• Title/Summary/Keyword: plasma values

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Antioxidant Status and its Relationship to Plasma Cytokine Levels in Korean Elderly Women Living in Seoul

  • Kim, Mi-Joung;Kim, Jung-Hee
    • Journal of Community Nutrition
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    • v.6 no.2
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    • pp.103-109
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    • 2004
  • Body antioxidant status is an important factor for the prevention of many chronic diseases in the elderly. This study was done to investigate antioxidant status and its relationship to immune response by measuring plasma cytokine (IL-2 and IL-6) levels in elderly women. Subjects were 76 elderly women aged over 60 years, visiting Jangwhi Social Welfare Center of Seongbook-Gu in Seoul. Subjects were divided into 3 groups according to age (< 65, 65 - 74, > 75). Dietary intakes were assessed by semi-quantitative food frequency questionnaires (SFFQ). Plasma vitamin C level was measured by 2,4-dinitrophenylhydrazine method and plasma levels of vitamin E, A and ${\beta}$-carotene were measured by HPLC. Plasma levels of IL-2 and IL-6 were determined with a solid phase sandwich enzyme linked-immuno-sorbent assay (ELISA) using commercial kits. The average intakes of antioxidant vitamins were 96.3mg (137.5% of RDA) for vitamin C and 523.3 ${\mu}$gRE (74.8% of RDA) for vitamin A in elderly women. All of the average plasma levels of antioxidant vitamins were within normal range. However the percentage of the elderly women with deficiency plus marginal values were 7.9% in vitamin C, 9.2% in vitamin A and 7.9% in vitamin E. Plasma levels of IL-2 and IL-6 were 27.1${\pm}$7.1pg/ml and 5.9${\pm}$5.3pg/ml in elderly women. Correlation data showed that plasma IL-2 level was negatively correlated with plasma vitamin C level. In addition, IL-6 level was also negatively correlated with plasma vitamin C, A and E levels, respectively. There was a significant positive correlation between erythrocyte thiobarbituric acid-reactive substance(TB-ARS) level and plasma IL-2 or IL-6 levels. In addition, erythrocyte TBARS level showed a significant positive correlation with plasma total antioxidant status (TAS) level and a significant negative correlation with plasma vitamin C level. Overall results might imply that the decreased levels of antioxidant vitamins result in an increase in oxidative stress and thereby increase cytokine production such as IL-2 and IL-6. However further research is required to elucidate these relationships.

Isolation of Angiotensin Converting Enzyme Inhibitors from Pig Blood (돼지혈액으로부터 항고혈압물질의 분리)

  • Park, Eun-Hee;Song, Kyung-Bin
    • Applied Biological Chemistry
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    • v.40 no.1
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    • pp.39-42
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    • 1997
  • Angiotensin converting enzyme (ACE) inhibitors were isolated and purified from pig blood plasma. Pig blood plasma was obtained after removing blood cells by centrfugation, followed by the addition of anticoagulant to whole pig blood. To precipitate plasma proteins, pig blood plasma was treated with 4% trichloroacetic acid (TCA) as a final concentration. ACE inhibitors were isolated from plasma protein hydrolysates and TCA supernatant, using ultrafiltration, gel permeation chromatography, and reverse-phase high pressure liquid chromatography. ACE inhibitors isolated from plasma hydrolysates and TCA supernatant had $IC_{50}$ values of $23\;{\mu}M$ and $2\;{\mu}M$, pentapeptide, respectively.

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Measurement and assessment of imperfections in plasma cut-welded H-shaped steel columns

  • Arasaratnam, P.;Sivakumaran, K.S.;Rasmussen, Kim J.R.
    • Steel and Composite Structures
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    • v.6 no.6
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    • pp.531-555
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    • 2006
  • H-shaped welded steel column members are fabricated by welding together pre-cut flanges and the web. Modern fabricators are increasingly using plasma-cutting technique instead of traditional flame cutting. Different fabrication techniques result in different degrees of geometric imperfections and residual stresses, which can have considerable influence on the strength of steel columns. This paper presents the experimental investigation based temperature profiles, geometric imperfections, and built-in residual stresses in plasma cut-welded H-shaped steel column members and in similar flame cut-welded H-shaped steel columns. Temperature measurements were taken during and immediately after the cutting operations and the welding operations. The geometric imperfections were established at closely spaced grid locations on the original plates, after cutting plates into plate strips, and after welding plate strips into columns. Geometric imperfections associated with plasma cut element and members were found to be less than those of the corresponding elements and members made by flame cutting. The "Method of Section" technique was used to establish the residual stresses in the plate, plate strip, and in the welded columns. Higher residual stress values were observed in flame cut-welded columns. Models for idealized residual stress distributions for plasma cut and flame cut welded sections have been proposed.

