• Title/Summary/Keyword: post-larvae

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Characterization of a novel Cotesia vestalis polydnavirus (CvBV) gene containing a ser-rich motif expressed in Plutella xylostella larvae

  • Shi, Min;Chen, Ya-Feng;Huang, Fang;Zhou, Xue-Ping;Chen, Xue-Xin
    • BMB Reports
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    • v.41 no.8
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    • pp.587-592
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    • 2008
  • Cotesia vestalis is an endoparasitoid of Plutella xylostella larvae and injects a polydnavirus (CvBV) into its host during oviposition. In this report we characterize the gene, CvBV3307, and its products. CvBV3307 is located on segment S33 of the CvBV genome, is 517 bp, and encodes a putative protein of 122 amino acids, including a serine-rich region. The expression pattern of CvBV3307 in parasitized larvae and the subcellular localization of CvBV3307 only in granulocytes indicated that it might be involved in early protection of parasitoid eggs from host cellular encapsulation and in manipulating the hormone titer and developmental rhythm of host larvae. Western blot analysis showed that the size of the immunoreactive protein (about 55 kDa) in parasitized hosts at 48 hours post parasitization (h p.p.) is much larger than the predicted molecular weight of 13.6 kDa, which suggests that CvBV3307 undergoes extensive post-translational modification in hosts.

Effect of Testosterone Propionate on the Economic Traits of the Silkworm, Bombyx mori L.

  • Magadum, V.B.;Magadum, S.B.
    • Journal of Sericultural and Entomological Science
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    • v.35 no.1
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    • pp.69-72
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    • 1993
  • Effects of topical application with 1, 5 and 10 ${\mu}$g/ml testosterone on the precocooning and post cocooning traits of the polyvoltine. Pure Mysore breed of silkworm were studied. Each concentration was treated independently at 36th to III, IV and V instar larvae(Treatment-1), at 48th to IV and V instar larvae(Treatment-2_ and at 72th to V instar larvae(Treatment-3). The repeated application with each concentration of testosterone propionate in treatment, and 2 larvae or single application in treatment 3 variously affected the precocooning and post cocooning traits. Repeated applications of all doses of testosterone propionate(Treatment-1) and single application of 1 & 5 ${\mu}$g of the chemical(Treatment-3) increase the fecundity and decrease the percentage of cocooning and moth emergence.

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Identification of highly pathogenic Beauveria bassiana strain against Pieris rapae larvae

  • DING, Jun-nan;LAI, Yong-cai
    • Entomological Research
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    • v.48 no.5
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    • pp.339-347
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    • 2018
  • Seven different strains of Beauveria bassiana were used in a bioassay on Pieris rapae larvae. The results showed that an B. bassiana strain showed relatively high pathogenicity towards P. rapae larvae. The adjusted mortality rate was 92.86 %, and the infection rate was 85.71 % in 10 days post inoculation. Molecular identification was performed to identify the unknown strain. Internal Transcribed Spacer sequence analysis showed that the polymerase chain reaction amplicon length of the unknown strain of Beauveria sp. was 573 bp, and sequence similarity to the known B. bassiana sequences in the NCBI database was 99 %. The B. bassiana strain was named Bb01. The changes of proteins and PPO of P. rapae larvae infected by B. bassiana Bb01 strain at different times was determined. The activity of PPO increased in 1-6 d and decreased in 7 d again after inoculation. The B. bassiana invaded into the insect body affected the balance of the proteins and PPO.

The Larvae and Juvenile Development of Haddock, Melanogrammus aeglefinus Cultured in Atlantic Canada (Atlantic Canada 해산어 Haddock, Melanogrammus aeglefinus의 자치어 발생 단계)

  • Kim Chi-Hong;Im Jae Hyun;Johnson Stewart C;Hur Jun Wook;Park In-Seok
    • Development and Reproduction
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    • v.8 no.1
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    • pp.11-17
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    • 2004
  • The larvae and juvenile development of haddock Melanogrammus aeglefinus which is significant commercial fish living north Atlantic Ocean are described here. Larvae were reared in laboratory and sampled periodically for developmental study until 67 days after hatching. An increase in total length(TL) of fish indicated continuous growth, described by the growth expression Y=4.07 $e^{0.037}$( $R^{2}$=0.9978). The newly hatched pre-larvae was 4.9 mm in TL with ellipsoid yolk. In 16 days after hatching, larvae attained 6.8 mm in TL, and absorbed the yolk completely to become post-larval stage, but first heterotrophic food could be in 7 days after hatching already. Post-larval stage continued during 16~52 days after hatching with development of organs attachment. In 61 days after hatching with 41.3 mm in TL, the fries became a juvenile stage respectively having small teed lateral line, and a black blotch on the flank same as adults, but chin barbel was not developed yet. It was presumed that haddock changed food and ecological behavior after metamorphosis ken this time.e.

