• Journal of Korean Society of Forest Science
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• v.32 no.1
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• pp.16-20
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• 1976

Employing 7-15 resistant and 8-15 susceptible Japanese black pine (Pinus thunbergii Parl.) trees to pine gall midge (Thecodiplosis japonensis Uchida et Inouye) as samples, needle monoterpenes were analysed by GLC in January and June, and observation was made on the oviposition preference. Following results were obtained. 1. In January, the resistant trees showed higher contents of myrcene, limonene, ${\beta}$-phellandrene and terpinolene but lower contents of ${\alpha}$-and ${\beta}$-pinene and camphene compared to the susceptible trees. But in June, ${\alpha}$-pinene, camphene, limonene, ${\beta}$-phellandrene and terpinolene were higher and the content of ${\beta}$-pinene and myrcene were lower in the resistant trees than the susceptible trees. 2. The content of limonene was higher by the 6.8 percent and the content of ${\beta}$-pinene was lower by the 9.2 percent in the resistant trees than in the susceptible trees in June. 3. No preference for oviposition was found between resistant and susceptible trees. But in the resistant trees gall formation rate was quite lower than the susceptible trees. It was considered, therefore, that limonene and ${\beta}$-pinene content in the needle might be used as an indicator of the resistant Japanese black pine to the pine gall midge regardless of season.

• Journal of Korean Society of Forest Science
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• v.65 no.1
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• pp.48-53
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• 1984

Using the Duff and Noland's method, the annual ring growth patterns of the red pine in the natural stands were examined at two locations; Seocheon, Chungnam-do where the stand had been infested with the pine needle gall midge, Thecodiplosis japonensis, during the years from 1975 to 1978, and Hongsong, Chungnam-do where had been no incidence of the insect damage. The results obtained are as follows: 1) With the normal red pine of 13 year old, the growth pattern in the oblique sequence indicates that the annual growth rates are maximum at the few terminal internodes, and decrease gradually with the downward internodes. Such characteristic of the growth pattern is not clear in the horizontal sequence of annual rings. 2) The indications of the radial growth reduction of the pine tree due to the pine gall midge infestation could be examined with any of three series at the crown level internodes; horizontal, vertical and oblique series. For the basal internodes, however, the horizontal series appeared to be inadequate for the analysis of the damage impact because it seemed to be masked by various factors other than direct effects of the damage. 3) Of the three ways of radial growth analyses, oblique sequence seems to be the most usefull, especially for the detection of the impact on growth caused by incipient or light infestation.

• Journal of Korean Society of Forest Science
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• v.83 no.3
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• pp.311-321
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• 1994

Experiments were conducted to evaluate the efficacy of ground and foliar sprinkling of natural water for control of the pine needle gall midge, Thecodiplosis japonensis, by disturbing the reproductive behavior of adults such as copulation and oviposition. Diurnal ground spray for whole area application and foliar spray for single tree treatment were tested at day time during the period of adult emergence. The results obtained are as follows : 1. The population density of overwintering larvae in soil was not affected by the water spray during the adult emergence period. 2. Spraying the water showed no effect on the whole period and number of adult emergence, but retarded early emergence and facilitated Late emergence. The difference in the emerging time by 50% emergence day was approximately 7 days. 3. Ground spray was significantly different from control in gall formation rates but was not sufficient for effective control. As a single-tree treatment, the foliar spray was found to be highly effective for control of the pine needle gall midge, Average gall formation of spray plots and untreated ones were 2.6% and 38%, respectively ; control value exceeded 90%. 4. Ground spray of water was not effective in reducing larvae in gall of needle. Foliar spray, however, reduced 45% of larval numbers. 5. Foliar spray accelerated shoot growth by 15%-18%. 6. Foliar spray was highly effective for the control of sucking insects, such as mites and aphids on pine trees.

