• The Plant Pathology Journal
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• 제38권4호
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• pp.372-382
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• 2022

Soybean is an important source of protein and for a wide range of agricultural, food, and industrial applications. Soybean is being affected by Xanthomonas citri pv. glycines, a causal pathogen of bacterial pustule disease, result in a reduction in yield and quality. Diverse microbial communities of plants are involved in various plant stresses is known. Therefore, we designed to investigate the microbial community differentiation depending on the infection of X. citri pv. glycines. The microbial community's abundance, diversity, and similarity showed a difference between infected and non-infected soybean. Microbiota community analysis, excluding X. citri pv. glycines, revealed that Pseudomonas spp. would increase the population of the infected soybean. Results of DESeq analyses suggested that energy metabolism, secondary metabolite, and TCA cycle metabolism were actively diverse in the non-infected soybeans. Additionally, Streptomyces bacillaris S8, an endophyte microbiota member, was nominated as a key microbe in the healthy soybeans. Genome analysis of S. bacillaris S8 presented that salinomycin may be the critical antibacterial metabolite. Our findings on the composition of soybean microbiota communities and the key strain information will contribute to developing biological control strategies against X. citri pv. glycines.

• BMB Reports
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• 제57권1호
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• pp.30-39
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• 2024

Directed evolution (DE) of desired locus by targeted random mutagenesis (TRM) tools is a powerful approach for generating genetic variations with novel or improved functions, particularly in complex genomes. TRM-based DE involves developing a mutant library of targeted DNA sequences and screening the variants for the desired properties. However, DE methods have for a long time been confined to bacteria and yeasts. Lately, CRISPR/Cas and DNA deaminase-based tools that circumvent enduring barriers such as longer life cycle, small library sizes, and low mutation rates have been developed to facilitate DE in native genetic environments of multicellular organisms. Notably, deaminase-based base editing-TRM (BE-TRM) tools have greatly expanded the scope and efficiency of DE schemes by enabling base substitutions and randomization of targeted DNA sequences. BE-TRM tools provide a robust platform for the continuous molecular evolution of desired proteins, metabolic pathway engineering, creation of a mutant library of desired locus to evolve novel functions, and other applications, such as predicting mutants conferring antibiotic resistance. This review provides timely updates on the recent advances in BE-TRM tools for DE, their applications in biology, and future directions for further improvements.

• 한국원자력학회:학술대회논문집
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• 한국원자력학회 1998년도 춘계학술발표회논문집(2)
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• pp.795-801
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• 1998

Metallic fatigue of Pressurized Water Reactor(PWR) materials is a generic safety issue for commercial nuclear power plants. It is very important to obtain the fatigue usage factor for component integrity and life extension. In this paper, fatigue usage was obtained at the inside surface of Kori unit 2, 3 and 4 RCP casing weld, based on the design transient. And it was intended to establish the procedure and the detailed method of fatigue evaluation in accordance with ASME Section III Code. According to this code rule, two methods to determine the stress cycle and the number of cycles could be applied. One method is the superposition of cycles of various design transients and the other is based on the assumption that a stress cycle correspond to only one design transient. Both method showed almost same fatigue usage in the RCP casing weld.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.123-123
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• 2018

Premenstrual syndrome (PMS) is a common disorder affecting the emotional and physical health of women during certain periods of the menstrual cycle. Many researchers who have previously studied PMS have believed that PMS is associated with changes in sex hormones and serotonin levels at the beginning of the menstrual cycle. However, recent studies suggest that progesterone/estrogen imbalance and elevation of prolactin-induced by dopamine low-secretion play a crucial role in increasing PMS symptoms. Because of this, we have focused on mitigating PMS symptoms through the mechanism of prolactin secretion inhibition by dopamine receptor activation. The inhibition of prolactin secretion by 61-kinds of medicinal herb extracts was investigated in GH3 pituitary cells. Among them, Inula heleniun L. root extract (IHE) showed excellent prolactin secretion inhibitory effect. IHEs were prepared using 30, 50, and 70% ethanol. And the yield, cytotoxicity, dopamine receptor activity and inhibition of prolactin secretion of each extract were measured. Through a series of experiments, we found that prolactin secretion was significantly reduced (P<0.01) by the components present in IHE and that dopamine receptor regulation was possible (P<0.05). Considering yield and safety, we suggest the use of 30% ethanol IHE in the development of PMS symptom relief products.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.80.2-80.2
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• 2008

• The Plant Pathology Journal
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• 제40권2호
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• pp.99-105
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• 2024

Land plants produce glucose (C₆H₁₂O₂) through photosynthesis by utilizing carbon dioxide (CO₂), water (H₂O), and light energy. Glucose can be stored in various polysaccharide forms for later use (e.g., sucrose in fruit, amylose in plastids), used to create cellulose, the primary structural component of cell walls, and immediately metabolized to generate cellular energy, adenosine triphosphate, through a series of respiratory pathways including glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation. Additionally, plants must metabolize glucose into amino acids, nucleotides, and various plant hormones, which are crucial for regulating many aspects of plant physiology. This review will summarize the biosynthesis of different plant hormones, such as auxin, salicylic acid, gibberellins, cytokinins, ethylene, and abscisic acid, in relation to glucose metabolism.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1999년도 춘계 학술대회지
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• pp.162-163
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• 1999

