• 한국환경성돌연변이발암원학회:학술대회논문집
• /
• 한국환경성돌연변이발암원학회 2002년도 Current Trends in Toxicological Sciences
• /
• pp.79-79
• /
• 2002

• 한국독성학회:학술대회논문집
• /
• 한국독성학회 2002년도 Current Trends in Toxicological Sciences
• /
• pp.79-79
• /
• 2002

Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants, and many of their toxic effects, including their immunotoxicities, are mediated by the activation of aryl hydrocarbon receptor (AhR). We previously reported that Aroclor 1254, one of the most widely used PCB mixtures, increased DNA fragmentation in mouse spleen cells, suggesting that apoptosis was correlated with the immunotoxicity of PCB (Yoo et al., 1997).(omitted)

• Journal of Yeungnam Medical Science
• /
• 제3권1호
• /
• pp.103-110
• /
• 1986

Polychlorinated biphenyl(PCB)에 유도된 rat liver cytochrome $P_{450LMII}$을 Balb/c mouse에 주사하여 면역된 spleen cells과 $SP_2$ myeloma cell을 polyethylene glycol(PEG 3500)으로 세포융합 시켜 얻은 fused cell을 계속 배양하여 cloning을 반복하고 ELISA로 확인하여 monoclonal antibody(Mab)를 생산하는 hybrid cell을 얻었으며 mouse 복강내에 hybrid cell(${\times}10^7$)을 주사하여 ascites를 모아 cellulose ion exchange chromatography로 Mab을 정제하였으며 $I^{125}$로 label 시킨 Mab는 $CP_{450LMII}$ 항원과 hybridization시켜 binding을 관찰하였으며 SDS-polyacrylamide 전기영동에서 분자량 55,000 및 110,000인 두 개의 band를 관찰하였다.

• 한국동물학회:학술대회논문집
• /
• 한국동물학회 2000년도 한국생물과학협회 학술발표대회
• /
• pp.66.1-66
• /
• 2000

No Abstract, See Full Text

• 한국환경과학회지
• /
• 제4권3호
• /
• pp.97-97
• /
• 1995

PCBs have been measured using GC-ECD, GC-MS, GC-ELCD, HPLC, TLC, NMR and Immunoassay. The analysis of PCBs using GC-ECD include the peak pattern method as none derivatization and the Perchlorination method as derivatization. This study was conducted to establish the perchlorination method with Sbcls from PCBs to decachlorinated biphenyl(DCB). The aroclor 1242 of PCBs was chlorinated and then, converted into the DCB which showed a single peak in GC-ECD chromatogram. The detection limit of DCB was 2pg. The quantification detection concentration of PCBs extracted with soxhlet was 0.5ng/g in the soil. PCBs were not detected in the suburban soil, but 174ng/g in the soil of industrial complex. Mean PCBs concentration of Shinchun stream at Kumho river and Jinchun stream at Nakdong river was calculated average 낙ngjg in 각e sediment. PCBs concentration in the sediment of Kumho river near 2-7km from conjunction with Nakdong river was average 154ng/g. PCBs concentration in the sediment of Nakdong river near conjunction with Kumho river was average 159ng/g.

• 한국환경과학회지
• /
• 제4권3호
• /
• pp.249-258
• /
• 1995

PCBs have been measured using GC-ECD, GC-MS, GC-ELCD, HPLC, TLC, NMR and Immunoassay. The analysis of PCBs using GC-ECD include the peak pattern method as none derivatization and the Perchlorination method as derivatization. This study was conducted to establish the perchlorination method with Sbcls from PCBs to decachlorinated biphenyl(DCB). The aroclor 1242 of PCBs was chlorinated and then, converted into the DCB which showed a single peak in GC-ECD chromatogram. The detection limit of DCB was 2pg. The quantification detection concentration of PCBs extracted with soxhlet was 0.5ng/g in the soil. PCBs were not detected in the suburban soil, but 174ng/g in the soil of industrial complex. Mean PCBs concentration of Shinchun stream at Kumho river and Jinchun stream at Nakdong river was calculated average 낙ngjg in 각e sediment. PCBs concentration in the sediment of Kumho river near 2-7km from conjunction with Nakdong river was average 154ng/g. PCBs concentration in the sediment of Nakdong river near conjunction with Kumho river was average 159ng/g.

