• 미생물학회지
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• 제29권5호
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• pp.296-300
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• 1991

Determent 들을 이용하여 대장균의 막에 걀합된 수소발생효소를 부분정제하였다.

• Journal of Plant Biology
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• 제33권4호
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• pp.277-283
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• 1990

Inactivation and reactivation of photosynthetic oxygen evolving complex were studied with isolated spinach (Spinacia oleraceda. L.) photosystem II particles by the activity of oxygen evolution and chlorophyll fluorescence. When the particles were treated with Tris and urea, the oxygen evolution was inactivated and three polypeptides having molecular weights of 33 kDa, 24 kDa and 18 kDa were simultaneously released. But in NaCl-treated particles, two polypeptides of 24 kDa and 18 kDa were removed from PS II particles. The oxygen evolution activities of Tris and urea-treated particles were not restored by adding cation ions (Mg2+, Mn2+ and Ca2+), but the NaCl-treated particles were restored by exogenously added Ca2+. The removal of these extrinsic polypeptides, especially 33 kDa, markedly showed the decrease of the variable fluorescence (Fv). These results are likely to be due to dissipate thermal energy by antenna of photosystem II complexes.

• 약학회지
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• 제27권4호
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• pp.315-320
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• 1983

Ginseng proteins have been extracted and partially purified by the methods of ammonium sulfate fractionation, heat inactivation and Sephadex G-75 column chromatography. The final three fractions obtained (GI, GII and GIII) were subjected to paper chromatography and SDS-polyacrylamide gel electrophoresis. Molecular weights of polypeptide chains from each fraction were est-mated by the standard line obtained from the plot of electrophoretic mobilities against the logarithm of molecular weights of standard proteins. Results are as follows: 1) GI showed three protein spots and four polypeptides of which M.W. were 63,000, 60,000, 56,000, 51,000 and 34,200. 2) GII showed four protein spots and five polypeptides with their M.W. of 64,600, 56,000, 45,400, 35,800, end 25,200, 3) GIII showed three protein spots and four polypeptides with their M.W. of 66,000, 63,000, 56,000 and 22,000.

• Applied Biological Chemistry
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• 제35권6호
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• pp.485-489
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• 1992

식물은 자극이 큰 환경적 stress에 반응 외견상의 변모 뿐 아니라 내적으로 생리적 생화학적인 변화가 있게 되며, 특히 체내의 단백질 합성계는 그러한 stress에 더욱 급격하게 반응한다. 여러가지 stress하에서 유묘기 벼의 단백질 수준이 어떻게 변화되는 가를 일차적으로 조사하기 위하여 NaCl, 한발 및 온도를 각각 달리 처리하여 이에 따른 폴리펩티드의 변이 양상을 전기영동에 의하여 비교하여 보았다. 환경 변화에 내성이 다소 강한 벼의 경우에도 stress 처리에 따라 다수의 폴리펩티드가 변화되었으며, 새로운 폴리펩티드의 생성도 관찰 되었다. 또한 기존의 1차 대사과정을 위한 단백질의 감소도 각각의 처리에 따라서 달리 표현되는 것이 확인되었다.

• Asian Pacific Journal of Cancer Prevention
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• 제14권12호
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• pp.7339-7344
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• 2013

To analyze the effects of a new unknown peptide DEF on the growth of tumor cells, a fused polypeptide TAT-DV1-DEF was designed and synthesized. The lung adenocarcinoma cell line GLC-82 treated with TAT-DV1-DEF was analyzed with a cell counting kit 8, and the location of polypeptides in cells was observed under laser confocal microscopy. The efficiency of polypeptide transfection and changes in nuclear morphology were analyzed by flow cytometry and fluorescence microscopy, respectively. Finally, the mechanism of tumor cell growth inhibition was evaluated by Western blotting. We found that TAT-DV1-DEF could significantly inhibit the growth of the lung adenocarcinoma cell line GLC-82, but not the normal human embryonic kidney cell line HEK-293. Polypeptides were found to be mostly localized in the cytoplasm and some mitochondria. The efficiency of polypeptide transfection in the two cell types was approximately 99%. Apoptotic nuclei were observed under fluorescence microscopy upon treatment with polypeptides and DAPI staining. Western blot analyses indicated that the polypeptide inhibition of tumor cell growth was apoptosis dependent. In the present study, we demonstrated that fused polypeptides could induce apoptosis of the lung adenocarcinoma cell line GLC-82, indicating that the new unknown peptide DEF has antitumor effects.

