• KSBB Journal
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• 제31권1호
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• pp.46-51
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• 2016

Recently paper based diagnostic kits have drawn great interest in the point-of-care testing market (POCT). The paper based detection systems provide inexpensive, rapid and safe analyses for disease markers and/or pathogens. Vitamin C (i.e., ascorbic acid) regulates body's immune system as an antioxidant agent. Humans, however, do not have enough amounts of enzymes involved in the synthesis of vitamin C that it is required to be obtained from their diets (e.g., beverages and/or supplements). Here, we have prepared a paper based kit to detect the concentration of Vitamin C presented in commercially available beverages. The evaluation provides the fast, simple and accurate results for detecting Vitamin C in the prepared paper based kit.

• Journal of Electrochemical Science and Technology
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• 제11권3호
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• pp.310-317
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• 2020

A development of disposable strip-type galactose sensor for point-of-care testing (POCT) was studied, which was constructed using screen-printed carbon electrodes. Galactose levels were determined by the redox reaction of galactose oxidase in the presence of potassium ferricyanide as an electron transfer mediator in a small sample volume (i.e., less than 1 µL). The optimal performance of biosensor was systematically designated by varying applied potential, operating pH, mediator concentration, and amount of enzyme on the electrode. The sensor system was identified as a highly active for the galactose measurement in terms of the sensitivity (slope = 4.76 ± 0.05 nA/µM) with high sensor-to-sensor reproducibility, the linearity (R² = 0.9915 in galactose concentration range from 0 to 400 µM), and response time (t_95% = <17 s). A lower applied potential (i.e., 0.25 V vs. Ag/AgCl) allowed to minimize interference from readily oxidizable metabolites such as ascorbic acid, acetaminophen, uric acid, and acetoacetic acid. The proposed galactose sensor represents a promising system with advantage for use in POCT.

• 한국기술혁신학회:학술대회논문집
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• 한국기술혁신학회 2014년도 추계 학술대회 논문집
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• pp.799-803
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• 2014

• 대한디지털의료영상학회논문지
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• 제10권2호
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• pp.33-37
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• 2008

To keep the online medical records available to anyone without constraint of time and space, introducing EMR (Electronic medical record), which is a clinical support management system. The purpose of this study is to develop interface standard of clinical test device. Integration and sharing of medical information is faced with enormous obstacles because medical organizations and associated companies are separately developing the interface. I hope that multi-function management system with workstation concept is operated to efficiently transmit clinical device result data based on this study. Transfer of precise medical result data available for decision making will improve quality of health care service.

• 한국임상수의학회지
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• 제37권1호
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• pp.23-27
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• 2020

Between May and November 2018, babesiosis was examined in 162 bloods samples obtained to an animal hospital in Jeju island for anemia and medical examination. Sixty-two of 162 (38.3%) were positive by PCR. The ultra fast real-time PCR test with blood directly analyzed without DNA extraction showed the same results. Accurate diagnosis, treatment and prognosis of babesiosis should be combined with clinical symptoms, blood tests, the babesia antibody test, and the PCR antigen test. Ultra fast real-time PCR, with these tests, is expected to be a point-of-care testing (POCT) for easy, fast and accurate diagnosis of babesiosis in the veterinary clinic.

• Genomics & Informatics
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• 제19권3호
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• pp.34.1-34.6
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• 2021

Digital PCR (dPCR) is the third-generation PCR that enables real-time absolute quantification without reference materials. Recently, global diagnosis companies have developed new dPCR equipment. In line with the development, the Lab On An Array (LOAA) dPCR analyzer (Optolane) was launched last year. The LOAA dPCR is a semiconductor chip-based separation PCR type equipment. The LOAA dPCR includes Micro Electro Mechanical System that can be injected by partitioning the target gene into 56 to 20,000 wells. The amount of target gene per wells is digitized to 0 or 1 as the number of well gradually increases to 20,000 wells because its principle follows Poisson distribution, which allows the LOAA dPCR to perform precise absolute quantification. LOAA determined region of interest first prior to dPCR operation. To exclude invalid wells for the quantification, the LOAA dPCR has applied various filtering methods using brightness, slope, baseline, and noise filters. As the coronavirus disease 2019 has now spread around the world, needs for diagnostic equipment of point of care testing (POCT) are increasing. The LOAA dPCR is expected to be suitable for POCT diagnosis due to its compact size and high accuracy. Here, we describe the quantitative principle of the LOAA dPCR and suggest that it can be applied to various fields.

