• Proceedings of the Turfgrass Society of Korea Conference
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• 2002.02a
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• pp.3-5
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• 2002

This study was carried out to get better understandings about morphological, ecological, and genetical characteristics of annual bluegrass collected from different golf courses in Korea and eventually to establish a successful control strategy. Twenty five local lines of annual bluegrass collected from 20 golf courses in Korea were classified into annual or perennial type on the basis of morphological characteristics. Twelve local lines showing obvious morphological differences were selected and then genetically assessed using RAPD analysis. Classification of the 12 local lines through RAPD analysis were considerably similar to that determined by both of morphological differences and phenotype. Responses of the two types of annual blugrass to herbicides were also examined. Shoot growth of annual bluegrass was significantly suppressed by flazasulfuron and the annual type was more susceptible than perennial type, regardless of flazasulfuron concentrations used. By pendimethalin treatment, there was no clear difference in susceptibility between the two types of annual bluegrass. However, by the treatment of dithiopyr, annual type was more sensitive than perennial type in both shoot and root growth. Nine tree species were screened to detect their allelopathic potential on turfgrasses and annual bluegrass. Acacia (Robinia pseudo-acacia) leaves showed selective inhibition in the shoot and root growth as well as their seed germination when treated with 2% and 10%(v/v) of the extract. However, the other leaf extracts except acacia inhibited non-selectively the growth of three turfgrass species such as bentgrass, perennial ryegrass and zoysiagrass and annual bluegrass. The PAL activities of annual bluegrass increased at 24 h after treatment of acacia leaf extract and peaked at 36 h and then decreased till 60h. The highest PAL activity was observed at 36h after treatment of 10%. The highest activity of CA4H in annual bluegrass was observed at 2h after treatment of acacia extract and the level was 4 times greater than that of the control. The phenolic acids such as p-coumaric acid, salicylic acid and ferulic acid were increased with the treatment of acacia leaf extract. The chloroplast membrane and cell wall of annual bluegrass were destroyed by treatment of acacia leaf extract and its inner materials were released. The membranes in annual bluegrass cells might be destroyed by phytotoxic compounds from acacia leaf extract.

• Advances in environmental research
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• v.5 no.1
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• pp.19-35
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• 2016

The phytoextraction of some toxic heavy metals from municipal waste dump soil by castor plant (Ricinus communis) was tested under natural and single or mixed chelant-assisted scenarios in pot microcosms. A sandy loam with total metal contents (mg/kg): Cd (84.5), Cu (114.5), Ni (70.3), Pb (57.8), and Zn (117.5), was sampled from an active dumpsite in Calabar, Nigeria and used for the study. Castor (small seed variety) was grown under natural phytoextraction or single/binary chelant (citric acid, oxalic acid, and EDTA) applications (5-20 mmol/kg soil) for 63 days. Castor exhibited no visual phytotoxic symptoms with typically sigmoid growth profiles at the applied chelant doses. Growth rates, however, decelerated with increase in chelant dose. Post-harvest biomass yields were higher under chelant application than for natural phytoextraction. Both root and shoot metal concentrations (mg/kg) increased quasilinearly and significantly ($p{\leq}0.05$) with increase in chelant dose, furnishing maximum levels as: Cd (55.6 and 20.9), Cu (89.5 and 58.4), Ni (49.8 and 19.6), Pb (32.1 and 12.1), and Zn (99.5 and 46.6). Ranges of translocation factors, root and shoot bioaccumulation factors were 0.21-3.49, 0.01-0.89 and 0.01-0.51, respectively. Overall, the binary chelant treatments were less toxic for R. communis growth and enhanced metal accumulation in shoots to a greater extent than the single chelant scenarios, but more so when EDTA was present in the binary combination. This suggests that the mixed chelants could be considered as alternative treatments for enhanced phytoextraction and revegetation of degraded waste dump soils.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.1
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• pp.55-59
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• 1989

Sodium methyl arsenate has been evaluated as a male sterilizing agent for the system of producing hybrid rice seeds. The compound was the most effective at the concentration of 0.02％. When applied as a foliar spray to four rice varieties at 15 days before heading, sodium methyl arsenate has produced 99％ male sterility. But the most effective time for application of the compound was 5 days before heading because of its phytotoxic effects. Effective application volume of the compound solution has depended on the growth of the plants treated. Varietal difference on the activity of the compound has been detected.

