• Title/Summary/Keyword: piaA

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The Design of General Purpose Data Acquisition System (마이크로 프로세서에 의한 측정기)

  • Myoung-Sam Ko;Wook-Hyun Kwon;Dong-Il Kim
    • The Transactions of the Korean Institute of Electrical Engineers
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    • v.32 no.9
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    • pp.305-314
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    • 1983
  • This paper presents a general purpose data acquisition system based on the microprocessor system with M 6800. instrument and lograrithmic amplifiers and A/D converters are used to implement a signal conditioner for a various kinds of signals. The proposed system has a function such that the processor may select the one of the input signals as will and also it is proved that the system may control the control signal and digital converted signal through I/O port of PIA. Practical measurement by the proposed system shows in good results.

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Regulation of Insulin-Sensitive Cyclic Nucleotide Phosphodiesterase in Adipocytes of Streptozotocin-Induced Diabetic Rats (Streptozotocin으로 당뇨병을 유발시킨 흰쥐의 지방세포에서 일어나는 Insulin-Sensitive Phosphodiesterase의 조절에 관한 연구)

  • Park, Kyung-Sun;Lee, Myung-Soon;Kim, Kyung-Hwan
    • The Korean Journal of Pharmacology
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    • v.29 no.2
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    • pp.253-261
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    • 1993
  • Possible changes in the role of insulin-sensitive cyclic nucleotide phosphodiesterase(PDE) in mediating the antilipolytic action of insulin were investigated in adipocytes from streptozotocin-induced diabetic rats. Isolated adipocytes prepared from epididymal adipose tissue were incubated, with or without insulin, at $37^{\circ}C$ for 15 min following pretreatment with various drugs or toxins, and three (plasma membranes, microsomal membranes, and cytosol) fractions prepared by differential centrifugation were then assayed for cAMP phosphodiesterase activity. The PDE activities only in the crude microsomal (P2) fractions were activated by insulin both in diabetic and control rats. The basal PDE activities in P2 fractions of adipocytes from diabetic rats were higher than those from control rats, although the maximal effects observed at 2 nM of insulin, $100\;{\mu}M$ of isoproterenol or the combination of both were not significantly different from each other. The insulin-stimulated PDE activities in P2 fractions of adipocytes from diabetic rats were not changed by PIA, a $A_{1}$ adenosine receptor agonist, whereas they were decreased to the basal PDE activities in those from control rats. In addition, the adipocytes from diabetic rats showed an increased sensitivity to pertussis toxin compared to those from controls. There were no differences between diabetic and control rats in the sensitivity of adipocytes to cholera toxin. These data indicate that the impaired signalling through inhibitory receptors such as adenosine receptors in adipocytes from streptozotocin-induced diabetes relates to the loss or the decreased function of $G_i$ proteins, and leads to the increased activity of the insulin-dependent PDE at the basal states.

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Analysis of the ITS (Internal Transcribed Spacer) Region of Opuntia ficus-indica (백년초선인장의 ITS(internal transcribed spacer) 유전자 분석)

  • In Jun-Gyo;Lee Bum-Soo;Kim Eun-Jeong;Choi Kwan-Sam;Han Seung-Ho;Shin Cheol-Woo;Yang Deok-Chun
    • Korean Journal of Plant Resources
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    • v.19 no.1
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    • pp.161-168
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    • 2006
  • To investigate the origin of backyeoncho (Opuntia ficus-indica var. saboten), we isolated 685 bp clone using ITS primer pairs. The rDNA consists of the genes coding for the partial 54 bp 185, 162 bp 5.8S, and partial 56 bp 26S. The coding regions are interrupted by two internal transcribed spacers, 193 bp ITS1 and 220 bp ITS2. The ITS2 of backnyeoncho in length was shorter than that previously registered in Cucurbitoideae plants. The GC contents was 66.8% in ITS1, and 67.7% in ITS2. The rDNA of backnyeoncho matched to the previously reported genes and showed a high similarity with the 95% identity with Pereskiopsis porteri (L708037). In the phylogenetic analysis, the backnyeoncho rDNA was clustered with Pereskiopsis porteri (L708037).

A MOLECULAR SIMULATION STUDY ON BETA-CYCLODEXTRIN POLYMERIC MEMBRANES

  • Tocci, Elena;Fama, Angelo;Perrone, Maria Pia;Russo,Nino;Drioli, Enrico
    • Proceedings of the Membrane Society of Korea Conference
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    • 2003.07a
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    • pp.57-59
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    • 2003
  • Molecular dynamics simulations have been performed on $\beta$-cyclodextrins octyl-derivative (b-CD) encapsulated into a polymer matrix of glassy poly(ether ether ketone) (PEEK-WC) material to investigate the effects of the complexation of p-nitrophenilacetate and naringin molecules with the aim to study the recognition properties of b-CD.

