• Journal of Korean Society of Forest Science
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• v.87 no.3
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• pp.383-390
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• 1998

The purpose of this study was to evaluate the effect of new materials with forest topsoil for hydroseeding measures that is environmentally and ecologically stable revegetation measures on rock cut-slopes. Field hydroseeding experiment was used with a completely randomized design at highway rock cut-slopes in April, 1997. Results obtained in this study were summarized as follows: Particle size distribution of 3 mixed-soil materials that consisted of forest topsoil, decomposed granite soil, compost, sludge, and bottom ash did not show a significant difference. As appending the amount of forest topsoil, soil bulk density was increased. Soil hardness was slightly increased in early period, and then decreased with the flourishing of plants. The number of individuals increased, more than $3,000seedlings/m^2$, after 1 month, and it was decreased as time passes because of competition between the seeded species and the naturally emerged species. In addition to the seeded species, seeding plot has more than 6 species (Rubus crataegifolius, Eleusine indica, Erigeron canadensis, Lycopersicon esculentum var. cerasiforme, etc.) per $m^2$ were naturally appeared in the first year. From the viewpoint of species diversity promotion, the capability of using forest topsoil as seed bank sources was high. In order to apply in the field, the investigation and analysis of topsoil availability (quantity and quality of seed source, soil texture and organic composition) should be carried out before-hand.

• Journal of Korean Society of Forest Science
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• v.69 no.1
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• pp.28-35
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• 1985

To estimate the aboveground biomass of Pinus rigida and Pinus rigida ${\times}$ taeda 22-year-old plantations, the experimental plots of $200m^2$ in size located in Kwangju of Jeonlanam-do were selected. Nine sample trees selected at each plot taking account of DBH distribution were felled and the diagram of oven-dry weight distribution of stem, branch, and needle for each 1m segment was constructed. The logarithmic regression equations between dry weight of each component (stems, branches, and needles) and the variable of $(DBH)^2{\cdot}H$ were obtained. The aboveground standing crops was estimated to be as much as 71.61 and 142.32 tons of dry matter per hectare in P. rigida and P. rigida ${\times}$ taeda stand respectively. The net production was estimated as 10.81 and 10.46 t/ha/yr and the net assimilation rate 1.32 and 1.00 kg/kg/yr in P. rigida and P. rigida ${\times}$ taeda stand respectively. And the efficiency of needles to produce stem was 0.97 and 0.81 kg/kg/yr in same order.

• Journal of Life Science
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• v.16 no.6
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• pp.949-954
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• 2006

Soybean [Glycine max (L.) Merr.] is an important crop, accounting for 48% of the world market in oil crops. Improvement of the quality and quantity of soybean seed constituents is one of the most important objectives in soybean breeding. Protein content and seed size are important properties to determine the quality of tofu and soy sprouts respectively. The objective of this study was to identify quantitative trait loci (QTLs) that control seed weight, protein and oil content in soybean. The 117 $F_{2:10}$ recombinant inbred lines (RlL) developed from a cross of 'Keunolkong' and 'Shinpaldalkong' were used. Narrow-sense heritability estimates based on a plot mean on seed weight, protein and oil content were 0.8, 0.78 and 0.71, respectively. Four independent QTLs for seed weight were identified from linkage group (LG) F, I and K. Five QTL for protein content were located on LG D1b, E, H, I and L. Oil content was related with six QTLs located on LG D1b, E, G, I, J and N. Protein and oil content have three common QTLs on LG D1b, E and I. Thus, we identified major loci improving soybean seed quality.

