• Korean Journal of Human Ecology
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• v.14 no.5
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• pp.833-846
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• 2005

The purpose of this study is to find out the various impressions that tone-on-tone coloration of shirts and ties gives. This experiment was based on the $2\times4\times4\times2$ factorial designs; colors (purple and green), shirt tones (vivid, light, dull, and dark), tie tones (vivid, light, dull, and dark) and perceivers' genders (male and female). The materials in the experiment developed for this study were composed of various stimuli and the response scales for each stimulus. The stimuli were 32 upper body photographs, which were color printed by CAD system (4D-box program). We unified those colors of shirts and ties, and then made shirt and tie tone different. 27 bi-polar adjectives, each of which was graded into seven in its degree, were used to evaluate the impression. The subjects of this research were 192 male and 192 female college students in Gyeongnam province including Jinju City. The data was analyzed by using SPSS program. Analyzing methods were one-way ANOVA and LSD test. The items of the adjectives were classified into 5 impression dimensions; potency, activeness, attractiveness, visibility and tenderness. In conclusion the impression through matching shirts and ties could be varied by the colors and the tones of shirts and ties. This study can be used as the basic color data in the males' clothes market which gradually pursuits high-quality, personality, and variety.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.41-41
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• 2001

Chromatin configuration and microtubule assembly were determined in porcine maturing and activated oocytes following intracytoplasmic sperm injection. Microtubule localization was confirmed using a mouse monoclonal antibody to $\alpha$-tubulin and detected using a fluorescent labeled goat anti-mouse secondary antibody. DNA was stained with propidium iodide. The image of microtubules and chromatin was captured using laser scanning confocal microscope. In germinal vesicle stage oocyte, sperm chromatin remained condensation and sperm derived microtubules were not observed at 8 to 12 h after sperm injection. At 24 h after injection, the sperm nucleus developed to the metaphase chromatin along the metaphase structure of female nucleus. In some metaphase I stage oocytes, sperm chromatin decondensed at 8 h to 12 h after injection, sperm aster was seen soon after sperm injection. At 24 h after sperm injection into metaphase I stage oocyte, male chromatin developed to the metaphase chromatin while female chromatin extruded first polar body and formed the metaphase chromatin. At 12 to 15 h after sperm injection into preactivated oocytes, condensed sperm nucleus was located in close proximity of female pronucleus. However, the condensed nucleus did not fuse with female pronucleus. In preactivated ocytes, injected sperm remained condensation, a few sperm organized small microtubular aster. Instead, maternal derived microtubules were organized near the female chromatin, which seem to move condensed male chromatin near to the female pronucleus. These results suggest that sperm nuclear decondensing activity and nucleation activity of centrosome during fertilization are cell cycle dependent. In absence of male functional centrosome, female origin centrosome takes over the role of microtubule nucleation for nuclear movement.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.18-18
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• 2001

Despite of importance of integrated events of nucleus and microtubule remodeling in nuclear transferred embryos with somatic cells, little information is available on this subject. In this study we configured chromatin and microtubule organization following somatic cell nuclear transfer in pre- and non-activated bovine oocytes in order to clearify nuclear remodeling process and to demonstrate centrosome inheritance during nuclear transfer. The cumulus-oocyte complexes were collected from slaughterhouse and were matured in vitro for 20 h in TCM 199 supplemented hormone. Matured bovine oocytes were enucleated by aspirating the frist polar body and metaphase chromatin using a beveled pipette. Bovine fibroblast cells were fused into enucleated oocyte by electrical stimulation. Reconstructed oocytes were activated with ionomycine and 6-dimethylaminopurin, and then cultured in CRlaa medium. The organization of nuclear and microtubules were observed using laser-scanning confocal microscopy. At 1 hour after fusion, microtubule aster was seen near the transferred nucleus in most oocytes regardless activation condition. While most of fibroblast nuclei remodeled to premature chromosome condensation (PCC) and to the two masses of chromosome in non-activated oocytes, a few number of fibloblasts went to PCC and multiple pronuclear like structures in activated oocytes. Microtubular spindle was seen around condensed chromosome. Gamma-tubulin was detected in the vicinity of condensed chromosome, suggesting this is a transient spindle. The spindle seperated nucleus into two masses of chromatin which developed to the pronuclear like structures. Two pronuclear like structures were than apposed by microtubular aster and formed one syngamy like nuclear structure at 15 h following nuclear transfer. At 17 to 18 h after fusion, two centrosomes were seen near the nucleus, which nucleates micrtubules for two cell cleavage. While 31% of reconstructed oocytes in non-activated condition developed to morulae and blastocysts, a few reconstructed oocytes in pre-activated condition developed to the blastocyst. These results suggested introduction of foreign centrosome during nuclear transfer, which appeared to give an important role for somatic cell nuclear reprogramming.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.6
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• pp.2124-2128
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• 2010

