• 한국자원식물학회지
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• 제3권1호
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• pp.1-30
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• 1990

The traditional plant imprownent methods consisted of pure line selection, cross breeding, heterosis breeding, polyploid breeding, mutati-onbreeding, ect.Biotechmoiogy is divided into gene spliclng , monocle-nal antibodies , protein engineering , agricultural research, and microbiological engineering. Of these , high plants deal with agricultural research, and the importent part of which is tissue culture and celLculture , Tissue .culture and cell culture are again divided into embryoculture, test tube fertilization, anther and pollen culture, somatichybridization , transformation, recombination, recombinant DNA moleculehybrid plasmid, ect For these haploid production, protoplast culture,protoplast fusion, selection and propagation, ect. , the technical sett-lement is needed.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.268-269
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• 2010

• 한국작물학회지
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• 제60권1호
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• pp.107-113
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• 2015

This study was conducted to find out the effective induction method of tetraploid plants to obtain potential data for cultivating superior varieties by colchicine treatment. The seed germination were decreased by the higher concentration of colchicine treatment and longer soaking time. A total of 907 individuals were germinated in 16 treated plots except control (untreated plot) and 28 tetraploids were induced which was about 3.1% of the number of seed germinated. The plant regeneration rate by colchicine treatment on explant of Prunella vulgaris for. albiflora Nakai under in vitro culture was decreased with the higher concentration of colchicine. While a total of 312 individuals were regenerated in all treatments, the explant was soaked in more than 0.05% for over 1 hour, tetraploid could be obtained. In particular, for the soaking treatment in 0.05% for 6 hours and 12 hours, 37 tetraploids were induced, which was about 57.8% of the number of plant regenerated. In accordance with the observation on doubling of DNA contents in leaf in order to identify polyploid, the peak DNA content of G1 phase was 101.3 for diploid and 197.2 for tetraploid. The result confirmed the doubling of DNA content. Furthermore, the number of chloroplasts per guard cell depending on polyploid was around 10 in diploid and 19.3 in tetraploid, which was around 1.9 times as much as diploid.

• 한국미생물·생명공학회지
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• 제24권5호
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• pp.604-612
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• 1996

To improve the fermentation characteristics of the haploid starch-fermenting recombinant yeast strain K114/YIpMS$\Delta$R(LEU2/URA3) secreting both $\alpha$-amylase and glucoamylase was rare-mated with polyploid industrial yeast Saccharomyces sp. K35. The K35 strain had good fermentation-characteristics such as ethanol-tolerance, high temperature and sugar-tolerance, and high fermentation rate. Among the resulting 66 hybrids, the best strain RH51 was selected. The RH51 exhibited amylolytic activity of K114/YIpMS$\Delta$R(LEU2/URA3) as well as ethanol and sugar tolerance of K35. The optimum temperature of hybrid RH51 for starch fermentation was 34$\circ$C which was same as that of K35 but different from that (30$\circ$C) of K114/YIpMS$\Delta$R(LEU2/URA3). The optimum pH was 5.0. The optimum size of inoculum was 2% as the pellet (w/v) of yeast cells. The hybrid strain RH51 produced 7.0% ethanol (w/v) from 20% (w/v) soluble starch while K35 formed almost no ethanol, 0.3% (w/v). RH51 strain produced 7.5% (w/v) ethanol after 8 days in a 2.5 l fermenter containing 800 ml of 20% (w/v) soluble starch. The residual starch content in the fermentation medium was 1.68% (w/v), and therefore almost all the starch was fermented completely.