• Journal of Ginseng Research
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• 제16권2호
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• pp.124-128
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• 1992

인삼의 엽소현상과 광계 II(photosystem II)의 광수확 엽록소-단백질 복합체(LHCP II)의 광에너지 분배 기작과의 관계를 구명코저 LHCP II의 인산화에 따른 형광 quenching과 광량별 인산화 정도, 그리고 단백질 조성을 조사하였다. 인삼은 DCMU의 존재 하에서 photosystem II의 형광발생량이 양지식물인 콩에 비해 많았으며, 인간화에 따른 형광 quenching율도 현저히 높았다. 또한, 강광(25k1ux 이상)에서 인삼은 인산화에 따른 형광 quenching율이 콩에 비해서 2배정도 높다는 사실을 확인하였다. 엽록소-단백질 복합체(CP-complex)의 조성비율 및 LHCP II를 구성하고 있는 단백질의 앙과 수적인 면에서 비교식물과 큰 차이를 나타내었는데, 24~29kD 범위에서 인삼은 25, 26, 27kD의 major 밴드와 24, 25.3, 28.3kD의 minor밴드로 구성되어 있었으며 광량에 의존적으로 인산화가 증가하는 인삼의 LHCP II 단백질은 24kD 이었다.

• 동국한의학연구소논문집
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• 제9권
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• pp.1-23
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• 2000

Osteoporosis is a common disorder characterized by reduced bone mineral density, deterioration of the microarchitecture of bone tissue and increased risk of fracture. The aim of treatment of osteoporosis is to maintain and, ideally, to restore bone strength safely. In recent years the role of polypeptide growth factors in bone metabolism has begun to appear. It has been proposed that alterations in the expression or production of growth factor can modulate the proliferation and activity of bone forming cells. Thus, the role of structurally diverse peptides for the management and diagnosis of osteoporosis has attracted the attention of many investigators. This paper reviews numerous findings concerning the use of polypeptides, hormones, and growth factors, for the management of osteoporosis. Many of the compounds mentioned here are experimental prototypes of new therapeutic classes. Though it is unlikely that some of the compounds may ever be used clinically, development of safe and efficacious agents in each class will define the future course of therapy for osteoporosis.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권1호
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• pp.55-59
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• 2001

The vitellin of the red flour beetled Tribolium castaneum Herbst was purified and characterized. The vitellin of T. castaneum was purified by the FPLC techniques, anion exchange chromatography and gel permeation chromatography. In native-polyacrylamide gel electrophoresis, vitellin of T. castaneum was detected as a single band. This native vitellin has molecular weight of 440 kDa. The vitellin of T. castaneum is composed of three polypeptides, designated Vnl (178 kDa), Vn2 (168 kDa) and Vn3 (52 kDa) in SDS-polyacrylamide gel electrophoresis. Three subunits of vitellin were presented in the female adult hemolymph and egg extracts, but not observed in the male. These three polypeptides gradually decreased during embryogenesis. Polyclonal antiserum raised against purified vitellin reacted with the three polypeptides, Vnl, Vn2 and Vn3. Antisera raised against Vn1 and Vn2 cross-reacted with the two large subunits, Vnl and Vn2, respectively. Another subunits Vn3, however, was not cross-reacted with these two antisera. Also, antiserum raised against Vn3 did not cross-react with the Vn1 and Vn2.

• KSBB Journal
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• 제25권2호
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• pp.199-204
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• 2010

Drag-tag으로 사용될 반복단위 단백질을 생물학적인 방법을 통해 생산함으로써 수용액 내에서 DNA 분리가 가능함을 확인하였다. 서로 다른 크기를 갖는 두 종류의 반복단위 단백질을 디자인하였고, 이를 발현시킨 뒤 정제하였다. 정제된 반복단위 단백질에 형광 dye를 포함하고 있는 100 base의 DNA를 연결하였고, 이 연결 물질을 모세관 내부가 수용액으로 충진된 microchip 상에서 전기영동 하였다. 그 결과 생물학적으로 생산된 반복단위 단백질이 SNP 분석과 같은 빠르고 효율적인 DNA 분석에 적합한 후보물질로 사용될 수 있음을 확인하였다.

• Toxicological Research
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• 제6권1호
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• pp.99-107
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• 1990

Dopamine beta-hydroxylase (DBH) is a neurotransmitter biosynthetic enzyme which catalyzes the conversion of dopamine to norepinephrine. Although structural studies of the mature form of this enzyme have been extensive, the culmination of these finding had been hightly controversial and contradictory. In this study, biochemical approaches were taken to characterize the structure of mature DBH. Soluble bovine DBH was purified from aderenal medulla. Three bands of 69 kDa, 72 kDa and 75 kDa which were physically separable and similar in structure were observed by SDS-PAGE. Furthermore, gas phase sequence analysis revealed that the 72 kDa band consists of two polypeptides which are present at equimolar concentrations and differed in that one had three extra amino acids at the N-terminus. Taken together, the soluble form of DBH exist in at least four forms, three identified by SDS-PAGE, one of which consists of two polypeptides as identified by N-terminal sequence analysis. The significance of these forms and their possible biosynthetic mechanisms are discussed.