• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.5
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• pp.584-590
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• 2012

Dried $Compositae$ flowers have traditionally been used for the treatment of anti-inflammatory and anti-oxidative stress in Korea. This paper investigates the effects of $Compositae$ extracts on the inhibition of proliferation and apoptosis of human gastric cancer AGS cells, human breast cancer MDA-MB-231 cells, and SK-BR-3 cells. The proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells were determined by MTT assay. Several $Compositae$ extracts inhibited proliferation of AGS cells, MDA-MB-231 cells, and SK-BR-3 cells in a dose-dependent manner. To assess the apoptosis of $Compositae$ extracts, the nuclei of MDA-MB-231 cells were stained with DAPI. The presence of chromatin condensation in the $Compositae$ extract-treated cells was detected on a fluorescent microscope (${\times}200$). We conducted Western blot analysis of changes in Bcl-2, Bax, and p53 protein expression levels. Apoptosis by $Chrysanthemum$ $zawadskii$ subsp. coreanum, $Chrysanthemum$ $zawadskii$ var. $tenuisectum$ and $Rudbeckia$ $laciniata$ var. $hortensis$ treatment created a decrease in Bcl-2 expression, whereas the expression of Bax and p53 were increased. These results indicate that $Chrysanthemum$ $zawadskii$ $subsp.$ $coreanum$, $Chrysanthemum$ $zawadskii$ var. $tenuisectum$ and $Rudbeckia$ $laciniata$ var. $hortensis$ inhibit breast cancer cell growth through the induction of apoptosis.

• Journal of the Korean Institute of Landscape Architecture
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• v.47 no.6
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• pp.12-23
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• 2019

The purpose of this study was to develop and study an experiential natural education program that could emphasize the importance of the natural environment by providing natural experience opportunities using the natural resources of the urban forest parks using Youngheung Park in Suwon, Gyeonggi Province as a target site. The research target was limited to Suwon Yeongheung Park, which had the potential to become a place for education, where urban forest conservation and sustainable use already coexist. The natural education resources derived by surveying and analyzing the basic environment and the ecology of plants and animals in Suwon Yeongheung Park were organized to establish program goals, directions, and themes. Suwon Yeongheung Park is a water-rich forest that forms an ecological system of wetlands, including rice paddies, muknon wetlands, and dungbun, near a valley area. The U-shaped walkway was smoothly formed along the ridge and includes Doran-gil, which is among the Palochrome Road, designated by the city of Suwon. The soil is acidic, with a pH 4.40, due to urban pollution and acid rain, and is not good for plant growth. Most of the artificial forests, natural forests, and arable land were found using land use and extant life surveys. Old trees were distributed in artificial forests, the oak clusters in natural forests, and the fields and darrinones were distributed in the arable areas. As the forest vegetation declined, the cedar forest was underway, and the cedar trees and red bean pear trees were cultivated due to their adaptability to the urban environment. There are 13 large of 180 sacks, one being 109 centimeters in diameter, the largest silvery tree, and 105 oak trees, provide food and shelter for animals. Six species of waterfowl that used the 22 kinds of forest wetlands, while four species of amphibians and two species of reptiles reside in the wetlands. Natural Monument No. 327, Mauryuk, Class II Endangered Wildlife, was also observed in the wetlands. Eight other species of surface dragonflies and three species of butterflies were observed. By systemizing the resources, members, and characteristics of the forest ecosystems in Suwon Yeongheung Park based on five criteria, the program for a hands-on natural education was presented with the aim of understanding the urban forest ecosystem in Suwon Yeongheung Park, having an affinity with the city, and recognizing its relationship with the community and society. However, further research is needed as there are limitations of research on programs characterized by different ages and classes.

• Korean journal of applied entomology
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• v.47 no.1
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• pp.75-86
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• 2008

