• Environmental Analysis Health and Toxicology
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• v.11 no.3_4
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• pp.59-63
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• 1996

In present study, we fractionated polysaccharides from Croton tiglium and investigated their hepatoprotective effects on CCl$_4$ intoxication. Polysaccharide fraction of which molecular weight is over 300,000 (HP) showed the most potent hepatoprotective effects on CCl$_4$ intoxication. Lipid peroxidation, sAST and sALT were used as parameters to evaluate the liver damage. Glucose, xylose and arabinose were found to be monosaccharides composing sugar moiety of HP.

• KSBB Journal
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• v.13 no.3
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• pp.303-307
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• 1998

The fermentation conditions for the maximal production of the useful polysaccharides(water soluble polysaccharide and cell bound polysaccharide) from marine bacterium Zoogloea sp.(KCCM 10036) were investigated with a 5 L jar fermentor. The maximal production of these polysaccharides was obtained under the conditions of initial pH 7.8, 30$^{\circ}C$, 400 rpm of agitation speed, 2 Wm of aeration rate, 10%(w/v) of inoculum size and 2.5%(w/v) of glucose substrate and 10.38 g/L of total polysaccharide was produced. Apparent viscosity of the culture broth was increased with the production of these polysaccharides and the maximum value was reached to 22,500 cp.

• Food Science and Preservation
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• v.4 no.3
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• pp.301-309
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• 1997

This study was conducted to examine morphological characteristics and the content of cell wall polysaccharides of Brassica campestris var. pekinensis(baechu). First of all, the variety of scientific name and naming of parts of baechu in the literatures of kimchi showed, which will unify marks. So, we propose not so much mid-rib and leaf blade of baechu leaf as white part and green part, respectively. On the other hand, the forms of vessel elements of white part in baechu consist in ring, sclariform and reticulate thickening. The proximate compositions and contents of cell wall polysaccharides of baechu has significant differences between its cultivars. The cell wall pectin from baechu exhibited four peals with molecular weights of 2,000,000, about 100,000 and less than 10,000 by gel filteration chromatography and hemicellulose did two peaks with molecular weights of 2,000,000 and 10,000.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.40 no.2
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• pp.68-72
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• 2007

The flow properties of sulfated polysaccharides purified from the ascidian Halocynthia roretzi tunic were investigated. The sulfated polysaccfarides produced aqueous solutions of low apparent viscosity with pseudoplastic flow behavior. The respective activation energies of TCA-treated and TCA-nontreated solutions were $2.2248{\times}10^4\;and\;1.442{\times}10^4J/kg{\cdot}mol$ at a 150L/s shear rate. The viscosity of the sulfated polysaccharides solution was increased by the addition of sugar, while it was not changed by the addition of NaCl.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.812-815
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• 2005

The basidomycetes fungus, Agaricus blazei Murill has been well known as the biologically active polysaccharides. They do not attack cancer cells directly, but produce their antitumor effects by activating various immune responses in the host. This present study was designed to investigate the immune activity of polysaccharides extracted from fruiting body, culture broth, and mycelia. In order to examine the optimum conditions of polysaccharide production, medium and C/N ratio were examined in flask culture. The fine polysaccharides were isolated and purified by DEAE cellulose and Sephadex CL-6B, respectively. Each fraction was spplied to recognize NO synthesis and TNF-${\alpha}$ for immune activity test.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.854-858
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• 2005

Methanol and water crude extracts, crude polysaccharides and crude polysaccharides-free of water crude extracts from Shiitake mush-room (Lentinus edodes) were investigated for their antioxidant capacity in total polyphenolics and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Total polyphenolics in the crude polysaccharides-free of water crude extracts were higher than that of the other ones. And crude polysaccharides-free of water crude extracts and water crude extracts show high on DPPH free radical scavenging activity. Positive correlations were found between total polyphenolics and their antioxidant activity.

• Journal of Microbiology and Biotechnology
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• v.14 no.1
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• pp.202-204
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• 2004

Polysaccharides and saponins were isolated from the root of Panax ginseng C.A. Meyer (Family Araliaceae), treated at various temperatures, and their inhibitory effects on rotavirus were investigated. As the temperature of processing increased, the molecular weight of the polysaccharides decreased, but the yields of water extracted increased. These polysaccharides inhibited rotavirus infection in MA104 cells, but there were no significant differences in rotavirus infection-inhibitory potency. However, ginseng saponins did not exhibit rotavirus infection-inhibitory activity.

• Journal of the Korean Wood Science and Technology
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• v.26 no.3
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• pp.26-32
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• 1998

The chemical composition of differentiating xylem of Populus deltoides M. were investigated and compared with those from sapwood. The cell wall polysaccharides were extracted sequentially from a differentiating xylem and sugar composition was analyzed with G.L.C, H.P.L.C and gel chromatograpy. The pectin substance and hemicellulose are rich in the cell wall of differentiating xylem. The $H_2O$ extract polysaccharides from differentiating xylem were composed with xylose-glucose residues which seem to be xyloglucan and a pectin. The arabinogalactan and the mannan were extracted with $Na_2CO_3$ solution and also the xylan was extracted with KOH solution. Sugar composition of each fractions in gel filteration of purified $H_2O$ polysaccharide suggests that the xyloglucan can be extracted with $H_2O$ from differentiating xylem.

• Archives of Pharmacal Research
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• v.8 no.1
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• pp.42-44
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• 1985

Polysaccharides were isolated with alkaline extraction method from twelve pharmaceutical plants, which have been used against the various tumors in the oriental herb medicine, and examined for their antitumor activities. When the polysaccharides were administered i. p. at the dose of 10mg/kg/day for ten consecutive days to the male ICR mice, which had been implanted with $1{\times}10^{6}$ cells of sarcoma 180 twentyfour hours before the first injection of polysaccharides, those from Forsythia Corea, Curcuma, Zedoaria, Albizzia Julibrissin, Prunuts Persica, Foeniculum Vlugare and Daphne Pseudogenkwa showed inhibition ratios of 88.0%, 61.1%m 73.0%, 72.8% 55.1% and 71.7%. The significant prolongation of life span was observed only in the case of Forsythia Corea (18.1%). Other six polysaccharide fractions from Olibanum, Lonicera Japonica, Rheum Coreanum, Scirpus Maritimus, Gleditchia Officinalis and Brassica Juncea showed negligible inhibition ratios.

• The Korean Journal of Mycology
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• v.27 no.2 s.89
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• pp.100-106
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• 1999

The polysaccharides, intracellular and extracellular, extracted from the liquid culture of the Agrocybe cylindracea were purified and characterized. The mycellial cellular productivity of Agrocybe cylindracea was proved to be almost 2 folds in the shaking culture compared to the standing culture. These polysaccharides were purified by the DEAE-cellulose ion exchange chromatography and the Sepharose 2B size exclusive gel filteration. The two purified fractions of extracellular polysaccharides, ACEPDG and ACEPAG, contained 75.8% and 65.4% total sugar respectively. The total sugar content of ACIPDG and ACIPAG, the two purified fractions of intracellular polysaccharides, were 89.2% and 54.2% respectively. The molecular weights range of all the substances were estimated to be above 100,000, from 300KDa of ACEPDG to 600 KDa of ACIPAG. The results of sugar analysis by HPLC showed that the sugar part of ACEPDG was consisted of glucose and inositol. The ACIPDG, ACEPAG and ACIPAG contained three kinds of monosaccharides, glucose, fructose and inositol.