• 대한본초학회지
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• 제21권1호
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• pp.57-62
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• 2006

Objectives : This study was carried out to investigate the inhibitive effects of cotton plant sectional extracts in cancer cell lines, Calu-6(human, Caucasian, lung, adenocarcinoma) and MCF-7(human, Caucasian, breast, adenocarcinoma). The incidence of cancer has been increasing even in korea due to the change of dietary life and westernization and becoming conspicuous as the disease threatening health. But cancer treatment have not been fully effective against the high incidence or low survival rate of most cancer. Methods : Calu-6 and MCF-7 cells were cultured and seeded in cell culture plates, respectively. And sectional extracts of cotton plant were treated to MCF-7 cells. Results and Conclusion : Sectional extracts of cotton plant showed no anti-proliferative effect on MCF-7 cells, but root and stem extracts showed strong anti-proliferative effects on Calu-6 cells. Fruit, leaf and flower extracts also showed anti-proliferative effects on Calu-6 cells but not so much like root and stem extracts. But seed extract showed no anti-proliferative effect on Calu-6 cells.

• 한국미생물·생명공학회지
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• 제44권3호
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• pp.383-390
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• 2016

본 연구는 대식세포에서 LPS를 이용하여 염증 유사 세포 모델을 만들고 염증 유사 대식세포 모델에서 성체줄기세포의 면역 조절 능력을 평가하였다. LPS 자극에 의해 증가된 IL-1β, TNF-α 및 IL-10의 생성은 성체줄기세포를 공배양한 실험군 뿐만 아니라 성체줄기세포를 배양한 상층 배양액을 처리한 실험군에서도 동일한 효과를 나타내었으며, 또한 성체줄기세포 유래 엑소좀을 염증 유사 대식세포 모델에 처리하여 유사한 결과를 관찰하였다. 이 결과는 성체줄기세포 자체의 염증 억제 기능보다는 성체줄기세포 유래 엑소좀을 포함하여 성체줄기세포가 분비하는 bioactive molecules에 의해 세포 간 신호 전달이 이루어지고 있음을 의미하며, 이러한 엑소좀은 염증 관련 질환 분야에 치료적 적용이 가능하고 또한 새로운 염증 치료제 개발의 툴로 사용될 수 있음을 시사한다.

• 한국유기농업학회지
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• 제6권1호
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• pp.151-160
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• 1997

Azospirillum sp., photosynthetic bacteria(Rhodopseudomonas sp.) and Pseudomonas sp. were separated and screened from soil and soilless culture, and identificated. The antifungal activities against root-infected pathogens and plant growth promoting effects of the cultured solution of the starins(5.0$\times$105 cells/$m\ell$) in the peatmoss compost on the early growth of cucumber and tomato seedling were investigated. Azospirillum sp. and Pseudomonas sp. showed a antifungal activities against Fusarium sp., Pythium sp. and Rhizoctonia sp in thed ranges of 51.0% to 72.0% on potato dextrose agar medium, however photosynthetic bacteria had not antifungal activities. When cultured solution of Azospirillum sp., photosynthetic bacterial and Pseudomonas sp. were bacterialized by mixing with peatmoss compost, early growth of cucumber and tomato in terms of plant height, number of leaves, leaf area, root length, fresh anf dry weight of leaf, stem and root were promoted, especially photosynthetic bacteria had a the best plant growth promting activities.

• 한국자원식물학회지
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• 제23권3호
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• pp.207-213
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• 2010

Hovenia dulcis Thunb fruits were successively extracted with hot water, water, methanol, ethyl acetate, and chloroform. The crude extracts were investigated for potential antioxidant by measuring scavenging against DPPH free radicals, reducing power, superoxide radicals, and protection of protein damage and cultured cells from a lethal dose of hydrogen peroxide ($H_2O_2$). In all chemical assays used, the hot water extract of H. dulcis fruits, which contained $61.14{\pm}2.57$ (Tannic acid mg/g extract, n=3) of total phenolic compounds contents exhibited highest activity in in vitro models of DPPH free radical scavenging activity, reducing power assay, superoxide radical scavenging activity and protection of protein damage. In addition, the hot water extract protected cultured RAW 264.7 macrophages from a lethal dose of $H_2O_2$ and reduced reactive oxygen species level in RAW 264.7 cells.

