• Plant Breeding and Biotechnology
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• 제5권4호
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• pp.269-281
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• 2017

With the continual development of genetically modified (GM) crops, it has become necessary to develop detailed and effective molecular characterization methods to select candidate events from a large pool of transformation events. Relative to traditional molecular analysis methods such as the polymerase chain reaction (PCR) and Southern blot hybridization, next generation sequencing (NGS) technology for whole-genome sequencing of complex crop genomes had proven comparatively useful for in-depth molecular characterization. In this study, four transformation events, including one in Bacillus thuringiensis (Bt)-resistant rice, one in resveratrol-producing rice, and two in beta-carotene-enhanced soybeans, were selected for molecular characterization. To merge NGS analysis and Southern blot-hybridization results, we confirmed the transgene insertion sites, insertion construction, and insertion numbers of these four transformation events. In addition, the read-coverage depth assessed by NGS analysis for inserted genes might provide consistent results in terms of inserted T-DNA numbers in case of complex insertion structures and highly duplicated donor genomes; however, PCR-based methods can produce incorrect conclusions. Our combined method provides an effective and complete analytical approach for whole-genome visual inspection of transformation events that require biosafety assessment.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• 제4권1호
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• pp.16-27
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• 2023

Primary succession on bare rocks is a challenge for invaders, including one of which was plants. The invasion of bare rock by exotic species raises the question of whether their presence hinders or facilitates succession. This study aimed to determine the role of exotic species in primary succession in six overburden (OB) coal mines using a chronosequence approach. Vegetation analysis was undertaken using line transects. Measurements were carried out on the absolute and relative coverage of each species. Native and exotic species were identified and grouped using information from local communities, identification books, and websites. The relationship between time and number of species, time, and relative dominance of exotic and native species was analyzed using Pearson's correlation. Species number and dominance data were analyzed descriptively. The number of native species from the six OB heaps was higher (57) than that from exotic heaps (50). Neither the number of species nor the coverage showed a significant relationship with time. Exotic species predominated throughout the age of the embankment but tended to decrease over time. Temporary dominance by exotic species plays a role in assisting primary succession in the OB. This process might be prolonged without the temporary dominance of exotic species during early primary succession.

• Molecules and Cells
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• 제26권3호
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• pp.250-257
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• 2008

Microsatellites or simple sequence repeats (SSR) are widely distributed in eukaryotic genomes and are informative genetic markers. Despite many advantages of SSR markers such as a high degree of allelic polymorphisms, co-dominant inheritance, multi-allelism, and genome-wide coverage in various plant species, they also have shortcomings such as low polymorphic rates between genetically close lines, especially in Capsicum annuum. We developed an alternative technique to SSR by normalizing and alternating anchored primers in random amplified microsatellite polymorphisms (RAMP). This technique, designated reverse random amplified microsatellite polymorphism (rRAMP), allows the detection of nucleotide variation in the 3' region flanking an SSR using normalized anchored and random primer combinations. The reproducibility and frequency of polymorphic loci in rRAMP was vigorously enhanced by translocation of the 5' anchor of repeat sequences to the 3' end position and selective use of moderate arbitrary primers. In our study, the PCR banding pattern of rRAMP was highly dependent on the frequency of repeat motifs and primer combinations with random primers. Linkage analysis showed that rRAMP markers were well scattered on an intra-specific pepper map. Based on these results, we suggest that this technique is useful for studying genetic diversity, molecular fingerprinting, and rapidly constructing molecular maps for diverse plant species.

• The Korean Journal of Ecology
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• 제26권2호
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• pp.71-74
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• 2003

Effects of nitrogen addition on the growth of Indigofera pseudo-tinctoria (Leguminosae) in the waste landfill site was investigated. Nitrogen fertilization in the nitrogen poor soils of waste landfill may influence the growth of nitrogen fixing plants beneficially or detrimentally. When I. pseudo-tinctoria was fertilized with three different levels of nitrogen, the coverage of plants treated with 46 g N/$m^2$ and 460 g N/$m^2$ was significantly less than that of plants treated with 23 g N/$m^2$. The growth rates of plant height treated with 46 g N/$m^2$ and 460 g N/$m^2$ were significantly less than those of plants treated with 23 g N/$m^2$. The growth rates of plant diameter treated with 46 g N/$m^2$ and 460 g N/$m^2$ were significantly less than those of plants treated with 23 g N/$m^2$. Dry weights of whole plants in control sites were higher than those of all the others nitrogen treatment sites. Nodule numbers were higher in control plants than those of plants in all the other nitrogen treatment sites. It is suggested that nitrogen fertilizer addition over 23 g N/$m^2$ affect the growth of some nitrogen fixing plants, such as I. pseudo-tinctoria, negatively.

