• Title/Summary/Keyword: pine species

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Inhibitory Effects of Pine Cone (Pinus densiflora) on Melanogenesis in B16F10 Melanoma Cells

  • Lee, Seung-Hyun;Jang, Tae-Won;Choi, Ji-Soo;Mun, Jeong-Yun;Park, Jae-Ho
    • Korean Journal of Plant Resources
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    • v.32 no.4
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    • pp.275-281
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    • 2019
  • The pathological condition of excessive melanogenesis causing freckles, melasma, senile lentigo, pigmented acne scars, and cancer has a critical impact on the wellness of individuals. The mechanism of melanogenesis is related to the expression of melanogenic enzymes. Here, we evaluated the inhibitory effect of pine cone (Pinus densiflora) extracts on melanogenesis. P. densiflora, the Korean Red Pine, is the predominant tree species in the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. P. densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones are rarely used as natural raw materials, although they contain many bioactive phytochemicals. The pine cone ethyl acetate fraction (PEF) showed no toxicity to B16F10 cells at a concentration of less than $100{\mu}g/mL$. PEF inhibited the expression of microphthalmiaassociated transcription factor (MITF), tyrosinase and tyrosinase-related factors in B16F10 cells treated with 3-Isobutyl1-methylxanthine (IBMX). These results suggest that pine cones can be used as an effective natural melanogenesis inhibitory agent.

Anti-inflammatory Activity of Cone from Red Pine (Pinus densiflora)

  • Choi, Ji-Soo;Sung, Ji-Ho;Jang, Tae-Won;Mun, Jeong-Yun;Im, Jong-Yun;Park, Jae-Ho
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.119-119
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    • 2019
  • Pinus densiflora, the Korean Red Pine, is the predominant tree species of the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. Pinus densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones contain many bioactive phytochemicals, but they are rarely used as natural raw materials. This study was conducted to evaluated the anti-inflammatory effect of pine cone extracts and its possbility of natural sources were evaluated. Pine cones were extracted with 80% methanol, concentrated and then partitioned with ethyl acetate, and the organic layer was used as a sample. The Pine cone Ethyl acetate Fraction (PEF) showed no toxicity to RAW 264.7 cells at a concentration of less than $50{\mu}g/ml$. PEF inhibited the production of nitric oxide (NO) in RAW 264.7 cells treated with lipopolysaccharide (LPS). Also, It suppressed the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and transcription of nuclear factor-kappa B (NF-${\kappa}B$). These results suggest that pine cones can be used as an effective natural material for anti-inflammatory agent.

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Ectomycorrhizal Mushroom Occurrence around the Fairy Ring of Tricholoma matsutake at a Pine-Mushroom Forest (송이 발생림의 송이 균환 주변에 출현하는 외생균근성 균류)

  • Park, Hyun;Ka, Kang-Hyeon;Ryoo, Cheon-In;Kim, Kyo-Soo;Kim, Hyun-Joong
    • The Korean Journal of Mycology
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    • v.26 no.3 s.86
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    • pp.306-313
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    • 1998
  • The occurrences of ectomycorrhizal fungi were investigated in a pine-mushroom forest at Hongcheon, Korea. The fairy rings of Tricholoma matsutake were figured by the sporocarp places of T. matsutake for three years $(1995{\sim}1997)$, and the occurrences of other ectomycorrhizal fungi were surveyed with x and y dimensions for two years $(1996{\sim}1997)$. The diameters of fairy rings of T. matsutake ranged from 2m to 10m, which indicated that the age of the fairy rings as $10{\sim}50$ years when we consider that the growth of the fairy ring used to show about 10 cm per year. Russula bella, R. sororia, R. delica and Cantharellus minor were the major species occurred on the site during the survey period, and each species occupied 16.0%, 12.8%, 12.4% and 7.0% of total mushroom occurrence, respectively. From the results, we could conclude that the surveyed stand was a productivity-declining forest from the view point of pine-mushroom production. In addition, Amanita pantherina, Suillus bovinus, Ramaria flaccida and Laccaria amethystea were considered to be the indicator species for declining of pine-mushroom productivity since some fruiting bodies of the species appeared around the fairy ring of T. matsutake.

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Ground Beetle Fauna in Pinus densiflora Forests in Yangyang-gun, Kangwon Province, With a Special Reference to the Outbreaks of the Pine Needle Gall-Midge (Thecodiplosis japonensis) (강원도(江原道) 배양군(裴陽郡) 소나무림 내의 딱정벌레 상(相) - 솔잎혹파리의 피해(被害)와 관련(關連)하여 -)

  • Kubota, Kohei;Kim, Jong Kuk;Lee, Chan Young;Furuta, Kimito
    • Journal of Korean Society of Forest Science
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    • v.90 no.5
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    • pp.632-642
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    • 2001
  • Fauna, especially Carabidae (Coleoptera), on the floor of Pinus densiflora forests was investigated at five plantations and was compared with that in a mixed natural forest of pine and deciduous trees. There was little difference in the ground beetle fauna among the five P. densiflora plantations, and the bio-diversity of soil layer fauna was poor in all the pine plantations. Individual numbers of the beetles belonging to the genus Synuchus with increasing the infestation rate of the pine needle gall-midge (Thecodiplosis japonensis). These beetles seem to be important predators of the gall-midge. Comparing to the mixed forest, the number of higher taxa and the numbers of genera and species of Carabidae were all small in the pine forests. And especially, most flightless species were found only in the mixed forest. Thus, it is concluded that many species of Carabidae which had been lost from the lower mountainous areas of Korea have not recovered yet in the pine forests.