• Journal of Plant Biotechnology
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• 제6권4호
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• pp.247-252
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• 2004

In vitro cultures of watermelon were treated with antimitotic agent colchicine to induce ploidy alterations, particularly the induction of tetraploids. Explants cotyledon, embryonic end of seed, transverse sections of epicotyl and hypocotyl were cultured on MS media supplemented with BA ($1{\mu}M$) and colchicine ($0.01\%,\;0.05\%\;and\;0.1\%$). Explants were subcultured on colchicine free media after 4 and 7 days. Colchicine had negative effect on in vitro regeneration but this exhibited explants related response. However, hypocotyl section of seedlings induced maximum callus on $0.01\%$ colchicine. Shoot proliferation was more in cotyledon explants cultured on colchicine ($0.01\%$) for four days. Maximum root induction and root number were recorded in embryonic end explants. Overall, cotyledon and embryonic end explants, and low colchicine concentration ($0.01\%$) was found optimal in watermelon regeneration.

• 생약학회지
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• 제2권1호
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• pp.29-34
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• 1971

A comparative study on Angelica and Cnidium, the two genera of Umbelliferae growing in Korea, was carried about the cytology and fertility of pollen. The somatic chromosome number of Umbelliferae was found almost to be 2N=22. Both species of Kang-whal and Chun-gung were found to be in polyploid with different chromosome numbers each other. The pollen fertility among the species of Angelica was high, but Toh-chun-gung was zero percent. The species of Angelica have been generated with pollen fertility, but species of Cnidium with only root or rootlet.

• 한국가금학회지
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• 제15권2호
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• pp.67-71
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• 1988

백색 Leghorn 암탉의 난자생성과정 중 감수분열을 억제하여 2배수성 난자(2n)의 생산에 근거한 다배교성 가축생산과 능력개양의 기초연구인 본 실험에서 감수분열시기는 배난 전 2~4시간이고 이때에 체중 kg당 0.3mg의 TEM(Tri ethylene melamin)을 복강에 주입하여 수정율 38%의 계란을 얻었고 이들 중 66%가 TEM의 영향으로 2n 의 난자가 생성되어 정상정자와의 결합으로 3배수성(3n)의 배아로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권3호
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• pp.455-462
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• 2003

Biotechnological research and development are moving at a very fast rate. The subject has assumed greatest importance in recent years in the development of agriculture and human health. The science of biotechnology has endowed us with new tools and tremendous power to create novel genes and genotypes of plants, animals and fish. The application of biotechnology in the fisheries sector is a relatively recent practice. Nevertheless, it is a promising area to enhance fish production. The increased application of biotechnological tools can certainly revolutionise our fish farming besides its role in biodiversity conservation. The paper briefly reports the current progress and thrust areas in the use of synthetic hormones in fish breeding, production of monosex, uniparental and polyploid individuals, molecular biology and transgenesis, biotechnology in aquaculture nutrition and health management, gene banking and the marine natural products.

• 한국잠사곤충학회지
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• 제44권2호
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• pp.56-58
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• 2002

뽕잎 두께가 비교적 두껍고 수량성이 높은 후엽녹의 생장점에 Colchicine용액 적하처리를 하여 창성한 4배성인 4원뽕 28호의 주요 특성은 다음과 같다. 1. 체세포의 염색체수는 2n=56인 4배체이다. 2. 봄철 발아개엽기는 중생뽕이고 잎모양은 삼각형에 가까운 타원형으로 폭광형이고 잎두께는 매우 두께우며 잎면적당 염록소 함량이 많다. 3.가지길이는 비교적 짧고 가지수도 다소 적으며 가지굵기는 굵은 편이고 기부불발아장비율이 높고 내동성은 약하다.

• 생약학회지
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• 제1권1호
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• pp.19-24
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• 1970

A comparative study on 'Dang-gui' and 'Kang-whal' the two species of Angelica growing in Korea was carried out about the morphology and cytology. The somatic chromosome number of Cham dang-gui, Ill dang-gui, Badi-namul was found to be 2N=22. The somatic chromosome number of Kang-whal was found to be 2N=110, that of Chili kang-whal to be 2N=44, that of Mul kang-whal to be 2N=22. Stoma of species of Dang-gui were same in size. Those of Chili kang-whal and Kang-whal were strikingly larger than the other. Chili kang-whal and Kang-whal were verified to be polyploid in the genus Angelica.