• Natural Product Sciences
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• 제9권4호
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• pp.299-303
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• 2003

For the screening of inhibitors of sterol biosynthesis from natural products, a simple and rapid assay method was developed using recombinant yeast carrying human lanosterol synthase, main target of this assay method. Sterol biosynthesis inhibition activity was monitored only by the inhibition of growth of the recombinant yeast. By changing the substrate, this assay method can figure out which step is inhibited in the sterol biosynthesis by the test material. With this assay method total 102 plant samples were screened for their inhibitory activity of sterol biosynthesis. Among plant water extracts screened, 11 plant samples showed inhibitory activity on sterol biosynthesis in ergosterol (-) medium. For selection of the specific inhibitory materials, 11 plant samples were reassayed in ergosterol (+) medium. After all 5 plant samples, Abutilon avicennae Gaertn. (stem), Alnus japonica Steud. (stem), Amaranthus mangostanus L. (aerial part), Philadelphus schrenckii Pupr. (leaf) and Pimpinelia brachycarpa Nakai (aerial part), showed specific inhibitory activity.

• Biomolecules & Therapeutics
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• 제8권2호
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• pp.167-170
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• 2000

The present study was conducted to investigate the acute toxicity of plant sterol ester by a single oral dose in Sprague-Dawley rats. Ten males and 10 females aged 5 weeks were randomly assigned to two groups of 5 rats each and were administered by gavage at dose level of 0 or 20 ml/kg body weight. Parameters measured during the 14-day observation period were mortality, clinical signs, body weight changes, and gross findings. No mortality was observed in the present study. Treatment-related clinical signs, such as pasty stool and diarrhea, were observed on the day of treatment and these signs resulted in soiled fur on day 1 after the treatment. However, no clinical signs were observed on days 2-14 after the treatment. There was no significant difference in body weight changes between the control and treatment groups. At necropsy on day 14 after the treatment, no treatment-related gross findings were observed in the treatment group. Based on these results, it was concluded that a single oral dose of plant sterol ester induced pasty stool and diarrhea in Sprague-Dawley rats at dose level of 20 ml/kg and that the lethal doses were considered to be over 20 ml/kg for both sexes.

• 한국식품과학회지
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• 제9권1호
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• pp.41-46
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• 1977

1. 무환자(無患子)나무종자중(種字中)의 유지함량(油脂含量)은 45.7%로서 콩, 참깨, 땅콩에 비해서 많은 편에 속한다. 2. 지방산(脂肪酸)은 Oleic acid(61.6%)와 Gadoleic acid(20.4%)로서 주요성분(主要成分)을 이루고 있고 monoene산(酸)이 전체지방산(全體脂肪酸)의 82%를 차지하고 있는것이 특징적(特徵的)이다. 3. Amino산조성(酸組成)은 Glutamic acid의 함량(含量)이 6.673%로서 가장높고 대체적으로 필수(必須) amino산(酸)인 Isoleucine(1.255%), Leucine(2.145%), Lysine(2.191%), Phenylalanine(1.536%), Threonine(1.171%), Valine(1.772%)등이 함량(含量)이 높았다. 4. Sterol조성(組成)은 4-desmethyl sterol, 4-monomethyl sterol fraction에서 다 같이 일반고등식물중(一般高等植物中) 성분(成分)과 대차(大差)가 없었으며 단지 4-monomethyl sterol fraction에 있는 RRT 1.27(31.5%)의 미동정물질(未同定物質)이 있다는 것이 특이(特異)하였다.

• 생약학회지
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• 제31권1호
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• pp.39-44
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• 2000

A sterol mixture, 3-O-glucosides of these sterols, 6'-O-fattyacyl ester of these sterol glucosides, kaempferol, quercetin and isoquercitrin were isolated from the whole plants of Hypericum ascyron L. The sterols were found to be a mixture of ${\beta}-sitosterol$, campesterol and stigmasterol by GC-MS. The kinds of fatty acids linked at 6'-OH of sterol glucoside ester mixture were shown to be palmitic acid, stearic acid, oleic acid and linoleic acid by GC-MS. Three flavonoids were identified by spectroscopic methods and comparisons of mixed mp and co-TLC with authentic specimens.