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Longevity of Toxocara cati Larvae and Pathology in Tissues of Experimentally Infected Chickens

  • Oryan, Ahmad;Sadjjadi, Seyyed-Mahmoud;Azizi, Shahrzad
    • Parasites, Hosts and Diseases
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    • v.48 no.1
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    • pp.79-80
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    • 2010
  • This study was conducted to determine the distribution patterns and duration of stay of Toxocara cati larvae in organs of chickens and to investigate chronic phase and potential zoonotic risk of toxocariasis in chickens. Chickens were orally infected with 1,000 embryonated T. cati eggs and necropsied 240 days post-infection. Organs of the chickens were examined at gross and microscopic levels; tissues were digested to recover larvae. Peribronchiolitis with infiltration of lymphocytes, and hyperplasia of bronchiolar associated lymphatic tissues (BALT) and goblet cells, were evident in the lungs of infected chickens. There were mild hemorrhages and infiltration of lymphocytes and a few eosinophils in the meninges. Larvae were recovered from 30% of the exposed chickens. Larvae recovery indicated that T. cati larvae stay alive for at least 240 days in the chicken brain. Therefore, chickens may potentially act as a paratenic host in nature and transfer T. cati larvae to other hosts.

Artificial Spawning Behavior and Development of Eggs, Larvae and Juveniles of the Red Spotted Grouper, Epinephelus akaara in Korea

  • Park, Jong Youn;Cho, Jae Kwon;Son, Maeng Hyun;Kim, Kyong Min;Han, Kyeong Ho;Park, Jae Min
    • Development and Reproduction
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    • v.20 no.1
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    • pp.31-40
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    • 2016
  • This study was conducted in order to examine the egg development in red spotted grouper, Epinephelus akaara and the morphological development of its larvae and juveniles, and to obtain data for taxonomic research. This study was conducted in June 2013, and 50 male and female fish were used for the study. One hundred ${\mu}g/kg$ of LHRHa was injected into the body of the fish for inducing spawning, and the fish were kept in a small-sized fish holder ($2{\times}2{\times}2m$). Eggs were colorless transparent free pelagic eggs, 0.71-0.77 mm large (mean $0.74{\pm}0.02mm$, n=30), and had an oil globule. Hatching started within 27 h after fertilization. Pre-larvae that emerged just after hatching were 2.02-2.17 mm in total length (mean $2.10{\pm}0.11mm$), their mouth and anus were not opened yet, and the whole body was covered with a membrane fin. Post-larvae that emerged 15 days post hatching were 3.88-4.07 mm in total length (mean $3.98{\pm}0.13mm$), and had a ventral fin with two rays and a caudal fin with eight rays. Juveniles that were formed at 55 d post hatching, were 31.9-35.2 mm in total length (mean $33.6{\pm}2.33mm$), with red color deposited over the entire body, and black chromophores deposited in a spotted pattern. The number of fin rays, body color, and shape were the same as that in the adult fish.

ON THE EFFECTS CHLORINITIES UPON GROWTH OF EARLIER LARVAE AND POST-LARVA OF A FRESH WATER PRAWN, MACROBRACHIUM ROSENBERGI(DE MAN) (담수산새우 Macrobrachium rosenbergi (de Man)의 초기유생 및 Post-larva.의 성장에 미치는 염분량에 관하여)

