• Journal of Plant Biotechnology
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• 제6권2호
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• pp.87-90
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• 2004

An efficient plant regeneration system in alfalfa (Medicago sativa L.) through somatic embryogenesis was established. Embryogenic callus was obtained by culture of hypocotyl segments on MS medium with 0.02mg $L^{-1}$ IAA and 1.0mg $L^{-1}$ zeatin after 45 days of culture. Embryogenic calli were converted to the somatic embryos when transferred to either MS medium without plant growth regulators (PGRs) or MS medium containing various cytokinin (BA, kinetin and zeatin). Most of the somatic embryos were developed into plantlets on MS medium supplemented with 0.1 mg $L^{-1}$ kinetin. Also, secondary embryos appeared on the surface of primary embryo but they showed abnormal growth. Regenerated plantlets were transplanted to pots containing vermiculite and perlite for further analysis.

• Current Research on Agriculture and Life Sciences
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• 제32권4호
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• pp.179-183
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• 2014

Optimization of the protocorm-like body (PLB) formation of Phalaenopsis and Dendrobium hybrids was performed by determining the effects of plant growth regulators (PGRs) and different parts and division sizes of the PLB. For both genera, the base part was the best for the proliferation of PLBs, yielding the highest number of PLBs on a PGR-free medium for Phalaenopsis and medium containing $0.1{\mu}M$ NAA and $10.0{\mu}M$ BAP for Dendrobium. As regards the division size, four-division sections resulted in a higher PLB formation efficiency for Phalaenopsis, while two-division sections produced a higher PLB formation efficiency for Dendrobium. It is expected that these findings will be applicable to efficient PLB formation of other Phalaenopsis and Dendrobium orchids.

• Plant Resources
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• 제8권2호
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• pp.81-86
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• 2005

An efficient plant regeneration system of Gentiana scabra through somatic embryogenesis was established. Leaves and roots of seedlings of Gentiana scabra excised after germination were cultured on MS basal medium with 2,4-D, NAA or BA. Embryogenic callus was obtained on MS medium with 0.5 mg/L 2,4-D alone or 0.1 mg/L 2,4-D combimation with 1.0 mg/L BA after 45 days of culture. These embryogenic calli gave rise to somatic embryos, which subsequently developed into plantlets on MS medium without PGRs. Also, shoots were effectively differentiated from embryogenic callus when root segments were cultured on MS medium supplement with 0.1 mg/L 2,4-D and 1.0 mg/L BA. Shoots were effectively rooted on MS medium without PGRs. In vitro flowers were formed from plantlets cultured on MS medium with $5\%$ sucrose after 60 days of culture.

• 한국작물학회지
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• 제62권3호
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• pp.259-274
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• 2017

Platycodon grandiflorum (Bell flower) is an important plant that has traditionally been used as herbal medicine for the treatment of cough, phlegm, sore throats, lung abscesses, chest pains, dysuria, and dysentery. The present study was initiated to investigate the feasibility of inducing shoot and root organogenesis in cultured explants of P. grandiflorum in a range of culture media and through use of various plant growth regulators (PGRs). The plantlets (Stem containing one node) were isolated and cultured on different concentrations of Murashige and Skoog (MS) medium supplemented with PGRs. We found that proliferation and elongation of shoots and roots could be achieved on 1/4 MS for P. grandiflorum with wild and green petals and on 1/8 MS for P. grandiflorum with double petals. The highest levels of development and elongation of adventitious shoots and roots were observed when petal explants were cultured on 1/4 MS (pH 3.8) supplemented with 5% sucrose. Increasing the agar concentration reduced shoot growth and rooting potential; nevertheless, the highest number of shoots and roots was observed on 0.6% agar. In the case of growth regulators, 1/4 MS supplemented with $1mg\;L^{-1}$ 6-benzylaminopurine (BA) was found to be best for shooting, although higher concentrations of BA tended to reduce shoot and root elongation. The highest number of shoots was achieved on $0.5mg{\cdot}L^{-1}$ thidiazuron (TDZ) from double petal explants grown on 1/8 MS. However, root and shoot elongation were found to decrease when TDZ concentrations were increased. Low concentrations of kinetin, naphthalene acetic acid, indole acetic acid, and 3-indole butyric acid induced shoot and root proliferation and elongation. Taken together, our study showed that low concentrations of PGRs induced the greatest root formation and elongation, showing that the optimal concentration of PGRs for shoot proliferation was species-dependent.

