• 한국경제지리학회지
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• 제7권2호
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• pp.117-136
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• 2004

본 논문의 목적은 접경지역 연구에 대한 문헌을 검토하고, 특히 여기서 발견되는 주요한 이론적 모델과 국내외 사례를 비판적으로 고찰하여 경기도 접경지역에 적합한 분석 틀 구축에 대한 시사점을 얻는데 있다. 접경지역 연구가 오래 전부터 행해진 유럽과 미국 및 최근의 동남아시아나 국내 사례에 대한 연구 결과, 다음과 같은 몇 가지 특징적인 사실들이 발견되었다. 첫째, 국경 및 접경지역과 관련된 용어상의 혼란이 존재한다. 둘째, 접경지역 연구에서 개발된 모형들은 초기에는 경계가 가지는 장벽효과를 설명하기 위하여 개발되었으나, 점차 접경지역이 폐쇄적인 공간에서 개방적인 공간으로 변화함에 따라 이에 걸맞는 모델들이 개발되고 있다. 셋째, 접경지역을 다양하게 유형화려는 시도가 있었으며, 이것들은 궁극적으로 접경지역의 발전 방향을 제시하고 있다. 넷째, 해외사례를 검토한 결과 접경지역이 더 이상 고립되거나 변방에 머무르지 않고, 강력한 지원과 지역협력을 통해서 성장이 기대되는 지역으로 변화하고 있다.

• International Journal of Oral Biology
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• 제30권1호
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• pp.1-8
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• 2005

The elderly suffer from an impaired immune function being obvious in a higher susceptibility to infections. Although the inflammatory cells are the major immunomodulatory cells, fibroblasts also secrete a variety of inflammatory cytokines and chemokines. Therefore periodontal tissue aging might playa role in development and progress of periodontitis. In this study, we investigated the effect of in vitro periodontal ligament cellular aging on the inflammatory cytokines, chemokines, and matrix metalloprotease(MMP)-2 expression induced by lipopolysaccharide(LPS) treatment. Three different cell populations were used; passages 4-5, 14-15, and 24-25 (at passage 27, more than 90% cells were replicative senescent). LPS increased the expression of interleukin(IL)-1${\beta}$, IL-6, and tumor necrosis factor-${\alpha}$, IL-8, RANTES, and MMP-2. However, the order of induction folds were passages 14-15 > 4-5 > 24-25. While the expression level of Toll-like receptor(TLR) 4 decreased according to the increase in passage number, the level of TLR2 was highest at passages 14-15 and then decreased at passages 24-25. While the spontaneous expression of IL-8 decreased according to the increase in passage number, that of RANTES and proMMP-2 increased according to the increase in passage number. These results suggest that the aging of periodontal ligament fibroblasts differentially affect the role as immunomodulatory cells in response to periodontopathic bacteria and therefore might be another risk factor of periodontitis progression.

• 한국의상디자인학회지
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• 제10권3호
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• pp.127-136
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• 2008

The main focus of the study resides in antecedents of price acceptability. Levels of acceptable price may be related to the consumers' perception on reasonable or expected price. Price acceptability is known to have several psychological antecedents. One of the antecedents to price acceptability reported by prior researches is price-quality inference, a tendency to correlate high price to excellence in quality. In addition, price-conscious consumers are likely to show lower level of price acceptability level. Another well-known antecedent is sale proneness. Sales-prone consumers may relate price of apparel products to product quality information. Moreover, it was reported that involved consumers should be more concerned with the products to its price and thus should have higher levels of price acceptability. A conceptual model with price consciousness, sale proneness and product involvement as the exogenous variable, price-quality inference and price acceptability as the endogenous variable was developed for the empirical study. Measures of research variables were developed based on previous studies. Questionuaires from 298 respondents were analyzed for the study. The average age of respondents was 27. About 60% of the respondents were married and about 65% of them had college degrees. Empirical results supported all of the hypothesized relationships. Price consciousness had significant negative influence on price-quality inference and price acceptability. Sale proneness significantly influenced price-quality inference, while apparel involvement had significant impact on price-quality inference and price acceptability. Price-quality affected price acceptability significantly. This study generated a framework to help scholars understand antecedents of price acceptability of apparel products. Price has been shown to playa dual role in consumer's perceptions, either positively or negatively. Price consciousness played a negative role, and product involvement had a positive role in evoking higher level of price acceptability. This study also suggests additional source of positive, yet indirect role of price, sale proneness. This study also affirmed the importance of price-quality inference in arousing higher level of price acceptability.