Surface Modification of Polypropylene Fiber by Plasma Discharge (방전처리에 의한 Polypropylene섬유의 표면개질)

  • 허만우;이창재;강인규;한명호;김삼수;임학상
    • Textile Coloration and Finishing
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    • v.11 no.2
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    • pp.27-37
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    • 1999
  • Polypropylene(PP) films were treated with plasma glow discharge to produce peroxy radicals on the surfaces. The peroxy radicals formed on the PP film surfaces were subsequently used for the graft polymerization of acrylic acid and acrylamide in an aqueous solution by heating, respectively. Introduction of acrylic acid and acrylamide on the PP film could be confirmed by the observation of carbonyl and primary amine absorptions based on carboxylic acid and amide, respectively. And introduction of functional group could be confirmed by weight analysis and ESCA. The water contact angle(90$^{\circ}$) of PP film was constant, irrespective of elapsed time, while plasma-treated and functional monomer-grafted PP films were slowly increased with elapsed time, showing the rearrangement of surface polar groups in air condition. The water contact angle$(90^\circ)$ of PP film was decreased by the plasma treatment$(56^\circ)$ and further decreased by the grafting of acrylic acid$(34^\circ)$ and acrylamide$(37^\circ)$, indicating increased hydrophilicity of the modified surfaces. The water contact angle of plasma-treated PP film increased a little as time elapsing. The half-life periods of surface voltage on acrylic acid-(31sec) and acrylamide-grafted PP(42sec) were significantly decreased when compared to those on PP(950sec) and plasma-treated PP film(241sec). In the experiments using acid, basic and disperse dyes, absorbance and $\Delta{E}$ values of functional monomer-grafted PP films were significantly increased than that of oxygen plasma-treated one.

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TREATMENT OF ZINC DEFICIENCY IN SHEEP BY ZINC CONTAINING BOLUSES

  • Khandaker, Z.H.;Telfer, S.B.
    • Asian-Australasian Journal of Animal Sciences
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    • v.3 no.1
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    • pp.53-59
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    • 1990
  • A study was conducted to investigate the release pattern of zinc form the zinc containing boluses and to see whether the released zinc can cure a zinc deficiency in sheep. Three sheep were used in this experiment and were fed a low zinc semi-synthetic diet throughout the experimental period. Each sheep was given a single pre-weighed zinc containing bolus when blood variables showed continuous zinc deficiency. The zinc containing boluses when placed within the reticulo-rumen of zinc deficient sheep, release zinc at the rate of 106.6 mg zinc/day for 39 days. At the end of depletion period there was a reduced feed consumption, plasma zinc concentration, plasma alkaline phosphatase activity and increased plasma zinc binding capacity which were 409 g, 0.18 mg/l, 87 U/l and 88.7% respectively and 521 g, 0.18 mg/l, 142 U/l, and 89.5% respectively before first and second blousing. After the administration of the first and second boluses, the feed consumption, plasma zinc levels and plasma alkaline phosphatase activities rose rapidly and far exceeded the starting values. The zinc binding capacity was reduced to 21.9% due to the administration of the first and second boluses. It is concluded that zinc boluses can be used for curing a zinc deficiency in sheep.

Antibacterial Activity of Dielectric Barrier Discharge Plasma against Main Food-borne Bacteria in Suspensions (유전체장벽방전 플라즈마에 의한 주요식중독세균의 살균 효과)

  • Choi, Man-Seok;Kim, Ji Yoon;Jeon, Eun Bi;Park, Shin Young
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.52 no.6
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    • pp.617-624
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    • 2019
  • Dielectric barrier discharge (DBD) plasma is one of the promising next generation non-thermal technologies for food sterilization. The present study investigated the effects of DBD plasma on the reduction of most common food-borne pathogenic bacteria (Staphylococcus aureus, Bacillus cereus, Vibrio parahaemolyticus, Salmonella enterica) and sanitary indicative bacteria (Escherichia coli) in the suspension (initial inoculum of approx. 9 log CFU/mL). The bacterial counts were significantly (P<0.05) reduced with the increase in the treatment time (1-30 min) of DBD plasma in the suspension. The D-values (time for 90% reduction) of DBD plasma by first-order kinetics for S. aureus, B. cereus, V. parahaemolyticus, S. enterica, and E. coli were 17.76, 19.96, 32.89, 21.55, and 15.24 min, respectively (R2>0.90). These results specifically showed that 30 min of DBD plasma treatment in > 90% reduction of seafood-borne pathogenic and sanitary indicative bacteria. This suspension study may provide the basic data for use in seafood processing and distribution.

Optimization of Plasma Process to Improve Plasma Gas Dissolution Rate using Three-neck Nozzle (3구 노즐을 이용한 플라즈마 가스 용존율 향상을 위한 플라즈마 공정의 최적화)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Science International
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    • v.30 no.5
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    • pp.399-406
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    • 2021
  • The dissolution of ionized gas in dielectric barrier plasma, similar to the principle of ozone generation, is a major performance-affecting factor. In this study, the plasma gas dissolving performance of a gas mixing-circulation plasma process was evaluated using an experimental design methodology. The plasma reaction is a function of four parameters [electric current (X1), gas flow rate (X2), liquid flow rate (X3) and reaction time (X4)] modeled by the Box-Behnken design. RNO (N, N-Dimethyl-4-nitrosoaniline), an indictor of OH radical formation, was evaluated using a quadratic response surface model. The model prediction equation derived for RNO degradation was shown as a second-order polynomial. By pooling the terms with poor explanatory power as error terms and performing ANOVA, results showed high significance, with an adjusted R2 value of 0.9386; this indicate that the model adequately satisfies the polynomial fit. For the RNO degradation, the measured value and the predicted values by the model equation agreed relatively well. The optimum current, gas flow rate, liquid flow rate and reaction time were obtained for the highest desirability for RNO degradation at 0.21 A, 2.65 L/min, 0.75 L/min and 6.5 min, respectively.