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Analysis of the Inundation Potential by Elevation for the Land Evaluation in the Potentially Inundated Farms - A Case Study in Ibang-myeon, Changnyeong-gun, Kyungsangnamdo - (상습침수 농경지의 토지평가를 위한 고도별 침수 잠재성 분석 - 경상남도 창녕군 이방면을 대상으로 -)

  • Park In-Hwan;Jang Gab-Sue;Seo Dong-Joe
    • Journal of the Korean Institute of Landscape Architecture
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    • v.33 no.2 s.109
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    • pp.71-82
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    • 2005
  • A large scale of riverside rearrangement has been recently done in the major rivers in Korea. So inundation possibility in agricultural area closed by these rivers has been higher than the possibility a few years ago. However, land use in this area has not been adjusted to a change of this situation near the rivers. Therefore, when typhoon or heavy rain is happened on this area, it can cause a large damage in agricultural area. This study analyzed inundation potentiality in agricultural area at Ibang-myeon, Changnyeong-gun, Kyeongnam-province, Korea by using the logistic regression model and the piecewise regression model. The first thing we did was to transfer the inundation area per elevation to the accumulated inundation area per elevation. This accumulated inundation area per elevation as an distribution function could be described by the logistic regression model(LRM), and piecewise regression model(PRM) could make it much more accurate to analyze the inundation area per elevation. As a result, the regression models derived from LRM and PRM showed $R^2$ over 0.950. The models derived from LRM and PRM in Ibang-myeon noted that frequently inundated area(FIA) was shown up to 12.12m in elevation, and potentially inundated area(PIA) was shown up to 14.60m in elevation. In FIA, regular agricultural activity would be impossible. And It would be not easy to continue the regular agricultural activity in PIA. So, this land should be rearranged to be used for a buffer zone for ecosystem protection, landscape conservation and things like that in riverside.

Hydrolysis of Phosphatidyicholine to Initiate HeLa Cell Adhesion to a Gelatin Substratum (Phosphatidylcholine의 분해에 의한 Hela 세포와 Gelatin 기질과의 상호작용의 유도)

  • ;;;;;Bruce S. Jacobson
    • The Korean Journal of Zoology
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    • v.38 no.4
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    • pp.457-464
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    • 1995
  • Hela cells, a transformed human epithelial cell line, attach to various substrata but subsequent spreading is specific to collagen or gelatin. The spreading is initiated by the activation of phospholipase $A_2$ (PLA$_2$) which produces arachidonic acid (AA) as a consequence of cell surface collagen receptor clustering. This study examines the mechanism of PLA$_2$activation and which phospholipids are hydrolyzed by PIA$_2$ to release AA in response to Hela cell adhesion to a gelatin substratum. The levels of phosphatidyicholine decreases, among various phospholipids, during attachment and spreading of Hela cells. Lysophosphatidyicholine Is the only lysophospholipids formed during ileLa cell adhesion indicating that clustered collagen receptors activate PLA$_2$to hydrolyze posphatidylcholine to AA and lysophosphatidylcholine. Among various molecular entitles which are known to regulate PLA$_2$ activation, we have previously shown that PLA2 activation is not mediated by either changes in $Ca_2$+ levels, alkalinization of cytoplasmic p11, or activation of protein hinase C. It is also likely that PIA2 activation is not mediated by either pertussis or cholera toxinsensitive G proteins as those toxins do not affect both AA release and cell spreading.

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Effects of Antiiflammatory Agents on Acetaldehyde Induced Cytotoxicity (Acetaldehyde 유도 세포독성에 대한 항염증제의 영향)

  • 이수환;이병훈;김강석;문창규
    • Journal of Food Hygiene and Safety
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    • v.8 no.3
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    • pp.157-161
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    • 1993
  • In order to get infonnations on the development of alcohol induced cardiovascular disorders, primary cultured vascular smooth muscle cells (PVSMC) were treated with acetaldehyde, one of the most reactive metabolites of ethanol. Acetaldehyde caused the striking release of lactate dehydrogenase (LDH) from PVSMC and it stimulated the prostaglandin synthesis in the same system. But it didn't induce cyclooxygenase activity. lipoxygenase inhibitors-propyl gallate and nordihydroguaiaretic acid could reverse the effect of acetaldehyde, but dexamethasone, a phospholipase $A_2\;(PIA_2)$ inhibitor and cyclooxygenase inhibitors except indomethacin could not protect the cells from acetaldehyde toxicity. These results indicate that enhanced prostaglandin synthesis by acetaldehyde is not a direct cause of cell death, but secondary effect due to the activation of PIAl and also, the roles of the lipoxygenase metabolites and/or $PIA_2$ activity itself might be more important in the cytotoxicity of acetaldehyde.

