• 제목/요약/키워드: plant care

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Development of transgenic disease-resistant root stock for the growth of watermelon

  • Cho, Song-Mi;Chung, Soo-Jin;Moon, Sun-Jin;Kim, Kwang-Sang;Kim, Young-Cheol;Cho, Baik-Ho
    • 한국식물생명공학회:학술대회논문집
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    • 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
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    • pp.62-65
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    • 2004
  • To protect the watermelon against soil-borne pathogens, we are currently producing disease-resistant transgenic root stock for the growth of watermelon, A defensin gene (J1-1) from Capsicum annum, a ACC deaminase gene from Pseudomonas syringae, a galactinol synthase (CsGolS) gene from Cucumis sativus, and a WRKY (CvWRKY2) gene from Citullus vulgaris were used as transgenes for disease resistance. The gene were transformed into a inbred line (6-2-2) of watermelon, Kong-dae watermelon and a inbred line (GO702S) of gourd, respectively, by Agrobacterium-mediated transformation. Putative transgenic plants were selected in medium containing 100mg/L kanamycin, and then integration of the genes into the genomic DNA were demonstrated by PCR analysis. Successful integration of the gene in regenerated plants was also confirmed by PCR (Figf 1), genomic Southern blot (Fig 2), RT-PCR (Fig 3), and Northern blot analysis(Fig 4). Several T1 lines having different transgene were produced, and disease resistance of the T1 lines are under estimation.

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근로자들의 교대근무 경험 (Workers' Experiences in Shift Work)

  • 김영혜;김영미;구미지;김소희;이내영;장경오
    • 지역사회간호학회지
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    • 제18권2호
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    • pp.284-292
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    • 2007
  • Purpose: The purpose of this study is to explore and understanding the nature of the shift workers' experience in industries requiring 24 hours-a-day service provision and support. For the Purpose, the present researcher made a research Question, "What are workers' experience in the shift work?" Methods: In the study, 5 male shift workers, 3 female shift worker and a woman (key informant) whose husband is a shift worker participated. Then profound interviews with the participants were made after their agreement. Results: The researcher classified the significant statements under 6 theme clusters, 1) sense of difference; 2) worries about health; 3) draining of emotion; 4) anxiety; 5) sadness; and 6) being comfortable. Conclusions: Shift work which is inevitable in modern society may have severe influences on shift workers' physical, mental and psychological aspects by causing their work cycle rhythm to be unbalanced. Finally, the researcher hopes that the results of the study would help understand workers' life and increase social concern and support to the workers.

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나무의사 제도 법제화에 따른 식물병리학회의 역할 (Legalization of Tree Doctor System and the Role of KSPP)

  • 차병진
    • 식물병연구
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    • 제23권3호
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    • pp.207-211
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    • 2017
  • 2016년 12월에 산림보호법 중 일부가 개정되어 이른바 '나무의사법'이 공포되었으며, 이 법은 2018년 6월 28일부터 시행될 예정이다. 새로운 법안에서는 나무의사 자격 소지자에 한하여 나무병원을 개업할 수 있고, 나무병원만이 생활권 공공분야의 수목병해충 관리를 할 수 있도록 규정하고 있으며, 그에 따라 '나무의사'라는 국가공인자격이 신설되었다. 나무의사가 되기 위해서는 지정된 양성과정을 이수하고 시험을 통과하여야 한다. 현재 산림청에서는 이 법의 시행을 위하여 구체적 시행방안을 포함하는 시행령과 시행규칙 등을 만들고 있다. 수목진료 및 건강관리에서 가장 핵심적인 부분이 식물병리학이라는 사실을 감안할 때, 그리고 식물병리학을 공부하는 학생들의 사회진출을 위하여 식물병리학회는 새로운 수목진료체계가 정착될 수 있도록 나무의사 양성기관의 교육과정 개발 및 나무의사 선발시험계획 수립 등에 적극적으로 관여하여야 할 것으로 생각한다.

