• Journal of Chest Surgery
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• 제33권3호
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• pp.213-220
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• 2000

Background: Adhesion of leukocytes to myocardium or vascular endothelium has been known as an importation initial step in the ischemia-reperfusion injury which may affect the cardiac function. Therefore, leukocyte-depleted reperfusion may inhibit ischemia-reperfusion induced functional and ultrastructural deterioration. In this study, we quantified the time-dependent expression of the vascular cell adhesion molecule-1(VCMA-1) on piglet myocardium and demonstrated its relation to functional recovery using isolated piglet heart perfusion model. Material and Method: Neonatal(1 to 3 day old) piglet heart was harvested with 4$^{\circ}C$ University of Wisconsin solution (UWS) and presrved in the same solution for 12 hours. Ex vivo model of an isolated working neonatal piglet heart perfusion consisting of membrane oxygenator and roller-pump was used (Fig. 1). Hearts were grouped into leukocyte-non-depleted (group A, n=8) and leukocyte-depleted group(group B, n=8). In group B, hearts were reperfused with leukocyte-depleted blood using a leukocyte filter (Sepacell R, Asahi Medical, Japan). Segments of right atrium were taken before and after 1, 2, 3, and 4 hours of reperfusion for the evaluation of expression of VCAM-1. The intensity of immunohistochyemical satining of the VCAM-1 on the myocardium were graded semiquantitatively (0 to 4). For the evaluation of myocardial stroke work indices were calculated as well at the same time-points. Result: Mean expressins of VCAM-1 on the myocardium at 0, 1, 2, 3, adn 4 hours of reperfusion were 0.63, 1.44, 1.64, 2.65, and 3.34 in group A, while 0.56, 1.40, 1.50, 1.88 and 2.14 in group B (Fig. 3). Mean stroke work indices at 0.5, 1, 2, 3, and 4 hours after reperfusion were 1.35$\times$104, 1.32$\times$104, 1.14$\times$104, 0.81$\times$104, 0.68$\times$104 erg/gm in group A, while 1.40$\times$104, 1.43$\times$104, 1.43$\times$104, 1.28$\times$104, and 1.12$\times$104 erg/em in group B(Fig. 4). Conclusion : In this study, we demonstrated that leukocyte-depletion attenuated the expression of VCAM-1 during reperfusion and the time-dependent functional deterioration of the myocardium was well correlated with the degree of VCAM-1 expression.

• Animal Bioscience
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• 제34권11호
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• pp.1749-1756
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• 2021

Objective: Microminipig (MMP) is a miniature pig with an extra small body size for experimental use. In the present study, the occurrence of stillbirths and their genetic association with swine leukocyte antigen (SLA) class II haplotypes were evaluated in a population of MMPs. Methods: The occurrences of stillbirth and genetic association with SLA class II haplotypes using 483 stillborn and 2,246 live piglets, and their parents were compared among the three groups of newborn piglet litters; an all stillborn (AS) group consisting of only stillborn piglet litters, a partial stillborn (PS) group consisting of stillborn and live piglet litters, and an all alive (AA) group consisting of only live piglet litters. Results: The incidence of stillborn piglets was 483/2,729 (17.7%). Distributions of litter sizes, numbers of stillborn piglets in a litter, parities, and gestation periods were distinct among the three groups. The frequencies of low resolution haplotype (Lr)-0.7 or Lr-0.23 were higher in the AS group than in the PS or AA groups. In sires, the frequency of Lr-0.7 associated with the AS group was significantly higher in the AS group than with the AA group. In dams, the frequency of Lr-0.23 was significantly higher in the AS group than in the PS or AA groups, whereas the frequency of Lr-0.7 was not significantly different. Conclusion: The incidence of stillborn piglets in MMPs appears to be higher than those in other pig breeds. Several traits related with stillbirths such as the number of stillborn piglets and parities of the AS group were different from those of the PS and AA groups. Specific SLA class II haplotypes were associated significantly with a high incidence of stillbirths and could be used as genetic markers to adopt breeding strategies to lower the rate of stillbirth in MMPs.

