• Journal of Applied Biological Chemistry
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• v.66
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• pp.305-310
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• 2023

Bandiburichodang (BDC) is a new variety of Zea mays L. Total polyphenol content (TPC) assay and quantitative analysis of ferulic acid (FA) were performed to determine the steaming, roasting conditions of BDC kernels that lead to the highest content. TPC levels increased after roasting under all conditions. TPC levels in samples steamed at 115 ℃ for 25 min were 3.157 mg/g before roasted, and increased to 3.825 and 4.739 mg/g after roasting at 160 and 200 ℃, respectively. Whether BDC kernels were roasted was relevant with TPC content. BDC kernels were extracted to perform quantitative analysis of FA. Roasting temperature affected FA content: the higher the temperature, the lower the content. BDC kernels that were steamed at 115 ℃ for 25 min had 0.178 mg/g of FA content before roasting, and levels decreased to 0.132 and 0.115 mg/g after roasting. Under different roasting conditions, FA content decreased 15 to 50%. We hypothesize that this phenomenon is due to a breakdown of phenolic compounds or cell wall disruption.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.294-297
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• 2001

Sophorolipid, a biosurfactant produced from Candida bombicola ATCC 22214. showed antimicrobial activity against Bacillus subtilis. Staphylococcus xylosus, Streptococcus mutans, and Propionibacterium acne at 4, 1, 1, 0.5 ppm as MIC(minimum inhibitory concentration). Also 100ppm of sophorolipid inhibited 50% of cell growth of plant pathogenic fungus. Botrytis cinerea. However, sophorolipid showed no effect on the Escherichia coli, indicating its selective antimicrobial activity depending on the cell wall structure. Treatment of B. subtilis with sophorolipid increased the leakage of intracellular enzyme, malate dehydrogenase, indicating the possible interaction of sophorolipid with cellular membrane. Between lactone-type and acid-type sophorolipid, the former showed higher antimicrobial activity.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.27 no.2
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• pp.57-58
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• 2001

Biosurfactant has antibiological activities. Due to its low toxicity, biosurfactant can be applied to human health care products. Posseing the antibiological activities as well as the surfactant properties implies that biosurfactant can be widely used in the cosmetic industry. In this presentation, applications of biosurfactant have been reviewed including the antimicrobial activities of sophorolipid. Sophorolipid, a biosurfactant produced from Candida bombicola ATCC 22214, showed antimicrobial activity against Bacillus subtilis, Staphylococcus xylosus, Streptococcus mutans, and Propionibacterium acne at 4, 1, 1, 0.5 ppm as MIC (minimum inhibitory concentration). Also 100 ppm of sophorolipid inhibited 50% of cell growth of plant pathogenic fungus, B. cinera. However, sophorolipid showed no effect on the Escherichia coli., indicating its selective antimicrobial activity depending on the cell wall structure. Treatment of B. subtilis with sophorolipid increased the leakage of intracellular enzyme, malate dehydrogenase, indicating the possible interaction of sophorolipid with cellular membrane. Between lactone-type and acid-type sophorolipid, the former showed higher antimicrobial activity.

• Korean Journal of Plant Taxonomy
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• v.31 no.3
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• pp.183-222
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• 2001

Morphological characters of section Acutae of the genus Carex (Cyperaceae) were reexamined. The epidermal patterns of perigynium, achene and leaf were investigated using by a scanning electron microscope (SEM) and a light microscope (LM). Morphological characters such as length and width of stem. leaf, bract, spike, scale, perigynium and achene, and shape of cross-sectioned stem, spike, scale, apex of scale, perigynium, beak of perigynium and achene, and epidermal pattern of perigynium, achene and leaf (shape of fundamental epidermal cell and cell wall, type of silica body, shape of beak epidermal cell and cell wall in perigynium, subsidiary cell shape. size and frequency of stomatal complex of leaf were useful for the identification of the observed 11 taxa. C. cinerascens, C. heterolepis and C. forficula have been confused due to similar morphological characters. C. cinerascens, C. heterolepis and C. forficula were distinct. however with respect to from width of leaf, shpae of scale, apex of scale, perigynium, beak of perigynium, and epidermal patterns of achene and leaf.

