• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.112-112
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2005.11a
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• pp.178-178
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• 2005

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.109-109
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• 2004

• 한국약용작물학회:학술대회논문집
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• 2010.10a
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• pp.491-492
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• 2010

• Proceedings of the Botanical Society of Korea Conference
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• 1998.07a
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• pp.53-60
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• 1998

• Journal of Plant Biotechnology
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• v.48 no.3
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• pp.123-123
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• 2021

• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.42-48
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• 2011

Chloroplast genetic engineering of higher plants offers several unique advantages compared with nuclear genome transformation, such as high levels of transgene expression, a lack of position effect due to site-specific transgene integration by homologous recombination, multigene engineering in a single transformation event and reducing risks of gene flow via pollen due to maternal inheritance. We established a reproducible chloroplast transformation system of potato using a tobacco specific plastid transformation vector, pCtVG (trnI-Prrn-aadA-mgfp-TpsbA-trnA). Stable transgene integration into chloroplast genomes and the homoplasmic state of the transgenome were confirmed by PCR and Southern blot analyses. Northern, immunoblot analysis, and GFP fluorescence imaging revealed high expression and accumulation of GFP in the plastids of potato leaves. This system would provide new opportunities for genetic improvement and mass production of value added foreign proteins in this crop.

• Journal of Plant Biotechnology
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• v.7 no.2
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• pp.113-121
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• 2005

The objective of this study was to identify the major parameters controlling DNA delivery by particle bombardment to immature embryos of Chinese spring wheat (Triticum aestivum L.). Efficiency of DNA (uidA gene) delivery was assessed by transient GUS (${\beta}$-glucuronidase) expression in bombarded tissues. Of the parameters analyzed, acceleration pressure, bombardment distance, chamber vacuum pressure, bombardment times, osmotic conditioning of culture had a remarkable influence on transient gene expression. A bombardment procedure suitable for Chinese spring wheat cultivars was developed which allowed high-efficiency DNA delivery combined with reduced damage to target tissues. The high efficiency made the system practical for wheat genetic transformation research and accelerating wheat breeding programs.

• Korean Journal of Plant Tissue Culture
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• v.28 no.3
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• pp.147-151
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• 2001

Explants of Lactuce sativa cultivar, chungchima, were co-cultivated with Agrobacterium tumefaciences LBA4404, EHA101 strains containing nptll gene and ferritin gene encoding iron storage protein from soybean for transformation. Through initial selection of regenerated explants by culturing on a kanamycin and carbenicillin containing MS medium, multiple shoots were obtained after 2 months of culture. For a complementary step of selection, putative transgenic shoots were transferred to 1/2 MS basal medium supplemented with 100 mg/L kanamycin and 500 mg/L carbenicillin. The selected shoots were tested with PCR analysis using nptll, ferritin specific primers whether ferritin gene was introduced to genome of the plants. These results confirmed that produced the specific PCR bands in the putative transgenic lines. Additionally the Northern blot showed that transcripts of ferritin gene were detected in mature leaf of the transgenic lines. These results suggest that ferritin gene be successfully integrated and transcribed in the putative transgenic lettuce plants.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1996.11a
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• pp.964-968
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• 1996

The demands of size and quality of large steel shaft forgings for ship building, power plant, steel plant, etc. are rapidly increasing, and some of these productions are manufactured from ingot weighing more than 300 tons. For use as rotating components. shafts require toughness, strength and homogeneity, and therefore are produced through a variety of heat treatments. According to the increase of ingot size, micro- and macrosegregation and also mass effect of the product increase. Thus, special care should be paid to the heat treatment of such large shaft forgings. In this paper, the heat treatment of large shaft forgings such as rotor and back-up roll is calculated using the commercial finite element code SYSWELD. Calculated distributions of temperature and phase are compared with experimental data. The continuous cooling transformation diagram, thermal and mechanical properites of each phase are used. The phase proportion, hardness and residual stress during water quenching are discussed.