• Title/Summary/Keyword: pollen grain

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Pollen-Mediated Gene Flow between Glufosinate Ammonium-Tolerant GM and Non-GM Rice

  • Lee, Seung-Yeob;Kim, Min-Soo;Kim, Hyo-Jin;Ahn, Jeong-Ho;Baek, So-Hyeon;Shin, Woon-Chul;Kim, Hyun-Soon
    • Journal of Plant Biotechnology
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    • v.34 no.1
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    • pp.47-53
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    • 2007
  • To assess the risk of genetically modified (GM) rice on the agricultural ecosystem, agronomic characteristics, pollen longevity and outcrossing rate between GM (Iksan 483 and Milyang 204) and non-GM (their wild types and female parents) varieties were investigated using the bar gene as a tracer marker in paddy field. The agronomic characteristics of two GM rice were similar to their female-parents (non-GM rice) except heading date and 1,000 grain weight of Iksan 483, and they did not show a difference by the introgression of the bar gene as the genetic traits of rice varieties. Pollen viability was more than 90% just after shedding, and it was rapidly decreased below 50% at 5 minutes after shedding both GM and non-GM varieties. The Pollen longevity was lost after 30 minutes of anthesis. When the distance of gene flow from GM to non-GM rice detected to 6 m from the edge of GM rice plant, the maximum distance of pollen dispersal was 4.5m and 3.9m in Iksan 483 and Milyang 204, respectively, and that was increased in order of west, south, east, and north to the dominant wind direction, west-south. Mean outcrossing rate was very low as 0.003 and 0.001% within 1.5 m from the edge of Iksan 483 and Milyang 204, and the GM hybrids by the pollen dispersal did not detected over 4.5 m from the edge of GM rice plant. The results may help to establish the strategy which reduce the risk of pollen-mediated gene flow between GM and non-GM rice.

Pollen Tube Elongation and Fruit Set of Asian Pear (Pyrus pyrifolia) upon Mechanical Damage on the Style (동양배 암술의 기계적 손상에 따른 화분관 신장과 착과)

  • Kang, Sam-Seok;Kim, Yoon-Kyeong;Choi, Jang-Jeon;Cho, Kwang-Sik;Hwang, Hae-Sung;Jeong, Sang-Bouk;Lee, Hee-Jae
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.942-947
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    • 2010
  • In the present study, pollen tube growth and fruit set in Asian pear ($Pyrus$ $pyrifolia$) were examined upon the mechanical damage on the stigma and style following the artificial pollination. When the upper middle part of style was cut off at 48 h after the artificial pollination, pollen tube grew to the middle of the style and the subsequent pollen tube elongation and fruit set normally occurred. When the style was cut off before the pollen tube reached the middle of the style, however, no pollen tube elongation and fruit set occurred. With the pollen tube elongation through the style, callose plugs at the basal part of the pollen tube were formed to the direction of the elongation at regular intervals. When the upper part of pollen tube attached pollen grain was cut off, however, callose plugs were formed defectively in the pollen tube, and the pollen tube opened and lost their turgor pressure. These facts might be the reason for the ceased or delayed pollen tube elongation and ultimately the failure of the fertilization.

Similarty Relationship and Intraspecific Variation in Pollen Morphology of Korean Subgenus Lepidobalanus E$_{NDL}$.(Genus Quercus L.) (한국산 참나무아속 화분형태의 유사관계 및 종내변이에 관한 연구)

  • 김계환;김상용
    • Journal of Korea Foresty Energy
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    • v.19 no.1
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    • pp.38-48
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    • 2000
  • This study was initiated to investigate similarity and intraspecific variation in pollen morphology of korean subgenus. lepidobalanus Endl, of the genus Quercus L. The parameters measured were polar axis length (PL), equatorial width (EW), colpus length (CL) and copus width (CW), and PE (PL/EW) retios were calculated. The cluster analysis based on these variables of this result showed that the similarity between Q. acutissima Carruther and Q. variabilis Blume was the highest. Wide variation in the pollen grain size of Quercus dentata Thunb. ex Murray might imply, the existence of polyploid and/or aneuploid forms. Studies of ploidy levels within species are recommended for Lepidobalanus species.