• Animal Bioscience
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• 제35권11호
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• pp.1733-1743
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• 2022

Objective: This study was aimed to explore the efficacy of combination of endo-xylanase (Xyn) and xylan-debranching enzymes (arabinofuranosidase, Afd and feruloyl esterase, FE) in improving utilization of bran in piglet diet. Methods: In vitro experiments were firstly conducted to examine the enzymological properties of Xyn, Afd, and FE, concurrent with their effect on degradation of arabinoxylan (Abx) in bran. In vivo experiment was then implemented by allocating two hundred and seventy 35-d-old postweaning piglets into 3 groups (6 replicates/group), which received bran-containing diet supplemented with Xyn (1,600 U/kg) or its combination with Afd (0.8 U/kg) and FE (4 U/kg) or without enzyme. Results: Both Xyn, Afd, and FE are relatively stable against the changes in temperature and pH value. Combining Xyn with Afd and FE had a superiority (p<0.05) over Xyn alone and its combination with Afd or FE in promoting (p<0.05) degradation of Abx in different brans. Combined treatment with Xyn, Afd, and FE was more beneficial than Xyn alone to induce increasing trends (p<0.10) of average daily gain, final body weight and feed efficiency of piglets fed bran-containing diet. Moreover, combination of Xyn, Afd, and FE showed advantages (p<0.05) over Xyn alone in causing reductions (p<0.05) in diarrhea rate and cecal pH value, concurrent with increases (p<0.05) in cecal and colonic acetic acid and total volatile fatty acid concentrations, as well as cecal butyric acid concentration of piglets fed bran-containing diet. Conclusion: Combining Xyn with Afd and FE was more beneficial than Xyn alone in promoting degradation of Abx in bran, along with growth performance and intestinal volatile fatty acid profile of piglets received bran-containing diet. Thereby, combination of Xyn, Afd, and FE had a superior efficacy relative to Xyn alone in improving application of cereal bran in piglet diet.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 춘계학술대회
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• pp.170-170
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• 2002

• 대한수의학회지
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• 제27권2호
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• pp.259-267
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• 1987

Enterotoxigenic E. coli (ETEC) cause an acute diarrhea (white scour) in both animals and humans. The disease process initially involves the adherence and colonization of the mucosal surface of the small intestine, followed by the elaboration of a heat-labile enterotoxin (LT) and/or heat-stable enterotoxin (ST). Intestinal adherence or colonization by ETEC is generally mediated by a specific surface-associated pilus (fimbrial) antigen that endows the bacteria with the capacity to adhere to epitherial cell surface. Fourteen monoclonal antibodies (MAbs) directed against pili antigens of ETEC were obtained by cell fusion/hybridoma technique. They were characterized by indirect immunofluorescence assay (IFA), and divided into four groups: specific to K99 antigen (group 1), cross-reactive with K99 and F41 antigens (group 2), specific to K88 antigen (group 3) and specific to 987P and K88 antigens (group 4), respectively. These MAbs demonstrated the distinct pili (K) antigens on the surface of ETEC by IFA, and could be utilized as diagnostic reagent for the identification of ETEC. When eighty-seven field isolates of E. coli from piglet with diarrhea were tested by group 3 MAb, fourty-two strains (48.3%) has K88 pilus antigen suggesting that this is one of the major pilus antigen of ETEC present in fifeld.

• 한국축산시설환경학회지
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• 제19권2호
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• pp.141-148
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• 2013

This study was conducted to evaluate the effect of different housing systems on the performances of sows and their piglets during gestation. A total of 90 sows (Landrace ${\times}$ Yorkshire) were employed into 3 experimental farms to give 3 treatments, stall housing, group housing in either slatted floor or litter floor. Individual sow was used as an experimental unit. Group housed sows were fed with electronic sow feeder during gestation. Performance measures were taken on sows and piglets. Back-fat thickness and body condition score of sows were not affected by housing systems for pregnant sows. There was no difference of estrus interval of pregnant sows between housing systems. The lower number of still-birth was observed in group housing type. The number of wounded sows in slatted floor was remarkably increased compared with sows in litter floor. This study showed that the housing systems could fairly impact sow and piglet performances.

