• Journal of Life Science
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• v.23 no.4
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• pp.542-553
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• 2013

The aim of this work was to establish the optimal conditions for the production of cellobiase by a marine bacterium, Cellulophaga lytica LBH-14, using response-surface methodology (RSM). The optimal conditions of rice bran, ammonium chloride, and the initial pH of the medium for cell growth were 100.0 g/l, 5.00 g/l, and 7.0, respectively, whereas those for the production of cellobiase were 91.1 g/l, 9.02 g/l, and 6.6, respectively. The optimal concentrations of $K_2HPO_4$, NaCl, $MgSO_4{\cdot}_{7H2}O$, and $(NH_4)_2SO_4$ for cell growth were 6.25, 0.62, 0.28, and 0.42 g/l, respectively, whereas those for the production of cellobiase were 4.46, 0.36, 0.27, and 0.73 g/l, respectively. The optimal temperatures for cell growth and for the production of cellobiase by C. lytica LBH-14 were 35 and $25^{\circ}C$, respectively. The maximal production of cellobiase in a 100 L bioreactor under optimized conditions in this study was 92.3 U/ml, which was 5.4 times higher than that before optimization. In this study, rice bran and ammonium chloride were developed as carbon and nitrogen sources for the production of cellobiase by C. lytica LBH-14. The time for the production of cellobiase by the marine bacterium with submerged fermentations was reduced from 7 to 3 days, which resulted in enhanced productivity of cellobiase and a decrease in its production cost. This study found that the optimal conditions for the production of cellobiase were different from those of CMCase by C. lytica LBH-14.

• Journal of Korea Port Economic Association
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• v.30 no.1
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• pp.175-194
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• 2014

Because the thawing of the Arctic ocean is slowly accelerating due to global warming, recently exploring resources in Arctic ocean and transporting resources by using the North Pole route have been getting spotlight. Since the original route transported by the Suez Canal from Korea to Europe could be shorten about 8,000km in distance(decreased about 38% compared to the original route), which means shortening about 10 voyage dates, it is expected to bring huge logistics cost reduction. Once the North Pole route is commercialized successfully, it would be one of the most important variables that affects future of Busan port and guides for economic development of Busan. Therefore, the purpose of this study is to analyze Busan port and the economic growth of Busan area by researching promising industry, based on the effect of freight transporting by the Northern sea route on the economy of Busan. For this study, questionnaire surveys and interviews were conducted for 64 people of experts in the shipping and port industry, relevant government, and academics. The survey finding shows that port logistics industry is a promising business in Busan in terms of its growth and competitiveness. It is necessary to develop feeder network facilities that prepare for commercialization of the Northern sea route as a short and medium term plan and provide professional manpower training in polar regions. Ship supply business would also play an important role. It is identified that revitalization of shipbuilding and ocean plant industry should be done in terms of Arctic business. With regard to the fishery industry it is found that modernization of fishery ship and development of fishery equipment used in polar areas should be carried out.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.1
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• pp.27-40
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• 2022

Building self-sustainable rural infrastructure and environment through smart digital agriculture technology innovation is one of the major goals of the Korean agricultural administration as a part of the nation's 4^th industry revolution. To identify areas for improving and effectively investing in the acceleration of rural development, 207 experts in the areas of crop science and smart digital agriculture technology were interviewed for their opinions and suggestions on 22 questions designed to recognize fundamental agricultural issues to be addressed and solutions to advance technology innovation and rural development. Majority of the participants expected smart digital agriculture technologies to resolve major agricultural issues and help build a better rural environment. To overcome technology gaps and resolve issues more effectively, further investment in training new technology experts and building stronger agricultural technology infrastructure is urgent, and persistent and systematic support from agricultural administration appears to be the key for accelerating the process. While the leading global groups of both public and private sectors have advanced their technologies beyond the field application stage, most of the Korean technologies remain at the early pilot stage. Aging population and lack of labor in rural areas, unknown future climate change, and challenges in sustainable rural development are expected to be resolved by smart digital agriculture technologies. Technological innovations by research institutes should be promptly deployed in the crop production field, and farm training systemically organized by local technology centers can accelerate farming revolution. Standardization of equipment and data systems is another key to the success of digitalization of food crop production and food supply chains nationwide.