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A Palynological Study of the Korean Oldenlandia L. (Rubiaceae) (한국산 백운풀속(꼭두서니과)의 화분학적 연구)

  • Nam, Bo Mi;Chung, Gyu Young
    • Korean Journal of Plant Taxonomy
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    • v.41 no.2
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    • pp.108-112
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    • 2011
  • The pollen of 5 taxa on Korean Oldenlandia L. was investigated by LM and SEM. The pollen grains were monads, $20.7-36.0{\mu}$m in polar length, with an equatorial diameter of $21.0-38.4{\mu}m$. The pollen shapes were oblate-spheroidal or spherical (P/E ratio 0.93-1.00). The apertures numbered 3-7 in number, colporate in shape, with a colpus length of $4.2-20.4{\mu}m$ and a colpus width of $2.6-3.4{\mu}m$. The sexine was $0.9-2.4{\mu}m$ in terms of its thickness, the surface pattern was reticulate, scabrate or neither. Based on these results, the size of the pollen grain, the number of apertures, and the surface pattern of the sexine were all useful for an interspecific classification. The genus Neanotis, established via the pollen characteristics, was thought to be reviewed in its entity regarding the continuous variation of generic characteristics.

Enhanced Extraction of Bioactive Compounds from Bee Pollen by Wet-grinding Technology (벌 화분에서 습식 나노화 공정에 의한 유효성분의 추출)

  • Choi, Yun-Sik;Suh, Hwa-Jin;Chung, Il Kyung
    • Journal of Life Science
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    • v.26 no.6
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    • pp.651-656
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    • 2016
  • Bee pollen is produced by honeybees and is considered one of the most balanced and nourishing nutritional supplements available. Historically, bee pollen has been prescribed for its healing properties and consumed for its high-energy supply. Recent research has provided evidence that bee pollen has diverse biological activities, such as anti-oxidant, anti-inflammatory, anti-bacterial, and even anti-cancer effects. However, the outer membrane of the pollen grain, exine, is highly resistant to most acidic solutions, high pressure, and even digestive enzymes, and the resulting low bioavailability limits its nutritional and clinical applications. This study applied a wet-grinding method to destroy the exine effectively, and it then examined the pollen's enhanced biological activity. First, microscopic observations provided strong evidence that wet grinding destroyed the exine time-dependently. In addition, the content of polyphenols, well-known ingredients of bee pollen and used as internal standards for the quality control of commercial pollen preparations, increased up to 11-fold with wet grinding. Further, the anti-oxidant activity demonstrated on the ABTS anti-oxidant assay, as well as the DPPH radical scavenging assay, was also dramatically increased. Together, the results presented here support a new technology by which bee pollen can be used as a resource for medical, nutritional, and cosmetic applications.

A Study on the Pollen Morphology of Endemic Sapindales in Korea (한국산(韓國産) 무환자나무목(目) 화분(花粉)의 형태학적(形態學的) 연구(硏究))

  • Kim, Kae Kwan
    • Journal of Korean Society of Forest Science
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    • v.55 no.1
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    • pp.1-21
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    • 1982
  • The pollen morphology of 2 forms, 6 varieties, 50 species, 15 genera, 11 families of Korean Sapindales was studied. The keys were provided for general and families of Korean Sapindales. General pollen morphology was diversed and taxonomically significant differences were obtained in shape, aperture, type and size, sculpture pattern and exine thickness of pollen grains. Pollen grains of Korean Sapindales could be grouped into four types and five subtypes. One of five sub-types pollen grains of Acer could be ground into four under subtypes: ginnala type, barbinerve type, psseudo-sieboldianum type and negundo type. Pollen morphology of Empetraceae, Buxaceae and Balsaminaceae appeared to have very special features in the equatorial view and aperature type compare with pollen grain of the other families of Korean Sapindales.