• 대한가정학회지
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• 제44권10호
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• pp.21-34
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• 2006

The purpose of this study is to define the roles of the central, district, and municipal Healthy Family Support Centers in order to establish their individual identities. Two hundred twenty-five 225 subjects were surveyed with a questionnaire about the role of each Healthy Family Support Center. The subjects of this study were professors, public officials and staff who were in charged of the Healthy Family Support Centers. This study revealed the following results. First, the central Healthy Family Support Center should playa supportive role to develop and propagate a variety of programs which can be used for the clients in the district and municipal Healthy Family Support Centers. Second, roles of the district Healthy Family Support Center are highly recognized not only for their supportive role in activating municipal Healthy Family Centers but also for their publicity role in them. Finally, the most necessary role of the municipal Healthy Family Support Center is to manage family counseling and family education. In order to accomplish the roles of each Healthy Family Support Center, the mutual relationship among central, district, and municipal Healthy Family Support Centers should be horizontal. Moreover, district Healthy Family Support Centers should be promptly established in order to enable for the central Center to play its roles properly.

• 대한의생명과학회지
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• 제14권3호
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• pp.179-186
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• 2008

Matrix metalloproteinases (MMPs), also designated matrixins, hydrolyze components of the extracellular matrix. These proteinases playa central role in many biological processes, such as embryogenesis, normal tissue remodeling, wound healing, and angiogenesis, and in diseases such as atheroma, arthritis, cancer, and tissue ulceration. In previous data, disruption of the actin cytoskeleton by cytochalasin D (CD) inhibited NO-induced apoptosis, dedifferentiation, cyclooxygenase (COX)-2 expression, and prostaglandin $E_2$ production in chondrocytes cultured on plastic or during cartilage explants culture. In this study, we investigated the effects of the actin cytoskeleton architecture on MMP-2 expression and dedifferentiation by CD in rabbit articular chondrocytes. Rabbit articular chondrocytes were prepared from cartilage slices of 2-weeks-old New Zealand white rabbits by enzymatic digestion. CD was used as a disruptor of actin cytoskeleton. In this experiments measuring CD dose response, primary chondrocytes were treated with various concentrations of CD for 24h. The actin disruption was determined by immunostaining. MMP-2 expression levels were determined by immunoblot analysis and Reverse transcriptase-Polymerase chain reaction (RT-PCR) and MMP-2 activity was determined by gelatin zymography. We found that cell morphological change and up-regulation of MMP-2 expression by CD as determined via immunostaining, gelatin zymography and immunoblotting. Moreover, CD induced MMP-2 transcription was detected by RT-PCR. Also, CD-induced type II collagen expression was inhibited by MMP-2 inhibitor I treatment. Our results indicate that CD up-regulated MMP-2 activation causes dedifferentiation of articular chondrocyte.

• BMB Reports
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• 제40권6호
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• pp.853-860
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• 2007

Resistance to anticancer drugs is a major obstacle in the effective treatment of tumors. To understand the mechanisms responsible for multidrug resistance (MDR), a proteomic approach was used to identify proteins that were expressed in different levels by the adriamycinresistant human gastric cancer cell line, SGC7901/ADR, and its parental cell line, SGC7901. Two-dimensional gel electrophoresis (2-DE) and image analysis was used to determine which protein spots were expressed in different levels by the two cell lines. These spots were then partially identified using ESI-Q-TOF mass spectrometry, and the differential expressional levels of the partially identified proteins were then determined by western blot analysis and real-time RT-PCR. Additionally, the association of Nucleophosmin (NPM1), a protein that was highly expressed by SGC7901/ADR, with MDR was analyzed using siRNA. As a result of this study, well-resolved, reproducible 2-DE patterns of SGC7901/ADR and SGC7901 were established, and 16 proteins that may playa role in the development of thermo resistance were identified. Additionally, suppression of NPMl expression was found to enhance adriamycin chemosensitivity in SGC7901/ADR. These results provide a fundamental basis for the elucidation of the molecular mechanism of MDR, which may assist in the treatment of gastric cancer.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
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• pp.79-79
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• 2003

Recently diabetes has been found to be associated with metabolic bone diseases such as osteoporosis. In the present study, attempts have been made-to explore the effect of high glucose in bone formation. Osteoblast-like UMR 106 cells were treated with high glucose (22mM, 33mM, 44mM) for 1 or 2 days. High glucose significantly inhibited proliferation of UMR106 cells in a time- and dose- dependent manner as evidenced by MTT assay. For the evaluation of collagen synthesis, UMR 106 cells were cultured in high glucose media (44mM) for 24 h and the ratio of collagen content to total protein was measured. In addition, gene expression pattern of type I collagen was assessed by RT-PCR. The high concentration of glucose inhibited a collagen synthesis, a marker of bone formation activity. JNK, c- Jun N-terminal Kinase, is known to play an important role in stress-associated cell death. In this regard, we tested to determine whether high glucose has any effect on JNK activity. It has been found that treatment of high glucose induced phosphorylation of JNK. On the other hand, ERK phosphorylation was inhibited by high glucose in a dose-dependent manner. Taken together, Therefore these results indicate that inhibition of proliferation in UMR 106 cells following high glucose is related to JNK/ERK containing signal pathways. This study showed high glucose concentration could alter the bone metabolism leading to defective bone formation, suggesting that high glucose due to diabetes may playa significant role in the development of metabolic bone disease.

