• Asian-Australasian Journal of Animal Sciences
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• 제13권10호
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• pp.1359-1366
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• 2000

An experiment was carried out to study plasma levels of hormones and metabolites of crossbred Holstein cattle during late pregnancy (28 days pre partum), early lactation (30 days post partum), mid-lactation (120 days post partum) and late lactation (210 days post partum). Two breed types of Holstein $Friesian{\times}Red$ Sindhi (50:50 = 50%HF) and Holstein $Friesian{\times}Red$ Sindhi (87.5:12.5 = 87.5%HF) were divided into four groups of four animals each. Two groups of each breed were fed with either rice straw treated with 5% urea or pangola hay (Digitaria decumbens) as the source of roughage throughout the experiments. There were a substantial increases in the mean levels of total triiodothyronine ($T_3$), insulin and glucagon at the onset of lactation, and maintained in a high levels during lactation advance for all groups of experiments. The mean levels of prolactin and thyroxine ($T_4$) were not significantly different among groups of animals, but the plasma cortisol concentration was slightly higher in both groups of 50%HF in comparison with those of 87.5%HF animals. The mean levels of plasma growth hormone (GH) of both groups of 87.5%HF animals feeding on either hay or urea treated rice straw markedly rose in the early period of lactation and markedly reduced in mid- and late lactation. These changes were accompanied with changes of milk yield. In contrast to 50%HF animals, plasma GH levels were considerably higher in the late pregnant period than in the early period of lactation and it remained constant as its value at the early lactation throughout the experimental period. The high levels of both plasma progesterone and estradiol concentration significantly declined after parturition and remained low through lactating period. The plasma glucose level in the 50%HF animals feeding on either hay or urea treated rice straw was higher than the 87.5%HF animals in all periods of experiments. Changes in plasma FFA levels of both types of crossbred animals were depended on the endocrine status during late pregnancy and lactation. The levels of plasma FFA of 50%HF animals were significantly higher (p<0.05) than those of 87.5%HF animals during late pregnancy. Both plasma ${\beta}$-hydroxybutyrate and lactate concentrations were not affected by feeding on either hay or urea treated rice straw during late pregnancy and lactation. These data demonstrate that there were no differences in the physiological performances in the same crossbred animals fed either hay or urea treated rice straw. The 87.5%HF animal has the genetic potential for a high milk yield and homeorhetic adaptation for mammary function differed from 50%HF animals during periods of lactation. Altering lactation persistency in 87.5%HF is regulated mainly by chronically acting growth hormones through the period of lactation.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3977-3980
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• 2015

Background: To investigate the effects of preemptive analgesia with parecoxib sodium on haemodynamics and plasma stress hormones in surgical patients with thyroid carcinoma. Materials and Methods: Fifty-seven patients with thyroid carcinoma who underwent thyroidectomy selectively in Laiwu Hospital Affiliated to Taishan Medical University and Binzhou People's Hospital were selected and randomly divided into three groups, 19 cases in each group. The control group was intravenously injected 0.9% sodium chloride injection before anesthesia induction; trial group I was intravenously injected with parecoxib sodium 20 min before anesthesia induction; based on trial group I, trial group II was injected with parecoxib sodium again 12 h after surgery. The levels of plasma norepinephrine (NE), cortisol (Cor) and blood glucose before, 12 and 24 h after surgery and changes of haemodynamics before surgery, at the end of surgery and 12, 24 and 48 h after surgery were compared in the three groups. Besides, visual analogue scale (VAS) scores were recorded 4, 8, 12 and 24 h after surgery. Results: 12 and 24 h after surgery, the levels of plasma NE and Cor in three groups rose dramatically (P<0.05 or (P<0.01); The levels of plasma NE and Cor in trial groups I and II were evidently lower than in control group (P<0.05 or P<0.01), and those in trial group II slightly lower than in trial group I. 12 h after surgery, the heart rates (HR) and systolic pressures (SBP) in trial groups I and II increased obviously by comparison to surgery before (P<0.05 or P<0.01), but gradually returned to the preoperative level. HR, SBP and diastolic pressures (DBP) in trial groups I and II at the end of surgery and 12 h after surgery were all lower than in the control group, and significant differences were present (P<0.05 or (P<0.01). At 4, 8, 12 and 24 h after surgery, VAS scores in trial groups I and II were markedly lower than in the control group (P<0.05 or (P<0.01), the scores in trial group II being the lowest. Conclusions: Combined application of parecoxib sodium for preemptive analgesia before anesthesia and after surgery can effectively reduce the levels of plasma stress hormones and improve analgesic effects in surgical patients with thyroid carcinoma, and without conspicuous impact on haemodynamics.