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Varietal Differences of Low Temperature Response at Booting Stage in Rice (벼 수잉기의 저온처리에 의한 품종간 반응)

  • 김기식;김재록;윤경민
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.1
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    • pp.106-112
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    • 1989
  • To investigate varietal differences of anther size, pollen shape, pollen fertility and other agronomic characters under low temperature condition. twenty rice varieties (5 Tongil and 15 Japonica type) were treated at 19$^{\circ}C$ water temperature from booting to heading stage. There was varietal difference of low temperature response which was indicated by the delayed heading, the shortening of culm and panicle length. and the decrease of grain fertility and yield potential resulted from cooling treatment at booting stage. The varieties with low fertility damaged by the low temperature treatment at booting stage showed low pollen density per anther. small or abnormal anthers and pollen, and many sterile pollens. Anther length was correlated significantly with the number of pollens per anther and pollen fertility.

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Quality Breeding Outcome and Outlook in Coarse Grain Crops (잡곡의 품질개량 육종 성과와 전망)

  • Choi Byung Han
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1998.09a
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    • pp.22-34
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    • 1998
  • Coarse grain crops including maize, sorghum, buckwheat, fox-tail millet, pearl millet, proso millet and barnyard millet have been used as health food, feed and industrial materials in Korea for a long time. Korean ancestors thought and treated them as the very important good crops for human health and the crops have served as a dish made with all the grains, particularly in January 15 of the lunar month in korea because the grains make the five viscera of heart, liver, spleen, lungs and kidneys and the six entrails of gall bladder, stomach, small and large intestines, the paunch, the bladder, and the bowels strong and build healthy body. Thus, the objectives of the paper were to review and summarize the results obtained from the quality breeding and functional researches worldwide on nutrition, utilization and medical action of the coarse grain crops. Maize grain, fresh ear and green fodder yields have increased since 1960s in Korea. Agronomic traits improvements also occurred for cold tolerance, disease and insect resistance, resistance to barrenness, resistance to loding, pollen production, grain and seed yields, and eating quality. For buckwheat, improved summer buckwheat varieties produced more rutin for vegetable and grain than autumn varieties in Korea

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Studies on the Functioanl Significance of Pollen Surface Characters (화분표면형질의 기능적 의의에 관한 연구)

  • 이상태
    • Journal of Plant Biology
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    • v.22 no.1_2
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    • pp.45-51
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    • 1979
  • From 32 angiosperm species, pollen surface characters such as sculpture pattern, roughness, oil abundance and stickiness were subjectively scored, and compared with size and weight of the grains, and pollination mechanisms. There was a linear relationship between size and weight of the grains. Animal-pollinated grains showed significantly higher roughness, oil abundance and stickiness than wind-pollinated grains. In the former, the grain size was highly correlated with roughness and oil abundance. No such relationship was found in the latter. It was suggested, therefore, that the large the animal-pollinated grains, the greater is the stickiness required, where the stickiness depends on the surface soil abundance and roughness. No functional significance of the sculpture pattern could not be detected in this study.

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Nutrition, Utilization and Productivity of Pearl Millet Hybrids Developed in Korea (진주조의 영양과 이용 및 생산성)

  • Keun-Yong Park;Rae-Kyung Park;Byeong-Han Choi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.1
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    • pp.103-114
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    • 1994
  • Pearl millet is a $C_4$ plant and summer crop originated from west Africa, and the sixth most important cereal in the world and the most widely cultivated millet in the semi-arid tropics as a major staple food crop. Its grain of higher quality protein is used to make unleavened bread chapatis and prepared as gruel, dumplings, couscous and beer. It is also used as animal feed and forage in both temperate and tropical regions because it has a capability to grow well not only in the fertile soil, but also in the poor and dry soil. Most of the current breeding procedures used in pearl millet are aimed at maximum exploitation of hybrid vigor for both grain and forage yields in Korea. Pearl millet is ideally suited for exploitation of heterosis using cytoplasmic male sterile lines as seed parent, and fertile inbred lines and open-pollinated cultivars as pollen parent. Pearl millet hybrids developed in Korea produced 3 to 7 tons of grain and 100 to 150 tons of green fodder per hectare.

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