• 식물조직배양학회지
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• 제27권3호
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• pp.233-238
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• 2000

야생 흰제비꽃의 엽병 유래 callus를 장기 계대배양하는 과정 중에 발생하는 순화된 friable callus와 분화능이 높은 compact callus를 비교 관찰한 바, friable callus는 연초록색으로 부서지기 쉬운 부드러운 callus이고, compact callus는 진녹색으로 단단한 callus였다. 동결처리 한 시료를 주사전자 현미경에서 동일하게 200배로 관찰하여 보면, friable callus 는 작은 세포집단으로 이루어진 세포군의 주변부에 고도로 액포화된 세포가 광범위하게 분포되어 있는 반면, compact callus는 거의 균일한 세포들로 구성되어 세포구성이 치밀한 것으로 관찰되었다. 또한 friable callus와 compact callus로 부터의 체세포배형성은 배양세포에서 배가 발생하여 배양기간이 지남에 따라 식물체로 분화하였다. 이와 같은 과정은 배양세포의 세포질이 보다 충만한 부위에서 배유사체 (embryo like body)의 발생이 이루어지는 것으로 관찰되었다.

• Journal of Plant Biology
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• 제14권3호
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• pp.29-35
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• 1971

Various types of short culm rice mutants were obtained by means of gamma-ray irradiation. In the present paper morphological observation and analysis of silicate content of the mutants are reported. 1. Short culm type had more useful characters than bushy and dwarf types. 2. In short culm and bushy types the number of nodes both above and under ground was similar to the mother varieties, while in dwarf type it decreased. 3. In short culm types the variation of length of above-ground culm and internodes tended to vary relattive to the mother varieties. 4. Positive correlation was found between culm length and the first and fourth internode length in short culm type. 5. Silicate content increased in short culm type-the increment being variety specific.

• 한국자원식물학회지
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• 제3권2호
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• pp.123-128
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• 1990

In every reciprocal crosses of self pollination, interspecific pollination through ordinary stigma pollination of L. longiflorum and $L.{\;}{\times}{\;}elegans$, pollen vigorously germinated in stigma, and pollen tube was growing. But, 5 days after pollination, pollen tube stopping their growth in the same style as was observed in the cross of self pollination and interspecific pollination. Intrastylar pollination of $L.{\;}{\times}{\;}elegans$ and L. longiflorum passed stylar cannal through the basal part of styles. But, pollen tube that was growing to the ovule of the ovary was extremely small. In morphogical observation of ovary 10 days after pollination, it was observed, that every reciprocal crosses of L. longiflorum ${\times}$ L. ${\times}$ elegans was fertilized through intrastylar pollination, so that this technique was used to overcome cross incompatibilities.lities.

• Journal of Plant Biology
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• 제13권3호
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• pp.17-19
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• 1970

Histological observation of micropore-originated haploid rice callus was reported previously. Present study was attempted to clarify the growth or development of the calli when they were transferred to differentiation media prepared exclusively for differentiation of plantlets. When the callus was transferred to differentiation medium, the cells and tissues became radially elongated. Meristematic tissues were present but few in number, and their structures were quite different from those grown in the propagaton medium. Differentiation of tracheid, chloroplast, and epidermis-like cell layer, and formation of gap in the callus tissue were more conspicuous in differentiation media. Approximately ten days after transfer of callus to differentiation medium, plantlet was formed.

• Asian-Australasian Journal of Animal Sciences
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• 제10권6호
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• pp.593-598
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• 1997

The changes of tissue structure in timothy and alfalfa during ensiling process with silage additives; lactic acid bacteria, cellulase and formic acid, were observed with a video microscope. Stem samples were obtained from the second internode, and cut to divide into 2 pieces. One piece was for observation of ensiled material and the other was for silage. The latter piece was put into a nylon cloth bag, and ensiled with grass for 50 days in a small experimental silo Lignification of the plant tissues was checked by acid phloroglucinol. Natural silage fermentation resulted in some degradation of less lignified parenchyma in both plant species. However, lignified sclerenchyma and vascular bundles remained intact. The cellulase enhanced the degradation of parenchyma tissue, while the formic acid suppressed the degradation. The effect of lactobacillus was small. The percentage of remained cross sectional area of stem and the loss of NDF and ADF by silage fermentation confirmed the observation. High negative correlations were obtained between the remained area and loss of fibrous components during silage fermentation in both plants, and between the loss of fibrous components and in vitro dry matter digestibility in timothy but not in alfalfa.

