• Journal of Mushroom
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• v.17 no.2
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• pp.47-51
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• 2019

Our research on juvenile Oncorhynchus masou masou in oyster mushroom supplemented diet was studied to investigate the effect of feeding. Mixing of feed ingredients for dried oyster mushrooms, 3.5, 7.0, 10.5, 14.0 (%) was added to the amount of oyster mushroom dietary beta-glucan content of the more abundant. After the weight of feed given to salmon survey oyster mushrooms diet for 3.5 to 7.0% was similar to the formula feed and the weight of the fish ate oyster mushroom feed over 10.5% were reduced. The oyster mushroom of the experimental diets containing 3.5 to 7.0 percent hepatosomatic index and feed coefficient figure was similar to those of the formulated diets. Therefore we have juvenile cherry salmon fed diets containing 3.5 to 7.0% was considered good to eat and additional research on the immune response will be carried out was necessary.

• Korean Journal of Veterinary Research
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• v.63 no.1
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• pp.5.1-5.9
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• 2023

Feline panleukopenia virus (FPV), feline calicivirus (FCV), and feline herpesvirus type-1 (FHV-1) are major infectious pathogens in cats. We evaluated the immunogenicity of a new vaccine containing inactivated FPV, two FCVs, and FHV-1 in animals. An FPV, two FCVs, and an FHV-1 isolate were continuously passaged 70, 50, 80, and 100 times in CRFK cells. FP70, FC50, FC80, and FH100 were propagated and used as vaccine antigens. Two inactivated feline virus vaccines, feline rehydragel-adjuvanted vaccine (FRAV) and feline cabopol-adjuvanted vaccine (FCAV) were prepared and inoculated into mice and guinea pigs. Humoral immune responses were measured using hemagglutination inhibition (HI) for FPV and virus-neutralizing antibody (VNA) for two FCVs and FHV-1 tests. Serial passages in CRFK cells resulted in increase in titers of FPV and two FCVs but not FHV-1 The FCAV induced higher mean HI and VNA titers than the FRAV in guinea pigs; therefore, the FCAV was selected. Cats inoculated with FCAV developed a mean HI titer of 259.9 against FPV, and VNA titers of 64, 256, and 3.2 against FCV17D03, FCV17D283, and FHV191071, respectively. Therefore, cats inoculated with the FCAV showed a considerable immune response after receiving a booster vaccination.

• Journal of Microbiology and Biotechnology
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• v.26 no.12
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• pp.2214-2223
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• 2016

Toll-like receptors (TLRs) can recognize conserved molecular patterns and initiate a wide range of innate and adaptive immune responses against invading infectious agents. The aim of this study was to assess the transcript profile of mink TLRs (mTLRs) in mink peripheral blood mononuclear cells (PBMCs) and a range of tissues, and to explore the potential role of mTLRs in the antiviral immune response process. The results indicated that the mTLR partial nucleotide sequences had a high degree of nucleotide identity with ferret sequences (95-98%). Phylogenetic analysis showed that mammalian TLRs grouped into five TLR families, with a closer relationship of the mTLRs with those of ferret than the other mammalian sequences. Moreover, all the mTLRs were ubiquitously expressed in lymphoid organs (spleen and lymph nodes) and PBMCs. Interestingly, the mTLR expression patterns in lung, uterus, and heart showed quite a lot of similarity. Another remarkable observation was the wide expression of mTLR1-3 mRNAs in all tissues. Among the analyzed tissues, skeletal muscle was revealed to being the lowest repertoire of mTLR expression. Additionally, mink PBMCs exposed to the canine distemper virus revealed significant upregulation of mTLR2, mTLR4, mTLR7, and mTLR8 mRNAs, indicating that mTLRs have a role in innate immunity in the mink. Collectively, our results are the first to establish the basic expression patterns of mTLRs and the relationship between mTLRs and a virus, which will contribute to better understanding of the evolution and the functions of mTLRs in the innate immune system in minks.

• 제어로봇시스템학회:학술대회논문집
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• 2002.10a
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• pp.70.2-70
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• 2002

In the thermal power plant, it is difficult to maintain strict control of the steam temperature in order to avoid thermal stress, because of variation of the heating value according to the fuel source, the time delay of changes in main steam temperature versus changes in fuel flow rate, difficulty of control on the main steam temperature control and the reheater steam temperature control system owing to the dynamic response characteristics of changes in steam temperature and the reheater steam temperature, fluctuation of inner fluid water and steam flow rates widely during load-following operation. Up to the present time, the PID controller has been used to operate this system...

• Molecules and Cells
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• v.46 no.11
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• pp.710-724
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• 2023

The plant defense responses to microbial infection are tightly regulated and integrated with the developmental program for optimal resources allocation. Notably, the defense-associated hormone salicylic acid (SA) acts as a promoter of flowering while several plant pathogens actively target the flowering signaling pathway to promote their virulence or dissemination. Ralstonia pseudosolanacearum inject tens of effectors in the host cells that collectively promote bacterial proliferation in plant tissues. Here, we characterized the function of the broadly conserved R. pseudosolanacearum effector RipL, through heterologous expression in Arabidopsis thaliana. RipL-expressing transgenic lines presented a delayed flowering, which correlated with a low expression of flowering regulator genes. Delayed flowering was also observed in Nicotiana benthamiana plants transiently expressing RipL. In parallel, RipL promoted plant susceptibility to virulent strains of Pseudomonas syringae in the effector-expressing lines or when delivered by the type III secretion system. Unexpectedly, SA accumulation and SA-dependent immune signaling were not significantly affected by RipL expression. Rather, the RNA-seq analysis of infected RipL-expressing lines revealed that the overall amplitude of the transcriptional response was dampened, suggesting that RipL could promote plant susceptibility in an SA-independent manner. Further elucidation of the molecular mechanisms underpinning RipL effect on flowering and immunity may reveal novel effector functions in host cells.