• 식물조직배양학회지
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• 제26권3호
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• pp.183-187
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• 1999

식물배양세포를 이용하여 효율적인 제초제 검정시스템을 확립하기 위하여 다른 작용기작을 가진 몇가지 제초제를 사용하여 담배 PM세포에 대한 반응성을 세포생장과 배지의 이온전도도를 측정하여 조사하였다. 담배 PM세포는 0.7 mg/L 2,4-D, 0.3 mg/L kinetin, 30 g/L sucrose를 함유한 MS배지에서 $25^{\circ}C$, 광조건에서 현탁배양 (100rpm)하였다 계대배양시 약제를 처리한 후 12일째의 세포생장과 배지의 이온전도도를 측정한 결과, 이온전도도 측정결과는 세포생장의 것과 높은 상관관계를 나타내었다. 담배 PM세포에 대한 각 화합물의 반응성은 약제의 작용기작을 반영하면서 다양하였다. PET 저해화합물인 atrazine은 담배 PM세포에 가장 강한 활성을 나타내었다 (IC50, 1 $\mu$M). GS 저해제인 glufosinate, 세포분열저해제인 butachlor, carotenoid 생합성저해제인 fluridone은 농도에 비례하여 저해활성을 나타내었다. 그러나 오옥신활성을 지닌다고 알려진 quinclorac은 억제활성을 나타내지 않았다. 따라서 배지의 이온 전도도 측정은 간편하면서 재현성이 좋은 신규 제초제의 탐색방법으로 유용할 것으로 시사된다.

• Natural Product Sciences
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• 제8권4호
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• pp.170-172
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• 2002

In continuous efforts for discovery of novel potent antitumor agents from natural products, fifty-seven methanolic extracts derived from indigenous Korean plants were primarily evaluated for in vitro cytotoxic activity in cultured human lung (A549) and colon (Col2) cancer cells. As a result, 16 plant extracts were found to be active against A549 cells and 15 extracts were active against Col2 cells in the criteria of $IC_{50}$<$50\;{\mu}g/ml$. In particular, the extracts of Calystegia soldanella $(IC_{50}$<$8.0\;{\mu}g/ml\;in\;A549;IC_{50}=27.4\;{\mu}g/ml\;in\;Col2)$, Heloniopsis orientalis $(IC_{50}=4.6\;{\mu}g/ml\;in\;A549; IC_{50}=4.5\;{\mu}g/ml\;in\;Col2)$, and Thuja koraiensis $(IC_{50}=1.2\;{\mu}g/ml\;in\;A549;IC_{50}=0.6\;{\mu}g/ml\;in\;Col2)$ showed a potent cytotoxic activity. Further study for the identification of active compounds from these lead extracts might be warranted.

• 환경위생공학
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• 제24권2호
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• pp.40-48
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• 2009

Unusual bloom of toxic cyanobacteria in water bodies have drawn attention of environmentalists world over. Major bloom of Anabaena, Microcystis in water storage reservoir, rivers and lake leading to adverse health effects have been reported from Australia, England and many part of the world. These cyanobacterial cells can release intercellular matter like toxin in water and these intercellular matter can increase the concentration of organic matter. Cellysis can occur when algal cells meet the disinfectants like chlorine in water treatment plant and the resultant rising up of DOC(Dissolved Organic Carbon) or TOC(Total Organic Carbon) can increase the formation of disinfection by products. Disinfectants that kill microorganisms react with the organic or inorganic matter in raw water. In general disinfectants oxidize the matter in raw water and the resultant products can be harmful to human. There are always conflict about which is more important, disinfection or minimizing disinfection by products. The best treatment process for raw water is the process of the lowest disinfection by products and also the the lowest microorganism. In this study the cultured cells, Microcytis Aeruginosa(MA), Anabaena Flos-aquae(AF), Anabaena Cylindrica(AC), and the cells obtained in Daechung Dam(DC) whose dominant species was Anabaena Cylindrica were subjected to chlorination. Chlorination oxidizes inorganic and organic compounds and destruct live cells in raw water. Chloroform was analyzed for the cultured cells which were treated with $20mg/\ell$ dose of chlorine. In general chloroform is easily formed when dissolved organic matter react with chlorine. The cultured cells contributes the concentration of dissolved organic carbon and also that of total organic carbon which might be potent precusors of chloroform formed. The correlations of the concentration of chloroform, DOC and TOC were investigate in this study.

