Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of Plant Growth Regulators on Plant Regeneration Through Somatic Embryogenesis of Medicago sativa L.

  • Kim, Young-Sook (Division of Biological Sciences and the Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Kim, Mi-Young (Division of Biological Sciences and the Institute for Molecular Biology and Genetics, Chonbuk National University) ;
  • Yang, Moon-Sik (Division of Biological Sciences and the Research Center for Bioactive Materials, Chonbuk National University)
  • Published : 2004.06.01
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Abstract

An efficient plant regeneration system in alfalfa (Medicago sativa L.) through somatic embryogenesis was established. Embryogenic callus was obtained by culture of hypocotyl segments on MS medium with 0.02mg $L^{-1}$ IAA and 1.0mg $L^{-1}$ zeatin after 45 days of culture. Embryogenic calli were converted to the somatic embryos when transferred to either MS medium without plant growth regulators (PGRs) or MS medium containing various cytokinin (BA, kinetin and zeatin). Most of the somatic embryos were developed into plantlets on MS medium supplemented with 0.1 mg $L^{-1}$ kinetin. Also, secondary embryos appeared on the surface of primary embryo but they showed abnormal growth. Regenerated plantlets were transplanted to pots containing vermiculite and perlite for further analysis.

Keywords

References

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