• The Plant Pathology Journal
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• 제35권1호
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• pp.51-62
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• 2019

A great variable response was observed when PP-3 and PP-J encumbered with 116 populations of root knot nematode (RKN) at two different temperatures ($25{\pm}2^{\circ}C$ and $30{\pm}2^{\circ}C$) and concentrations ($10^4$ and $10^5$ spores/ml). The PCR reaction amplified intergenic region between cytochrome oxidase subunit II gene (COII) and large subunit of rRNA gene (lrRNA) of the mitochondrial genome of different RKN species. The primer C2F3 and 1108 identified M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans.

• 식물병연구
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• 제23권4호
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• pp.348-354
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• 2017

2013년부터 2015년까지 국내 중부지방 시설재배지를 대상으로 지역별, 주요 작물별 뿌리혹선충의 감염 현황을 조사하였다. 총 132개 시설재배지 중에서 65개 포장(49%)에 뿌리혹선충이 감염되어 있었으며, 평균밀도는 토양 $100cm^3$ 당 뿌리혹선충 2기 유충 178마리로 나타났다(최저 1-최고 3,947마리). 시군별 감염률은 춘천이 80%로 가장 높았으며, 천안, 논산, 부여와 예산은 각각 68%, 36%, 33%와 30% 순으로 나타났다. 주요 작물별 뿌리혹선충 감염률은 토마토가 83%로 가장 높았으며, 오이(61%), 딸기(41%), 고추(30%), 수박(26%) 순으로 나타났다. 중부지방 시설재배지에서 검출된 뿌리혹선충 대표집단 15개체군의 분자생물학적 종 동정 결과, 고구마뿌리혹선충, 땅콩뿌리혹선충과 당근뿌리혹선충 3종이 각각 47%, 20%와 33%의 비율로 나타났다. 작물별로는 토마토에서 고구마뿌리혹선충, 땅콩뿌리혹선충과 당근뿌리혹선충 총 3종, 오이와 수박은 고구마뿌리혹선충과 땅콩뿌리혹선충 총 2종, 딸기와 상추에서는 당근뿌리혹선충 총 1종이 각각 검출되었다. 따라서, 뿌리혹선충 피해 확산 방지를 위해서는 작물별로 검출된 종을 고려한 방제대책 수립이 필요하다.

• The Plant Pathology Journal
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• 제36권3호
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• pp.297-302
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• 2020

Root-knot nematodes (Meloidogyne spp.) are the most destructive group of plant-parasitic nematodes. Plants infected by Meloidogyne spp. develop above-ground symptoms, stunting, yellowing, nutrient deficiencies, and gall formations with typical hook-shaped root tips. Infected plants experience yield losses. During 2018-2019 survey, leaf chlorosis rice plants were found in 206 fields of 67 counties in Guangxi, China, around 30 days after transplanting. Galls and hooked tips on the roots and pear-shaped females were observed. About 32.04% of fields were infested with the nematode. The nematodes were identified as Meloidogyne graminicola base on morphological and molecular analysis. To the best of our knowledge, this is the first report of M. graminicola on rice plants in Guangxi, China. The results of this study urge the discovery of resistant cultivars and the development of management strategies.

• 식물병연구
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• 제10권1호
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• pp.25-29
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• 2004

구미시 옥성면 국화 집단재배지에서 국화 생육불량 원인을 조사한 결과, 대부분의 국화(98％)가 뿌리혹선충(Meloidogyne hapla)에 감염되어 있었다. 주요 3품종 중 뿌리에 생긴 난낭의 수는 뿌리 1 g당 'Ardilo' 3.3개, 'Euro' 6.5개, 'Monarisa' 17개로 'Monarisa' 품종에서 난낭 발생이 많았고 'Ardilo' 품종에서 적었다. 국화의 초장, 화경장, 줄기 굵기, 엽수, 착화수, 첫 번째 화수장, 절화중, 엽록소(SPAD), 뿌리무게, 뿌리혹선충의 난낭수 사이의 상관관계를 조사한 결과 난낭수와 엽록소 함량(SPAD) 사이에 부의 상관이 인정되었다(r = -0.418, p = 0.059; r = -0.463, p=0.040). 식물 생육은 키가 크고 화경장이 길수록 꽃수, 잎수가 많고 꽃 무게도 무거웠다(p < 0.05).

• Journal of Plant Biotechnology
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• 제50권
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• pp.163-168
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• 2023

Sweetpotato (Ipomoea batatas [L.]) is a globally important root crop cultivated for food and industrial processes. The crop is susceptible to the root-knot nematode (RKN) Meloidogyne incognita, a major plant-parasitic RKN that reduces the yield and quality of sweetpotato. Previous transcriptomic and proteomic analyses identified several genes that displayed differential expression patterns in susceptible and resistant cultivars in response to M. incognita infection. Among these, several sporamin genes were identified for RKN resilience. Sporamin is a storage protein primarily found in sweetpotato and morning glory (Ipomoea nil). In this study, transcriptional analysis was employed to investigate the role of sporamin genes in the defense response of sweetpotato against RKN infection in three susceptible and three resistant cultivars. Twenty-three sporamin genes were identified in sweetpotato and classified as group A or group B sporamin genes based on comparisons with characterized sweetpotato and Japanese morning glory sporamins. Two group A sporamin genes showed significantly elevated levels of expression in resistant but not in susceptible cultivars. These results suggest that the elevated expression of specific sporamin genes may play a crucial role in protecting sweetpotato roots from RKN infection.