• Molecules and Cells
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• 제27권2호
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• pp.205-209
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• 2009

The loci of the 5S and 45S rRNA genes were localized on chromosomes in five species of Capsicum, namely, annuum, chacoense, frutescens, baccatum, and chinense by FISH. The 5S rDNA was localized to the distal region of one chromosome in all species observed. The number of 45S rDNA loci varied among species; one in annuum, two in chacoense and frutescens, and chinense, and four in baccatum, with the exceptions that 'CM334' of annuum had three loci and 'tabasco' of frutescens gad one locus. 'CM334'-derived BAC clones, 384B09 and 365P05, were screened with 5S rDNA as a probe, and BACs 278M03 and 262A23 were screened with 25S rDNA as a probe. Both ends of these BAC clones were sequenced. FISH with these BAC probes on pachytenes from 'CM334' plant showed one 5S rDNA locus and three 45S rDNA loci, consistent with the patterns on the somatic chromosomes. The 5S rDNA probe was also applied on extended DNA fibers to reveal that its coverage measured as long as 0.439 Mb in the pepper genome. FISH techniques applied on somatic and meiotic chromosomes and fibers have been established for chili to provide valuable information about the copy number variation of 45S rDNA and the actual physical size of the 5S rDNA in chili.

• Nuclear Engineering and Technology
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• 제41권6호
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• pp.849-858
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• 2009

Risk caused by safety-critical instrumentation and control (I&C) systems considerably affects overall plant risk. As digitalization of safety-critical systems in nuclear power plants progresses, a risk model of a digitalized safety system is required and must be included in a plant safety model in order to assess this risk effect on the plant. Unique features of a digital system cause some challenges in risk modeling. This article aims at providing an overview of the issues related to the development of a static fault-tree-based risk model. We categorize the complicated issues of digital system probabilistic risk assessment (PRA) into four groups based on their characteristics: hardware module issues, software issues, system issues, and safety function issues. Quantification of the effect of these issues dominates the quality of a developed risk model. Recent research activities for addressing various issues, such as the modeling framework of a software-based system, the software failure probability and the fault coverage of a self monitoring mechanism, are discussed. Although these issues are interrelated and affect each other, the categorized and systematic approach suggested here will provide a proper insight for analyzing risk from a digital system.

• The Korean Journal of Ecology
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• 제22권5호
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• pp.277-285
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• 1999

도시의 식재림을 자연림으로 복원하기 위한 기초조사로서 서울시 강남구 청담동에 위치한 청담근린공원 내에서 교목층을 구성하고 있는 은수원사시나무를 벌목한 후 초기 2년간 식물상, 식생, 목본식 물의 생장량 변화를 조사하였다. 그 결과 다음과 같았다. 첫째, 벌목의 결과 목본식물종의 변화는 없었으나 초본식물은 1년 후 콩제비꽃 등 3종이 사라지고 15종이 새로 출현하여 종 수가 44% 증가하였다. 새로 침입한 종은 대부분 박주가리 등의 교란지종이거나 붉은서나물 등의 귀화종이었다. 둘째, 벌목은 기존의 아교목층이나 관목층을 구성하고 있던 갈참나무, 신갈나무, 상수리나무, 팥배나무 둥의 피도를 급격히 증가시켰다. 싸리의 피도는 주변의 다른 식물에 따라 변화가 심하였다. 셋째, 초본층의 구성종 중 맑은대쑥의 피도 증가가 가장 뚜렷하였으며 이외의 종의 것은 큰 변화가 없었다. 넷째, 참나무류는 피음이 제거되면 생장이 상당히 증가되었다. 실험구에서는 벌목 후 다음해의 DBH 증가는 갈참나무, 신갈나무 및 떡갈나무에서 각각 53.0%, 22.9% 및 28.8%, 대조구에서는 각각 23.1%, 8.3% 및 6.1%이었다. 그리고 가지의 생장량은 벌목 전해를 기준으로 벌목 다음해 실험구의 갈참나무, 신갈나무 및 떡갈나무에서 각각 565%, 191%, 644%이었고, 대조구의 것에서 각각 117%, 100%, 42%이었다. 그런데 갈참나무와 떡갈나무는 상대적으로 줄기보다 가지의 생장 증가가 뚜렷하였지만 신갈나무는 그 반대이었다.

• 환경생물
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• 제38권1호
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• pp.197-205
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• 2020