In this study, we determined effects of the mitogen-activated protein kinase (MAPK) inhibitor, U0126 on meiotic maturation, microtubule organization and actin filament assembly in the porcine oocyte. The phosphorylated MAPK was first detected at 12 h after the initiation of maturation cultures, fully activated at 24h, and remained until metaphase II. Treatment of germinal vesicle (GV) stage oocytes with $20{\mu}M$ U0126 completely blocked MAPK phosphorylation, but germinal vesicle breakdown (GVBD) was normally proceeded. However, the oocytes didn‘t progress to the metaphase I. The inhibition of MAPK resulted in abnormal spindles. In oocytes treated with U0126 after GVBD, polar body extrusion was normal, but the organization of the metaphase plate and chromosome segregation were abnormal. In conclusion, MAPK activity plays an important regulatory role in GV chromatin configuration and meiotic progress in porcine oocyte maturation.

• Clinical and Experimental Reproductive Medicine
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• v.20 no.2
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• pp.157-160
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• 1993

The human follicular f1uids(F.F.) may be considered to contribute the maturation of the oocytes on the in vitro culture. To investigate the effects of epidermal growth factor (E.G.F.), which is present in mature and immature follicular fluids, we had experiments of mouse oocytes maturation in vitro. The endpoints assayed were rated as percentage of oocytes undergoing germinal vesicle breakdown(G.V.B.D.) and polar body(P.B.) formation at 12 hours after in vitro culture. The rates of G.B.B.D. were 87% in mature F.F. 68% in immature F.F. and 78% in Ham's F-10 medium respectively. And overall the mature F.F. seem to stimulate on in vitro oocyte maturation compared with either immature F.F. or Ham's F-10 medium. As the concentration of addition of E.G.F. in immature F.F., the rates of G.V.B.D. and P.B. formation were 82 %, 23% in addition with 2 ng/ml while 84%, 32% in addition with 4 ng/ml respectivly. And at the concentration of addition of E.G.F. in Ham's F-10 media as well, the rates of G.V.B.D. and P.B. formation were 84%, 40% and 82%, 44% in addition with each 2ng, 4ng. AccordinglY there was no influence on the oocytes maturation at the addition of E.G.F. to each immature F.F. and Ham's F-10 medium. In conclusion, the E.G.F. is not able to induce oocyte maturation independent of it's effects in immature F.F. and Ham's F-10 media.

• The Korean Journal of Malacology
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• v.29 no.2
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• pp.97-103
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• 2013

In the present study, we monitored the early development of Saccostrea kegakia subtropical oyster species distributing on rocky intertidal off the northern Jeju Island using scanning electron microscope (SEM). The female oyster collected in early August, 2012 were fully mature exhibiting relatively small eggs ($46.5{\pm}1.4{\mu}m$ in diameter) in the gonad, while testis of the mature male oysters were filled with fully developed sperms of 36.9 ${\mu}m$ in length. The fertilized eggs developed into 2-cell stage with polar body after 1 hr 20 min of fertilization, then followed by Morula stage (3 hr 20 min), Blastula stage (4 hr 50 min), Gastrula stage (7 hr), and trochophore larvae stage (9 hr 30 min). The observed early development of S. kegaki in this study was similar the early development of other oysters, although size of the fertilized eggs were somewhat smaller.

• Ocean and Polar Research
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• v.35 no.4
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• pp.323-349
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• 2013

The mud shrimp Upogebia major (Upogebiidae: Decapoda: Crustacea) is a common species on muddy and sandy mud tidal flats in the west coast of Korea. They reside in Y-shaped burrows that can extend up to more than 2 meters below the sediment surface. They feed on suspended detritus carried into their burrow by the beating of their pleopods and captured by their hairy first two pairs of thoracic legs. Mud shrimp burrows provide a habitat for a variety of small organisms such as crabs, shrimps, polychaetes, and mollusks. Ovigerous females are observed from December to May. Females deposit eggs only once per breeding season. They start hatching in March and the pelagic larvae of first zoea appear in March and April, followed by benthic settlement in May. Growth over the first year is rapid, and females deposit their first eggs in the third breeding season, 31 months after their settlement. Adult shrimps live for 4~5 years. Depth of the burrow increases with body length. The deep burrows provide refuge from predators and physical stress, allowing the shrimps to survive for a long time. The mud shrimps supply oxygen-rich water to their deep burrows, and exert a great influence on the structure and metabolism of the tidal flat benthic community. However, recently this type of mud shrimp has posed a serious threat to the Korean clam industry along the west coast of Korea. The extensive burrowing shrimp populations suddenly invaded the tidal flats from 2010 where the clams (Ruditapes philippinarum) are raised. As a consequence, clam production has decreased by about 10% over the past three years in some Korean clam beds. Therefore, the objective of this study is to review the biology of this mud shrimp in order to seek solutions to control the burrowing of these shrimps.