• Journal of Life Science
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• v.24 no.1
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• pp.1-7
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• 2014

Eukaryotic gene expression is an important process, which is initiated by several transcription factors and RNA polymerases that occupy the promoter region of genomic DNA. Although there are many experiments to identify the promoter region in a gene, it is time and labor consuming to finalize it. In this study, we utilized bioinformatic programs, including Ensembl, NCBI, and CpG plots, to identify the cloning promoter region in SETDB1 genomic DNA. We performed PCR amplification to obtain the SETDB1 promoter on an approximately 2 kb region upstream from the TSS named SETDB1-P1. The PCR product was ligated with TA cloning vectors, and we confirmed the insert size using restriction enzyme digestion. Sequentially, the insert was subcloned into a pGL3-luc vector to produce pGL3-SETDB1- P1-luc and then confirmed by DNA sequencing. We also obtained a fragmented PCR product called P2 and P3 and performed a luciferase assay using pGL3-SETDB1-P1-luc transfection. We found that several anticancer drugs, including taxol, 4-FU, and doxorubicin, decreased the promoter activity of SETDB1. We obtained consistent data on the regulation of SETDB1 gene expression after anticancer drug treatment using Western blot analysis and RT-PCR. Our results suggest that promoter cloning of the human SETDB1 gene utilizing bioinformatics is a very useful and timesaving approach to study gene expression.

• Korean Journal of Environment and Ecology
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• v.18 no.1
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• pp.7-17
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• 2004

The objective of this study was to find eco-revegetation measures recycling remnant root-stock of native species which comes to be disused. The monitoring plot was established in Nov.2001 at Honam University's affliated farm that is located in Wolya-Myeon, Hampyeong-Gun, Jeollanam-Do. The plots were monitored 2 times each in Oct.2002 and Sep.2003. Sprout and survival rate of the root-stock including the effect of trunk length, antiseptic treatment, planting season and number of planted were surveyed. The results of the study were as follows Ten tree species including Zelkova serrata among twenty tree species showed outstanding sprout and survival rate (over 90 percent). The growth rate was better in the longer trunk length root-stocks (35cm) than shorter ones (10cm). There was a difference in the size of the root ball when digging the root-stocks. It showed better condition when the root ball sizes were 3 times wide and 5 times deep of the root-stocks or 5 times wide and 5 times deep. Antiseptic treatment on the cuts of the trunk of root-stocks makes a difference in the growth rate. The survival rate of the root-stock showed highest value (100％) when it was transplanted in late autumn (November). And the next best survival rate was reached when it was in early or late spring (March and May)

• Korean Journal of Environment and Ecology
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• v.9 no.1
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• pp.15-29
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• 1995

To investigate plant community structure of Uiryung valley, Harujae valley, Kugi valley, Songchu valley, Tobongsan valley in Bukhansan National Park, each thirty eight, forty, twenty six. twenty eight, thirty six plots (plot size 100$m^2$) were set up and surveyed. The surveyed data were integrated and classification by TWINSPAN and DCA ordination techniques were applied to the study area. The plant community were divided into nine groups in seventy nine plots by TWINBPAN and the dividing groups were Carpinus laxiflora community(I), Quercus mongolica community(II), Pinus densiflora community(III), Q. mongolica-P. densiflora community(IV) Q. mongolica-Q. serrata- Prunus sargentii community(V), Q. mongolica Q. variabilis community(Ⅵ), Robinia pseudoacacia-Q. serrata community(Ⅶ). P. rigida-Q. serrata community(Ⅷ), Q. mongolica- Q. variabilis- Styrax obassia community(Ⅸ). So the successional trends of tree species at the natural forest seem to be from P. densiflora to Q. mongolica and at the artificial forest seem to be from R. pseudoacacia, P. rigida to Q. acutissima, Fraxinus rhynchophylla in canopy layer And in the subtree and shrub layer, it wag supposed that from Deotzia prunifolia, Rhus trichocarpa. Rhododendron yedoense var. poukhasleilse, Euonymus oxyphyllous to Magnolia sieboldii, Acer psuedo-sieboldianum, Lindera obtusiloba. In comparing successional trends with past study, it was postulated that successtion is not progressed by human disturbance and soil acidification by the air pollution.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.19 no.1
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• pp.20-30
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• 1993

Liquid Chromatographic behavior of Pd(II) in Isonitosoethylacetoacetate imine IEAA-NR: R=H, CH3, C2H5, n-C3H7, n-C4H9, C6H5-CH2) Chelates were investigated by reversed phase high performance 1iquid chromatography on Micropak MCH-5 Column using Methanol /water as mobile phase. The optimum condition for the separation of Pd-Isonitrosoethylacetoacetate imine chelates were examined with respect to the flow rate, mobile phase strength. It was found that Pd(IEAA-NR)2 chelates were eluted in an acceptable range of the capacity factor value (0 $\leq$ log k' $\leq$ 1), The dependence of the logarithm of capacity factor(k') on the volume fraction of water in mixture with in the binary mobile phase was examined. Also, the dependence of k'on the liquid-liquid extraction distribution constant in methanol-water / n-alkane extraction system was on system was invert tigated for Pd(IEAA-NR)2. Both kinds of dependence are linear, which suggests that the retention of the electroneutral metal chelates be largely due to the solvophobic effect.