• Clinical and Experimental Reproductive Medicine
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• 제10권1호
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• pp.25-37
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• 1983

A group of 180 men who visited Urology Department of Severance hospital, including 115 patients with nongonococcal urethritis (N.G.U.), 27 patients with prostatitis, 13 patients with gonococcal urethritis (G.U.) and 25 healthy medical student controls were investigated for the isolation of Ureaplasma urealyticum (T-strain mycoplasma) from the specimen of ureaplasma discharge, urine and semen. Taylor-Robinson media of T-broth and T-agar was used for the isolation of Ureaplasma urealyticum. To the best of our knowledge, the study on the culture of Ureaplasma urealy ticum was reported for the first time in Korea. The followis g results were obtained: 1. The isolation rate of Ureaplasma urealyticum in nongonococcal urethritis (53.0%) revealed highest of those in the other three groups of prostatitis, gonococcal urethritis and control (40.7%,38.4% and 16.0% respectively). 2. As for the specimens, urethral discharge revelaed higher isolation rate of Ureaplasma urealyticum (54.6%) than first voided urine (50.0%). 3. The more consorts patients had, the higher positive culture rate of Ureaplasma urealyticum were revealed. The isolation rate in case of more than one causal in nongonococcal urethritis (27.8%) revealed much higher than in case of marital only (5.2%), one regular (6.1%) and one causal 03.9%). 4. 2.6% of isolation rate of Ureaplasma urealyticum revealed in patients with nongonococcal urethritis who visited the clinic in later than 4 weeks after the symptoms developed. However, the isolation rate in patients who visited within 4 weeks revealed 50.3%. The lower isolation rate of Ureaplasma in the late treatment seekers might be probably due to the suppression effect against Ureaplama urealyticum from the possible previous self antibiotic treatment. 5. Attachment of Ureaplasma urealyticum mostly to the neck and head portion of the spermatozoa seemed to playa role to affect the motility of sperms.

• 산업경영시스템학회지
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• 제31권1호
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• pp.116-123
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• 2008

Food and Agriculture Organization (FAO) and many countries make an effort to conserve and utilization of animal genetic resources to prepare for our unpredictable future. In order to protect the customer and the producer from the animal diseases and unjust distribution, many country seek to appropriate solution. Among the solutions, RFID technology can be used as a basic technology, since this technology can be applied in the conservancy and utilization of animal genetic resources, object management for improved animal and traceability of animal's distribution flow. There are two main issues in making the efficient RFID environment. The first issue is the standardization of code system for object identification. International Organization for Standard (ISO) published the standard which regulates RFID of animal (ISO 11784 and ISO 11785). Based on these standards, many countries have tried to establish their national standard. In Korea, National Institute of Animal Science (NIAS) playa main role in establishing the standard of object identification code based on ISO 11784. Even though the standard format of object's identification is well established, the RFID system may not be operated well without the standardization of RFID network and related equipment. In Korea, National Internet Development Agency of Korea (NIDA) has proposed the RFID Network at 2006, which can be applied in the different kind of system at each phase. But, the implementation case of this RFID Network does not reported yet, since many company or agency who introduce RFID technology, implemented as an isolated individual system. In our study, we show that RFID network can be utilized for any kind of system at each phase, and propose the improvement point in order to be widely used.

• 대한두경부종양학회지
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• 제18권1호
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• pp.23-29
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• 2002

Mutation of the P53 tumor suppressor gene playa major role in the development of many carcinomas, namely in the colon, breast and bladder, whereas the role played by such mutations in thyroid carcinogenesis remains controversial. Vascular endothelial growth factor (VEGF) induces proliferation of endothelial cells, stimulates angiogenesis, and increases vascular permeability. Increased VEGF expression has been associated with poor clinical outcomes in many malignancies E-cadherin, a calcium-dependent transmembrane glycoprotein, is an adhesion molecule Expression of p53, VEGF and E-cadherin was assessed immunohistochemically in 19 tall columnar variant of papillary carcinoma, 24 common papillary carcinoma and 7 follicular carcinoma. The aim of this study was to evaluate the expression of P53,VEGF and E-cadherin as a potential maker for the prognosis of thyroid carcinomas. The results are as follows: 1) There were no significance in any clinical parameters examined among tall columnar variant of papillary carcinoma, common papillary carcinoma and follicular carcinoma. 2) The expression of P53 demonstrated low in tall columnar variant of papillary carcinoma, common papillary carcinoma and follicular carcinoma, but a significantly high in regional lymph node metastasis. 3) The expression of VEGF demonstrated a significantly high in regional lymph node metastasis than those without metastasis in papillary thyroid carcinoma. 4) The expression of E-cadherin demonstrated less often among papillary carcinomas with lymph node metastasis than in those without metastasis in papillary thyroid carcinoma. In conclusion, it is suggested that VEGF and E-cadherin will be useful for the diagnosis of thyroid carcinoma and serves as a biological marker for thyroid carcinoma lymph node metastasis.