본 연구는 경관식재용 지피식물의 혼식 설계를 위하여 생활형이 다른 9종의 식물의 연중 생장 특성을 파악하고 식물들 간의 피복 특성을 분석하여 생활형에 따른 적정 식재조합을 도출하고자 본 연구를 실시하였다. 단립형으로 초장 20 cm 이상 생장하는 둥굴레, 맥문동, 일월비비추는 4월 식재 후 60일 이내 생육이 증가한 후 형태를 유지하였다가 9월 이후 지상부가 퇴화하며 휴면기에 들어가는 경향을 보였고, 분지형으로 20 cm 이하로 생장하는 바위취, 패랭이꽃, 애기기린초는 4월 식재 후 9월까지 지속적으로 생장하다가 9월 이후 생장이 감소하는 특성을 나타냈다. 포복형으로 지면에 붙어서 생장하는 리시마키아는 식재 후 3개월 경과부터 급속한 생장을 보였으며 돌나물은 8월까지 서서히 생장하다가 온도가 하강하는 9월부터 지상부가 퇴화하는 특성을 보였다. 꽃잔디는 초반 생장은 좋았으나 개화기가 지나고 난 후에는 전반적인 생육발달이 더뎌져 식재 후 7개월 동안 11.0%의 낮은 생장 특성을 보였다. 이와 같이 각기 다르게 생장하는 9종의 식물들을 혼식하였을 때 피복하는 특성을 조사한 결과 각 식물의 생장 특성이 피복에 영향을 미치는 것을 알 수 있었는데, 초장이 10~20 cm 자라며 로제트형으로 자라는 형태와 포복형 식물들의 조합이 높은 피복률을 나타냈다. 식물별로는 포복형인 돌나물이 맥문동, 일월비비추, 패랭이꽃, 애기기린초, 꽃잔디 등이 가장 많은 식물들과 혼식하였을 때 생장이 증가하는 종이었다. 로제트형 중에서는 일월비비추가 애기기린초, 바위취, 리시마키아, 돌나물과 동반생장이 가능한 종이었고, 지표면에서 분지가 밀생하여 자라는 패랭이꽃은 잎이 밀생하지 않는 맥문동, 바위취, 리시마키아, 돌나물과 혼식이 유리한 경향을 나타났다. 이 결과는 식물의 생장형 분류 중 근경이 분지하는 종보다 땅위로 뻗는 줄기로 번식하거나 무근경으로 영양 생장하는 종이 다른 종들과 혼식하기에 좋은 유형이라고 사료되었다.

• Journal of Ecology and Environment
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• 제33권4호
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• pp.377-389
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• 2010

Understanding the factors relevant to endangerment and the patterns of habitat locations in relation to protected areas is critically important for the conservation of rare species. Although 64 plant species have recently been listed as endangered species in Korea, this information has, until now, not been available, making appropriate management and conservation strategies impossible to devise. Thus, we collected information on potentially threatening factors, as well as information on the locations in which these species were observed. The potentially threatening factors were classified into seven categories. National parks, provincial parks, ecosystem conservation areas, and wetland conservation areas were defined as protected conservation areas. Korean digital elevation model data, along with the maps of all protected areas were combined with the maps of endangered plant species, and analyzed via Geographic Information Systems (GIS). Excluding the category of "small population", endangered plant species in Korea were associated more frequently with extrinsic factors than intrinsic factors. Considering land surface only, all conservation areas in Korea totaled 4.9% of the land, far lower than International Union for Conservation of Nature and Natural Resources (IUCN)'s 10% coverage target. At the species level, 69% of the endangered plant species were detected in conservation areas, mostly in national parks. However, this result demonstrates that 31% of endangered species inhabit areas outside the conservation zones. Furthermore, at the habitat level, a large proportion of endangered species were found to reside in unprotected areas, revealing "gaps" in protected land. In the face of rapid environmental changes such as population increases, urbanization, and climate changes, converting these gap areas to endangered species' habitats, or at least including them in habitat networks, will help to perpetuate the existence of endangered species.

• 한국습지학회지
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• 제25권3호
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• pp.184-195
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• 2023

한국에서 생태계교란 식물인 단풍잎돼지풀의 효과적인 관리방안을 마련하고자, 초식자인 돼지풀잎벌레의 기주특이성, 연간 이동거리를 조사하여 생물학적 방제 수단으로의 활용 가능성과 이 곤충의 섭식이 단풍잎돼지풀의 활력에 미치는 영향을 연구하였다. 이를 위해서 돼지풀잎벌레의 기주선호도를 조사하였고, 돼지풀잎벌레가 확인된 지점을 기준으로 거리가 10m, 20m, 30m, 100m 떨어진 지점에 돼지풀잎벌레가 없는 단풍잎돼지풀군락을 선정하여 돼지풀잎벌레의 출현 여부 파악을 통해 이들의 이동 거리를 확인하였다. 그리고 돼지풀잎벌레의 섭식에 따른 단풍잎돼지풀의 생육, 번식 그리고 생리반응을 측정하였다. 그 결과 돼지풀잎벌레는 국화과의 3분류군만을 섭식하였고, 숙주로부터 반경 30m/yr 이내로 단거리 이동하였다. 초식자의 섭식은 단풍잎돼지풀의 생육, 번식 그리고 생리반응에 부정적인 영향을 주었고, 초식자가 있는 곳으로부터 거리가 멀어질수록 생육 및 번식이 양호하였다. 또한, 단풍잎돼지풀의 식피율이 50% 이하일 때 초식자를 도입하면 90% 이상의 단풍잎돼지풀을 제어할 수 있었지만, 식피율이 90% 이상에서는 제거의 효과가 감소했다. 결과적으로 돼지풀잎벌레는 단풍잎돼지풀의 생태학적 제어자로서 활용 가능성이 있고, 제어의 효과를 높이기 위해서는 단풍잎돼지풀의 식피율이 낮은 봄(5월)에 이 곤충을 도입하는 것이 유리하다.