Many kinds of environment friendly agricultural materials (EFAMs) were used for the plant protection, furthermore they support the growth of crops in the greenhouses and the kindly environment friendly farming. Natural enemies might be used for control of insect pest with EFAMs at the same space and time. For testing the toxicity of EFAMs against to natural enemies, 10 EFAMs were selected and tested in small greenhouses and farm's fields. In case of Harmonia axyridis larva, there was no predacious activity against cotton aphids in the block with EFAMMo L, EFAMPE D, EFAMME G and EFAMAE A, otherwise, aphid population was rapidly decreased in the control block without EFAMs. Both of Trialeurodes vaporariorum and Encarsia formosa population were slightly decreased in the block with EFAMs. There were significantly decreased in the block with IEFAM C, FEFAM D, EFAMSM Band EFAMPE D compared with control area. Especially, there was significantly maintained a very low population, both T. vaporariorum and E. formosa in the block with EFAMMo C. The parasitized ratio of Aphidius colemani is also very low in the block with EFAMs compared with control area. In the block with IEFAM C, FEFAM D, EFAMPE D and EFAMMo L, there were significantly different with control area in the number of mummies. Otherwise, in case of Diglyphus isaea and Liriomyza trifolii, there was no significantly different between blocks with and without EFAMs. In the block with EFAMMo C, Tetranychus urticae and Phytoseiulus persimilis populations were significantly maintained a very low level. However, the population of T. urticae was increased, but P. persimilis was decreased in the block with EFAMMo L.

• Journal of Mushroom
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• v.3 no.3
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• pp.109-114
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• 2005

Attempts were made to control Diatrype stigma occurred on the bed-log of shiitake by resistant shiitake strains. In selection test of resistant shiitake strains, 67 out of 77 strains tested were proved to be resistant to D. stigma. Among them, 13 strains including KFRI 5 were effective to inhibit the access of D. stigma, and 7 strains including KFRI 180 remarkably invaded the territory of D. stigma. Among 31 shiitake strains made by hybridization of resistant strains for D. stigma, 8 strains including KFRI 537 inhibited the access of D. stigma, and 4 strains including KFRI 545 invaded the territory of D. stigma. The effects of temperatures and inoculation orders to the resistance were confirmed in PDA plates and test tubes filled with sawdust of Quercus acutissima. Four kinds of temperature treatments as follows were tested: (1) continuous incubation at $14^{\circ}C$, (2) continuous incubation at $25^{\circ}C$, (3) changing of incubation temperature from $14^{\circ}C$ to $25^{\circ}C$ as soon as mycelia of both shiitake and D. stigma meet together, (4) changing of incubation temperature from $25^{\circ}C$ to $14^{\circ}C$ as soon as mycelia of both shiitake and D. stigma meet together. Three kinds of inoculation procedure were tested: (1) inoculation of shiitake 3 days ahead of D. stigma inoculation, (2) inoculation of D. stigma 3 days ahead of shiitake inoculation, (3) simultaneous inoculation of both fungi. In PDA plate test, the strain KFRI 137 showed outstanding ability to inhibit mycelial growth of D. stigma and the strain KFRI 180 invaded into the territory of D. stigma in most of treatments. Hybrid strains, KFRI 545, 546, and 547 were more resistant than their parent strains, KFRI 488 and 405. In test tube examinations, all the strains of shiitake showed high resistance at the treatment of change in temperature from $14^{\circ}C$ to $25^{\circ}C$ when mycelia of both shiitake and D. stigma meet together. On the other hand, resistance of all the strains growing at $25^{\circ}C$ decreased when the temperature was changed into $14^{\circ}C$ after mycelia of both fungi. In these cases, the resistance reached to 7~20% of the highest resistance. The strain KFRI 259 invaded the territory of D. stigma, contrary to PDA plate test. Among the strains, KFRI 393 strain was the most resistant under the continuous incubation at $25^{\circ}C$.

• Korean Journal of Ecology and Environment
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• v.46 no.2
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• pp.276-288
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• 2013

The present study investigates the effects of elevated soil nitrogen on growth and decomposition of Oryza sativa shoots. The plants were cultivated in greenhouse until leaf senescence and the total biomass of the plant increased 1.9 times at nitrogen addition plot. Total C and N content in shoot increased; however, lignin, C/N, and lignin/N levels decreased in the N-treated soil. The shoot litters collected from the control and N-treated soil were tested for decay and microbial biomass, $CO_2$ evolution, and enzyme activities during decomposition on the control and N-treated soil at $25^{\circ}C$ microcosm. The remaining mass of the shoot litter was approximately 6% higher in the litter collected from the control soil (53.0%) than the litter collected from high N-treated soil (47.1%). However, the high N-containing litter exhibited faster decay in the control soil than in the N-treated soil. The litter containing high N, low C/N, and low lignin/N showed a higher decomposition rate than that of low quality litter. The N-addition showed decreased microbial biomass C and dehydrogenase activity in soil; however, it exhibited high microbial biomass N and urease activity in soil. When the high N-containing litter decays on the N-treated soil, the microbial biomass C increased rapidly at the initial phase of decomposition and decreased thereafter, and dehydrogenase activity was less that of other treatment; however, there was no effect on the microbial biomass N. The urease in the decomposing litter was highest during the early decomposition stage and dramatically decreased thereafter. The present findings suggested that the N-addition increased N content in litter, but inhibited the decomposition process of above-ground biomass in terrestrial ecosystems.