• Journal of the Korean Wood Science and Technology
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• v.23 no.1
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• pp.49-53
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• 1995

The terpenoids are one of the secondary metabolities in Pinus species and functioned the protection them from insects or/and fungus etc. Since Pinus koraiensis has not any damage by Pine needle gall midge but Pinus densiflora has great injury by them in South Korea. It may great helf to find these reasons to analize their monoterpenenoids and to compare the seasonal variation of their amounts. Terpenoids were analized by GC, GC/MS. Some biogenetic analysis of terpenoids also was elucidated. The seasonal variation of terpenoid was distinguished at March and August and/or September. Most of major component of monoterpenes were increased or decreased in these periods in Pinus koraiensis. Especially 3-carene was showed significant variation between the period of physiological activity and the period of domain. The effect of infection by Pine needle gall midge was significant in Pinus densiflora for amount of monoterpene during the period of infection. The components of p-menthane series between Pinus densiflora and Pinus koraiensis was recognized a significant difference during the experiment. Biogenetic cooccurrance of pmenthane series also was recognized between 3-carene and terpinolene in Pinus koraiensis.

• Journal of Korean Society of Forest Science
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• v.87 no.4
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• pp.571-576
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• 1998

Experiments were conducted to evaluate the efficacy of the application of low concentration insecticides by sprinkler system for control of the pine needle gall midge, Thecodiplasis japonensis, by killing the adults emerging and copulating near the ground. For ground spray, 2 insecticides of fenitrothion and deltamethrin, dilutions of $2,000{\times}$ and $3,000{\times}$ deltamethrin, and 3 spray intervals of 1-3 day were tested at the peak time of adult emergence during the day time. The results are as follows ; 1. Ground spray of fenitrothion 50% EC and deltamethrin 1% EC were found to be highly effective for control of the pine needle gall midge. Average gall formation by fenitrothion, deltamethrin and untreated ones were 3.40%, 5.23% and 45.69%, respectively ; control value of both insecticides exceeded 88%. 2. As a ground spray of deltamethrin 1% EC, dilution $2,000{\times}$ was significantly different from $3,000{\times}$ in gall formation rates. Average gall formation of $2,000{\times}$ plots and $3,000{\times}$ treated ones were 5.23% and 18.00% ; control values were 89.44% and 63.66%, respectively. 3. Diurnal treatment of ground spray was found to be highly effective for control of pine needle gall midge. 4. In particular, control by ground application of dilutions $2,000{\times}$ and $3,000{\times}$ of deltamethrin is suggested because it does not have an adverse effect on arthropods of the ground surface such as ants and spiders.

• Korean journal of applied entomology
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• v.19 no.1 s.42
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• pp.5-9
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• 1980

Some insecticides were evaluated on the effect of single ULV foliar spray in the control of the pine gall midges (Thecediplosis japonensis Uchida et Inouye) when the formulated (undiluted) and/or diluted insecticides were applied by ULV Sprayer (Battery-type of 12 voltage, devised by Union Carbide) With the formulated insecticide spray (30ml per plot; ten pine trees of 1.5 to 2m in height), the order of control effectiveness was Salithion (Ec 25), $Sumithion^{(R)}$ (ULV 80), Dimethoate (Ec 50), $Sevin\;oil^{(R)}$ (ULV 50), $Zolone^{(R)}$ (Ec 25) and $Folimat^{(R)}$ (Ec 50). However, except Zolone, other insecticides tested caused relatively severe phytotoxicity on the pine needles in all treatments. The dilluted insecticides (200ml Per Plot) of Salithion and Dimethoate with 10,20 and 40 times of water solution showed better control effect than with the formulated insecticides, and no phytotoxicity was observed. Salithion was more effective than Dimethoate. In conclusion, the desirable results in the pine gall midge control in this experiment were obtained by single ULV foliar spray of Salithion with 10 to 20 times of water solution, and the feasible timing of insecticide application would be from late in May to early in June.

• Korean journal of applied entomology
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• v.18 no.4 s.41
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• pp.153-160
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• 1979