• Journal of Microbiology and Biotechnology
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• 제6권6호
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• pp.468-473
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• 1996

The genome of tomato spotted wilt virus (TSWV) is composed of three RNA segments, S, M, and L RNA and the 5.0 kb M RNA encodes two glycoproteins Gl, G2 and NSm protein of unknown function. In an effort to investigate the function of the NSm protein, antibody was raised against NSm fusion protein overexpressed in Escherichia coli. This antibody was used to detect the NSm protein by using western blot analysis and electron microscopic observation after immunogold labelling. For the cloning of the NSm gene, total RNA extracted from a TSWV infected plant was used for cDNA synthesis and polymerase chain reaction (PCR) instead of going through time-consuming virus purification. A protein band specifically reacting to the NSm antibody was detected from TSWV inoculated plants. The NSm protein was detected in the cell wall fraction and in pellet from low speed centrifugation when the infected plant tissue was fractionated into 4 fractions. In the immuno-electron microscopic observation, gold particles were found around the plasmodesmata of infected plant tissue. These results suggest that the NSm protein of TSWV plays some role in cell-to-cell movement of this virus.

• 농업생명과학연구
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• 제51권6호
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• pp.15-22
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• 2017

The microstructure observation of seed surface structure is needed for protocols of breaking dormancy of seeds with physical dormancy. The seeds of Rubus species are surrounded by a thick, hard endocarp; together, the seed and endocarp make up the stone. We evaluate stone characteristics of 18 species of Rubus through optical microscopic observation, and correlate different stone characteristics with endocarp thickness. As a result of stone size comparison, Rubus species were classified as big stones group including R. parvifolius and R. idaeus, small stones group including R. longisepalus var. longisepalus, R. corchorifolius and R. hirsutus, and middle stones group including rest of the species. The result of this study revealed that stone size and the endocarp thickness in Rubus species was various characteristics in each species. Furthermore stone size and stone weight were also well correlated endocarp thickness and result indicated that heavy stones had harder endocarp than lighter one. Thus from the result of this study it can be presumed that only one stone characteristic approach may be sufficient to estimate other characteristics in Rubus.

• Ecology and Resilient Infrastructure
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• 제10권4호
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• pp.226-236
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• 2023

기후변화가 심화됨에 따라 식물계절연구의 중요도가 증가하고 있으며 자동영상촬영장치 (피노캠, Phenocam)을 활용한 연구방법이 급부상하고 있다. 본 연구에서는 국립생태원에서 운영하는 피노캠을 활용하여 국내 주요 생태계 유형에 대한 식물계절 변화의 경향을 확인하고 기후요인과의 상관관계를 분석했다. 식물계절의 변화 양상은 지역 및 수종별로 다르게 나타났다. 곰솔 및 소나무림은 전체 생장 기간이 증가하는 경향을 보이며 주로 겨울철 기온과 강수량과 양의 상관관계를 보였으나, 한라산 구상나무는 8월 강수량이 많을수록 생장종료일이 빨라졌으며 최근 발생하는 구상나무 고사 현상과의 연관성 분석이 추후 필요할 것으로 보인다. 분석 결과에서 더 나아가 데이터 수집 과정에서 발생할 수 있는 결측치 문제 등에 대한 해결책을 제시하였으며, 향후 연구 범위를 확장하고 다양한 생태계 유형을 반영하기 위해 피노캠 연구와 위성 관측을 결합하는 방안을 제안하였다.

• 식물병연구
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• 제20권2호
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• pp.129-131
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• 2014

In February 2014, gray mold rotting symptoms were observed in carrots in cold storage at Gangneung, Gangwon province, Korea. The typical symptom of gray mold rot showed abundant blackish gray mycelia and conidia was observed on the infected root. The pathogen was isolated from infected root and cultured on PDA for further fungal morphological observation and confirming its pathogenicity according to Koch's postulates. Results of morphological data, pathogenicity test and rDNA internal transcribed spacer (ITS 1 and 4) sequence showed that the postharvest gray mold rot of carrot was caused by Botyrtis cinerea. This is the first report of postharvest gray mold rot on carrot in Korea.

• 한국환경과학회지
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• 제27권3호
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• pp.227-231
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• 2018

We studied the warming effect induced by Photovoltaic(PV) power plants in rural areas during summer daytime using a simple analytical urban meteorological model. This analysis was based on observed meteorological elements and the capacity of the PV power plant was 10 MWp. The major axis length of the PV power plant was assumed to be 1km. Data of the necessary meteorological elements were obtained from a special meteorological observation campaign established for a over a PV power plant. We assumed that the wind flowed along the major axis of the PV power plant(1 km). As a result, the air temperature on the downwind side of the PV power plant was estimated to invrease by about $0.47^{\circ}C$.