• The Korean Journal of Pesticide Science
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• v.20 no.1
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• pp.41-46
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• 2016

Bacterial fruit blotch (BFB) caused by Acidovorax avenae subsp. citrulli is defective disease to watermelon cultivated areas. To control of BFB, we investigated control efficiency to use commercial antibacterial pesticides. Growth inhibition zone on medium were formed as oxolinic acid WP and oxytetracycline WP. Control efficacy of four anti-bacterial pesticides on seed and seedling stage were performed. As a results, oxytetracyclin WP is shown over 90% control efficiency on seed and acibenzolar-S-methyl + mancozeb WP shown over 90% control efficiency on seedling stage Hot-water treatment method could be possible to reduced infection rate on seed. The conditions of hot-water treatments are $50{\sim}55^{\circ}C$ on 20~30 minutes. These results suggested that the methods were helpful watermelon seedling nursery to control of the bacterial fruit blotch by A. avenae subsp. citrulli.

• Nutrition Research and Practice
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• v.14 no.4
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• pp.322-333
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• 2020

BACKGROUND/OBJECTIVES: Arterial stiffness and endothelial dysfunction are 2 of the independent predictors for cardiovascular disease, while Acanthopanax senticosus Harms (ASH) is a traditional medicinal plant that can improve cardiovascular health. This study aimed to investigate the efficacy of the fruit of ASH on vascular function in apparently healthy subjects. SUBJECTS/METHODS: A 12-week, randomized, double-blind, placebo-controlled design, consisting of healthy adults with at least 2 of the following 3 conditions: borderline high blood pressure (BP; 120 mmHg ≤ systolic BP ≤ 160 mmHg or 80 mmHg ≤ diastolic BP ≤ 100 mmHg), smoking (≥10 cigarettes/day), and borderline blood lipid levels (220 ≤ total cholesterol ≤ 240, 130 ≤ low density lipoprotein cholesterol ≤ 165, or 150 ≤ triglyceride ≤ 220 mg/dL). Randomly assigned 76 subjects who received a placebo or 2 doses of ASH fruit (low, 500 mg/day; high, 1,000 mg/day) completed the intervention. Brachial-ankle pulse wave velocity (baPWV), flow-mediated dilation, carotid intima-media thickness, and BP were measured both at baseline and following the 12-week intervention. Endothelial nitric oxide synthase (eNOS) phosphorylation was assessed by western blotting. RESULTS: Compared with the placebo group, the low-dose group showed more significant changes after the 12-week intervention period in terms of systolic BP (0.1 vs. -7.7 mmHg; P = 0.044), baPWV (31.3 vs. -98.7 cm/s; P = 0.007), and the ratio of phospho-eNOS/eNOS (0.8 vs. 1.22; P = 0.037). CONCLUSIONS: These results suggest that ASH fruit extract at 500 mg/day has the potential to improve BP and arterial stiffness via endothelial eNOS activation in healthy adults with smoking and the tendency of having elevated BP or blood lipid parameters.

• The Korean Journal of Mycology
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• v.45 no.4
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• pp.386-393
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• 2017

Onion downy mildew, caused by Peronospora destructor, is a major disease in onion cultivation areas in Korea. The causal fungi were collected and analyzed based on sequence similarity and molecular phylogenetic relationships of multi-gene sequences, including the internal transcribed spacer (ITS) region. All isolates from Changnyeong-gun, Hamyang-gun, and Hapcheon-gun in Gyeongnam province, and Muan-gun, Haenam-gun, and Sinan-gun in Jeonnam province were identical in the four types of gene sequences, indicating they were genetically the same strains. In this study, a PCR method was developed based on the ITS gene sequences to amplify the specific DNA fragment for P. destructor only. The detection limit of was total genomic DNA of the P. destructor and the plant $0.7ng/{\mu}L$. Therefore, the developed PCR method could be used to detect P. destructor effectively from symptomless onion leaves.