• Asian Journal of Turfgrass Science
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• v.23 no.1
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• pp.143-150
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• 2009

High salt concentration is one of the most important limit factor on plant growth at a disused saltpan for golf course construction site. The control of salt in soil is definitely required and the monitoring of salt concentration in soil and ground water also required to amend soil physiochemical properties. This research was carried out to monitor the pH and salt concentration changes by the height of ground water. By the physiochemical analysis test, the soil contains a high salt concentration and classified as a slight alkaline clay soil. The height of ground water table changed to 1.3m, 3.3m and 2.8m at dry season(mid-late June, 2005), monsoon season(early-mid July) and after monsoon(late July), respectively. Compare to the average ground level of 2.9m, the ground water was over flooded about OAm at monsoon season. The electrical conductivity(ECe) was measured above $4.0dS{\cdot}m^{-1}$ over all areas and however, some areas showed over $20dS{\cdot}m^{-1}$. During a monsoon season, ECe was lowered to $1.2{\sim}15.0dS{\cdot}m^{-1}$, compared with those of the dry season. Therefore, the interception of the capillary connection between planting layer and ground water which contains high salt concentration should be adapted when golf courses are constructed on disused saltpan. The phytotoxicity caused by salt damage may be controled by the interception of capillary fringe of salt flow to the topsoil profile at the upper layer of the ground water table.

• Korean Journal of Agricultural Science
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• v.15 no.2
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• pp.123-131
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• 1988

This study was done to investigate the interplanting effects of corn, when a corn hybrid with tillers (IK derivaties) was interplanted with a corn hybrid without tillers(Jinjoo Ok). The interplanting ratios of two hybrids, IK and Jinjoo Ok, were 1 to 1, 1 to 2, 1 to 3, 2 to 1, and 3 to 1. The interp1anting effects were compared with monoculture for major agronomic characteristics. 1. The plant height was increased when interplanted regardless of the interplanting ratios of the two hybrids. The general growth of IK hybrid was a little hindered when the interplanting ratio of Jinjoo Ok hybrid increased. 2. The number of tillers produced by the IK hybrid increased until 40 days after emergence and appeared to increase as the interplanting ratio of IK hybrid increased. 3. When monocultured, the fresh weight of IK hybrid per 10a was 8,096kg and and 6,677kg at silking and dough stages, respectively and these were 77%, 53% over the fresh weight of Jinjoo Ok at the sarne stages. When IK and Jinjoo Ok hybrids were interplanted in 1 to 1 ratio, the fresh weight of IK was 20% higher than that of Jinjoo Ok monocultured. 4. The highest grain yield per 10a was obtained from the Jinjoo Ok hybrid monocultured and the yield was 434kg. Even the interplanting ratios between IK and Jinjoo Ok were 1 to 1 and 1 to 3, the grain yield of Jinjoo Ok was 16% and 13% over the IK, respectively. 5. Root and stalk lodging of IK hybrid was 29% when monocultured and seemed to increase as the interplanting ratio of Jinjoo Ok increased. The lowest lodging was found when the interplanting ratio of IK and Jinjoo Ok was 1 to 1. 6. Stem diameter of IK hybrid was also decreased as the interplanting ratio of Jinjoo Ok increased. The ear height of IK was lowered as the interplanting ratio of IK was increased. The two hybrids tested seemed to have tolerance to the black streaked dwarf virus(BSDV).

• Korean Journal of Agricultural Science
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• v.9 no.2
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• pp.467-483
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• 1982

Leaflets of Allium sativum L. (garlic) and leaf scales Allium cepa L. (onion) were cultured on the Murashige and Skoog(MS) medium to study the effects of plant growth regulators, temperature and light on the formation of shoot and root. And also, the potentiality of shoot or root formation of tissues in accordance with the leaf age and the region was discussed. In the experiment with garlic, the formation of shoot and root was more effective on the medium containing $BA\;2mg/{\ell}$ and $NAA\;1mg/{\ell}$ and it occured on the basal region of outer leaflets. The shoot formation was more effective at $20^{\circ}C$ than $28^{\circ}C$ under 16-hour daylength, and it was more effective in darkness than light condition at $28^{\circ}C$. The results of shoot and root formation on the new cloves of garlic harvested in 1982 were similar to those the old cloves of garlic harvested in 1981. In the experiment with leaf seales of onion, when the tissues of dormant leaf scales of onions harvested in 1981 were explanted on the MS medium, a few shoots were formed on the medium containing $BA\;2mg/{\ell}$ and $NAA\;1mg/{\ell}$. In this case,there were no differences on the shoot formation among the age and region of leaflets. On the culture of leaf scales, the shoot was not formed on the leaf scales located at outer or middle region, but it was formed on the medium containing BA accompanied with NAA at inner scales. On the medium complemented with BA alone, the tissues were not differentiated, but on the medium complemented with NAA, the callus was formed from tissues and then the root was formed through the callus.