• Research in Plant Disease
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• v.15 no.2
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• pp.120-126
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• 2009

In the course of study on the roles of phytotoxins in the pathogenicity of Botrytis cinerea, we isolated two novel phytotoxins. They were identified as 3-O-acetyl botcinol and 3-O-acetyl botcinolide. In this study, we investigated correlation between the two phytotoxins and the pathogenicity of B. cinerea. In liquid cultures, the two phytotoxins were not produced by three low pathogenic isolates out of 25 B. cinerea isolates. Among strong or moderate pathogenic isolates, some produced the two phytotoxins, but the others did not. On the other hand, the ethyl acetate extracts of fermentation broths of 10 out of 25 isolates showed phytotoxic activity against various plants tested in a whole plant assay. The phytotoxins were detected in all of the 10 phytotoxic ethyl acetate extracts. In planta, the two phytotoxins were detected in all of the plant tissues infected with strong pathogenic isolates. However, there was no correlation between the ability of B. cinerea isolates to produce the two phytotoxins and their pathogenicities. The two phytotoxins began to detect in tomato plant tissues infected with B. cinerea 2-16 at 3 days after inoculation, increased gradually till 4 days after inoculation, and then decreased. The above results suggest that 3-O-acetyl botcinol and 3-O-acetyl botcinolide are one of pathogenicity factors for B. cinerea, but not a primary determinant of its pathogenicity.

• Korean journal of applied entomology
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• v.16 no.1 s.30
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• pp.41-45
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• 1977

The systemic insecticides carbofuran $(Curaterr\; 3\%\; G)$ mephosfolan $(Cytrolane\; 2\%\; G)$ and disulfoton$(Disyston\; 5\%\; G)$ were evaluated in the field for conrol of some insects on soybeans when the insecticides were applied in seeding-pits at the rates of 4.6, 9.2 and 13.8 mg(a.i)/pit at the planting time. Control of the soybean aphid (Aphis glycines Matsumura) and the two-spotted spider mite (Tetranychus telarius(L.)) on soybeans was obtained for about 2 to 3 months, but their treatments were ineffective against the soybean moth (Grapholitha glycinivorella Matsumura). For the control of aphids and mites, disulfoton was better than carbofuran and mephosfolan. The insecticides slightly reduced the soybean stands, while the low-dose treatments of carbofuran slightly increased the stands. Carbofuran and mephosfolan caused severe phytotoxicity at the early stages, but disulfoton showed slight or negligible phytotoxicity. The phytotoxic symptoms in carbofuran and mephosfolan treatments were shelving the large number of brown or black-brown spots on the cotyledon and the first-leaves, and in addition to that showing necrosis along the leaf-margin. The symptoms in disulfoton treatments were slight withering along the leaf-margin of the first-leaves. In spite of good control of aphids and mites, there were no differences in soybean yield.

• Korean Journal of Environmental Agriculture
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• v.1 no.2
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• pp.99-104
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• 1982

These studies were carried out to investigate the damage of root rot of hop by heptachlor residues in soil at nine farm fields and pot experiment for two years from 1980 to 1981. 1) Pot experiment results indicated that root rot of hop occurred in farm field were caused by heptachlor phytotoxicity. 2) The damage of root rot of the hop was found by heptachlor residues in soil. Hop root in the low concentration of heptachlor was turned to brown mottle, and then rotted. In high concentration, the hop root was decreased in number, blocked in growth, and resulted to greyish change with death. 3) Hop vine damaged by heptachlor was hardened and broken with ease. 4) Heptachlor epoxide which was inferred to be the main cause of hop root rot gave the damage to hop at 0.009 ppm reisdues in soil. 5) The phytotoxicity of heptachlor was proved to last for 10 years or more in this study.

• Journal of Soil and Groundwater Environment
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• v.6 no.4
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• pp.3-11
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• 2001

To select a suitable indigenous plant for the phytoremediation of TNT contaminated soil, eight representative species of native grasses were tested to identify TNT toxicity thresholds. The threshold was determined based on various factors including cumulative seed germination, root and shoot length, fresh biomass, and the amount of water uptake under various TNT concentrations. Phytotoxic effect of TNT on plants was increased with the increase in TNT concentration but the degree was varied between grass species. Concentrations up to 60-80mgTNT/liter did not affect germination of Abutilion avicennae, Echinochioa crusgalli var. frumentacea, and Aeschynomene indica. Phytotoxicity threshold inhibition (50%) of Abutilion avicennae, schinochioa crusgalli var. frumentacea, Aeschynomene indica were 5-40mgTNT/liter for root length, 50-73mg TNT/Liter for shoot length and 68-99mgTNT/Liter for fresh biomass during 14 days of seedling exposure. Root and shoot growth as well as fresh biomass decreased as TNT concentration increased. In addition, the amount of water uptake decreased with increasing TNT concentration in Abutilion avicennae and Aeschynomene indica. Comparison of toxicity thresholds for the tested grass species showed that sensitivity of plants to TNT was in the order of root length > shoot length > fresh biomass > germination rate. From these results, we concluded that Abutilion avicennae and Aeschynomene indica had tolerance to TNT among the species tested.