Influence of periodontal treatment on the glucose and lipid metabolism in Korean type 2 diabetic patients (한국인 제2형 당뇨병환자에서 치주치료가 당질 및 지질대사에 미치는 영향)

  • Kim, Sung-Heub;Hong, Ji-Youn;Kim, Chang-Sung;Choi, Seong-Ho;Cho, Kyoo-Sung;Chae, Jung-Kiu;Kim, Chong-Kwan
    • The Journal of the Korean dental association
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    • v.47 no.2
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    • pp.90-101
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    • 2009
  • The aim of this study was to appraise the influence of conventional periodontal treatment on metabolic control in Korean type 2 diabetic patients. In addition, their periodontal change was compared with non-diabetic patients. Before and after treatment, it was performed to measure periodontal and metabolic indices in thirteen type 2 diabetic patients. Periodontal indices included plaque index, gingival index, bleeding on probing, probing pocket depth, gingival recession, and clinical attachment level. Metabolic indices included glycated hemoglobin(HbA1c), fasting plasma glucose, fasting plasma insulin, total cholesterol, triglyceride, and HDL-cholesterol. Plaque index, gingival index, bleeding on probing, probing pocket depth, and gingival recession showed significant improvements in the statistics. Diabetic patients showed no statistically significant differences in the changes of periodontal indices compared with non-diabetic patients. HbA1c values decreased in five of the thirteen subjects and fasting plasma glucose levels were reduced in four of the seven subjects after periodontal treatment. All five subjects whom HOMA values were calculated in showed the increases of insulin secretions. The results of this study ascertained the possibility of the better glycemic contol after conventional periodontal treatment in Korean type 2 diabetic patients and diabetes were well healed of their periodontal diseases after the treatment.

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Application of Inactivation Model on Phytophthora Blight Pathogen (Phytophthora capsici) using Plasma Process (플라즈마 공정을 이용한 고추역병균(Phytophthora capsici) 불활성화 모델의 적용)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Science International
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    • v.24 no.11
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    • pp.1393-1404
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    • 2015
  • Ten empirical disinfection models for the plasma process were used to find an optimum model. The variation of model parameters in each model according to the operating conditions (first voltage, second voltage, air flow rate, pH, incubation water concentration) were investigated in order to explain the disinfection model. In this experiment, the DBD (dielectric barrier discharge) plasma reactor was used to inactivate Phytophthora capsici which cause wilt in tomato plantation. Optimum disinfection models were chosen among ten models by the application of statistical SSE (sum of squared error), RMSE (root mean sum of squared error), $r^2$ values on the experimental data using the GInaFiT software in Microsoft Excel. The optimum models were shown as Log-linear+Tail model, Double Weibull model and Biphasic model. Three models were applied to the experimental data according to the variation of the operating conditions. In Log-linear+Tail model, $Log_{10}(N_o)$, $Log_{10}(N_{res})$ and $k_{max}$ values were examined. In Double Weibull model, $Log_{10}(N_o)$, $Log_{10}(N_{res})$, ${\alpha}$, ${\delta}_1$, ${\delta}_2$, p values were calculated and examined. In Biphasic model, $Log_{10}(N_o)$, f, $k_{max1}$ and $k_{max2}$ values were used. The appropriate model parameters for the calculation of optimum operating conditions were $k_{max}$, ${\alpha}$, $k_{max1}$ at each model, respectively.

Effects of Yullmoo (Coix lachryma-jobi var. mayuen stapf.) ext. on Lipidlowering and Serum Glucose in Hyperlipidemic Rat (율무추출액이 비만쥐의 지질강하 및 혈당치에 미치는 영향)

  • Lim Sang-Cheol
    • Korean Journal of Plant Resources
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    • v.19 no.1
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    • pp.126-129
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    • 2006
  • Effects of Coix lachryma-jobi var. mayuen stapf. ext. on lipidlowering and serum glucose were investigated in hyperlipidemic rat. Concentration of glucose and triglyceride in plasma showed a tendence to decrease in Coix lachryma-jobi var. mayuen stapf. ext. groups. However the values of 100mg/kg treatment group showed no significant difference in control group. Plasma total cholesterol and LDL-chol. showed a low values than those of control group. However HDL-chol. showed no significant difference in all treatment groups, Concentration of liver total cho. and triglyceride showed a tendence to decrease in Coix lachryma-jobi var. mayuen stapf. ext. groups. Concentration of plasma and liver TBARS showed a low values in Coix lachryma-jobi var. mayuen stapf. ext. groups. The values of GSH-Px, SOD and CAT activity showed a tendence to increase in the Coix lachryma-jobi var. mayuen stapf. ext. groups.