• Journal of Veterinary Clinics
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• v.26 no.1
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• pp.1-7
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• 2009

The Artemisia capillaris THUNB is a perennial herb that belongs to the family Compositae spp and probably the most common plant among the various herbal folk remedies being used in the treatment of abdominal pain, hepatitis, chronic liver disease, jaundice and coughing in Korea. Recently the biological and pharmacological actions of herb have been studied well such as antibacterial, antidiabetic and antitumor activities. This experiment was conducted to investigate antitumor and immunomodulatory effects of Artemisia capillaris extracts against Hepa-1c1c7 and Sarcoma 180 cancer cells in in vivo experimental tests. In in vivo experimental tests using 210 ICR mice, based on flow cytometry, CD3+, CD4+, CD8+ and TNF-${\alpha}+$ splenocytes were significantly (p<0.05) reduced in the Hepa-1c1c7 and Sarcoma 180 inoculated vehicle controls, HP and SP, compared to those of the intact vehicle control on both the $28^{th}$ day and the $42^{nd}$ day, respectively. These decreases of CD3+, CD4+, CD8+ and TNF-${\alpha}+$ cells induced by tumor inoculations were significantly (p<0.05) inhibited by mACH treatment regardless of the type of experiments and tumor cells inoculated. The results suggest that Artemisia capillaris methanol extracts have prominent antitumor effects on the cancer cell lines Hepa-1c1c7 and Sarcoma 180.

• Korean journal of applied entomology
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• v.26 no.1 s.70
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• pp.43-47
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• 1987

Effect of percent penetration and hyphal growth on lesion formation was evaluated at 23/15C (day/night), 29/21C regimes and greenhouse condition (33-23C) using three rice cultivars, Aichi-asahi, Toyotama and Yamabiko with 6 isolates of Pyricularia oryzae. Percent penetration at 72hr and 96hr after inoculation was highly significant with lesion formation at any temperature regime. At the same time, number of appressoria that exceeded hyphal growth value 11 was also highly significant with lesion formation. However, number of appressoria that exceeded hyphal growth value 30 was significant only at 72hr after inoculation at 29/21C regime. In general, statistical fitness of the model was higher at 23/15C regime than at 29/21C regime or a greenhouse condition. These series of phenomenon were more apparent in a susceptible cultivar Aichi-asahi.

• Asian Journal of Turfgrass Science
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• v.26 no.1
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• pp.17-23
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• 2012

Large patch caused by Rhizoctonia solani AG2-2 (IV) is one of the most serious diseases in zoysiagrass. The objectives of this research were to evaluate the in vitro fungicidal effect and in field control of large patch disease of Paraffin oil. In the field experiments, paraffin oil was applied with 1 L/$m^2$ after diluted at 5 ml to 20 ml of oil/1 L of water for the control of large patch on Zoysia japonica in golf course. The same material was tested for inhibition of mycelial growth in vitro. Paraffin oil at 0.5% and 2.0% had a fungicidal effect by 43 to 67% on R. solani AG2-2 (IV) in vitro. In two locations of the field experiments, the paraffin oil provided moderate protection of large patch. It was effective in suppressing large patch development by 48.0% in comparison with a water check after one time application on mid-September in golf course. Paraffin oil may be used as an alternative control agent for environment friendly management of large patch on Zoysiagrass in golf course.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.20 no.1
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• pp.47-52
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• 2010

Green tobacco sickness (GTS) is known as an occupational disease among tobacco harvesters, and a form of acute nicotine intoxication by the absorption of nicotine through the skin from the wet green tobacco plant. On the assumption that GTS may occur by inhalation as well as absorption of nicotine, we measured the airborne nicotine concentration in tobacco field and the processing room of tobacco leaves. We measured the airborne nicotine concentrations in the tobacco field and processing room between 13 and 30 July 2008. All sampling and analyses of airborne nicotine were conducted according to the manual of analytic methods of NIOSH 2551, and we sampled 2 times at 11 points in the tobacco field by area sampling. The sampling in the processing room of tobacco leaves was conducted at 3 points, and earlymorning dew was collected from the tobacco by wringing the moisture into specimen bottles. The airborne nicotine concentration [geometric mean (geometric standard deviation)] in the tobacco field in the P.M. was higher [49.2 mg/$m^3$ (1.3)] than the A.M. concentration [43.4 mg/$m^3$ (1.4)]. Similarly, the nicotine concentration in the processing room of tobacco leaves was 224.4 mg/$m^3$ (1.2), and the concentration of nicotine in the dew was 64.7 mg/${\ell}$ (1.7). Based on our results, the airborne nicotine concentration in the tobacco field and the processing room of tobacco leaves were 100 and 400 times higher than the occupational recommended values (TLV-TWA of 0.5 mg/$m^3$), respectively. In the future, it is hoped that epidemiologic studies and environmental measurements will be conducted for GTS which occurs by inhalation of nicotine. If GTS is confirmed to occur by inhalation of nicotine, respiratory and dermal protective equipment must be distributed.