• 한국작물학회지
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• 제44권3호
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• pp.225-229
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• 1999

Unstable seedling stand establishment of wet direct seeding culture of rice is one of the major elements preventing the extension of its culture area. In order to develop methods of seedling stand improvement in direct seeded rice on flooded surfaces, three field experiments were conducted on silty loam soil using a cultivar 'Donjinbyeo' for three years, mainly focusing on water management after seeding and seed soaking with plant growth regulators (PGRs). Under the condition of shallow flooding after seeding, seedling stand rate increased and floating seedling rate decreased in both early and normal season seeding compared to deep flooding. With earlier draining time after seeding, there was a tendency towards preferential growth of the seminal root, increase of seedling stand and decrease of the floating seedling rate. Therefore the highest seedling numbers per unit area and the lowest floating seedling numbers were found upon drainage at 1 day after seeding (DAS), while a contrary tendency was shown upon conventional drainage at 7 DAS. Seed soaking with PGRs such as Metalaxyl or mixing of Metalaxyl with gibberellic acid (GA$_3$) significantly increased the seedling stand. In addition the effects of PGR treatment on seedling stand and the early growth of plants were greater under flooded conditions than under drained conditions after seeding, although draining of water after seeding improved the seedling establishment rate more when compared with the PGR treatment. These results suggest that draining management after seeding or maintaining of shallow flooding for a week is the most effective method to improve the seedling stand rate in wet direct seeding.

• 한국환경복원기술학회지
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• 제19권1호
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• pp.101-109
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• 2016

This is the fundamental research on restoring damaged vegetation areas in the vicinity of DMZ using local native plant species. This research is aimed at identifying effective plant growth regulators (PGR) for seed germination and seedling growth of mountain mulberry, and developing effective methods for managing its germination and growth. Mountain mulberry seeds were collected from the regions in the DMZ vicinity, and tests with seven treatments using four PGRs including $GA_3$, kinetin, ABA and NAA, were conducted. The germination rate was calculated in two different ways of both in a growth chamber and in a greenhouse after seven days observation, and the growth characters such as leaf width/length, seedling width/length and fresh/dry weight, have been surveyed in a greenhouse for three months. Although in the growth chamber the highest germination rate was shown at a group with the kinetin treatment, it was not significantly different to that of the control group. Groups with ABA or NAA presented relatively low germination rates. As for the greenhouse test, the germination rates of all groups ranged 20~30% without significant difference each other, the reason of which might be due to low absorption by the effect of a soil drench method used in this study. The entire growth characters with the treatments of $2.15mg{\cdot}L^{-1}$ of kinetin and $10mg{\cdot}L^{-1}$ of $GA_3$ were significantly different to the control. NAA treatment only showed better growth of seedling width compared to the control. Consequently, the most effective PGRs for the germination and growth of mountain mulberry near the DMZ was kinetin. Further research on examining the most effective concentration of them was needed.

• Journal of Forest and Environmental Science
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• 제29권4호
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• pp.275-281
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• 2013

To establish in vitro nodal culture conditions of Echinosophora koreensis Nakai, one of rare and endangered species famous for beautiful flowers in the Korean Peninsula, the influence of plant growth regulators (PGRs) on shooting and rooting from in vitro shoots was investigated. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the half-strength Driver and Kuniyuki's media in the range of 2.22 to 8.88 ${\mu}M $induced 2.5 to 2.7 shoots per axillary bud; and addition of 2.27 ${\mu}M $ thidiazuron (TDZ) produced 3.2 shoots, during 4 weeks of culture, while zeatin and isopentenyl adenine (2ip) were not effective on shoot multiplication as observed from several combination treatments of BA with other PGRs. Shoots established were smaller than 2 cm in length, in most of the treatments. while in BA 8.88 ${\mu}M $ treatment more than 30% of shoots were longer than 2 cm and shorter than 4 cm. In rooting, naphthalene acetic acid (NAA) from 5.37 to 21.48 ${\mu}M $ showed the rooting rate from 40.0 to 62.5%. Indole butyric acid (IBA) addition had little effect on rooting (<10%), although some roots in IBA-containing media were longer than those in NAA. Micropropagation from axillary buds of nodular explants was applicable and promising to multiplication and conservation of Echinosophora koreensis Nakai.