• 한국응용곤충학회지
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• 제20권4호
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• pp.223-227
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• 1981

These experiments were conducted to investigate the nematicidal effects of the insecticides, Mocap(O-Ethyl-S, S-dipropyl phosphorodithioate), Carbofuran(2,3-Dihydro-2,2-dimethyl benzofuranyl ethyl carbamate), Terbufos (S-tert-buthylthio methyl O,O-diethyl phosphordithioate) and their mixtures (Mocap+carbofuran, Mocap+Terbufos, Carbofuran+Terbufos) on growth of 2year-old ginseng, Panax ginseng C.A. Meyer, and the control of root-knot nematodes. There was no evidence of plant injury from insecticide treatment of ginseng, although the rate of emergence of the treated ginseng was slightly inhibited. The insecticide treatments showed no of-flavor of ginseng plant. Terbufos and Mocap provided heifer confrol of the root-knot nematodes than carbofuran alone and their mixtures. Mixtures of the insecticides showed antagonisitic effect to the root-knot nematodes.

• 한국유기농업학회지
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• 제27권4호
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• pp.501-512
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• 2019

To survey the incidence of plant-parasitic nematodes, 187 soil samples from leguminous plant fields in Chungnam province, Korea were assessed from June to August 2018. As the result, four nematode genera - the cyst nematode (Heterodera spp.), root-knot nematode (Meloidogyne spp.), root-lesion nematode (Pratylenchus spp.), spiral nematode (Helicotylenchus spp.) - were detected. Among them, the incidence of cyst nematode was the highest in Taean (73%) and lowest in Hongseong (10%). The cyst nematode was detected from the adzuki and kidney bean fields as well as soybean fields. The incidence of cyst nematode in upland fields was higher than in paddy fields. In addition, the extracted cyst nematodes consisted of two species, which was identified as Heterodera glycines and H. sojae. The incidence of H. glycines (22%) was higher than H. sojae (10%), and a few of the fields (5%) was mixed with two cyst nematodes. This result suggested that we should consider that the cyst nematode is the major plantparasitic nematode when controlling the nematodes in soybean fields in Chungnam province.

• The Plant Pathology Journal
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• 제37권6호
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• pp.596-606
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• 2021

Soil-borne diseases are the major problems in mono cropping. A mixture (designated LTM-m) composed of agricultural wastes and a beneficial microorganism Streptomyces saraceticus SS31 was used as soil amendments to evaluate its efficacy for managing Rhizoctonia solani and root knot nematode (Meloidogyne incognita). In vitro antagonistic assays revealed that SS31 spore suspensions and culture broths effectively suppressed the growth of R. solani, reduced nematode egg hatching, and increased juvenile mortality. Assays using two Petri dishes revealed that LTM-m produced volatile compounds to inhibit the growth of R. solani and cause mortality to the root knot nematode eggs and juveniles. Pot and greenhouse tests showed that application of 0.08% LTM-m could achieve a great reduction of both diseases and significantly increase plant fresh weight. Greenhouse trials revealed that application of LTM-m could change soil properties, including soil pH value, electric conductivity, and soil organic matter. Our results indicate that application of LTM-m bio-organic amendments could effectively manage soil-borne pathogens.

• The Plant Pathology Journal
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• 제30권4호
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• pp.450-455
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• 2014

This study sought to control the root-knot nematode (RKN) Meloidogyne incognita using benign organo-chemicals. Second-stage juveniles (J2) of RKN were exposed to dilutions (1.0%, 0.5%, 0.2%, and 0.1%) of acetic acid (AA), lactic acid (LA), and their mixtures (MX). The nematode bodies were disrupted severely and moderately by vacuolations in 0.5% of MX and single organic acids, respectively, suggesting toxicity of MX may be higher than AA and LA. The mortality of J2 was 100% at all concentrations of AA and MX and only at 1.0% and 0.5% of LA, which lowered slightly at 0.2% and greatly at 0.1% of LA. This suggests the nematicidal activity of MX may be mostly derived from AA together with supplementary LA toxicity. MX was applied to chili pepper plants inoculated with about 1,000 J2, for which root-knot gall formations and plant growths were examined 4 weeks after inoculation. The root gall formation was completely inhibited by 0.5% MX and standard and double concentrations of fosthiazate; and inhibited 92.9% and 57.1% by 0.2% and 0.1% MX, respectively. Shoot height, shoot weight, and root weight were not significantly ($P{\leq}0.05$) different among all treatments and the untreated and non-inoculated controls. All of these results suggest that the mixture of the organic acids may have a potential to be developed as an eco-friendly nematode control agent that needs to be supported by the more nematode control experiments in fields.

• 한국응용곤충학회지
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• 제43권1호
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• pp.27-34
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• 2004

더덕(Codonopsis lanceolata) 재배지에서 당근뿌리혹선충의 환경친화적 방제법을 구명코자 방제실험을 수행한 결과 당근뿌리혹선충 방제 실험에 사용된 방제제들(Bacillus thuringiensis, Paecilomyces lilacinus, fosthiazate, 한약제 추출물) 중 더덕의 발아를 향상시키는 것을 Bt와 한약제 추출물이었고, 억제시키는 것은 fosthiazated였다. Pot에서 미생물제에 의한 당근뿌리혹선충 밀도억제 효과는 Bt와 Paecilomyces lilacinus 모두 우수하였으나 fosthiazate 보다는 떨어지는 경향이었다. 또한 한약제 추출물도 방제효과가 우수하였다. 포장실험에서도 Paecilomyces lilacinus와 fosthiazate 처리 시 수확량이 가장 많았으며 수확된 더덕의 품질도 가장 우수하였다. 한약제추출물도 무처리에 비하여 더덕의 수량 증대에 효과가 있었고, 뿌리혹선충 감염지에서 키운 더덕을 이식한 것이 비감염지에서 키워 이식한 더덕에 비하여 수량 증가율이 저조하였다.