• Korean Journal of Plant Taxonomy
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• v.33 no.3
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• pp.227-253
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• 2003

Morphological characters of C. kobomugi, C. gibba, C. echinata. C. remotiuscula. C. maakii. C. curta, and C. loliacea of genus Carex were rexamined. The epidermal patterns of perigynium, achene and leaf were investigated using by a scanning electron microscope(SEM) and a light microscope(LM). Morphological characters. such as length and width of stem and leaf, sheath, bract, spike, scale, perigynium, beak of perigynium, length of spike peduncle, size, and frequency of stomatal complex of leaf, number of bract, shape of stem transection, scale and apex of scale, beak and base of perigynium, achene, epidermal cell and cell wall of perigynium, achene, leaf epidermal patterns (fundamental epidermal cell and cell wall, silica body, subsidiary cell), hair, papillae, prickle present/absent of perigynium and leaf were useful for the identification of observed seven taxa. Examined six taxa of section Macrocephalae, Gibbae, Stellulatae, Elongatae, Ovales, and Heleonates were distint from each other with respect of length and width stem, leaf, bract, perigynium, perigynium beak, lenght of spike peduncle, perigynium, and leaf. A key based on data was presented here.

• Korean Journal of Plant Taxonomy
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• v.32 no.3
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• pp.257-292
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• 2002

Morphological characters of sections Foetidae(one taxon), Arenariae(one taxon) and Multiflorae(six taxa) of genus Carex(Cyperaceae) were reexamined. The epidermal patterns of perigynium, achene and leaf were investigated using by a scanning electron microscope(SEM) and a light microscope(LM). Morphological characters, such as length and width of stem, leaf, leaf sheath, bract, spike, scale, perigynium and beak of perigynium, length of spike peduncle, size and frequency of stomatal complex of leaf, number of bract, shape of stem transection, scale and apex of scale, beak and base of perigynuim, achene, epidermal cell and cell wall of perigynium, achene, leaf epidermal patterns(fundamental epidermal cell and cell wall, silica body, subsidiary cell), hair, papillae present/absent of perigynium, and leaf were useful for the identification of observed eight taxa. According to the current study, examined eight taxa of sections Foetidae, Arenariae and Multiflorae were distinct from each other with respect to length and width of stem, leaf, bract, perigynium, perigynium beak, length of spike peduncle, shape of bract, scale and apex of scale, perigynium, perigynium beak, hair presence/absence of perigynium and leaf. A key based on data was presented here.

• Horticultural Science & Technology
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• v.34 no.5
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• pp.692-700
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• 2016

The objective of this study was to determine the differences in fruit growth, fruit quality, and particularly the pedicel vascular bundles of 'Shigyoku' and 'Heukboseok' grapes, which appeared to be different in softening at harvest. 'Shigyoku' grape matured faster (by about 20 days) than 'Heukboseok' grape with slight fruit enlargement after veraison. However, fruit of 'Heukboseok' grapes showed remarkable enlargement in both the primary and secondary fruit enlargement periods. Hypodermal cell layers were not different after veraison in 'Shigyoku' grape, but degradation of the hypodermis cell wall continued in 'Heukboseok' grape, resulting in a gradual decline in firmness. The numbers of hypodermal cell layers in 'Shigyoku' and 'Heukboseok' grapes were 14.2 and 9.0, respectively. The average content of soluble solids in 'Shigyoku' grape ($19.5^{\circ}Brix$) was significantly ($p{\leq}0.01$) higher than that of 'Heukboseok' grape ($17.0^{\circ}Brix$). Xylem of the pedicel did not differ between the two varieties. However, average phloem area after veraison of 'Shigyoku' grape ($19044.8{\mu}m^2$) was about 1.8 fold greater than that of 'Heukboseok' grape ($10509.4{\mu}m^2$), based on the number of cells constituting the phloem. The cell number and area of the phloem might affect the accumulation of sugars, the main constituents of the cell wall, thus maintaining the firmness of grapes until late maturity. Therefore, the increased softening of 'Heukboseok' grapes at harvest might due to their phloem structure.