• 식물조직배양학회지
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• 제27권2호
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• pp.149-154
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• 2000

땅두릅 미성숙 화기절편을 이용하여 1 mg/L 2,4-D의 고체배지에서 배발생 캘러스를 유도하였고 동일조건의 액체배지에서 증식시켰다. 배발생 세포를 270$\mu\textrm{m}$의 걸름체로 거른 다음 배발생 세포는 싸이토카이닌이 포함된 MS 액체배지에서 2주 동안 배양하였고 그후 생장조절물질이 없는 MS 액체배지에서 5주간 배양하여 1차 체세포배가 유도되었다. 이들중 어뢰형과 자엽형 시기의 배를 생장조절물질이 없는 MS고체 배지에 치상하였을 때 캘러스의 형성 없이 2차배가 직접 유도되었다. 2차배를 유도할 때 2mg/L kinetin에서 얻어진 1차 배에서 가장 높은 빈도의 2차배가 발생되었다. 식물체 재생은 1 mg/L kinetin 혹은 1 mg/L zeatin을 처리하여 얻어진 1차 자엽형배로부터 유도된 2차 자엽형배를 생장조절물질이 없는 MS 고체배지에 치상하였을 때 높게 나타났다 (각각 25.4%, 28.6%). 2차배로부터의 3차배 발생과 식물체 재생과의 상관 관계를 볼 때 식물체의 재생에 있어서 3차배의 배발생은 저해적인 영향을 주었다.

• Archives of Pharmacal Research
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• 제16권3호
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• pp.244-250
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• 1993

Calli and suspension cultures were obtained following inoculation of the explant from leaves of Ginkgo biloba L on the supplemented MS basal medium. The obtained calli and suspension cultured cells were able to produce detectable amounts of ginkgolides which are known as natural specific PAF antagonists. The production of ginkgolides in the calli and suspension cultured celles were identified using GC/MS, GC and HPLC with authentic ocmpounds. Since the production of ginkgolides A and B the calli and suspension cultured cells had been confirmed, effects of types and concentration of plant growth regulators, media and illumination on the induction and growth of the callus were studied. The concentrations of growth regulators for optimal callus were studied. The concentrations of growth regulators for optimal callus induction were studied. The concentrations of growth regulators for optimal callus induction were 1.0 to 2.0 mg/L for NAA and o.1 mg/L for kinetin. The growth of the Callus seemed to be more simnultaed with the combination of NAA and kinetin than NAA and BA with illumination at all concentration ranges of 1.0 to 4.0 mg/l for NAA and o.1 to 1.0 mg/L for kinetin or BA studied. Amogn 8 different media used, the induction rate of callus on Anderson, Eriksson, and Shenk and Hildebrant at 4 weeks after the innoculation was almost the same as that of MS. However, callus was rarely induced on Heller or White medium. Suspension cultures were easily initiated with 3 g of callus (fresh weight) derived from ginkgo leaves on supplemented MS medium. A typical growth curve of suspension cultured cells could be obtained by measuring the fresh weight of the suspension cultured cells at every 3 days. To improve the growth of suspension cultured cells of ginkgo, effects of concentrations of NAA, sucrose, phosphate ions and molar ratio of $NH_{4}^+\;to\;NO_{3}^-$ ions in the culture medium were studied. The maximum growth of the cells was achieved when the culture medium contained 1.0 mg/L of NAA, 30 g/L sucrose, 1.75 mM phosphate ions and 1:5 molar ratio of $NH_{4}\;to\;NO_{3}^-$ ions.

• 식물조직배양학회지
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• 제25권1호
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• pp.7-11
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• 1998

Streptanthus 조직 배양 세포를 사용하여 사부를 순수분리 시키고, 분리된 사부에서 사부 하적에 대한 기작을 규명하기 위해 다음 연구를 하였다. 유조직 세포는 0.2% macerase 와 0.03% cellulase의 가수분해 효소로 처리하여 원형질체를 얻었고, 분화된 세포에서는 0.03% cellulase + 0.02% pectinase + 0.2% macerase + 0.025% rohamet PC로 가수분해시켜서 순수한 사부 세포와 사부 원형질체와 반세포의 원형질체를 분리하였다. 분리된 유조직 세포와 반세포의 원형질체에서는 단당류인 포도당을 수송시키나, 설탕은 수송시키지 못했다. 반면 분리된 사부 세포는 설탕을 능동수송 시키나 포도당은 수송시키지 못했다. 이는 설탕의 사부 하적은 반세포 없이도 가능하며, 반세포는 설탕 운반체가 없어서 설탕을 직접 수송할 수 있는 능력이 없다는 것을 보여주는 것이다. 그리고 사부 세포에서 설탕의 수송은 에너지대사에 의존하는 능동수송으로 나타났다.