Development of transgenic disease-resistance root stock for growth of watermelon.(oral)

  • S.M. Cho;Kim, J.Y.;J.E. Jung;S.J. Mun;S.J. Jung;Kim, K.S.;Kim, Y.C.;B.H. Cho
    • 한국식물병리학회:학술대회논문집
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    • 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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    • pp.65.2-65
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    • 2003
  • To protect the plant against several soil-borne pathogens, we are currently constructing disease-resistant transgenic root stock for the growth of cucurbitaceae vegetable plants, watermelon and gourd. We made a watermelon cDNA library from Cladosporium cucumerinum-Infected leaves for substractive hybriazation and differential screening. We isolated the several pathogen inducible cDNA clones, such as caffeoyl-CoA-methyltransferase, LAA induced protein, receptor-like kinase homolog, hydroxyproline-rich glycoprotein, catalase, calmodulin binding protein, mitochondrial ATPase beta subunit, methyl tRNA synthetase and WRKY transcription factors. We previously obtained CaMADS in pepper and galactinol synthase ( CsGolS) in cucumber that were confirmed to be related with disease-resistance. CaMADS and CsGolS2 were transformed into the inbred line 'GO701-2' gourd, the inbred line '6-2-2' watermelon and the Kong-dye watermelon by Agrobacterium tumerfaciens LBA4404. Plant growth regulators (zeatin, BAP and IAA) were used for shoot regeneration and root induction for optimal condition. Putative transgenic plants were selected in medium containing 100mg/L kanamycin and integration of the CaMADS and CsGO/S2 into the genomic DNA were demonstrated by the PCR analysis. We isolated major soil-borne pathogens, such as Monosporascus cannonballus, Didymella bryoniae, Cladosporium cuvumerinum from the cultivation area of watermelon or root stock, and successfully established artificial inoculation method for each pathogen. This work was supported by a grant from BioGreen 21 program, Rural Development Administration, Republic of Korea.

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The Use of Plants in Indigenous Health Care Practice of the Hajong Tribe Community in North Eastern Bangladesh

  • Rana, Md. Parvez;Sohel, Md. Shawkat Islam;Akhter, Sayma;Hassan, Mohammad Rakibul
    • Journal of Forest and Environmental Science
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    • 제25권1호
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    • pp.25-33
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    • 2009
  • An ethno-medical investigation was carried out to understand the use of plants in indigenous health care practice of the Hajong tribe community in North Eastern Bangladesh. The study of ethnobotany relating to any tribe is in itself a very intricate or long-winded process. The present study was done through structured questionnaires in consultations with the tribal practitioners and has resulted in the documentation of 25 plant species belonging to 21 families including herbs (36%), shrubs (32%), trees (28%) and climber (4%) were frequently used by the Hajong tribe for curing 27 ailments. For curing ailments, the use of aboveground plant parts was higher (86.67%) than the underground plant parts (13.33%). Leaf was used in the majority of cases for medicinal preparation (13 species), followed by fruits (7 species), root/rhizome (4 species), whole plant (3 species) and besides these, seed, latex and bark were used one species each, respectively. The study thus underlines the potentials of the ethnobotanical research and the need for the documentation of indigenous healthcare knowledge pertaining to the medicinal plant utilization for the greater benefit of mankind.

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The anti-hyperglycemic property of different ginseng partitions

  • Xie, Jing-Tian;Wang, Chong-Zhi;Kim, Stephen;Yuan, Chun-Su
    • Advances in Traditional Medicine
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    • 제5권1호
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    • pp.1-15
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    • 2005
  • Ginseng is a popular medicinal plant highly valued throughout the world. Asian ginseng is one of the most common species of ginseng. It has long occupied a significant position in oriental medicine and has been justified its name as the 'king herb'. As a nutritional supplement, ginseng is an extremely common and popular herbal medicine in the United States and Canada in recent decades. The multiple constituents of ginseng possess equally multifaceted pharmacological actions as demonstrated by numerous studies. Ginseng root and its constituents influenced the central nervous system, endocrine, cardiovascular, gastrointestinal system, sexual, renal organ and immune system, etc. One important action is its anti-hyperglycemic effect. Previous studies on ginseng demonstrate that only the root of ginseng has been used in the treatment of diabetes, while the other parts of ginseng plant were always neglected. Recently, we analyzed the constituents of ginseng berry, leaf and discovered that ginseng berry, leaf extracts and its total ginsenosides have the ability to reduce hyperglycemia and body weight and increase the peripheral glucose utilization in obese or diabetic ob/ob or db/db mice. Our data suggest that all parts of ginseng plant, including root, berry, leaf and stem exhibit potent anti-hyperglycemic and anti-obese effects and may provide an opportunity to develop a novel class of anti-diabetic agents.