• 한국가축번식학회지
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• 제3권2호
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• pp.50-56
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• 1979

This experiment was conducted to clarity the possibility of practical use of farrowing inductionin sow by the administration of prostaglandin F2$\alpha$. For this experiment, total 320 heads of pregnant sow and its piglets were used. The reproductive characteristics of artificially farrowed sow and, health and growth state of piglets were estimated. The results obtained in this experiment were summarized as following: 1. No significant difference were observed between naturally and artifically farrowed sow in several aspects such as the rate of dystocia, length of farrowing, farrowing intervals from piglet to piglet. 2. significant (P<0.05) differences were observed between naturally and artificially farrowed sow in intervals from weaning to estrus. However, there were no significant differences among those of 5, 7.5 and 10mg treated group. 3. There were no differences in number of stillbirth, immature birth and alive piglets at 3 weeks age per litter were observed. 4. Similar birth weight, weaning weight, daily gain and rearing rate of piglets were obtained from both naturally and artificially farrowing.

• Reproductive and Developmental Biology
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• 제30권3호
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• pp.163-167
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• 2006

The purpose of the present study was to establish culture conditions for the in vitro study of the neonatal piglet Sertoli cell. Isolation for the culture of Sertoli cell was established using collagenase and pancreatin digestion of testicular tissues. The effects of various culture media, fetal bovine serum(FBS), follicular stimulating hormone(FSH), epidermal growth factor(EGF) and insulin-transferrin-sodium selenite(ITS) on growth of neonatal piglet Sertoli cells were investigated. The mitogenic effects of Dulbecco's modified Eagle's medium+Ham's F-12 medium was higher than other media used in this experiment. The addition of 1% FBS in cultures was necessary for attachment of Sertoli cell clusters. However, except FBS and EGF, FSH and ITS did not stimulate Sertoli cell proliferation. When Sertoli cells isolated from neonatal piglets were cultured in Dulbecco's modified Eagle's medium+Ham's F-12 medium supplemented with 1% FBS, FSH EGF and ITS, the yield and plating efficiency of Sertoli cells were largely increased. Confluency of Sertoli cells was reached as early as 4 days of culture. The method described here reduces or eliminates many of the drawbacks of the conventional procedures used to isolate and culture of Sertoli cells, thus providing a useful tool in studies of growth kinetics and regulation of cell proliferation in vitro.

• 미생물학회지
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• 제53권2호
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• pp.134-136
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• 2017

이유자돈용 생균제 개발을 위해, 본 연구자들은 유산균의 일종인 Lactobacillus salivairus KLW001 균주를 대한민국 양돈가에서 사육 중인 이유자돈으로부터 분리하였다. 이 균주는 K88 antigen-positive Escherichia coli, Salmonella enterica serovar Typhimurium에 대한 항균 활성이 타 균주보다 우수하여, 우리는 이 균주의 유전체를 분석하였다. 유전체 초안 속의 166개 Contig (${\geq}500bp$)들에서, G+C content (%)가 33.0%였고, 2,326,706 bp 크기의 염기서열을 확보할 수 있었다. 유전체 초안으로부터 항생제 저항 유전자 4개, Phage 관련 유전자 36개, Bile 대사 유전자 3개, CRISPR Spacer 27개를 확인하였다.

• 농업과학연구
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• 제48권2호
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• pp.191-199
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• 2021

Salmon oil (SO) could be used as a great source of ω-3 fatty acids in pig diet. The purpose of the study was to investigate the SO effect on production performance of sows and their offspring. 48 lactating sows (Landrace × Yorkshire) from Dankook University experimental farm were used in this study. Sows were conceived using either guided natural mating or by artificial insemination. Pregnancy diagnosis was confirmed by ultrasonography on day 25 after mating. Sows and their offspring were assigned randomly to 1 of 2 treatments. Treatments included: 1), control (CON); 2), control + SO 0.5% (CS). The inclusion of SO did not affect (p > 0.05) the litter size throughout the experiment. No difference (p > 0.05) was observed on the average daily feed intake (ADFI), chest circumference, estrus interval and sow backfat thickness between CON and SO treatments. Dietary SO supplemented diet reduced (p < 0.05) body weight loss during lactation compared with control treatment. Pigs fed SO supplemented diet did not affect (p > 0.05) the fecal scores during lactation compared with those fed control treatments. Sows fed SO supplemented diet led to a higher (p < 0.05) initial weight, weaning weight and average daily gain of piglets than those fed control diets. No difference (p > 0.05) was observed on piglet survival and fecal scores throughout the experiment. Inclusion of SO could reduce the body weight loss of sows and improves piglet growth during lactation, indicating SO has beneficial effects for pigs.