• Asian Journal of Turfgrass Science
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• v.25 no.2
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• pp.133-137
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• 2011

Winterkill can be defined as any injury including freeze stress kill, winter desiccation, and low temperature disease to turfgrass plants that occurs during the winter period. The major damages from winterkill were low temperature kill, crown hydration, and winter desiccation. Low temperature kill is caused by air and soil temperature. Soil temperature affect more severe to turfgrass than air temperature because low soil temperature cause fetal damage to turfgrass crown. Crown hydration is a form of winter injury in which intercellular water within the plant freezes and causes physical injury to the cell membrane and wall. This is eventually resulted in dehydration of cell. Winter desiccation is the death of leaves or whole plants due to drought during the winter period. To reduce winterkill damage, cultivar selection is very important. If changing cultivar is not allowed, cold temperature hardiness needs to be increased by providing nutrients especially phosphorus and potassium in the late fall. Turf cover is effective way to reduce winterkill damage. Remaining snow is positive process to reduce winterkill damage by insulating soil temperature. The previous researches reported many materials as turf cover such as straw, polypropylene, polyester, and wood mat. Aeration and topdressing is one of the process against winterkill. Both methods are mainly conducted to reduce thickness of thatch layer. In recent, relatively new materials called black or winter topdressing sand are used to protect soil temperature from low air temperature and thaw ice crystal that may remain in soil.

• Journal of Ginseng Research
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• v.32 no.4
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• pp.275-278
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• 2008

Ginseng stem fungus gnat, Phytosciara procera, is reported as a ginseng pest for the first time. It is new to science that a member of the family Sciaridae is a ginseng pest. In our observation, larvae of the gnat can penetrate the stem of ginseng, and then they make a shaft in shoot and root. Number of adults captured by yellow sticky traps were peaked in twice, from late July to early August and from late August to early September. In a ginseng field, 29.7% of ginseng damaged by Phytosciara procera is also infected by bacterial disease caused by Erwinia carotobora. However, there is a possibility on environmental-friendly control, as a result of decreasing effect of damage over 85% when remaining a part of berry on peduncle than tatally remove.

• Journal of Nutrition and Health
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• v.42 no.2
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• pp.146-157
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• 2009

Korean diet is high in plant foods but also high in salted vegetables. World Cancer Research Fund (WCRF) recommends consumption of fruits and vegetables excluding salted vegetables for prevention cancer. This study aimed to analyze relations between intakes of salted and non-salted vegetables and socioeconomic factors, providing a data for targeted groups in promotion of fruits and vegetables consumption. Dietary and socioeconomic status data of the 5,400 subjects over 30 years of age from the 2005 Korea National Health and Nutrition Examination Survey (KNHANESIII) were used. Dietary intake data from KNHANESIII was obtained by one day 24-hour recall method. Mean daily intakes of salted vegetables, non-salted vegetables and fruits of subjects were 151 g, 237 g, and 71 g respectively. Mean daily intake of salted vegetables was significantly higher in men than women for daily amount (173 g vs. 133 g) as well as percentage of total food intake (9.9% vs. 9.6%). Subjects living in rural area consumed more salted vegetables. Salted vegetables as percent of total food were lower in subjects with higher education levels (p < 0.001). Intakes of non-salted vegetables were significantly affected by age and gender. Intake levels of fruit were significantly higher in younger groups, in females, and subjects with higher income and education levels (p < 0.05). Average intake of fruits and non-salted vegetables was 307 g, lower than WCRF recommended level of 400 g for personal guideline. Intake of salted vegetables was positively correlated with sodium intake (Pearson's correlation coefficient, r = 0.43) but less so with potassium (r = 0.16) and other micronutrients intake (r < 0.1). On the other hand, non-salted vegetables had higher correlations with potassium (r = 0.45), carotene (r = 0.38), vitamin A (r = 0.37), iron (r = 0.34) and low for sodium (r = 0.13). Fruits intake was highly correlated with vitamin C intake (r = 0.46). Proportion of subjects satisfying WCRF personal guideline of fruits and non-salted vegetables was 25.7%. Results of this study indicate that intake of salted vegetable is considerably high among Koreans, and it is highly correlated with sodium intake and less so with other micronutrients.