• The Plant Pathology Journal
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• v.28 no.4
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• pp.401-408
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• 2012

Initial subcellular responses in susceptible (PI 274420) and resistant (cv. Hartwig) soybeans infected with the soybean cyst nematode (SCN) were examined 2 and 4 days after inoculation (DAI). Subcellular features common to both soybeans at 2 DAI included hypertrophied initial syncytial cells (ISCs) and syncytium-component cells (SCs) with a dense cytoplasm containing proliferated rough and smooth endoplasmic reticulum (RER and SER), a hypertrophied nucleolus, and reduced vacuoles, suggesting that the nematode-infected cells were dedifferentiated. In the resistant soybean, a striking initial subcellular difference from the susceptible soybean was the dilation of the RER, indicating ER dysfunction and leading to cell death. This disturbed nematode feeding, as evidenced by disrupted feeding tubes. In PI 274420, the ISC cytoplasm was depleted, with the exception of ER membranes, at 4 DAI, while the SC cytoplasm was dense with proliferation of starch-containing plastids around multiple nuclei that might be derived from the congregation of nuclei in the neighboring SCs and in part by nuclear division without cytokinesis. In cv. Hartwig, syncytia were necrotized with secondary cell wall thickening outside the plasma membrane and an extremely dense cytoplasm containing a nucleus with an electron-lucent nucleolus, accompanied by the proliferation of closely stacked parallel RER and ribosomes. These results suggest that syncytia develop continuously in PI 274420 to produce and store nutritional substances in SCs, providing for the nematode through ISC until maturation, but in cv. Hartwig, syncytia degenerate early due to excessive metabolism, blocking nematode feeding and cytoplasmic connections with adjacent intact cells.

• The Plant Pathology Journal
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• v.37 no.2
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• pp.173-181
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• 2021

The genus Streptomyces demonstrates enormous promise in promoting plant growth and protecting plants against various pathogens. Single and consortium treatments of two selected Streptomyces strains (Streptomyces shenzhenensis TKSC3 and Streptomyces sp. SS8) were evaluated for their growth-promoting potential on rice, and biocontrol efficiency through induced systemic resistance (ISR) mediation against Xanthomonas oryzae pv. oryzicola (Xoc), the causal agent of rice bacterial leaf streak (BLS) disease. Seed bacterization by Streptomyces strains improved seed germination and vigor, relative to the untreated seed. Under greenhouse conditions, seed bacterization with consortium treatment TKSC3 + SS8 increased seed germination, root length, and dry weight by 20%, 23%, and 33%, respectively. Single and consortium Streptomyces treatments also successfully suppressed Xoc infection. The result was consistent with defense-related enzyme quantification wherein single and consortium Streptomyces treatments increased peroxidase (POX), polyphenol oxidase, phenylalanine ammonia-lyase, and β,1-3 glucanase (GLU) accumulation compared to untreated plant. Within all Streptomyces treatments, consortium treatment TKSC3 + SS8 showed the highest disease suppression efficiency (81.02%) and the lowest area under the disease progress curve value (95.79), making it the best to control BLS disease. Consortium treatment TKSC3 + SS8 induced the highest POX and GLU enzyme activities at 114.32 µmol/min/mg protein and 260.32 abs/min/mg protein, respectively, with both enzymes responsible for plant cell wall reinforcement and resistant interaction. Our results revealed that in addition to promoting plant growth, these Streptomyces strains also mediated ISR in rice plants, thereby, ensuring protection from BLS disease.

• Journal of Plant Biology
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• v.35 no.1
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• pp.45-51
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• 1992

The changes of protein bodies in endosperm cells of both seeds with red seed coat and indehiscent seeds of Panax ginseng C.A. Meyer have been investigated in relation to the embryo development. In the early stage of seeds with red seed coat, spherical spherosomes were distributed in endosperm cells. Protein bodies were formed from vacuoles containing the storage protein. Cell organelles were hardly observed in the cytoplasm. In the late stage of the seed with red seed coat, the endosperm was filled with spherosomes and protein bodies. The protein bodies consisted of amorphous inclusions with high electron density or proteinaceous matrix with even electron density. In the seed of in dehiscence, the protein body in endosperm cells contained globoids and protein crystalloids. The globoid of protein body had a electron dense materials. Umbiliform layer was formed between embryo and endosperm. The deformation patterns of endosperm cell wall and the cellulose microfibril were observed in endosperm cells near the umbiliform layer. Umbiliform layer consisted of lipid body and autolyzed cell debris. The protein body of endosperm cell near the umbiliform layer showed various degenerative patterns, and so electron density of proteinaceous matrix was gradually decreased.reased.