• Asian-Australasian Journal of Animal Sciences
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• 제11권4호
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• pp.368-374
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• 1998

The study was conducted on six multiparous Murrah buffaloes which were earlier artificially induced into lactation. During the experimental period of 15 days, buffaloes were managed in a loose housing system. All the buffaloes were administered a single injection of bromocryptine (@ $100{\mu}g/kg$ body weight) subcutaneously in the neck region at 08:30 A.M., 50 days postpartum (early lactation). Blood samples were collected from four buffaloes for a period of 5 days before the administration of bromocryptine i.e. on days -5, -4, -3, -2, -1, on day of treatment (day 0) and thereafter daily for a period of 9 days i.e 1, 2, 3, 4, 5, 6, 7, 8 and 9 to determine the hormones and blood metabolites. Homogeneous milk samples from all the buffaloes were collected at morning and evening milkings on days coinciding with the days of blood sampling for analysis of milk constituents. Administration of bromocryptine resulted in a significant inhibition of plasma prolactin within 24 hrs of treatment, but the response in all the buffaloes was not uniform. The effect of bromocryptine on plasma prolactin hormone lasted for 1-4 days but Cortisol concentration were not altered. Administration of bromocryptine neither affected blood glucose nor plasma non-esterified fatty acids concentration. Irrespective of level of milk production from different buffaloes, there was no effect of bromocryptine on milk yield which indicated that prolactin is not required for milk secretion during early lactation in buffaloes. Milk constituents like fat, protein and lactose were not affected by bromocryptine may be due to no effect of bromocryptine of milk yield.

• Asian-Australasian Journal of Animal Sciences
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• 제19권1호
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• pp.35-41
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• 2006

The aim of this study was to investigate the effects of three herb supplementations on blood metabolites, hormones, antioxidant activity, immunoglobulin (Ig) G concentration, and ruminal fermentation in steers. Four Holstein steers in a $4{\times}4$ Latin square design received four herb treatments. The treatments consisted of the steers' regular diets with addition of: 1) nothing (control), 2) peppermint, 3) clove, and 4) lemongrass at 5% of the diet (DM basis). Clove supplementation increased the plasma concentration of cholesterol by about 10% (from 79 to 87 mg/dl). Peppermint and lemongrass feeding resulted in an increase in the concentrations of plasma urea nitrogen (from 5.9 to 6.9 and 6.4 mg/dl, respectively). The three herb treatments had no effect on other metabolites and hormones. Steers receiving clove supplementation showed a higher plasma antioxidant activity. The three herb treatments caused lower concentrations of IgG in the blood. Peppermint and lemongrass feedings increased, and clove feeding decreased ruminal concentrations of ammonia. There were no significant differences in VFA concentrations among herbal treatments, except for the decrease in propionate concentration in steers receiving clove treatment. This study suggested that clove feeding changed cholesterol metabolism and increased antioxidant activity in plasma, and feeding of three herbs affected immunity system and ruminal fermentation in steers.

• 한국수산과학회지
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• 제43권5호
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• pp.474-481
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• 2010

The sperm collection time, sex steroid hormones, and gonadal development of protandrous black porgy, Acanthopagrus schlegeli, acclimated in freshwater for more than 2 years were investigated to evaluate its reproductive capability. The gonadal development of black porgy reared in seawater and freshwater could each be classified into four successive stages. For black porgy reared in seawater (BSW) as the control, these were the growing (December to February), mature (February to March), spent (March to June), and degeneration and resting (July to December) stages; for black porgy reared in freshwater (BFW), these were the growing (November to January), mature (January to February), spent (February to May), degeneration and resting (June to November) stages. In both BSW and BFW, the plasma cortisol levels were the highest in March. The plasma testosterone (T) levels of BSW and BFW were the highest in March and February, respectively. The plasma estradiol-$17{\beta}$ ($E_2$) levels did not differ significantly between BSW and BFW. The 11-ketotestosterone (11-KT) levels in the plasma of BSW and BFW were the highest in April. Sperm was collectible from March to June in BSW and from February to May in BFW. The results indicated that the gonadal maturation of BFW was about 1 month faster than that of BSW.

• Child Health Nursing Research
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• 제11권3호
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• pp.301-307
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• 2005

Purpose: To investigate changes in mother's stress, degree of depression and level of milk secretion hormones when the mothers provided massage to their new born babies. Method: The study was conducted with 65 women who had delivered at a convalescence institute in Incheon between Sep 25, 2003 and Feb 10, 2004. The mothers were assigned to a control group or an experimental group. Infants of mothers in the experimental group received massage from their mothers twice a day for 10 days beginning on the 4th day after delivery. The data were processed using $x^2-test$, paired t-test and repeated measures ANOVA using the SAS program. Results: The level of cortisol, a stress hormone and depression were significantly different between the two groups (p=0.01 ;p=0.02). While fur plasma prolactin level, a milk secretion hormone, there was no significant difference(p=0.14). Conclusions: When massage was provided to new-born babies the cortisol level of the mothers decreased significantly compared to the control group which did not receive the massage. As the level of depression of the control group declined as time passed while the experiment group showed no difference and there was no difference in the plasma prolactin level, further study is suggested.