The goal of this research was (1) to describe the conditions and parameters required for the cell cycle synchronization and the accumulation of large number of metaphase cells in maize and other cereal root tips, (2) to isolate intact metaphase chromosomes from root tips suitable for characterization by flow cytometry, and (3) to construct chromosome-specific libraries from maize. Plant metaphase chromosomes have been successfully synchronized and isolated from many cereal root-tips. DNA synthesis inhibitor (hydroxyurea) was used to synchronize cell cycle, follwed by treatement with trifluralin to accumulate metaphase chromosomes. Maize flow karyotypes show substantial variation among inbred lines. thish variation should be sueful in isolating individual chromosome types. In addition, flow cytometry is a useful method to measure DNA content of individual chromosomes in a genotyps, and to detect chromosomal variations. Individual chromosome peaks have been sorted from the maize hybrid B73/Mol7. Libraries were generated form the DOP-PCR amplification product from each peak. To date, we have analyzed clones from a library constructed from the maize chromosome 1 peak. Hybridization of labeled genomic DNA to clone inserts indicated that $24\%,\;18\%,\;and\;58\%$ of the clones were highly repetitive, medium repetitive, and low copy, respectively. Fifty percent of putative low cpoy clones showed single bands on inbred screening, blots, and the remaining $50\%$ were low copy repeats. Single copy clones showing polymorphism will be mapped using recombinant inbred mapping populations. Repetitive clones are being characterized by Southern blot analysis, and will be screened by in situ hybridization for their potential utility as chromosome specific markers.

• 플랜트 저널
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• 제15권2호
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• pp.46-55
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• 2019

기존 메탄 & 질소 팽창 사이클의 효율 개선을 위해 2개의 메탄 팽창 공정 (cold composite curve에 변곡점을 하나 더 추가하기 위해 warm & cold 2개 공정으로 나눔)과 1개의 질소 팽창 공정을 사용한 천연가스 액화 사이클을 소개하기 위한 논문이다. 이전 질소 팽창 사이클 및 메탄 & 질소 사이클과 비교했을 때, 13.92 및 13.13에서 12.08 kW/ton/day로 효율이 8~15% 정도 개선되었다. 순 현재가치(NPV) 기준으로 한 수명 주기 비용 분석(LCC analysis) 또한 약간의 CAPEX 증가는 있지만 프로젝트 순 현재가치가 개선된 결과를 보여준다.

• 한국건축시공학회지
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• 제14권6호
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• pp.583-590
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• 2014

본 연구에서는 경제적인 창호공사 방안을 확인하기 위해 단열필름이 시공된 창호유리와 일반유리의 생애주기비용을 분석하여 비교하고자 하였다. 이를 위해 Window 6.3과 ECO2-OD 시뮬레이션 프로그램을 사용하여 유리 유형별 창호의 열성능 데이터를 측정하고 사례 건물에 적용하여 냉 난방 유지비용 및 LCC를 산출하여 경제성을 비교하였다. 연구결과 단열필름을 추가적으로 시공함으로써, 냉 난방 유지비용 측면에서는 하절기 태양열이 실내로 투과하는 것을 막아 냉방비용 절감 효과가 있지만, 이로 인해 동절기에는 난방비용이 증가하는 것으로 나타났다. 생애주기비용 측면에서 볼 때, 냉방비용 절감 효과가 난방비용 증가량과 필름 시공 및 수선으로 발생하는 추가비용을 상쇄하지 못하기 때문에 단열필름의 시공은 적절한 방법이 아니라고 할 수 있다.

• 상하수도학회지
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• 제27권2호
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• pp.223-232
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• 2013

In this study, carbon emissions and energy consumption were evaluated to establish a design strategy which has low energy consumption and carbon emission production, by using life cycle energy (LCE) and life cycle $CO_2(LCCO_2)$ calculation methods in life cycle management(LCM) tools. After improvement design projects, the energy consumption and $CO_2$ emission were calculated and compared in three sewage treatment plants (STPs), which are A STP, B STP, and C STP. The reduction of carbon emissions was 28,020.1 ton $CO_{2-}e/yr$, 37,399.6 ton $CO_{2-}e/yr$ and 8,788.3 ton $CO_{2-}e/yr$, respectively. Production of energy was 792 TOE/yr, 1,235 TOE/yr and 1,023 TOE/yr, respectively. As a result, the estimation of energy and energy self-sufficiency was 5.1 %, 14.5 % and 23.5 %, respectively. The result of this study shows the LCM can be contributed to establish strategy for energy and carbon emission reduction in sewage treatment plants.