• 미생물학회지
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• 제28권4호
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• pp.351-363
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• 1990

A mutant was selected by NTG treatment of Mycobacterium sp. NRRL B-3805, which was capable of degrading plant sterol to androsta-4-ene-3, 17-dione and yields was higher than NRRL B-3805. Also this mutant produced androst-4-ene-3, 17-dione faster than NRRL B-3805. It described the mode of sitosteroidal degradation, and the interrelation between cell membrane and its attachment to substrate during the sterol degradation process by this mutant and it was compared with Mvcobacterium sp. NRRL B-3805.

• Nuclear Engineering and Technology
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• 제2권2호
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• pp.73-84
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• 1970

Solanum andigena의 괴경생성형상과 스테톨의 관계를 구명하기 위하여 방사성 매바론산을 이용하여 대사를 연구하였다. 괴경생성에 단일광조성수도를 요하는 단일식물과 괴경을 생성치 않는 장일식물을 접목 시켰을때 장일식물이 괴경생성 홀몬을 받아 괴경을 생성하는 현상을 스테로이드와 결부시켜 구명할려고한것이다. 이 연구에서 각종 방사성 스테톨이 특수장치한 까스 크로마토 그라피로서 분리 측정되었다. 여기서 분리된 각종 스테톨이 직정 괴경생성홀몬이라는 근거는 찾을 수 없으며, 이들이 괴경의 포대성장에 필요한 인자로서 관여하고 있는 것으로 논의되었다.

• Toxicological Research
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• 제18권1호
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• pp.31-41
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• 2002

The present study was conducted to investigate the potential subacute toxicity of plant sterol esters by a 4-week repeated oral dose in Sprague-Dawley rats. The test article was administered once daily by gavage to rats at dose levels of 0, 1000, 3000, and 9000 mg/kg/day for 4 weeks. During the test period, clinical sign, mortality, body weights, food and water consumption, ophthalmoscopy, urinalysis, hematology, serum biochemistry, gross finding, organ weight, and histopathology were evaluated. A reduction in the body weight was observed in females of the 9000 mg/kg group on day 27 after the initiation of treatment, but not in males of the group. There were no treatment-related effects on mortality, clinical sign, food and water consumption, ophthalmoscopy, urinarlysis, hematology, serum biochemistry, necropsy findings, organ weights, and histopathology in any treatment group. Based on these results, it was concluded that the 4-week repeated oral dose of plant sterol esters resulted in suppressed body weight in female rats at a dose level of 9000 mg/kg/day. In the condition of this study, target organ was not observed and the no-observed-adverse-effect level (NOAEL) was considered to be 9000 mg/kg/day for males and 5000 mg/kg/day for females.

• 생약학회지
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• 제8권4호
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• pp.149-153
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• 1977

Evodia daniellii $H_{EMSLEY}$ (Rutaceae) is cultivated in Korea and used as a folkmedicine for gastric inflammation, extermination of noxious insects, headache and antiinflammatory. The seed oil of this plant also has been used in various diseases, for example, dermatitis, scabies and so forth. Fructus Evodiae seed oil sterols were obtained from the nonsaponifiable matters of ethereal extract of the seed. The composition of sterols have been determined by gas liquid chromatography and thin layer chromatography on the preparative plates. It was noted that ${\beta}-sitosterol$ was the major sterols in the Evodia daniellii $H_{EMSLEY}$ in Korea. The results showed that contents of Evodia daniellii $H_{EMSLEY}$ sterols were campesterol 14.01%, stigmasterol 4.36%, ${\beta}-sitosterol$ 71.26%, ${\delta}^7-sterol$ 9.21% and unknown sterol 1.16% by method of triangulation and campesterol 13.02%, stigmasterol 3.87%, ${\beta}-sitosterol$ 74.34%, ${\delta}^7-sterol$ 8.67% and unknown sterol 1.85% by method of planimetry.

• Applied Biological Chemistry
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• 제21권1호
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• pp.51-57
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• 1978

3종(種)의 가지과(科) 식물중(植物中)의 sterol조성(組成)을 TLC, GLC 및 GLC-MS에 의(依)해서 분석(分析)하여 4-desmethylsterol fraction에서 7종(種), 4-monomethylsterol fraction에서 9종(種), 그리고 4.4-dimethylsterol fraction에서는 8종(種) 모두 24종(種) sterol을 동정(同定)하였고, 이들 sterol들이 식물체내(植物體內)에서 어떤 생합성경로(生合成經路)를 거치는가를 검토(檢討)하였다.

• Applied Biological Chemistry
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• 제42권3호
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• pp.267-270
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• 1999