• Asian-Australasian Journal of Animal Sciences
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• 제2권3호
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• pp.147-148
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• 1989

• Journal of Preventive Medicine and Public Health
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• 제43권4호
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• pp.301-308
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• 2010

Objectives: In most DEHP exposure assessment studies, single spot urine sample was used. It could not compare the exposure level among studies. Therefore, we are going to represent the necessity of selection of proper sampling time of spot urine for assessing the environmental DEHP exposure, and the association urinary DEHP metabolites with steroid hormones. Methods: We collected urine and plasma from 25 men. The urine sampling times were at the end of the shift (post-shift) and the next morning before the beginning of the shift (pre-shift). Three metabolites of DEHP {mono(2-ethylhexyl) phthalate [MEHP], mono-(2-ethyl-5-hydroxyhexyl)phthalate [MEHHP], and mono(2-ethyl-5-oxohexyl)phthalate [MEOHP]} in urine were analyzed by HPLC/MS/MS. Plasma luteinzing hormone, follicle stimulating hormone, testosterone, and $17{\beta}$- estradiol were measured at pre-shift using a ELISA kit. A log-transformed creatinine-adjusted urinary MEHP, MEHHP, and MEOHP concentration were compared between the post- and pre-shift. The Pearson’s correlation was calculated to assess the relationships between log-transformed urinary MEHP concentrations in pre-shift urine and hormone levels. Results: The three urinary metabolite concentrations at post-shift were significantly higher than the concentrations in the pre-shift (p<0.0001). The plasma hormones were not significantly correlated with log-transformed creatinine - adjusted DEHP metabolites. Conclusions: To assess the environmental DEHP exposure, it is necessary to select the urine sampling time according to the study object. There were no correlation between the concentration of urinary DEHP metabolites and serum hormone levels.

• 한국수산과학회지
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• 제40권4호
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• pp.226-233
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• 2007

Physiological responses of mullet Mugil haematocheilus to cold shock in winter were investigated. The experimental mullets were initially acclimated at $10.0^{\circ}C$ and then the water temperature was reduced to $-1.2^{\circ}C$ for cold shock experiment. The stress responses was monitored for nearly 50 hours. The parameters monitored include survival rate, plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), glucose (GLU), total protein (TP), electrolytes $(Na^+,\;K^+,\;Cl^-)$, cortisol and thyroid hormones $(TT_4,\;TT_3,\;FT_4\;and\;FT_3)$. With the exception of the TP and electrolytes, most parameters changed significantly during the cold shock. The survival rate did not change from $10^{\circ}C\;to\;-0.6^{\circ}C$, but decreased significantly below $-1.0^{\circ}C$, and was zero at $-1.2^{\circ}C$. The plasma AST and ALT concentrations increased remarkably from $2.5^{\circ}C\;to\;0.5^{\circ}C$ and from $2.5^{\circ}C\;to\;1.5^{\circ}C$, respectively, and then declined rapidly as the temperature decreased to $-1.2^{\circ}C$. The plasma GLU concentration did not change until -0.5'E, and then the concentration increased significantly at $-1.2^{\circ}C$. The plasma cortisol concentration increased remarkably from $2.5^{\circ}C\;to\;-0.5^{\circ}C$, and then declined at $-1.2^{\circ}C$. The plasma thyroid hormones showed two changes during the cold shock. Both plasma 74 concentrations increased remarkably from $2.5^{\circ}C$\;to\;0.5^{\circ}C$, then declined rapidly until $-1.2^{\circ}C$, while both plasma 73 concentrations decreased significantly from $10^{\circ}C\;to\;2.5^{\circ}C$, and then remained significantly lower than the concentration at $10^{\circ}C$.

• Biomolecules & Therapeutics
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• 제22권6호
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• pp.570-576
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• 2014

Studies have demonstrated that electromagnetic waves, as the one of the most important physical factors, may alter cognitive and non-cognitive behaviors, depending on the frequency and energy. Moreover, non-ionizing radiation of low energy waves e.g. very low frequency waves could alter this phenomenon via alterations in neurotransmitters and neurohormones. In this study, short, medium, and long-term exposure to the extremely low frequency electromagnetic field (ELF-EMF) (1 and 5 Hz radiation) on behavioral, hormonal, and metabolic changes in male Wistar rats (250 g) were studied. In addition, changes in plasma concentrations for two main stress hormones, noradrenaline and adrenocorticotropic hormone (ACTH) were evaluated. ELF-EMF exposure did not alter body weight, and food and water intake. Plasma glucose level was increased and decreased in the groups which exposed to the 5 and 1Hz wave, respectively. Plasma ACTH concentration increased in both using frequencies, whereas noradrenaline concentration showed overall reduction. At last, numbers of rearing, sniffing, locomotor activity was increased in group receiving 5 Hz wave over the time. In conclusions, these data showed that the effects of 1 and 5 Hz on the hormonal, metabolic and stress-like behaviors may be different. Moreover, the influence of waves on stress system is depending on time of exposure.