• Korean Journal of Weed Science
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• v.17 no.3
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• pp.281-287
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• 1997

Growth and anatomical responses of rice and barnyardgrass(Echinochloa crus-galli) to oxyfluorfen were examined under various growing conditions in a greenhouse. With foliar application at 0.08kg ai/ha 7 days after seeding or transplanting, oxyfluorfen completely reduced the shoot and root growth of barnyardgrass under all growing conditions. However, oxyfluorfen reduced the growth of direct seeded and transplanted rice by 0~64% and 0~12%, respectively. Phytotoxic effect on rice growth, with respect to plant height and shoot fresh weight, was more pronounced under water(53~63%) than under dry-condition(16~33%), and in broadcast-seeded(34~62%) than in drill-seeded rice(16~33%). Anatomical changes of epidermal and vascular bundle sheath cells of rice leaves were not induced by oxyfluorfen, but mesophyll cells were partially ruptured and total leaf thickness was reduced. Oxyfluorfen, however, constricted and ruptured mesophyll and vascular bundle sheath cells and severely reduced leaf thickness of barnyardgrass under all growing conditions examined. These anatomical changes were more severe under dry- than under water-seeded condition.

• Korean Journal of Weed Science
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• v.11 no.3
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• pp.187-194
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• 1991

Absorption and translocation, and metabolism studies using $^{14}C$-naproanilide were conducted to determine selective mode of action of naproanilide in rice and paddy weeds under different temperature conditions. Absorption amount of $^{14}C$-naproanilide was greater in Cyperus serotinus and Sagittaria pygmaea than rice and Echinochloa crusgalli. Especially, absorption of $^{14}C$-naproanilide in C. serotinus was increased twice at 32 $^{\circ}C$ and 48 hour exposure conditions. $^{14}C$-naproanilide in roots was translocated to shoots very little in rice and E. crusgalli, but S. pygmaea somewhat greater translocation than the other species. In C. serotinus and S. pygmaea, susceptible weeds, metabolic rates of naproanilide into phytotoxic NOP (2-(2-naphthoxy)-propionic acid) and NOPM (methyl 2-(2-naphthoxy) propionate) were significantly greater than in rice and E. crugalli, tolerant species. Consequently, differential uptake by roots and the difference in activation metabolism of naproanilide among species may explain the possible mechanism of selectivity.

• Korean Journal of Environmental Agriculture
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• v.12 no.2
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• pp.162-168
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• 1993

This research was conducted to assess the influence of humic or fulvic acid on Bentazone phytotoxicity using a bioassay with hydroponically grown cabbage (Brassica campestris subsp. napus var. pekinensis Makino). Concentrations of Bentazone in the water culture media were ranged from 0 to 32 ${\mu}M $ and those of the organic ligands were 1.0mM as a soluble carbon. Media were prepared in a complete factorial combination with pHs of 4.5, 6.5 and 8.5. The phytotoxicity indices on growth rate and dry weight decrement were employed to evaluate the effects of organic ligands on the Bentazone phytotoxicity. Humic or fulvic acid without Bentazone treatment enhanced the growth of cabbage and this effect was evident at low pH of 4.5. Bentazone led to chlorosis and necrosis on cabbage leaves resulting in the decreases of dry and fresh weights and growth rate. This phytotoxic effect was increased with Bentazone concentration and evident at low pH. At pH 4.5, dry weight was decreased about 63% with 8${\mu}$M of Bentazone treatment. Effective concentration of Bentazone causing 50% decreases in fresh weight as compared to the control was estimated to be 21${\mu}$M. Presence of organic ligand reduced the phytotoxicity of Bentazone to cabbage significantly by increasing yields and growth rates as compared to the treatment of Bentazone alone. At pH 4.5, fulvic acid reduced phytotoxicity of Bentazone upto 46%, and this efficiency of fulvic acid was better than that of humic acid under the same condition.