• Korean Journal of Plant Resources
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• v.24 no.4
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• pp.370-378
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• 2011

To address infra-specific relationships of Sedum kamtschaticum Fisch., and to provide the fundamental information for developing new horticultural variations, we analyzed the morphology of individuals in four natural populations (Mt. Gwangdeok, Mt. Samyeong, Mt. Yonghwa, Tongyeong) and investigated the vegetations of these area. Based on 50 morphological characters the principle component analysis (PCA) has been performed. Principle component axis 1, 2, and 3 explain 22.9%, 14.2%, and 7.4% of total variations, respectively. Dot plot of OTUs in PC2 by PC1 area showed that the areas of four populations are completely overlapped. The result of PCA and the statistics of each character indicate that all of morphological characters are overlapped in these four populations. The maximum deviations are found in the characters related in the size and shape of the leaf. In the vegetation analyses, eighteen community plots that we investigated were grouped into 10 subcommunities: subcomm. Boehmeria spicata, subcomm. Artemisia stolonifera, subcomm. Artemisia keiskeana, subcomm. Impatiens nolitangere, subcomm. Crepidiastrum chelidoniifolium, subcomm. Urtica thunbergiana, subcomm. Artemisia gmelini, subcomm. Commelina communis, subcomm. Erigeron annuus-Artemisia princeps, and typical subcommunity.

• Journal of Life Science
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• v.26 no.8
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• pp.881-886
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• 2016

Cephalanthera erecta (Thunb. ex, Murray) is an herbaceous and a member of the genus Cephalanthera in the family Orchidaceae. The species is an herbaceous and has reputed Chinese medicinal value. It has been investigated the population density and spatial distribution of this species at Mt. Ahop in Korea during 2015. The spatial pattern of C. erecta was analyzed according to several patchiness indexes, population uniformity or aggregation under different sizes of plots by dispersion indices, and spatial autocorrelation. The mean crowding (M*) and patchiness index (PAI) showed positive values except one small plot (2 m × 2 m). Most natural individuals of C. erecta for plots were not uniformly distributed in the forest community. The small plots (2 m × 2 m, to 8 m × 16 m) of C. erecta were uniformly distributed in the forest community and large plots (16 m × 16 m and 16 m × 32 m) were aggregately distributed. Significant aggregations by Moran's I of C. erecta were partially observed within IV classes (12 m). Dissimilarity among pairs of individuals could found by more than 18.0 m. In conclusion, the geographic distribution of C. erecta is not even with varying degrees of size of plots and human activities give rise to density effects in the plots at Mt. Ahop in Korea.

• The Korean Journal of Ecology
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• v.28 no.2
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• pp.99-103
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• 2005

Actual vegetation map drown with the scale 1 to 100 and Alnus japonica population in Ja-neup and Woong-neup of the Mujechi moor were described in order to monitor long-termly and preserve permanently, where is a very rare Molinietea moor and a legally protected area. A total of 3036 plots of 5m $\times$ 5m were surveyed during summer 1996. Thiessen polygons of 1491 alder trees were derived from the plot data. Actual vegetation map was illustrated by 6 cover types such as needle spike-rush type, moor-grass type, alder-moor type, eulalia type, oak forest type, and exposed site. Molinia grasses native to the moor and Miscanthus grasses alien to the moor are reciprocally dominant. The area of Molinia grasslands was rapidly in decline and alder population size was dramatically in increase in the moor, particularly in Woong-neup. In Molinietea moor preservation more attention should be focused on the regulation of a nutrient rich soil from forest road and fire.