• Proceedings of the Korean Society of Crop Science Conference
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• 2007.04a
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• pp.76.1-76.1
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• 2007

• Proceedings of the Korean Society of Crop Science Conference
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• 2003.05a
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• pp.63-64
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• 2003

• Korean Journal of Food Science and Technology
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• v.23 no.2
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• pp.133-140
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• 1991

Weight-loss, general-appearante, yellowing, and compositions such as chlorophyll, carotenoid, vitamin C and nitrate, were studied to investigate the influence of pre-(cultivar and growing season) and post-(low-temperature and high-density polyethylene film packaging) harvest factors on the shelf-life and quality of leaf lettuce(Lactuca sativa L). Weight-loss was reduced by the treatment of high-density polyethylene film packaging, and more at $0{\sim}1^{\circ}C$ than $4{\sim}5^{\circ}C$. Leaf-lettuce grown in Winter lost less fresh weight than that grown in Autumn. In experiment on difference between cultivars, the reduction of weight-loss in 'Cheongchima' lettuce was somewhat significant than that in 'Jeokchukmyeon' lettuce. The tendency of changes in general appearance was similar to that of weight-loss. The yellowing of leaf lettuce during the storage was caused by chlorophyll degradation. Vitamin C content before the storage was 128.0 mg/100 g FW. in lettuce grown in Winter, 123.8 mg/100g FW. in that grown in Autumn, and more 'Cheongchima' lettuce than 'Jeokchukmyeon' lettuce. In all treatment, vitamin C content decreased rapidly, and this tendency was more obvious at $4{\sim}5^{\circ}C$ than $0{\sim}1^{\circ}C$. The nitrate content of 'Jeokchukmyeon' was 3812.6 ppm and that of 'Cheongchima' was 2855.6 ppm before the storage. Nitrate content decreased more at $4{\sim}5^{\circ}C$ than $0{\sim}1^{\circ}C$ during the storage.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.569-573
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• 2010

A quantitative detection method for Salmonella in seafood was developed using a SYBR Green-based real-time PCR assay. The assay was developed using pure Salmonella DNA at different dilution levels [i.e., 1,000 to 2 genome equivalents (GE)]. The sensitivity of the real-time assay for Salmonella in seeded seafood samples was determined, and the minimum detection level was 20 CFU/g, whereas a detection level of 2 CFU/ml was obtained for pure culture in water with an efficiency of ${\geq}85%$. The real-time assay was evaluated in repeated experiments with seeded seafood samples and the regression coefficient ($R^2$) values were calculated. The performance of the real-time assay was further assessed with naturally contaminated seafood samples, where 4 out of 9 seafood samples tested positive for Salmonella and harbored cells <100 GE/g, which were not detected by direct plating on Salmonella Chromagar media. Thus, the method developed here will be useful for the rapid quantification of Salmonella in seafood, as the assay can be completed within 2-3 h. In addition, with the ability to detect a low number of Salmonella cells in seafood, this proposed method can be used to generate quantitative data on Salmonella in seafood, facilitating the implementation of control measures for Salmonella contamination in seafood at harvest and post-harvest levels.

• The Journal of Korea Institute of Information, Electronics, and Communication Technology
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• v.11 no.2
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• pp.161-168
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• 2018

In this study, IT based traceability system which is able to measure weight and moisture content of grain in the post-harvest process of intake, drying, storage and milling was developed in RPC(Rice processing complex). Measured information of weight, moisture content, yield, loss and quality was saved in the DB sever. Simultaneously, lot No. was generated and connecting to quality and traceability information. Also, automatic control system with MMI(Man Machine Interface) and yield and inventory control system(YICS) for grain was developed for the traceability system by applying the TCP/IP communication. In addition, simulation of system was performed for evaluation in RPC.

• The Korean Journal of Food And Nutrition
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• v.30 no.4
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• pp.635-643
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• 2017

The properties of rice were studied, and 8 rice flour cultivars were used to study the effect of particle size on the physicochemical properties, color value, RVA viscosities, water absorption index (WAI), and water solubility index (WSI) of rice flours. The mean particle size by the 3 particle size classification of 150 mesh, 200 mesh, and 250 mesh was, $90.75{\mu}m$, $60.73{\mu}m$, $39.94{\mu}m$, respectively. Thai rice had the highest amylose content and Samkwang rice had the lowest amylose content. Protein content of rice flours prepared was decreased as the particle size of rice flour decreased. In terms of color values, the L-value and the a-value of rice flour were increased as the particle size of rice flours decreased, while the b-value was decreased as the particle size of rice flours decreased. Using a rapid visco analyzer (RVA), the initial pasting temperature of Thai rice cultivar was found to be the highest; the peak viscosities of Sunpum cultivar and Misomi cultivar, and Samkwang rice were higher than those of other rice flours. The water absorption index and water solubility index were increased as the particle size of rice flour decreased. In order to use processed rice flour for the development of processed foods, proper characteristics of the cultivars and particle size should be considered.