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Analysis of Species and Manufacturing Techniques for Wood Coffins of Bae's Family, Excavated in Youngdong, Korea (영동지역 배씨 문중 목관의 수종 및 가공법 분석)

  • Kim, Mun-Sung;Park, Won-Kyu
    • Journal of the Korea Furniture Society
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    • v.20 no.1
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    • pp.95-104
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    • 2009
  • The study was carried out to analyze wood quality of coffin board, the wood species of coffin excavated in Youngdong, Korea. All coffin woods were identified as hard pines, Korean red pine(Pinus densiflora S. & Z.). According to wood quality of coffins, inner coffins of Bae Cheon-Jo and his wife showed similarity and dissimilarity comparing with several literatures. The similarity was in the structure of panels, bottom lining board and charcoal layer, and the dissimilarity was in jointing. From the analysis of fibrous materials attached to coffin woods, the paper mulberry and cotton fibers were identified. In the inner coffin of Bae Cheon-Jo, tool traces by handle planer were observed.

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A New Species of Inostemma (Hymenoptera: Platygasteridae), A Larval Parasite of The Pine Gall Midge, Thecodiplosis sp. (Diptera; Cecidomyiidae) (솔잎혹파리 기생봉의 1신종)

  • KO JE HO
    • Korean journal of applied entomology
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    • v.19 no.1 s.42
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    • pp.35-38
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    • 1980
  • A new species of Platygasteridae, Inostemma hockpari (n. sp.) is described from Korea. This new species is an important biological control agent of the pine gall midge together with Inostemma seoulis, Platygaster matsutama and Inostemma matsutama (Collected in Jinhae by the author) in Korea.

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Species Identification of Wooden Structural Members of the Beomeo Temple

  • Eom, Young Geun;Kim, Hwa Sung;Xu, Guang Zhu
    • Journal of the Korean Wood Science and Technology
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    • v.33 no.2 s.130
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    • pp.1-7
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    • 2005
  • Tree species of wooden structural members of the Beomeo Temple were identified based on light and scanning electron microscopic characters in the present study. Of 10 structural members, 9 softwoods and 1 hardwood were identified. Among softwood members, 7 belonged to hard pine of the Sylvestris section, and the remaining 2 to hard pine of other than the Sylvestris section and hemlock of the genus Tsuga, respectively. A single hardwood member was identified as white oak of the Prinus section under the subgenus Lepidobalanus.

Early Begetational Recovery and Species Diversity of Pine Forest after Fire in Mt. Todok (山火後 道德山 소나무林의 初期植生回復과 種多樣性)

  • Cho, Young-Ho;Woen Kim
    • The Korean Journal of Ecology
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    • v.14 no.1
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    • pp.15-23
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    • 1991
  • The early vegetational recovery and species diversity were investigaed in burned pine forest of Mt. Todoksn from 1986 to 1990. The changes of vegetation in the burned site occurred Miscanthus→Miscanthus-Lespedeza→Lespedeza community. The biological spectra based on SD(%) and SP(%) of the burned site were similar to the unburned site with H---e type. The degree of succession(DS) was gradually increased with succession in burned site. The species diversity index(H') and evenness index(e) were declining successional trend, nut dominance index(C) inclining successional trend in the burned site. The domiance-diversity curves of the burned site showed gradual decrease in diversity and evenness, and then slopes of them were progressively steep in early successional stage.

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Vegetation Change after A Forest Fire in a Rural Japanese Red Pine Forest and Applications of Effective Microorganism (농촌 소나무림에서 산불에 의한 식생변화와 유용미생물의 적용)

  • Yeo, Ji Sean;Kim, Kee Dae
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.11 no.4
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    • pp.46-56
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    • 2008
  • This study examined the natural restoration of vegetation through monitoring of the development of a vegetation community from 2006 through 2007 after a forest fire. Approximately 5,000 $m^2$ in a forest near Topyeon-ri, Kangnae-myeon, Chungcheongbuk-do with Japanese red pine (Pinus densiflora) forest and its floor vegetation had been completely burned by a fire in April 2005. This area and another nearby Japanese red pine forest were selected as the experiment site and the control site, respectively. Vegetation survey was conducted at the experiment site and the control site. A seed bank experiment was carried out in the greenhouse to examine underground vegetation. Effective microorganism(EM) was applied to the seed bank experiment to estimate its effects on the direction of ecological succession. According to the results, a total of 36 plant species including shrub and herbaceous species were discovered in the experiment site. Quercus serrata, Lespedeza cyrtobotrya, and Castanea crenata, Rubus crataegifolius, Oplismenus undulatifolius, and Carex lanceolata were among the most abundant species. Biomass in the experiment site reached 2.4 times biomass than those in the control site, indicating the productivities of shrub and herbaceous layers are better in the experiment site. According to the result of the soil seed bank experiment of the experiment site, a total of 182 plants of 14 species were recorded. In addition, a total of 13 plants of 2 species were found from soil seed bank of the experiment site applied by EM. If EM is applied to the burned site, it will control the budding of herbaceous plants, creating the gap between herbaceous plants. This loss of competition is expected to help the restoration of trees in the burned area.