that the rise angle (RA) value decreased under quasi-static loading. The average frequency (AF) value increased with the loading rate, but this trend reversed under rate-dependent conditions. K-means analysis identified distinct clusters of crack types with unique frequency and duration characteristics at different loading rates. Furthermore, the historic index and signal strength decreased with increasing loading rate after peak capacity, while the severity index increased in the post-peak zone, indicating more severe damage. The sudden rise in the historic index and cumulative signal strength indicates the possibility of several occurrences, such as the emergence of a significant crack, shifts in cracking modes, abrupt failure, or notable fiber debonding/pull-out. Moreover, there is a distinct rise in the number of AE knees corresponding to the increase in loading rate. The crack mapping from acoustic emission testing aligned with observed failure patterns, validating its use in structural health monitoring.