• 한국초지조사료학회지
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• 제20권1호
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• pp.7-12
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• 2000

오차드그래스의 종자배양에서 형성된 캘러스를 현탁배양하여 배양기간별 부정배 형성정도와 식물체 재분화율 등에 대한 몇 가지 실험을 수행하여 얻은 결과를 요약하면 다음과 같다. 종자유래의 캘러스를 현탁배양하였을 때, 세포 모양이 둥근것과 그들의 세포괴는 배양 50일 후에 최대치를 나타내었고 그 이후는 감소하였다. 현탁배양 기간에 따른 갤러스의 부정배 형성과 식물체 분화율은 배양 60일째 가장 높았으며, 그 이후는 감소하는 경향이었다. 현탁배양에서 배지 내에 첨가되는 casein hydrolysate (CH)의 적정농도는 $4\;g/{\ell}$인 것으로 나타났다.

• Journal of Plant Biotechnology
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• 제7권2호
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• pp.129-134
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• 2005

Callus induction from a leaf explant has been achieved in Lilium Oriental hybrid 'Casa Blanca'. The highest frequency of callus induction was obtained on MS medium supplemented with 0.5 mg/L BA and 2.0 mg/L NAA after 2 months of culture. The cultures maintained continuously without change in color and type of callus when they cultured in the dark. Plantlet regeneration with a high frequency was achieved from induced calli on the same medium. A number of shoots are formed from one cluster of callus, and bulblets developed into intact plantlets after transfer to hormone-free MS medium. No phenotypic variations were observed among regenerants. Enhancement in plantlet regeneration via callus formation would be expected to facilitate the efficiency of transformation of this Oriental hybrid 'Casa Blanca'.

• Journal of Plant Biotechnology
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• 제33권2호
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• pp.105-110
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• 2006

배지 내 식물생장조절물질, 무기물 농도, 질소원 비율이 G. sylvestre 세포배양에 미치는 영향을 알아보고자 실험을 수행하였다 1.0 mg L$^{-1}$ 이상2,4-D 처리시 세포 생장량이 급격히 감소한 반면, 0.5 mg L$^{-1}$ 이하 kinetin 처리구들은 세포 생장량이 큰 차이가 없었다. 따라서 G. sylvestre 세포배양의 경우 2,4-D 1.0 mg L$^{-1}$와 kinetin 0.1 mg L$^{-1}$를 함께 사용하는 것이 세포 생장량 증대에 효율적이라고 판단되었다. 무기물 농도가 G. sylvestre 세포 생장량에 미치는 영향을 조사한 결과 1x MS에서 세포 생장량이 가장 높았으며, 배지 내 무기물 농도가 1x MS 이상일 경우 배지의 낮은 수분포텐셜로 인해 세포생장이 크게 억제되었다. $NH_4^+:NO_3^-$ 비율이 0:60에서 가장높은 생장량을 나타내었으나 세포의 크기가 작고 백색을 나타내었으며 연속배양시 생장량이 점차 감소하므로, G. sylvestre 세포를 연속 배양할 경우 $NH_4^+:NO_3^-$를 20:40으로 사용하는 것이 효과적이라고 생각되었다.

• 한국식물학회:학술대회논문집
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• 한국식물학회 1995년도 식물학심포지움 식물로부터 유용 2차대사산물의 생산 PRODUCTION OF USEFUL SECONDARY METABOLITES FROM PLANTS
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• pp.57-66
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• 1995

• Journal of Plant Biology
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• 제32권4호
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• pp.313-322
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• 1989

A system was established for induction of multi-shoots and plant regeneration from mesophyll protoplasts of alfalfa, Medicago sativa L. cv. Vernal. Different hormonal effects were tested at each step of protoplast culture, i.e. cell division in modified Kao's liquid medium (K566-7). calli formation on SH semi solid medium, and multi-shoot regeneration from calli on SHa and SHb solid media. Frequency of multi-shoots and plant regeneration was affected by various combinations of phytohormones in final step. The evaluation of multi-shoots induction systems via protoplast culture was discused.

