• Title/Summary/Keyword: plant

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A Novel Protein Elicitor PeBL2, from Brevibacillus laterosporus A60, Induces Systemic Resistance against Botrytis cinerea in Tobacco Plant

  • Jatoi, Ghulam Hussain;Lihua, Guo;Xiufen, Yang;Gadhi, Muswar Ali;Keerio, Azhar Uddin;Abdulle, Yusuf Ali;Qiu, Dewen
    • The Plant Pathology Journal
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    • v.35 no.3
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    • pp.208-218
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    • 2019

  • Here, we reported a novel secreted protein elicitor PeBL2 from Brevibacillus laterosporus A60, which can induce hypersensitive response in tobacco (Nicotiana benthamiana). The ion-exchange chromatography, high-performance liquid chromatography (HPLC) and mass spectrometry were performed for identification of protein elicitor. The 471 bp PeBL2 gene produces a 17.22 kDa protein with 156 amino acids containing an 84-residue signal peptide. Consistent with endogenous protein, the recombinant protein expressed in Escherichia coli induced the typical hypersensitive response (HR) and necrosis in tobacco leaves. Additionally, PeBL2 also triggered early defensive response of generation of reactive oxygen species ($H_2O_2$ and $O_2{^-}$) and systemic resistance against of B. cinerea. Our findings shed new light on a novel strategy for biocontrol using B. laterosporus A60.

  • https://doi.org/10.5423/PPJ.OA.11.2018.0276 인용 PDF KSCI HTML

Recovery of Plant Cell and Its Debris by pH Control (pH 조절에 의한 식물세포 및 세포조각 회수)

  • 김진현
    • KSBB Journal
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    • v.15 no.4
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    • pp.405-407
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    • 2000

  • A novel recovery method was developed to obtain the plant cell and its debris from Taxus chinensis cell cultures By pH control of plant cell cultures plant cell and debris was precipitated. The best pH is between 1.8 and 2.2 to obtain the precipitate of the plant cell and debris. Also paclitaxel is stable in this acidic conditions. This method is very simple an efficient to recover the plant cell and debris from plant cell cultures.

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