• Korean Journal of Environment and Ecology
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• v.18 no.4
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• pp.369-381
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• 2004

This study was achieved to present the way to restore the Seonheulgot pasture-grassland damaged by landuse and interference for a long time to evergreen broad-leaved forests as the native vegetation structure. As a result of analyzing ecological succession tendency of structure in survey area, we established the optimal restoration model. The total of survey sites were 26, and the classified plant community types were four types by M.I.P of dominant woody species. Finally we classified the four types based on diameter of dominant woody species in canopy layer. The six community types are as follows: Community I was runner-shrub forest, community II was evergreen broad-leaved shrub forest, and community III was evergreen broad-leaved forest of small diameter. Community IV and V were evergreen broad-leaved forest of middle diameter. Community Ⅵ was evergreen broad-leaved forest of large diameter. The number of constituent species was 24 in community I, 28 in community II as the shrub forest, 16 as the evergreen broad-leaved forest of small diameter, 29 in community III, 30 in community IV as the evergreen broad-leaved forest of middle diameter and 27 in community Ⅵ as the evergreen broad-leaved forest of large diameter. The range of Shannon's index of all communitys was from 0.8763 to 1.2630 and the Similarity index between the community composed of middle diameter woody species and large diameter woody species. The ecological succession of community I, II, and III were changed from pasture-grassland to broad-leaved forest and the structure of community IV, V, and Ⅵ was similar to evergreen broad-leaved forest in warm temperate region. We suggest the restoration planting model evergreen broad-leaved forest of in Seonheulgot pasture-grassland, as follows: The target restoration vegetation were Castanopsis cuspidata var. sievoldii community and Queycus glauca community. Castanopsis cuspidata var. sievoldii and Quercus glauca should be dominant woody species in canopy layer, the number of trees was 10 per 100$m^2$, and Castanopsis cuspidata var, sievoldii, Quercus glauca, Camellia japonica, and Eurya japonica should be dominant woody species in the understory layer, the number of trees was 14 per 100$m^2$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.6
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• pp.747-756
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• 1995

To find out the optimum application method of slow-releasing fertilizer(SRF) and conventional fertilizer(CF) with different fertilization rate under two culture methods[l0-day old seedling machine transplanting(MT) and direct-sowing on dry paddy(DS)] in the south-western region(clay loam soil) of Korea, used were Chosun slow-releasing fertilizer(silicate latex coated fertilizer: N-P$_2$O$_{5}$-K$_2$O =18-12-13) and conventional fertilizer. Plant height and number of tillers with different two culture methods were higher at MT than DS in early growth. The ratio of dry weight in heading stage was higher at CF than SRF in MT than DS and especially, SRF 80% + CF 20% than SRF 100% or CF 100%. Leaf area index (LAI) in heading stage was higher at CF in MT but higher at SRF in DS than their counterparts. Chlorophyll content was higher at SRF than in CF expect for heading stage(HS), especially in DS. It was highest at HS in CF without its difference during maximum tillering stage(MTS) and panicle formation stage(PFS), while highest at PFS in SRF with tendency of gradual increase and decrease before and after PFS, respectively. Heading was delayed 2~3 days at SRF in two cultrue methods and 4~5 days at SRF in DS in comparison with CF in MT with delay of 2 days at DS compared with MT. Culm length was longer at CF in MT and at SRF in DS than their counterparts. Panicle number per m was more at SRF and in DS. Filled grain ratio was higher at CF and in MT. Yield was obtained 101 and 100% at 100% and 80% level of SRF in DS respectively, and 96% at 80% level of CF in MT, compared with conventional application method (516kg /l0a), and increased 2~4% at DS and 0~3% at MT in SRF. Yield was high in order of 100%(SRF) =80%(SRF) + 20%(CF) > 100%(SRF) + 20%(CF) > 80%(SRF) at MT and 80%(SRF) + 20%(CF) =100%(SRF) > 80%(SRF) =100%(SRF) + 20%(CF) at DS.