• 농촌의학ㆍ지역보건
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• 제47권4호
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• pp.220-228
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• 2022

119 구급대원들이 사용하는 현장 검사 장비 중 하나인 자가 혈당측정기는 결과 값이 정확해야 환자에게 적절한 처치를 제공할 수 있다. 연구 최초 조사 시점 이후 현재까지도 119 구급대원 현장업무 지침에 현장 검사 장비의 작동 유무, 수량 등을 확인하는 것은 있으나, 장비의 점검에 수량, 작동 여부 등은 있으나 본 연구에서 분석한 정도관리 내용은 없다. 현장검사 장비의 질 관리가 되지 않고 있다는 것은 환자에 대한 부정확한 평가와 이로 인한 불필요한 처치와 과소, 과대처치 또는 환자 상태가 악화될 가능성을 높일 수 있다. 본 연구를 통해, 앞으로 119 구급대원의 혈당측정기를 포함한 모든 현장검사 장비의 사용실태조사가 이루어져야 하고, 119 구급대 현장 업무 지침에 현장 검사 장비 정도관리를 추가하여야 하겠다. 또한, 현장 검사 장비가 관련 지침에 따라서 적절한 질 관리가 이루어져야 하고, 지역에서 관련 업무 전문가나 응급의료지도를 담당하고 있는 지도의사에게 관리되는 것이 합당하겠다.

• 세라미스트
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• 제21권3호
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• pp.293-301
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• 2018

Paper-based analytical devices (${\mu}PAD$) are highly advantageous for portable diagnostic systems owing to their low costs and ease of use. ${\mu}PADs$ are considered as the best candidates for realizing the World Health Organization (WHO) ASSURED criteria: affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable to end users. However, they have several limitations such as low sensitivity and accuracy. This article reports a mini review for a micro-fluidic paper-based analytical devices (${\mu}PAD$), especially for addressing low sensitivity and accuracy issues.

• 한국콘텐츠학회논문지
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• 제14권3호
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• pp.52-58
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• 2014

현장검사는 환자가 있는 즉석에서 검사하여 결과를 알 수 있으며 신속하고 경제적으로 시행할 수 있는 장점이 있어 널리 이용되고 있다. 특히 면역크로마토그래피법을 이용한 검사 항목은 편리하게 쓰이고 있으나 오류가 발생하여 재확인 할 경우, 판독선이 시간이 경과됨에 따라 재확인이 불가능한 단점이 있고 반응이 약한 경우 판독자에 따라 오류가 생길 수 있다. 이에 검사에 쓰이는 다양한 현장검사용 스트립의 판독이 가능하고 화상을 저장할 수 있는 기능을 가진 멀티 스트립 리더기를 개발하였고, 요 임신검사가 의뢰된 검체를 대상으로 개발된 멀티 스트립 리더기를 평가한 결과 100% 일치된 결과를 보였다. 본 연구에서 개발된 멀티 스트립 리더기는 실제 임상에서 편리하고 경제적으로 사용될 수 있을 것으로 생각된다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2007년도 춘계학술대회B
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• pp.2781-2784
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• 2007

Early detection of a disease is important to tackle treatment issues in a better manner. Several diagnostic techniques are in use, these days; for such purpose and tremendous research is going on to develop newer and newer methods. However, more work is required to be done to develop cheap and reliable early detection techniques. Micro-fluidic chips are also playing key role to deliver new devices for better health care. The present study focuses on a review of recent developments in the interrogation of different techniques and present state-of-the-art of microfluidic sensor for better, quick, easy, rapid, early, inexpensive and portable POCT (Point of Care testing device) device for a particular study, in this case, bone disease called osteoporosis. Some simulations of the microchip are also made to enable feasibility of the development of a blood-chip-based system. The proposed device will assist in early detection of diseases in an effective and successful manner.