• Plant Biotechnology Reports
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• 제5권4호
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• pp.367-373
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• 2011

Linum album accumulates anti-tumor podophyllotoxin (PTOX) and its related lignans, which were originally isolated from an endangered species Podophyllum. In the present study, we examined the effects of five fungal extracts on the production of lignans in L. album cell cultures. Fusarium graminearum extract induced the highest increase of PTOX [$143{\mu}g\;g^{-1}$ dry weight (DW) of the L. album cell culture], while Rhizopus stolonifer extract enhanced the accumulation of lariciresinol up to $364{\mu}g\;g^{-1}$ DW, instead of PTOX. Typical elicitors, such as chitin, chitosan, or methyl jasmonate (MeJA), were shown to be less effective in lignan production in L. album cell cultures. These results verified the advantages of fungal extracts to increase lignan production in L. album cell culture, and suggested potential on-demand metabolic engineering of lignan biosynthesis using differential fungal extracts.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 1996년도 International Symposium on the Development of Anti Cancer Resources From Plants및 1996년도 한국자원식물학회 정기학술 발표회
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• pp.31-33
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• 1996

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권3호
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• pp.138-149
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• 2002

Plant cell culture provides a viable alternative over whole plant cultivation for the production of secondary metabolites. In order to successfully cultivate the plant cells at large scale, several engineering parameters such as, cell aggregation, mixing, aeration, and shear sensitivity are taken into account for selection of a suitable bioreactor. The media ingredients, their concentrations and the environmental factors are optimized for maximal synthesis of a desired metabolite. Increased productivity in a bioreactor can be achieved by selection of a proper cultivation strategy (batch, fed-batch, two-stage etc.), feeding of metabolic precursors and extraction of intracellular metabolites. Proper understanding and rigorous analysis of these parameters would pave the way towards the successful commercialization of plant cell bioprocesses.

• KSBB Journal
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• 제30권2호
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• pp.82-90
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• 2015

Plant cell immobilization can protect plant cells from shear forces and increase the stability of gene. An additional advantage of immobilization is the easiness for performing continuous culture with cell recycling. Therefore plant cell immobilization can overcome the limitations of plant cell applications. In addition, target protein should be selected from pharmaceutical proteins to get rid of low expression level problem. The enhanced production of human granulocyte-macrophage colony-stimulating factor (hGM-CSF) was investigated in immobilized Nicotiana tabacum suspension cell cultures. When the cells were immobilized in polyurethane foam, specific production of hGM-CSF was higher than that in alginate bead immobilization. Optimum continuous culture condition was the addition of 60 g/L sucrose in growth media with exchanging media every 6 day. Under the same condition, specific hGM-CSF production was 7 times higher in a 500-mL spinner flask than that in 100-mL Erlenmeyer flasks. Therefore, development of an effective immobilization process would be possible when the advantage of easy cell recycling was used. Consequently, enhanced production of target proteins could be possible in immobilized continuous cultures when the advantages of immobilization were applied.

• 한국미생물·생명공학회지
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• 제17권4호
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• pp.343-348
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• 1989

L. erythrorhizon 세포를 polyurethane foam과 함께 증식시킬 경우 shikonin 유도체가 polyurethane에 효과적으로 흡착됨과 동시에 polyurethane을 사용하지 않은 경우와 비교하여 shikonin 생산량이 현저히 증가하였다. 이 같은 증가는 세포를 polyurethane pore에 고정하여 증식시킴으로써 원활한 세포간 접촉을 유지하고 세포 내에 shikonin 농도를 저하시켜shikonin 생성에 좋은 조건을 제공함에 기인한 것으로 생각되었다. 공정의 생산성을 높이기 위하여 여러가지 배양시스템이 검토되었는데, indole-3-acetic acid(1.75mg/ι)와 kinetin(0.1mg/ι)을 함유하는 Schenk-Hildebrandt 배지 (SHIK 배지) 시스템이 가장 효과적이었다. p-Chlorophenoxyacetic acid (2.0 mg/ι)와 kinetin (0.1 mg/ι)를 함유하는 Schenk-Hildebrandt 배지 (SHND 배지) 시스템에 비교하여 SHIK 배지 시스템에서 Shikonin 생성량은 약 4.5배 증가하였다. Polyurethane을 세포를 고정화하는 지지체로 사용할 경우에는 현재 행하여지고 있는 2단계 배양보다 1단계 배양이 더욱 효과적이며 경제적으로도 매우 유리할 것으로 판단되었다.