  • KWON Chin Soo;UNO Yutaka;OGASAWARA Yohismitsu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.10 no.2
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    • pp.97-114
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    • 1977
  • The fresh water prawn, Macrobrachium rosenbergi(de Man) is a very common species in Indopacific region, which inhaits both fresh and brackish water in low land area, most of rivers and especially aboundant in the lower reaches which are influenced by sea water. It is one of the largest and commercial species of genus Macrobrachium, which is commonly larger than $18\~21cm$ in body length, from the basis of eye-stalked to the distal of telson. As a part of the researches in order to investigate the possibilities on transplantation and propagation of this species, this work dealt with the problems on the effects of chlorinities upon zoeal larvae and post-larvae 1). metamorphosis rate and optimum chlorinity for metamorphosis to post-larve, 2). tolerance and comparative survival rate on various chlorinties, from fresh water to sea water $(19.38\%_{\circ}\;Cl)$, which reared for six days upon each stage of zoeal larvae, 3). accomodation rate on chlonities which reared for twelve days after transmigration into variant chlorinities of the range from $3.68\%_{\circ}$ Cl to $1.53\%_{\circ}$ Cl in the way of rearing of the range from $3.82\%_{\circ}$ Cl to $11.05\%_{\circ}$ upon each stage of zoea, 4). tolerance on both of fresh and sea water upon zoeal larva and post-larva under the condition of $28^{\circ}C{\pm}1$ in temperature and feeding on Artenia salina nauplii, 5). relationship between various chlorinities and grwth of post-larvae under the condition of $28^{\circ}C$ in tmperature and feeding on meat of clam. Thus these investigations were performed in order to grope for a comfortable method on seedmass production. Up to the present, the study on the effects of chlorinity upon earlier zoeal larvae and post-larvae of Macrobrachium species has been scarcely performed by workers with the exception of Lewis(1961) and Ling (1962,, 1967), even so their works were not so detailed. On the other hand, larvae of several species of this genus were reared at the water which mixed sea water so as to carry out complete metamorphosis to post-larva by workers in order to investigate on earlier 1 arval and earlier post-larval development, such as Macrobrachium lamerrei (Rajyalakshmi, 1961), M. rosenbergi and M. nipponense (Uno and Kwoa, 1969; Kwon and Uno, 1969), M. acanthurs (Choudhury, 1970; Dobkin, 1971), M. carcinus(Choudhury, 1970), M. formosense(Shokita, 1970), M. olfersii (Duggei et al., 1975), M. novaehallandiae (Greenwood et al., 1976), M. japonicum (Kwon, 1974) and M. lar (Shokita, personal communication), and there fore it is regarded that chlorinity is, generally, one of absolute factors to rear zoeal larvae of brackish species of Macrobrachium genus. Synthetic results on this work is summarized as the follwings: 1) Zoeal larvae required different chlorinities to grow according to each stage, and generally, it is regarded that optimum range of living and growing is from $7.63\%_{\circ}Cl\to\;7.63\%_{\circ}Cl$, and while differences of metamorphsis rate, from first zoea to post-larva, is rarely found in this range, and however it occurs apparently in both of situation at $7.63\%_{\circ}Cl$ below and $16.63\%_{\circ}Cl$ above and moreover, metamorphosis rate is delayed somewhat in case of lower chlorinity as compared with high chlorinity in these situations. 2) Accomodation in each chlorinity on the range, from fresh water to sea water, is different according to larval stages and while the best of it is, generally, on the range from $14.24\%_{\circ}Cl$ to $8.28\%_{\circ}Cl$ and favorite chlorinity of zoea have a tendency to remove from high chlorinity to lower chlorinity in order to advance larval age throughout all zoeal stages, setting a conversional stage for eighta zoea stage. 3) Optimum chlorinity of living and growth upon postlarvae is on the range of $4.25\%_{\circ}Cl$ below, and in proportion as approach to fresh water, growth rate is increased. 4) Post-large are able to live better in fresh water in comparison with zoeal larvae, which are only able to live within fifteen hours, and by contraries, post-larvae are merely able to live for one day as compared with ?미 larvar, which are able to live for six days more in sea water $19.38\%_{\circ}Cl\;above$. 5) Also, in case of transmigration into higher and lower chlorinities in the way of rearing in the initial chlorinities $ 3.82\%_{\circ}Cl,\;7.14%_{\circ}Cl\;and\;11.05%_{\circ}Cl$, accoodation rate is a follow: accomodation capacity in ease of removing into higher chlorinities from lower chlorinities is increased in proportion as earlier stages, setting a conversional stage for eighth zoea stage, and by contraries, in case of advanced stages from eighth zoea it is incraesed in proportion as approach to post-larva stage in the case of transmigration into lower chlorinity from higher chlorinity. On the other hand, it is interesting that in case of reciprocal transmigration between two different chlorinitiess, each survival rate is different, and in this case, also, its accomodation in each zoea stage has a tendency to vary according to larval stages as described above, setting a conversional stage for eighth zoea stage. 6) It is likely that expension of radish pigments on body surface is directly proportional to chlorinity during the period of zoea rearing, and therefore it seems like all body surfacts of zoea larvae be radish coloured in case of higher chlorinity. 7) By the differences that each zoeal larvae, postlarvae, juvaniles and adult prawn are required different chlorinity for inhabiting in each, it is regarded that this species migrats from up steam to near the estuary of the river which the prawns inhabits commonly in natural field for spawning and growth migration. 8) It had better maintainning chlorinities according to zoeal stage for a comfortable method on seed-mass production that earlier larva stages than eighth zoea are maintained on the range from $8\%_{\circ}Cl\;to\;12\%_{\circ}Cl$ to rear, and later larva stages than eighth zoea, by contraries, are gradually regula ted-to love chlorininity of the range from $7\%_{\circ}Cl\;to\;4\%_{\circ}Cl$ according to advance for post-larva stage.

