• 한국식품영양과학회지
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• 제36권10호
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• pp.1314-1319
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• 2007

본 연구는 일반 김과 기능성 김의 성분과 관능평가를 비교하기 위하여 전남 신안군에 위치하고 있는 김 제조업체에서 제품을 제공받아 일반 김, 목초액 김, 함초 김, 퉁퉁마디 참숯돌김의 일반성분, 무기질 함량, 중금속 함량, 구성아미노산 분석 및 김을 굽기 전과 구운 후의 관능평가를 실시한 결과는 다음과 같다. 일반성분은 탄수화물과 조 섬유 함량을 제외하고는 일반 김에 비해 기능성 김에서 높게 나타났다. 무기질 함량에서 칼슘, 칼륨, 구리, 나트륨 함량은 함초 김, 철분 함량은 퉁퉁마디 참숯돌김에서 가장 높게 나타났으며 마그네슘과 아연을 제외하고는 기능성 김에서 높게 나타났다. 중금속 함량의 경우 크롬, 카드뮴, 납은 일반 김에서 높았고, 그 외 성분은 퉁퉁마디 참숯돌김에서 약간 더 높게 나타났으나 유의적인 차이는 없었다. 구성아미노산에서 일반 김과 기능성 김에서 대부분 glutamic acid, glycine, isoleucine, phenylalanine이 가장 많이 함유되어 있었으며, 특히 일반 김에 비하여 기능성 김에서 약 3배 정도 많이 나타난 아미노산은 alanine, phenylalanine이었으며, 일반 김과 목초액 김에 비하여 함초 김과 퉁퉁마디 참숯돌김은 serine과 lysine의 함량이 높게 나타난 것을 알 수 있었다. 관능평가결과에서는 김을 굽기 전에는 일반 김과 기능성 김 간의 차이는 없었으나 김을 구운 후에는 일반 김에 비하여 대부분의 기능성 김의 관능평가 결과가 유의적으로 높아졌음을 알 수 있었으며, 특히 목초액 김에 비하여 함초 김의 점수가 더 높게 나타났다.

• ALGAE
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• 제18권1호
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• pp.89-94
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• 2003

• ALGAE
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• 제17권2호
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• pp.89-93
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• 2002

Seasonal changes of pigment compositionin two intertidal red algae, Gloiopeltis furcata (Postels et Ruprecht) J. Agardh, and Porphyra yezoensis Ueda, were investigated. Chlorophyll α and phycoerythrin levels were high during winter, but decreased in late spring or summer, with accompanying discoloration from deep red to green or yellow. This discoloation corresponded closely to the fluctuationof phycoerythrin content. Nevertheless, photosynthesis capacity was maintained by the increasing water temperature in the field, suggesting that large amounts of phycoerythrin are not necessary for photosynthesis. Phycoerythrin conten correlated significantly with nitrogen content in both species when the nitrogen level was greater than the level of critical content (1.30% DW in G. furcata, and 2.26% DW in P. yezoensis), indicating that phycoerythrin plays a more important role in the nitrogen status as a nitrogen pool than that of nitrogen critical content. Furthermore, the dependence level of the alage on phycoerythrin as a nitrogen pool was greater in P. yezoensis than in G. furcata because of the remarkable increase of phycoerythrin content in P. yezoensis with increasing jnitrogen content.

• ALGAE
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• 제18권1호
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• pp.95-100
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• 2003

• Fisheries and Aquatic Sciences
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• 제15권1호
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• pp.77-81
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• 2012

We assessed the toxicity of cryoprotectant agents (CPAs) to gametophytic thalli of red alga Porphyra yezoensis at room temperature. The CPAs used were: dimethyl sulfoxide (DMSO), ethylene glycol (EG), glycerol (GC), 1,2-butanediol (1,2-BD), 1,3-butanediol (1,3-BD), 2,3-butanediol (2,3-BD), 1,3-propanediol (1,3-PD) and propylene glycol (PG). CPA concentrations of 10, 15, 20, 25, 30, 35, 40, 45, and 50% were employed with 30 or 60 s immersion. The toxicity of the eight CPAs to gametophytic thalli of P. yezoensis was in the order: 1,3-BD < DMSO ${\approx}$ 2,3-BD ${\approx}$ PG ${\approx}$ EG < GC < 1,3-PD ${\approx}$ 1,2-BD. All thalli were more sensitive to high CPA concentrations, and most (>75%) thalli survived exposure to 10-25% CPA for 60 s. These data will facilitate selection of the optimal cryoprotectant concentration for cryopreservation of P. yezoensis thalli.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2000년도 춘계수산관련학회 공동학술대회발표요지집
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• pp.151-152
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• 2000

During in door or outdoor mass culture, Porphyra have been easily contaminated with bacteria, protozoa and microalgal species. Several axenic treatments for Porphyra thalli have been published (Polne-Fuller and Gibo 1984; Chen and McCracken 1993), but axenic techniques for neutral spores and conchocelis we not developed. In this work we describe the procedure for axenic isolation of neutral spores and conchocelis of Porphyra yezoensis (omitted)

• ALGAE
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• 제20권3호
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• pp.183-196
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• 2005

Nuclear SSU and ITS1 rDNA and plastid rbcL sequences were determined to identify the seven samples of Porphyra cultivated by means of natural seeding on the southwest coast of Korea and analyzed to access the phylogenetic relationships of them with the natural populations of P. tenera and P. yezoensis from Korea and Japan. SSU, rbcL and ITS1 data from 18, 21 and 31 samples, respectively, including previously published sequences were investigated in the study. Results from our individual and combined data indicated that the seven samples were all P. yezoensis and the entities except one from Muan 2 aquafarm strongly grouped together with the natural populations of P. yezoensis from the south and the west coast of Korea. The sample from Muan 2 seems to be derived from a strain of P. yezoensis introduced from Japan by Porphyra farmers, based on DNA sequence data.

• Fisheries and Aquatic Sciences
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• 제8권2호
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• pp.51-55
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• 2005

We measured the anti-attachment activity of allelochemical and antifouling substances using monospores from Porphyra yezoensis Ueda as an assay. Methanol or aqueous extracts ($20/{\mu}g/mL$) from 32 seaweeds were added to monospore suspensions. Methanol extracts of Corallina pilulifera, Ishige sinicola, Sargassum horneri, and Sargassum sagamianum inhibited attachment $by\;>90\%$ compared to the reference. Phenolic compounds fractionated from S. sagamianum caused the most potent inhibition. P. yezoensis monospores also showed significant sensitivity to known allelochemical and algicidal compounds.

• 생명과학회지
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• 제9권5호
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• pp.531-536
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• 1999

As an attempt to preserve resources in marine biological organisms, we first constructed a cDNA library from the red alga Porphyra yezoensis. The library construction method from P. yezoensis consists of three steps; those include protoplast presparation, RNA isolation, and phage library construction. Protoplast was prepared in order to remove much of the carbohydrate compounds which are characteristics of algal cell walls. Carbohydrate contamination in the purified RNA may inhibit further enzyme reactions, those carbohydrates should be removed. RNA samples prepared from protoplast still seemed to contain residual amount of carbohydrate because mRNA isolation with conventional method failed. We therefore developed a method with Poly ATtract mRNA isolation system. The constructed phage library was tested by analyzing cDNA insert in phage vector from randomly picked ten independent white plagues. All of the phages contained cDNA inserts with sizes ranging 0.5kb and 2.0kb.

• ALGAE
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• 제18권1호
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• pp.83-88
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• 2003