• 원예과학기술지
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• 제29권3호
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• pp.260-266
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• 2011

An efficient protocol was established for high frequency somatic embryogenesis through a callus culture of Coelogyne cristata. The best frequency of callusing was obtained from a PLB segment (3-5 mm) cultured on MS medium supplemented with coconut water (CW) and a combination of both 3 $mg{\cdot}L^{-1}$ of 2,4-D and BA. When the calli were sub-cultured on the MS medium without any PGRs, the average number of somatic embryos were higher than those with PGRs treatment. NAA is the most critical factor among PGRs, which dramatically hindered for the formation of a somatic embryo. The efficacy of the addition of coconut powder (CP) for somatic embryogenesis was almost the same in all treatments. However, the number of somatic embryos formed distinctly depended on age of the callus. The somatic embryos converted into healthy plants with well-developed shoots on the same medium. Plantlets showed the best responses of root and shoot growth when transferred to $\frac{1}{2}$ MS medium containing 1.5 $g{\cdot}L^{-1}$ of activated charcoal. All plants with above 3.0-cm-high were successfully acclimatized in the greenhouse.

• 한국자원식물학회지
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• 제36권6호
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• pp.588-596
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• 2023

Long-term culture of cell lines is an important issue in in vitro culture and in plant science. In this study, the regeneration ability and ex vitro acclimatization of regenerants were evaluated. The ploidy level of regenerants derived from long-term cultured cell lines was measured in Imperata cylindrica 'Rubra', Poaceae. Adventitious buds (shoots) were successfully induced from five-year-cultured calli on MS medium containing 0.1 mg/L BA or 2.0 mg/L TDZ, combined with 0.01 mg/L auxins (IAA, IBA, NAA and 2,4-D), respectively. Adventitious roots were also induced on MS medium containing 0.01 mg/L auxins (IBA, NAA and 2,4-D), respectively. Interestingly, regenerants with both red and green leaf were successfully obtained when regenerants were cultured on MS medium with 9% sucrose. Regenerants derived from long-term cultured calli were transferred to pots using an optimal acclimatization process and successfully adapted to both pot and soil conditions. Moreover, the ploidy level was measured using calli and regenerants that had been kept on MS medium containing various kinds of plant growth regulators (PGRs).

• 한국조경학회지
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• 제28권3호
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• pp.1-12
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• 2000

식물생장조절제(Plant Growth Regulators, PGRs)를 이용한 예초관리 방법이 들잔디 엽색 및 품 질에 미치는 영향을 규명함으로 잔디 적응력 관점에서 적합한 PGR 종류 및 살포수준을 파 악하고자 본 연구를 시작하였다. 실험은 들잔디 생육이 왕성한 시기인 6월(실험 I)과 7월(실험 II) 2회에 걸쳐 각각 다른 장소에서 실시하였다. 공시약제 처리구는 Type I PGR인 amidochlor 3 수준 및 mefluidide 3 수준 과 Type II PGR인 trinexapacethyl 4 수준, 그리고 무처리구를 포함하여 전체 11 처리구였 고, 실험구 배 치는 난괴법 4 반복으로 하였다. 공시한 3종류 PGR 모두 잔디엽색 및 품질에 통계적으로 유의한 차이 가 있었지만, PGR 종류.살포수준 및 생육 상태에 따라 처리효과는 다르게 나타났다. 잔디 엽색 및 품질 은 PGR 살포후 경과기간에 따라 점진적으로 억제되는 경향이었다. 처리 1주후 잔디품질은 대조구에 비 해 큰 차이가 없었지만, 2주에서 4주까지는 PGR 종류 및 살포수준에 따라 유의하게 감소하 여 0.08 ml/ $m^2$ 이상의 trinexapac-ethyl 처리구 및 1.20 ml/$m^2$ 이상의 mefluidide 처리구에서는 가장 크 게 나타났다. 처리 4주부터 대부분 처리구에서 잔디품질이 적정수준 이상으로 회복되기 시작하였다. 본 실험을 통해 잔디관리 수준에서 저관리 잔디지역은 0.08 ml/$m^2$ 수준의 trinexapac-ethyl 처리구가 장기간 생장억제 관점에서 실용성과 경제성 측면에서 들잔디 관리에 효율적인 것으로 판단되었고, 고관리 잔 디지역은 0.04~0.08ml/$m^2$ 수준의 trinexapac-ethyl 처리구 및 0.60~1.20 ml/$m^2$ 수준의 amidochlor 처 리구가 적합 한 것으로 판단되었지만, 계절에 따라 PGR 효과정도가 다르게 나타났다. 따라서, 잔디 관리 시 잔디의 생육상태, 예초 절감 기간, 잔디품질의 기대수준 및 적용할 수 있는 관리수준에 따라 적합한 PGR 종류 선정과 함께 살포시기 및 살포량을 효율적으로 결정하는 것이 필요한 것으로 사료되었다.