• The Korean Journal of Food And Nutrition
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• v.34 no.4
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• pp.381-389
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• 2021

Saengcheonggukjang, known as Natto in Japan, is a soybean fermented food which is made from steamed soybean, Bacillus and water. Demand of Saengcheonggukjang has increased because it does not have much smell compared to Cheonggukjang. Seven varieties of Saengcheonggukjang were investigated and compared in terms of 100 seed weight, quality characteristics, hard seed rate, and water absorption rate in order to determine the suitability of Korean soybeans. In addition, each characteristic of Saengcheonggukjang was compared. 'Hoseo' and 'Haewon' showed low 100 seed weight with 8.41 g and 8.11 g, respectively. The water absorption rate was higher in 'Hoseo' and 'Pungwon' than in Japan varieties. The yield of Saengcheonggukjang was significantly different for each variety. No differences were observed in yield and hardness of Saengcheonggukjang with respect to the varieties and sowing date. 'Haewon' showed the highest amino nitrogen content with 575.0 mg%. Viscous material content did not appear to differ between varieties. These results suggested that 'Hoseo' and 'Haewon' can be considered as suitable candidates for yield and quality of Saengcheonggukjang compared to Japan varieties.

• Journal of Microbiology and Biotechnology
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• v.34 no.2
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• pp.457-466
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• 2024

Cellobiose dehydrogenases (CDHs) are a group of enzymes belonging to the hemoflavoenzyme group, which are mostly found in fungi. They play an important role in the production of acid sugar. In this research, CDH annotated from the actinobacterium Cellulomonas palmilytica EW123 (CpCDH) was cloned and characterized. The CpCDH exhibited a domain architecture resembling class-I CDH found in Basidiomycota. The cytochrome c and flavin-containing dehydrogenase domains in CpCDH showed an extra-long evolutionary distance compared to fungal CDH. The amino acid sequence of CpCDH revealed conservative catalytic amino acids and a distinct flavin adenine dinucleotide region specific to CDH, setting it apart from closely related sequences. The physicochemical properties of CpCDH displayed optimal pH conditions similar to those of CDHs but differed in terms of optimal temperature. The CpCDH displayed excellent enzymatic activity at low temperatures (below 30℃), unlike other CDHs. Moreover, CpCDH showed the highest substrate specificity for disaccharides such as cellobiose and lactose, which contain a glucose molecule at the non-reducing end. The catalytic efficiency of CpCDH for cellobiose and lactose were 2.05 × 10⁵ and 9.06 × 10⁴ (M^-1 s^-1), respectively. The result from the Fourier-transform infrared spectroscopy (FT-IR) spectra confirmed the presence of cellobionic and lactobionic acids as the oxidative products of CpCDH. This study establishes CpCDH as a novel and attractive bacterial CDH, representing the first report of its kind in the Cellulomonas genus.

• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2001.10a
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• pp.248-265
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• 2001

Recently, agriculture in Korea is seriously depressed by economic globalization and trade liberalization because of low competitive power. Organic farming, which is favorable for small scale agriculture, is expected to be one of alternatives to boost agricultural activities in Korea. Organic agriculture requires systemic developments of new technologies considering the complex biological interaction within the system in connection with high quality and safety standard. The National Institute of Agricultural Science & Technology(NIAST) has been worked on the series of organic farming technologies, including bio-pesticides, pheromone, natural enemies as well as the testings of materials used in organic farming. NIAST will concentrate on the technological development for compromising to the conflict between commodity-oriented and ecological harmony. Research on systemic land and water use, management of plant nutrition, pest and disease control, post-harvest would also be important targets. Integrated efforts among researchers, professors, administrators, farmers, private sectors, and extension workers are substantial for fast technology development and its dissemination. Development and use of these technologies will homely enlighten the Korean organic farming although it may require quite a time and endeavor.

• Journal of Biosystems Engineering
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• v.29 no.2
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• pp.187-194
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• 2004

The study focused on setting priority among the future core technologies that were derived by Delphi in agricultural management ＆ information using analytic hierarchy process(AHP). Items for setting priority were decided as ‘technology’, ‘market’, and ‘public concerns’by council. The future core technologies were divided into four parts by importance and R＆D level. Technologies which consisted of upper two parts having higher importance were considered in the study. Questionnaires were given to 9 specialists in biosystems engineering. As the results, ‘market’ was decided as a most important item. The most important technologies were ‘Developing technology and system of post harvest processing for higher quality rice’ in group I, and ‘Developing sensor technology to evaluate quality and safety for agricultural products’ in group II.