• Korean Journal of Animal Reproduction
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• v.25 no.4
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• pp.381-388
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• 2001

The present study was designed to obtain porcine growth hormone binding protein (pGHBP) improved biological activation as derived mutation in binding site with growth horlnone (GH). A 756 bp of fragment encoding the extracellular domain of pGHBP gene was cloned from the total RNA of porcine fat tissue by reverse transcriptase polymerase chain reaction (RT-PCR) and created mutation in positions 26 and 122 using site-directed mutagenesis method. Position 26 is one and it is near to get on five potential N-linked glycosylation sites located in the extracellular domain of porcine growth hormone receptor known to have a direct influence on combination with GH. Position 122 is known as one of conformational epitope in bovine. It was over-expressed in E. coli using pET-32(c) expression vector and precisely purified by S-protein agarose and enterokinase. In our results, we was obtained pmGHBP of 30 kDa. It suggests to study the effects of the pmGHBP on cell proliferation in vitro and growth rate in vivo after administration.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.12 no.3
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• pp.409-426
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• 2001

Absorbed power of the human head radiated from a 900 MHz portable phone and temperature rise are computed using FDTD(Finite-Difference Time-Domain) method. For this computation the 5 layered media for the human head modeling and the monopole antenna attached to metallic box for the portable phone are used. To reflect the real circumstances typical sizes of human heads and portable phones are considered in the calculation. The length of monopole antenna is 8.15 cm, and the output power of a phone is 600 mW. Under the predetermined model the distribution of 1 g, 10 g averaged SAR and temperature rise rate over the human head are calculated, from which it was found that the position of maximum SAR is near at the head skin surface, not deep places far into the head. The position of the highest temperature is located far from the head skin more than that of the maximum SAR occured. The averaged SAR and temperature along the distance between the head and phone are calculated according to seperation distance between the head and phone.

• Journal of the Korean Magnetic Resonance Society
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• v.9 no.2
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• pp.110-121
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• 2005

E.coli methionyl tRNA synthetase consist of 676 amino acids and plays a key role in initiation of protein synthesis. The native form of this enzyme is a homodimer, but the monomeric enzyme truncated approximately C-terminal 120 amino acids retains the full enzymatic activities. X-ray crystal structure of the active monomeric enzyme shows that it has two domains. The N-terminal domain is thought to be a binding site for acceptor stem of tRNA, ATP, and methionine. The C-terminal domain is mainly a-helical and makes an interaction with the anticodon of $tRNA^{Met}$. Especially it is suggested that the region of helix-loop-helix including the tryptophan residue at the position 461 may be the essential for the interaction with anticodon of $tRNA^{Met}$. In this work the structure and function of E. coli methionyl-tRNA synthetase was studied by spectroscopic method (NMR, CD, Fluorescence). The importance of tryptophan residue at the position 461 was investigated by fluorescence spectroscopy. Tryptophan 461 is expected to be an essential site for the interaction between E. coli methionyl-tRNA synthetase and E. coli $tRNA^{Met}$. Proton and heteonuclear 2-dimensional NMR spectroscopy were also used to elucidate the protein-tRNA interaction.

• Journal of Gifted/Talented Education
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• v.10 no.2
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• pp.47-69
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• 2000

One of the most controversial issues in contemporary research of creativity, whether a person"s creativity is domain-specific or domain-general, was investigated with 109 second grade children in the present study. The purposes of this study sere to empirically examine (1) the relationships among children's creative performances measured by three product-based assessments (story-telling, collage-making, and math word problems) in three domains, and (2) the relationships between children's general creative thinking sills, measured by two divergent thinking tests, and children's creative performances. The findings of this study support the position that creative ability in young children is rather (but not absolutely0 domain-specific. Children exhibited a range of creative ability across different domains rather than a uniform creative ability in diverse domains, indicating there is considerable intra-individual variation in creative ability by domain. Divergent thinking measures did not have great power in predicting creative performance in at least two of three, if not all, domains assessed in the study. It is implied from the study that it is not possible to reliably predict a child"s creative ability in one domain based on his/her creative ability in other domains or his/her overall divergent thinking ability. Implication of the study in connection with educational practices for gifted children is discussed.

• Journal of Positioning, Navigation, and Timing
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• v.4 no.1
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• pp.33-41
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• 2015

Compact Network Real-Time Kinematic (RTK) is a method that combines compact RTK and network RTK, and it can effectively reduce the time and spatial de-correlation errors. A network RTK user receives multiple correction information generated from reference stations that constitute a network, calculates correction information that is appropriate for one's own position through a proper combination method, and uses the information for the estimation of the position. This combination method is classified depending on the method for modeling the GPS error elements included in correction information, and the user position accuracy is affected by the accuracy of this modeling. Among the GPS error elements included in correction information, tropospheric delay is generally eliminated using a tropospheric model, and a combination method is then applied. In the case of a tropospheric model, the estimation accuracy varies depending on the meteorological condition, and thus eliminating the tropospheric delay of correction information using a tropospheric model is limited to a certain extent. In this study, correction information modeling accuracy performances were compared focusing on the Low-Order Surface Model (LSM), which models the GPS error elements included in correction information using a low-order surface, and a modified LSM method that considers tropospheric delay characteristics depending on altitude. Both of the two methods model GPS error elements in relation to altitude, but the second method reflects the characteristics of actual tropospheric delay depending on altitude. In this study, the final residual errors of user measurements were compared and analyzed using the correction information generated by the various methods mentioned above. For the performance comparison and analysis, various GPS actual measurement data were collected. The results indicated that the modified LSM method that considers actual tropospheric characteristics showed improved performance in terms of user measurement residual error and position domain residual error.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.4 no.6
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• pp.1063-1079
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• 2010