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Morphological Development of Eggs, Larvae and Juveniles of the Misgrunus anguillicaudatus (Cypriniformes: Cobitidae) (미꾸리 Misgrunus anguillicaudatus (Cypriniformes: Cobitidae)의 난발생 및 자치어 형태발달)

  • Park, Jae-Min;Yoo, Dong-Jae;Son, Jun-Hyeok;Han, Kyeong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.1
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    • pp.23-29
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    • 2022
  • This study was carried out to clarify the egg, larvae and juveniles development of Misgurnus anguillicaudatus, and relationships of M. anguillicaudatus and M. mizolepis, Cobitididae Fishes. The adult fishes were collected in Samsan-cheon, Haenam-gun, Jeollanam-do, Korea and their spawning inducement was carried by ovaprim injections. The egg shape was circular and the size was average 1.12 mm. The eggs were hatched at 61 to 72 h after fertilization. The newly hatched larvae had an average 3.23 mm in total length (TL). At 5 days after hatching, the larvae reached to post larval stage and they were 10.3 mm in TL. At 19 days after hatching, it reached to juvenile stage and was 25.3 mm in TL. The egg size of M. anguillicaudatus was almost same as M. mizolepis but the hatching period of M. anguillicaudatus has taken longer. It was possible for interspecific distinguishability of M. anguillicaudatus and M. mizolepis when their larvae reached to juvenile stage by the development of keel-like ridges.

Rapid Expression of Bm46 in Bombyx mori Cell Lines, Larvae and Pupae

  • Wang, Haiyan;Chen, Keping;Guo, Zhongjian;Yao, Qin;Wang, Qiang;Mu, Runhong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.15 no.1
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    • pp.35-38
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    • 2007
  • In this study, ORF 46 of Bombyx mod nucleopolyhedrovirus(Bm46) fused with EGFP was expressed in Bombyx mod cell lines, larvae and pupae by BmNPV Bacmid system. Bm46 and EGFP were cloned into donor plasmid pFastBacHTb, which was transformed to competent DH10B cells containing helper and BmNPV bacmid by site-specific transposition. Recombinant bacmid was used to transfected BmN-4 cells to produce the recombinant baculovirus vBm-Bm46-EGFP. Recombination virus was injected into silkworm larvae and pupae. The expression of the fusion protein was monitored by examining green fluorescence using a fluorescent microscope. Intense fluorescence in cells and silkworm was observed at 4 days post-infection, indicating the Bm46-EGFP fusion gene was expressed successfully.

A study on toxicity of several pesticides on larval development of shrimp cardina denticulata denticulata de haan (새뱅이 ( Caridina denticulata denticulata De Haan ) 의 유생 발생을 통한 수종 살충제 독성에 관한 연구)

  • Kim, Chang-Hyun
    • The Korean Journal of Ecology
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    • v.15 no.3
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    • pp.257-265
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    • 1992
  • The effects of insecticide dimilin including the technical grade(tg) and wettable power(wp-25), inhibiting the synthesis of chitin in the cuticle of insect larvae, and herbicides alachlor and atrazine on the development of shrimp caridina denticulata denticulata were investigated in various concentrations. This species is abundant in the freshwater such as river and pond. The shrimp larvae were reared at dimilin solutions(tg and wp 2), alachlor solutions, and atrazin solutions. the concentrations of dimiline were 0, 1, 5, 10 and 25 ppb dimilin solutions, and those of alachlor and atrazin were 0, 1, 5, 10 and 25ppm, respectively.mortality was high at 10 ppb when the larvae were exposed to dimilin and at 25ppm in the case of alachlor and atranzine solutions. If lethal concentration is defined as a concentration in which less than 10% of larvae to the post larval stage from hatching it can be concluded that the lethal concentration of dimilin is 5ppb, and that of alachlor and atrazin is 25 ppm.

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