• Clinical and Experimental Reproductive Medicine
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• v.24 no.1
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• pp.51-56
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• 1997

Recently, through the development of the primer extension preamplification(PEP) method which amplifies the whole genome, simultaneous multiple DNA analysis has become possible. Whole genome from each single cell can be amplified using 15 base oligonucleotide random primer. The greatest advantage of PEP-PCR is the ability to investigate several loci simultaneously and confirm results by analysing multiple aliquots for each locus. This technique led to the development of preimplantation genetic disease diagnosis using blastomere from early embryo, sperm, polar body and oocyte. In this study, we applied PEP-PCR in 20 cases of single amniocyte and 20 cases of single chorionic villus cell for the clinical application of the prenatal and preimplantational genetic diagnosis. We analysed 7 gene loci simultaneously which are 46, 47 exons related to dystrophin gene, two VNTR (variable number tandem repeat) markers using 5'dysIII, 3'CA related to dystrophin gene and DYZ1, DYZ3, DYS14 regions on chromosome Y. In all the tests, 97.5% of PEP-PCR amplifications with single cells were successful. We obtained 38/40 (95%) accuracy in gender determination through chromosome analysis comparison. Therefore, these results have significant implications for a sperm or oocyte analysis and prenatal or preimplantational genetic diagnosis.

• Clinical and Experimental Reproductive Medicine
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• v.42 no.4
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• pp.156-162
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• 2015

Objective: To investigate fertilization and embryo quality of dysmorphic mature oocytes with specific morphological abnormalities obtained from intracytoplasmic sperm injection (ICSI). Methods: The fertilization rate (FR) and embryo quality were compared among 58 dysmorphic and 42 normal form oocytes (control 1) obtained from 35 consecutive ICSI cycles, each of which yielded at least one dysmorphic mature oocyte, performed over a period of 5 years. The FR and embryo quality of 441 normal form oocytes from another 119 ICSI cycles that did not involve dysmorphic oocytes served as control 2. Dysmorphic oocytes were classified as having a dark cytoplasm, cytoplasmic granularity, cytoplasmic vacuoles, refractile bodies in the cytoplasm, smooth endoplasmic reticulum in the cytoplasm, an oval shape, an abnormal zona pellucida, a large perivitelline space, debris in the perivitelline space, or an abnormal polar body (PB). Results: The overall FR was significantly lower in dysmorphic oocytes than in normal form oocytes in both the control 1 and control 2 groups. However, embryo quality in the dysmorphic oocyte group and the normal form oocyte groups at day 3 was similar. The FR and embryo quality were similar in the oocyte groups with a single abnormality and multiple abnormalities. Specific abnormalities related with a higher percentage of top-quality embryos were dark cytoplasm (66.7%), abnormal PB (50%), and cytoplasmic vacuoles (25%). Conclusion: The fertilization potential of dysmorphic oocytes in our study was lower, but their subsequent embryonic development and embryo quality was relatively good. We were able to define several specific abnormalities related with good or poor embryo quality.

• Ocean and Polar Research
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• v.25 no.4
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• pp.493-501
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• 2003

Performance of a laboratory scale closed seawater recirculating aquaculture system was evaluated. Twenty-kg of korean rockfish (130 fish) with an average body weight of 153.8g was stocked. Over a 107-day culture period, fish reached final density of $51.7kg/m^3$ (initial density, $33.3kg/m^3$) on the basis of the culture tank volume. On a daily basis, added water amounted to 3.4% of the total water volume in the system. Total ammonia nitrogen (TAN) concentrations were below 1mg/l and nitrite nitrogen $(NO_2-N)$ concentrations were within the range of 1-3mg/l on most sampling days. TAN was removed from bead and sand filters and it was removed or produced in the sedimentation basin. Basically, $NO_2-N$ was removed in the bead and sand filters, while it was either removed or produced in the sedimentation basin. Nitrate nitrogen $(NO_3-N)$ was produced in the bead filters and removed from the sand filter and sedimentation basin. The foam fractionator performed well in the recirculating system. The maximal daily removal values for total suspended solids (755) and protein were 10.9g and 1.4g, respectively. Whole water quality parameters were within the levels commonly recommended for fish culture on most of the sampling days. However, further studies are needed to evaluate the commercial feasibility of this system because of the smallscale system used in present experiment. At least, the present study still provides some basic information for further studies of this kind of system.