Vehicle ad hoc networks (VANET) are one of the most important technologies to provide various ITS services. While VANET requires rapid and reliable transmission, packet transmission in VANET is unstable because of high mobility. Many routing protocols have been proposed and assessed to improve the efficiency of VANET. However, topology-based routing protocols generate heavy overhead and long delay, and position-based routing protocols have frequent packet loss due to inaccurate node position. In this paper, we propose a position-based routing repair algorithm to improve the efficiency of VANET. This algorithm is proposed based on the premise that AODV (-PGB) can be used effectively in VANET, if the discovery, maintenance and repair mechanism of AODV is optimized for the features of VANET. The main focus of this algorithm is that the relay node can determine whether its alternative node exits and judge whether the routing path is disconnected. If the relay node is about to swerve from the routing path in a multi-hop network, the node recognizes the possibility of path loss based on a defined critical domain. The node then transmits a handover packet to the next hop node, alternative nodes and previous node. The next node repairs the alternative path before path loss occurs to maintain connectivity and provide seamless service. We simulated protocols using both the ideal traffic model and the realistic traffic model to assess the proposed algorithm. The result shows that the protocols that include the proposed algorithm have fewer path losses, lower overhead, shorter delay and higher data throughput compared with other protocols in VANET.

• Journal of Ocean Engineering and Technology
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• v.33 no.2
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• pp.99-105
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• 2019

Unlike typical a dynamic positioning (DP) system, a DP-assisted mooring system must determine a set point (SP) that can ensure a mooring tension safety range to prevent an excessive increase in mooring tension. In this paper, a new algorithm for determining the SP is suggested in order to reduce the tension on all the mooring lines. To determine the SP, a working area around the vessel is represented by a rectangular grid. Thus, the size of the grid area is limited considering the offset of a vessel with a mooring system. At each grid's nodes, the resultant tension from all the mooring lines is estimated using the time history of the tension and vessel's position. The results of static analyses for each grid position are used to estimate the global tension. Consequently, the SP is automatically selected as a position satisfying criterion for minimizing the total tension. In order to validate the suggested algorithm, a motion simulation with the control system in the time domain and a discussion of the results are presented.

• Journal of Gifted/Talented Education
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• v.15 no.2
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• pp.1-34
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• 2005

This study is composed of 2 parts. Study 1 empirically examined (1) the relationships among children's creative performances measured by three product-based assessments (story-telling, collage-making, and math word problems) in three domains, and (2) the relationships between children's general creative thinking skills, measured by two divergent thinking tests, and children's creative performances. Study 2 is a three-year follow up study of the study 1. Study 2 followed up some (71) children who participated in study 1. In study 2, long-term stability of the performance based assessment involving story-telling, collage making, and math problem making were examined during the three-year time period. In addition, study 2 tried to look at the methods effect of the domain issue of creativity, comparing self-report scale and performance-based assessment. The findings of this study support the position that creative ability in young children is rather (but not absolutely) domain-specific. The long-term stability of the performance-based assessments compares favorably with stability figures for other creativity tests. Results also indicate that there are some method effect in explaining the domain issue of creativity. Implication of the study in connection with educational practices for gifted children is discussed.

• Journal of Life Science
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• v.28 no.4
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• pp.408-414
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• 2018

Actein is the well-known active ingredient of Cimicifugae rhizoma (Black cohosh). In this study, we investigated and compared the binding affinity of tubocurarine, actein, and actein derivatives on the B&C domain of the acetylcholine binding protein through in silico computational docking studies. The three-dimensional crystallographic structure of the acetylcholine binding protein B&C domain was obtained from the PDB database (PDB ID: 2XYT). An in silico computational autodocking analysis was performed using PyRx, Autodock Vina, Discovery Studio Version 4.5, and NX-QuickPharm based on scoring functions. The actein showed an optimum binding affinity (docking energy), with the acetylcholine binding protein at -10.50 kcal/mol as compared to the tubocurarine (-9.80 kcal/mol). The interacting amino acids tryptophan 84 and tryptophan 147, in the B domain of the acetylcholine binding protein active site, significantly interacted with the actein and 27-deoxyactein, and (26R)-actein. The centroid XYZ grid position of the tubocurarine was X=38.300689, Y=112.053467, and Z=51.991022, but the actein and its derivatives showed values around X=26.4, Y=127.3, Z=43.7. These results clearly indicated that actein and its derivatives could be a more potent antagonist to the acetylcholine binding protein than tubocurarine. Therefore, the extract of Cimicifugae rhizoma or actein containing biomaterials can substitute for the botulinum toxin-mediated acetylcholine receptor regulation, and be applied to the anti-wrinkle cosmetics industry.

• Journal of Positioning, Navigation, and Timing
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• v.3 no.1
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• pp.25-30
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• 2014

Differential Global Positioning System-Correction Projection (DGPS-CP) algorithm, which has been suggested as a method of correcting pre-calculated position error by projecting range-domain correction to positional domain, is a method to improve the accuracy performance of a low price GPS receiver to 1 to 3 m, which is equivalent to that of DGPS, just by using a software program without changing the hardware. However, when DGPS-CP algorithm is actually realized, the error is not completely eliminated in a case where a reference station does not provide correction of some satellites among the visible satellites used in user positioning. In this study, the problem of decreased performance due to the difference in visible satellites between a user and a reference station was solved by applying the Multifunctional Transport Satellites (MTSAT) based Augmentation System (MASA) correction to DGPS-CP, instead of local DGPS correction, by using the Satellite Based Augmentation System (SBAS) operated in Japan. The experimental results showed that the accuracy was improved by 25 cm in the horizontal root mean square (RMS) and by 20 cm in the vertical RMS in comparison to that of the conventional DGPS-CP.