The present experiments were carried out to reduce the population density of adult pine gall midge, Thecodiplosis japonensi,s UCHIDA et INOUYE, by means of spray the sticky agent C-4, on the ground, herbacious plantation and foliar leaf of pine trees at Sanbonli,. Anyang, Kyungido. And also the 3 periods of treatment, such as before 2 weeks( I ), before 1 week (II) from the peak emergence period and peak emergence period (III) were applied. The experimental results obtained were as follows. (1) The formula of selected sticky agent C-4 was $70\%$ of castor oil, $25\%$ of damar resin and $5\%$ of carnauba wax, and it showed the best both on stickiness and duration, and lower phytotoxicity to the host plant. (2) The reduction of population densities of the adult PGM were $68\%$ and 78f: in the G-I and G-II treatment plot respectively, which compared to control, on the ground spray. (3) And the reduction of population densities were $63\%$ and about $90\%$ in the P-I and P-II plot respectively when the agent was sprayed on the herbacious plantation. (4) The rates of gall formation were $32.8\%,\;40.8\%\;and\;59.4\%$ in the spray plots of F-I, F-II, and F-III respectively, and there was no significant difference among the upper, middle and lower parts of the treated host plant in the rates of gall formation. (5) The effective stage of sticky agent application were considered as before one week from the peak emergence period in tile all types of treatment.

• Microbiology and Biotechnology Letters
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• v.27 no.5
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• pp.415-418
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• 1999

Thecodiplosis japonensis is an important insect pest in pine trees and hard to be controlled by chemical pesticides due to its characteristic life cycle. Experiments were conducted to determine the pathogenicity and growth temperature of several entomopathogenic fungi as potential microbial insectcides for T. japonensis. Beauveria bassiana SFB-SC and Paecilomyces fumosoroseus SFP-198 were highly pathogenic against T. japonensis. B. bassiana SFB-SC had also pathogenicity against a beneficial insect Bombyx mori. Growth rate and pathogenicity of P. funmosoroseus SFP-198 under 15$^{\circ}C$ was higher than those of B. bassians SFB-SC. These results indicated that P. fumosoroseus SFP-198 is a potential microbial control candidate for control of T. japonensis.

• Korean journal of applied entomology
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• v.15 no.4 s.29
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• pp.169-178
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• 1976

The concentration of amino acids, total nitrogen, trehalose, lipids and the activities of respiratory, acid$\cdot$alkaline phosphatase, glutamic oxalozcetic transaminase and glutamic pyruvic transaminase during larval stage in Pine leaf gall midge, Thecodiplosis janensis Uchi. et Inouye were measured using Paper chromatographic method, micro-Kjeldahl method, Thin layer chromatographic method, Warburg's manometric method, Bessey-Lowry method and Reitman-Frankel method, respectively. Healthy specimens )yore chosen as samples of each larval stages; alrva in gall and larva in soil. Amino acids present in the alcoholic extracts were alanine, glutamic acid, glycine, histidine, methionine, proline, threonine, tryptophan and valine. The total nitrogen concentration reached to 31.348mg/g during the larva in gall and the larval stage in soil of the value was decreased to 29.027mg/g. The hemolymph sugar, trehalose value for larva in soil was about two times of the value for larva in gall. Total lipid, phospholipid,monoacylglycerol, triacylglycerol, sterol, free fatty acid and ester cholesterol were identified at larval stages in gall and soil. Triacylglycerol concentration reached high level in contrast with other lipid contents during larvae in gall and larva in soil. Free fatty acid, sterol except decreased lipids during larval stage in soil. Endogenous respiration, succinate of respiratory activities decreased at larval stage in soil compare with larva in gall. The activities of acid phosphatase decreased larval stage in soil but the activities of alkaline phosphatase increased remarkably. The activities of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase reached high level of the larva in gall.

• Korean journal of applied entomology
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• v.25 no.1 s.66
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• pp.1-9
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• 1986

Periodical samplings were made in the fields, located Hwasong-Gun, Banwal-Myon, Doondai-Ri, for two years to study the major factors responsible to the population of the overwintered pine needle gall midge, Thecodiplosis japonensis Uchida et Inouye. The population densities of the overwintered larvae reduced greatly at the time of pupation and the changing patterns seemed to be influenced by environmental conditions. The estimated 50% pupation day was about two weeks earlier for the 1980 generation compared with the 1979 generation, and much higher average and the daily maximum temperatures in 1981 affected on the development of the larvae. The relative emergence rates were 14.1% for 1979 and 14.9% for 1980 generation. The relative emergence rates(Y) were affected by the moisture contents of soil ($X_1$) and its variance $(X_2),\;Y=-68.41+4.3206X_1-0.6887X_2$. The relative emergence rates seemed to be decreased with the increased variance of the moisture contents of soil. Percents of needle gall were 49.63% for 1980 and 86.87% for 1981 generation.