• Animal Bioscience
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• v.36 no.4
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• pp.540-554
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• 2023

Heat stress is a major challenge to animal production in tropical and subtropical climates. Rabbits suffer from heat stress more than farm animals because they have few sweat glands, and their bodies are covered with thick fur. Intensive farming relies on antibiotics as antimicrobials or growth promoters to increase animals' productivity and health. However, the European Union and many countries have banned or restricted the use of antibiotics in animal feed for human health concerns. Several studies have found that replacing antibiotics in rabbit feed with natural plants or feed additives increases productivity and improves immune capacity, especially under heat stress conditions. Growth performance, immune response, gut microflora, and carcass yield may be increased in rabbits fed a diet supplemented with some natural plants and/or propolis. In this review article, we discuss and summarize the effects of some herbs and plant extracts as alternative feed additives on rabbit productivity, especially for those raised under hot ambient temperatures.

• International Journal of Industrial Entomology and Biomaterials
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• v.25 no.2
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• pp.139-146
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• 2012

Botanicals have been regarded as sustainable solution of pest control because of their nominal effect on nontarget pest, biodegradability and other environment friendly properties. However, study on impact of botanicals against beneficial insects is less. In this investigation effort has been made to study the effect of essential oils of Ocimum sanctum, Ocimum gratissimum and Ageratum conyzoides against non-mulberry silkworm Antheraea assama in terms of percent mortality, total haemocyte count (THC) and phenol oxidase (PO) enzyme activity. After application of essential oil of the plants by contact residual film technique, age dependent decrease of percent mortality was recorded. Topical treatment of oil at higher dosages ($1{\mu}lg^{-1}$ and $0.5{\mu}lg^{-1}$) caused 100% mortality of late instar larvae at 24 hour but the lowest dose ($0.25{\mu}lg^{-1}$) did not cause any mortality till 48 hour of treatment. Initial increase of total haemocyte count (THC) was recorded at early hours of treatment (0.25 hour) at all dosages (1, 0.5, $0.25{\mu}lg^{-1}$) after topical treatment. A dose dependent increase was observed in phenol oxidase enzyme activity and this might indicate ability of the essential oils to cause induction of immune response in Antheraea assama larvae.

• Molecules and Cells
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• v.43 no.4
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• pp.313-322
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• 2020

Eukaryotes transport biomolecules between intracellular organelles and between cells and the environment via vesicle trafficking. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE proteins) play pivotal roles in vesicle and membrane trafficking. These proteins are categorized as Qa, Qb, Qc, and R SNAREs and form a complex that induces vesicle fusion for targeting of vesicle cargos. As the core components of the SNARE complex, the SNAP25 Qbc SNAREs perform various functions related to cellular homeostasis. The Arabidopsis thaliana SNAP25 homolog AtSNAP33 interacts with Qa and R SNAREs and plays a key role in cytokinesis and in triggering innate immune responses. However, other Arabidopsis SNAP25 homologs, such as AtSNAP29 and AtSNAP30, are not well studied; this includes their localization, interactions, structures, and functions. Here, we discuss three biological functions of plant SNAP25 orthologs in the context of AtSNAP33 and highlight recent findings on SNAP25 orthologs in various plants. We propose future directions for determining the roles of the less well-characterized AtSNAP29 and AtSNAP30 proteins.

• The Plant Pathology Journal
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• v.23 no.2
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• pp.62-69
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• 2007

Twenty-nine isolates of Fusarium solani, originally isolated from diseased cotton roots in Egypt, were evaluated for their ability to cause symptoms on four genetically diverse cotton cultivars. Analysis of variance showed highly significant variance among cultivars, and isolates as well as the isolate x genotype interactions were highly significant(p < 0.0001). Although most isolates showed intermediate pathogenicity, there were two groups of isolates that showed significant differences in pathogenicity on all four cultivars. None of the cultivars were found to be immune to any of the isolates. On all cultivars, there were strong significant positive correlations between dry weight and each of preemergence damping-off, survival, and plant height. Considering 75% similarity in virulence, two groups comprising a total of 29 isolates were recognized. Ninety-three percent of the isolates have the same pathogenicity patterns with consistently low pathogenicity, and narrow diversity of virulence. Isolates Fs4 and Fs5 shared the same distinct overall virulence spectrum with consistently high pathogenicity. There was no clear-cut relationship between virulence of the isolates based on reaction pattern on 4 cultivars and each of host genotype, previous crop, and geographic origin.

• Korean Journal of Plant Resources
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• v.25 no.5
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• pp.578-585
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• 2012

Cytokines released from innate immune cells play key roles in the regulation of the immune response. Red Ginseng (RG, steamed and dried root of Panax ginseng C.A. Meyer) is known to show different pharmacological effects by changed composition of saponins compared with Panax Ginseng. In this study, we examined the immunomodulatory effects of RG on the regulation of cytokine release in mice. RG was injected i.p at doses of 0.5, 5 and 50 mg/kg for 6 weeks. We assessed that the weight index of immune organs such as thymus, and spleen, and the mitogen blastogenesis of splenocytes. We also determined the levels of circulating cytokines in serum from RG-treated mice using ELISA assay. The weight index of thymus and spleen, and proliferation of mitogen response of splenocytes have increased in the RG-injected groups. In addition, the levels of IFN-${\gamma}$, TNF-${\alpha}$, IL-6, IL-12 and IL-2 concentrations have significantly increased in the serum of RG-treated mice, but that of IL-10 has not. These results suggest that RG has immune stimulating effects and could be useful as a immunoregulator of circulating cytokine release in vivo.