• Environmental Analysis Health and Toxicology
• /
• 제3권3_4호
• /
• pp.39-51
• /
• 1988

Effects of ${\alpha}$-tocopherol and perilla oil on the toxicity of polychlorinated biphenyls (PCB) in male rat were studied. Rats were fed ad libitum for 6 weeks with the animal diet which contains PCB 30 ppm and 100 ppm. Perilla oil (0.5 g/kg body weight) and ${\alpha}$-tocopherol (30 mg/kg body weight) were administered intraperitoneally twice a week for 6 weeks. Rats fed with PCB showed enlargement of liver and spleen, increase in aspartate aminotransferase, alkaline phosphatase, sereum lipid and cytochrome P 450 and decrease in body weight and glutathione. When perilla oil was administered to rats fed with PCB increase in aspartate aminotransferase, alkaline phosphatase, serum lipid and cytochrome P45O and decrease in body weight and glutathione were significantly augmented, compared to rats fed with PCB alone. This means that perilla oil potentiates the toxicity of PCB. On the other hand when ${\alpha}$-tocopherol was administered to rats fed with PCB increase in aspartate aminotransferase, alkaline phosphatase, serum lipid and cytochrome P45O and decrease in body weight and glutathione were signigicantly reduced, compared to rats fed with PCB alone. This means that u-tocopherol reduces the toxicity of PCB. From the above results, it may be concluded that PCB is metabolized by microsomal mixed function oxidase and the metabolite causes the toxicity and microsomal glutathione plays a role of protection on the toxicity of PCB.

• ALGAE
• /
• 제27권1호
• /
• pp.63-70
• /
• 2012

Algae are sensitive to a wide range of pollutants, and are effective bioindicators in ecotoxicity assessments. Here, we evaluated the sub-lethal sensitivity of the marine dinoflagellate Cochlodinium polykrikoides upon exposure to copper (Cu), lead (Pb), bisphenol A (BPA), and Aroclor 1016 (polychlorinated biphenyl, PCB). Toxic effects were assessed by observations of the reduction in cell counts and chlorophyll a levels after exposure to each toxicant. C. polykrikoides displayed dose-dependent, sigmoidal responses when exposed to the tested chemicals. $EC_{50}$-72 h values for Cu, Pb, BPA, and PCB were 12.74, 46.70, 68.15, and $1.07mg\;L^{-1}$, respectively. PCB, which is an endocrine-disrupting chemical, was the most sensitive, proving its toxic effect on the dinoflagellate. This study provides baseline data on the toxic effects of commonly used heavy metals and endocrine-disrupting chemicals to a marine dinoflagellate.

• 한국미생물생명공학회소식지:생물산업
• /
• 제11권3호
• /
• pp.15-20
• /
• 1998

• 한국대기환경학회:학술대회논문집
• /
• 한국대기환경학회 2001년도 추계학술대회 논문집
• /
• pp.159-160
• /
• 2001

반휘발성 유기화합물(Semivolatile organic compounds ; 이하 SOCs)은 높은 증기압과 낮은 반응성 때문에 지구 전역에 폭넓게 존재하고 있다. SOCs중 Polychlorinatd Biphenyls(이하 PCBs)는 난분해성 및 친지질성에 특성으로 환경중에서 순환하기 때문에 최근들어 많은 관심의 대상이 되고 있다. 이들 대부분은 태초의 환경과 모든 matrix(예, 토양, 식물, 대기, 수계 등)에서 발견되어 지고 있다. (중략)