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Molecular Cloning of a cDNA Encoding Ribulose-1,5-bisphosphate Carboxylase Small Subunit (rbcS) from Panax ginseng C. A. Meyer (고려인삼의 Ribulose-1,5-Bisphosphate Carboxylase Small Subunit(rbcS) 유전자의 분리 및 특성분석)

  • In Jun Gyo;Lee Bum Soo;Youn Jae-Ho;Son Hwa;Yi Tae Hoo;Yang Deok Chun
    • Korean Journal of Plant Resources
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    • v.18 no.3
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    • pp.374-381
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    • 2005
  • A full-length cDNA encoding ribulose-1,5-bisphosphate carboxylase small subunit (rbcS) has been isolated and its nucleotide sequence determined from root in ginseng plant (Panax ginseng). The rbcS cDNA of ginseng is 790 nucleotides long and has an open reading frame of 549 bp with deduced amino acid of 183 residues (pI 8.37), 20.5 kDa. The deduced amino acid sequence of rbcS matched to the previously reported rbcS protein genes and showed a high similarity with the $78\%$ identity with rbcS of Helianthus annuus (CAA68490). In the phylogenetic analysis based on the amino acid residues, the ginseng rbcS was clustered with H. annuus (CAA68490), C. morifolium (AA025119) and L. sativa (Q40250).

Personal Information Protection by Privacy Impact Assessment in Information System Audit (정보시스템 감리에서 개인정보 영향평가를 통한 개인정보 보호)

  • Kim, Hee-Wan;Ryu, Jae-Sung;Kim, Dong-Soo
    • The Journal of the Korea Contents Association
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    • v.11 no.3
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    • pp.84-99
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    • 2011
  • As the integrated and large-scale information is extended due to an advanced information system, a possibility of leaking out privacy increases as the time passes by. As a result, the necessity of using a privacy impact assessment (PIA) is emphasized because it can analyze and minimize the element of invasion of privacy. However, an essential audit for personal information protection is not fulfilled because most of the information system audit supervises over physical, managerial, and technical security items of system architecture area so that general items are the only things being checked. Consequently, this paper proposes that in order to minimize the invasion of personal information, the privacy impact assessment should be done. It also presents a procedure and method of personal information protection audit according to the result of the assessment. After applying the suggested method to two projects, it was confirmed that the improvements for protecting personal information were drawn from this paper.

Conversion of Brown Materials, Crude Lipids, Crude Proteins and Aromatic Compounds of Changed Ginseng by 9 Repetitive Steaming and Drying Process (인삼의 구증구폭(九蒸九曝)에 의한 갈변물질, 조지방, 조단백 및 향기성분의 변화)

  • Kim, Do-Wan;Lee, Yun-Jin;Min, Jin-Woo;Lee, Bum-Soo;In, Jun-Gyo;Yang, Deok-Chun
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.22 no.2
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    • pp.333-339
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    • 2008
  • Korean ginseng (Panax ginseng C. A. Meyer) has been used as an important medicinal plant in the Orient for a long time. It has been claimed that ginseng has many beneficial bioactive effects on human health, such as antitumor, antistress, antiaging and enhancing immune functions. Red ginseng possibly have new ingredients converted during steaming and dry process from fresh ginseng. Kujeungkupo method which means 9 repetitive steaming and drying process was used for the processes of green tea, Polygonatum odoratum, and Rehmanniae radix preparata. In this study, ingredient conversion of ginseng by 9 repetitive steaming and drying process were investigated measuring conversion efficiency of brown materials, crude lipids, crude proteins and aromatic compounds. Brown materials, as an antioxidant, in red ginseng were produced through non-enzymatic reaction by heat. Repetitive steaming and drying treatments on ginseng root contiunously increased the content of brown materials and the chromaticity. Crude lipids were degraded by heat and converted into volatile aromatic ingredients. Crude lipids were degraded and decreased by 0.52% after the 5th and 7th. Crude proteins were also decomposed and converted to amino acid. Crude proteins after the 9th treatment were decreased by more than 85% as increased times of treatments. A bicyclogermacrene as aromatic material was decreased as increased treatment times, while but a aromatic caramel was increased.